Job ID = 6627417 SRX = SRX8521381 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T02:21:32 prefetch.2.10.7: 1) Downloading 'SRR11978254'... 2020-07-14T02:21:32 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:21:51 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:21:51 prefetch.2.10.7: 'SRR11978254' is valid 2020-07-14T02:21:51 prefetch.2.10.7: 1) 'SRR11978254' was downloaded successfully 2020-07-14T02:21:51 prefetch.2.10.7: 'SRR11978254' has 0 unresolved dependencies Read 705576 spots for SRR11978254/SRR11978254.sra Written 705576 spots for SRR11978254/SRR11978254.sra 2020-07-14T02:22:17 prefetch.2.10.7: 1) Downloading 'SRR11978255'... 2020-07-14T02:22:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:22:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:22:41 prefetch.2.10.7: 'SRR11978255' is valid 2020-07-14T02:22:41 prefetch.2.10.7: 1) 'SRR11978255' was downloaded successfully 2020-07-14T02:22:41 prefetch.2.10.7: 'SRR11978255' has 0 unresolved dependencies Read 1715690 spots for SRR11978255/SRR11978255.sra Written 1715690 spots for SRR11978255/SRR11978255.sra 2020-07-14T02:23:03 prefetch.2.10.7: 1) Downloading 'SRR11978256'... 2020-07-14T02:23:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:23:28 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:23:29 prefetch.2.10.7: 'SRR11978256' is valid 2020-07-14T02:23:29 prefetch.2.10.7: 1) 'SRR11978256' was downloaded successfully 2020-07-14T02:23:29 prefetch.2.10.7: 'SRR11978256' has 0 unresolved dependencies Read 1817311 spots for SRR11978256/SRR11978256.sra Written 1817311 spots for SRR11978256/SRR11978256.sra 2020-07-14T02:23:49 prefetch.2.10.7: 1) Downloading 'SRR11978257'... 2020-07-14T02:23:49 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:24:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:24:13 prefetch.2.10.7: 'SRR11978257' is valid 2020-07-14T02:24:13 prefetch.2.10.7: 1) 'SRR11978257' was downloaded successfully 2020-07-14T02:24:13 prefetch.2.10.7: 'SRR11978257' has 0 unresolved dependencies Read 1777101 spots for SRR11978257/SRR11978257.sra Written 1777101 spots for SRR11978257/SRR11978257.sra 2020-07-14T02:24:30 prefetch.2.10.7: 1) Downloading 'SRR11978258'... 2020-07-14T02:24:30 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:24:58 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:24:59 prefetch.2.10.7: 'SRR11978258' is valid 2020-07-14T02:24:59 prefetch.2.10.7: 1) 'SRR11978258' was downloaded successfully 2020-07-14T02:24:59 prefetch.2.10.7: 'SRR11978258' has 0 unresolved dependencies Read 3012649 spots for SRR11978258/SRR11978258.sra Written 3012649 spots for SRR11978258/SRR11978258.sra 2020-07-14T02:25:23 prefetch.2.10.7: 1) Downloading 'SRR11978259'... 2020-07-14T02:25:23 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:25:54 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:25:55 prefetch.2.10.7: 'SRR11978259' is valid 2020-07-14T02:25:55 prefetch.2.10.7: 1) 'SRR11978259' was downloaded successfully 2020-07-14T02:25:55 prefetch.2.10.7: 'SRR11978259' has 0 unresolved dependencies Read 3043943 spots for SRR11978259/SRR11978259.sra Written 3043943 spots for SRR11978259/SRR11978259.sra 2020-07-14T02:26:19 prefetch.2.10.7: 1) Downloading 'SRR11978260'... 2020-07-14T02:26:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:26:52 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:26:52 prefetch.2.10.7: 'SRR11978260' is valid 2020-07-14T02:26:52 prefetch.2.10.7: 1) 'SRR11978260' was downloaded successfully 2020-07-14T02:26:52 prefetch.2.10.7: 'SRR11978260' has 0 unresolved dependencies Read 3049502 spots for SRR11978260/SRR11978260.sra Written 3049502 spots for SRR11978260/SRR11978260.sra 2020-07-14T02:27:15 prefetch.2.10.7: 1) Downloading 'SRR11978261'... 2020-07-14T02:27:15 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:27:43 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:27:43 prefetch.2.10.7: 'SRR11978261' is valid 2020-07-14T02:27:43 prefetch.2.10.7: 1) 'SRR11978261' was downloaded successfully 2020-07-14T02:27:43 prefetch.2.10.7: 'SRR11978261' has 0 unresolved dependencies Read 3030689 spots for SRR11978261/SRR11978261.sra Written 3030689 spots for SRR11978261/SRR11978261.sra 2020-07-14T02:28:04 prefetch.2.10.7: 1) Downloading 'SRR11978262'... 2020-07-14T02:28:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:28:24 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:28:24 prefetch.2.10.7: 'SRR11978262' is valid 2020-07-14T02:28:24 prefetch.2.10.7: 1) 'SRR11978262' was downloaded successfully 2020-07-14T02:28:24 prefetch.2.10.7: 'SRR11978262' has 0 unresolved dependencies Read 681132 spots for SRR11978262/SRR11978262.sra Written 681132 spots for SRR11978262/SRR11978262.sra 2020-07-14T02:28:41 prefetch.2.10.7: 1) Downloading 'SRR11978263'... 2020-07-14T02:28:41 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:28:57 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:28:57 prefetch.2.10.7: 'SRR11978263' is valid 2020-07-14T02:28:57 prefetch.2.10.7: 1) 'SRR11978263' was downloaded successfully 2020-07-14T02:28:57 prefetch.2.10.7: 'SRR11978263' has 0 unresolved dependencies Read 714212 spots for SRR11978263/SRR11978263.sra Written 714212 spots for SRR11978263/SRR11978263.sra 2020-07-14T02:29:15 prefetch.2.10.7: 1) Downloading 'SRR11978264'... 2020-07-14T02:29:15 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:29:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:29:59 prefetch.2.10.7: 'SRR11978264' is valid 2020-07-14T02:29:59 prefetch.2.10.7: 1) 'SRR11978264' was downloaded successfully 2020-07-14T02:29:59 prefetch.2.10.7: 'SRR11978264' has 0 unresolved dependencies Read 708405 spots for SRR11978264/SRR11978264.sra Written 708405 spots for SRR11978264/SRR11978264.sra 2020-07-14T02:30:12 prefetch.2.10.7: 1) Downloading 'SRR11978265'... 2020-07-14T02:30:12 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:31:05 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:31:05 prefetch.2.10.7: 'SRR11978265' is valid 2020-07-14T02:31:05 prefetch.2.10.7: 1) 'SRR11978265' was downloaded successfully 2020-07-14T02:31:05 prefetch.2.10.7: 'SRR11978265' has 0 unresolved dependencies Read 4998896 spots for SRR11978265/SRR11978265.sra Written 4998896 spots for SRR11978265/SRR11978265.sra 2020-07-14T02:31:36 prefetch.2.10.7: 1) Downloading 'SRR11978266'... 2020-07-14T02:31:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:32:21 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:32:22 prefetch.2.10.7: 'SRR11978266' is valid 2020-07-14T02:32:22 prefetch.2.10.7: 1) 'SRR11978266' was downloaded successfully 2020-07-14T02:32:22 prefetch.2.10.7: 'SRR11978266' has 0 unresolved dependencies Read 4894380 spots for SRR11978266/SRR11978266.sra Written 4894380 spots for SRR11978266/SRR11978266.sra 2020-07-14T02:33:19 prefetch.2.10.7: 1) Downloading 'SRR11978267'... 2020-07-14T02:33:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:34:05 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:34:06 prefetch.2.10.7: 'SRR11978267' is valid 2020-07-14T02:34:06 prefetch.2.10.7: 1) 'SRR11978267' was downloaded successfully 2020-07-14T02:34:06 prefetch.2.10.7: 'SRR11978267' has 0 unresolved dependencies Read 4999986 spots for SRR11978267/SRR11978267.sra Written 4999986 spots for SRR11978267/SRR11978267.sra 2020-07-14T02:34:33 prefetch.2.10.7: 1) Downloading 'SRR11978268'... 2020-07-14T02:34:33 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:35:26 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:35:26 prefetch.2.10.7: 'SRR11978268' is valid 2020-07-14T02:35:26 prefetch.2.10.7: 1) 'SRR11978268' was downloaded successfully 2020-07-14T02:35:26 prefetch.2.10.7: 'SRR11978268' has 0 unresolved dependencies Read 4962207 spots for SRR11978268/SRR11978268.sra Written 4962207 spots for SRR11978268/SRR11978268.sra 2020-07-14T02:35:56 prefetch.2.10.7: 1) Downloading 'SRR11978269'... 2020-07-14T02:35:56 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:36:49 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:36:49 prefetch.2.10.7: 'SRR11978269' is valid 2020-07-14T02:36:49 prefetch.2.10.7: 1) 'SRR11978269' was downloaded successfully 2020-07-14T02:36:49 prefetch.2.10.7: 'SRR11978269' has 0 unresolved dependencies Read 1771025 spots for SRR11978269/SRR11978269.sra Written 1771025 spots for SRR11978269/SRR11978269.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627624 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:29 41882704 reads; of these: 41882704 (100.00%) were unpaired; of these: 34389335 (82.11%) aligned 0 times 5761195 (13.76%) aligned exactly 1 time 1732174 (4.14%) aligned >1 times 17.89% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1503918 / 7493369 = 0.2007 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:47:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:47:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:47:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:47:16: 1000000 INFO @ Tue, 14 Jul 2020 11:47:23: 2000000 INFO @ Tue, 14 Jul 2020 11:47:31: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:47:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:47:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:47:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:47:38: 4000000 INFO @ Tue, 14 Jul 2020 11:47:46: 1000000 INFO @ Tue, 14 Jul 2020 11:47:47: 5000000 INFO @ Tue, 14 Jul 2020 11:47:53: 2000000 INFO @ Tue, 14 Jul 2020 11:47:54: #1 tag size is determined as 67 bps INFO @ Tue, 14 Jul 2020 11:47:54: #1 tag size = 67 INFO @ Tue, 14 Jul 2020 11:47:54: #1 total tags in treatment: 5989451 INFO @ Tue, 14 Jul 2020 11:47:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:47:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:47:54: #1 tags after filtering in treatment: 5989451 INFO @ Tue, 14 Jul 2020 11:47:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:47:54: #1 finished! INFO @ Tue, 14 Jul 2020 11:47:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:47:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:47:55: #2 number of paired peaks: 380 WARNING @ Tue, 14 Jul 2020 11:47:55: Fewer paired peaks (380) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 380 pairs to build model! INFO @ Tue, 14 Jul 2020 11:47:55: start model_add_line... INFO @ Tue, 14 Jul 2020 11:47:55: start X-correlation... INFO @ Tue, 14 Jul 2020 11:47:55: end of X-cor INFO @ Tue, 14 Jul 2020 11:47:55: #2 finished! INFO @ Tue, 14 Jul 2020 11:47:55: #2 predicted fragment length is 52 bps INFO @ Tue, 14 Jul 2020 11:47:55: #2 alternative fragment length(s) may be 52 bps INFO @ Tue, 14 Jul 2020 11:47:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.05_model.r WARNING @ Tue, 14 Jul 2020 11:47:55: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:47:55: #2 You may need to consider one of the other alternative d(s): 52 WARNING @ Tue, 14 Jul 2020 11:47:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:47:55: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:47:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:47:59: 3000000 INFO @ Tue, 14 Jul 2020 11:48:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:48:08: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:48:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:48:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:48:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:48:13: 5000000 INFO @ Tue, 14 Jul 2020 11:48:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:48:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:48:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.05_summits.bed INFO @ Tue, 14 Jul 2020 11:48:14: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1654 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:48:16: 1000000 INFO @ Tue, 14 Jul 2020 11:48:20: #1 tag size is determined as 67 bps INFO @ Tue, 14 Jul 2020 11:48:20: #1 tag size = 67 INFO @ Tue, 14 Jul 2020 11:48:20: #1 total tags in treatment: 5989451 INFO @ Tue, 14 Jul 2020 11:48:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:48:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:48:20: #1 tags after filtering in treatment: 5989451 INFO @ Tue, 14 Jul 2020 11:48:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:48:20: #1 finished! INFO @ Tue, 14 Jul 2020 11:48:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:48:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:48:20: #2 number of paired peaks: 380 WARNING @ Tue, 14 Jul 2020 11:48:20: Fewer paired peaks (380) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 380 pairs to build model! INFO @ Tue, 14 Jul 2020 11:48:20: start model_add_line... INFO @ Tue, 14 Jul 2020 11:48:20: start X-correlation... INFO @ Tue, 14 Jul 2020 11:48:20: end of X-cor INFO @ Tue, 14 Jul 2020 11:48:20: #2 finished! INFO @ Tue, 14 Jul 2020 11:48:20: #2 predicted fragment length is 52 bps INFO @ Tue, 14 Jul 2020 11:48:20: #2 alternative fragment length(s) may be 52 bps INFO @ Tue, 14 Jul 2020 11:48:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.10_model.r WARNING @ Tue, 14 Jul 2020 11:48:20: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:48:20: #2 You may need to consider one of the other alternative d(s): 52 WARNING @ Tue, 14 Jul 2020 11:48:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:48:20: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:48:20: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:48:24: 2000000 INFO @ Tue, 14 Jul 2020 11:48:32: 3000000 INFO @ Tue, 14 Jul 2020 11:48:33: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:48:39: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:48:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:48:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.10_summits.bed INFO @ Tue, 14 Jul 2020 11:48:39: Done! INFO @ Tue, 14 Jul 2020 11:48:39: 4000000 pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (719 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:48:46: 5000000 INFO @ Tue, 14 Jul 2020 11:48:54: #1 tag size is determined as 67 bps INFO @ Tue, 14 Jul 2020 11:48:54: #1 tag size = 67 INFO @ Tue, 14 Jul 2020 11:48:54: #1 total tags in treatment: 5989451 INFO @ Tue, 14 Jul 2020 11:48:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:48:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:48:54: #1 tags after filtering in treatment: 5989451 INFO @ Tue, 14 Jul 2020 11:48:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:48:54: #1 finished! INFO @ Tue, 14 Jul 2020 11:48:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:48:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:48:54: #2 number of paired peaks: 380 WARNING @ Tue, 14 Jul 2020 11:48:54: Fewer paired peaks (380) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 380 pairs to build model! INFO @ Tue, 14 Jul 2020 11:48:54: start model_add_line... INFO @ Tue, 14 Jul 2020 11:48:54: start X-correlation... INFO @ Tue, 14 Jul 2020 11:48:54: end of X-cor INFO @ Tue, 14 Jul 2020 11:48:54: #2 finished! INFO @ Tue, 14 Jul 2020 11:48:54: #2 predicted fragment length is 52 bps INFO @ Tue, 14 Jul 2020 11:48:54: #2 alternative fragment length(s) may be 52 bps INFO @ Tue, 14 Jul 2020 11:48:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.20_model.r WARNING @ Tue, 14 Jul 2020 11:48:54: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:48:54: #2 You may need to consider one of the other alternative d(s): 52 WARNING @ Tue, 14 Jul 2020 11:48:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:48:54: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:48:54: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:49:07: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:49:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:49:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:49:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521381/SRX8521381.20_summits.bed INFO @ Tue, 14 Jul 2020 11:49:13: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (147 records, 4 fields): 1 millis CompletedMACS2peakCalling