Job ID = 6627339 SRX = SRX8521372 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T02:00:21 prefetch.2.10.7: 1) Downloading 'SRR11978154'... 2020-07-14T02:00:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:00:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:00:59 prefetch.2.10.7: 'SRR11978154' is valid 2020-07-14T02:00:59 prefetch.2.10.7: 1) 'SRR11978154' was downloaded successfully 2020-07-14T02:00:59 prefetch.2.10.7: 'SRR11978154' has 0 unresolved dependencies Read 7704097 spots for SRR11978154/SRR11978154.sra Written 7704097 spots for SRR11978154/SRR11978154.sra 2020-07-14T02:01:35 prefetch.2.10.7: 1) Downloading 'SRR11978155'... 2020-07-14T02:01:35 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:03:09 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:03:09 prefetch.2.10.7: 'SRR11978155' is valid 2020-07-14T02:03:09 prefetch.2.10.7: 1) 'SRR11978155' was downloaded successfully 2020-07-14T02:03:09 prefetch.2.10.7: 'SRR11978155' has 0 unresolved dependencies Read 7554890 spots for SRR11978155/SRR11978155.sra Written 7554890 spots for SRR11978155/SRR11978155.sra 2020-07-14T02:03:45 prefetch.2.10.7: 1) Downloading 'SRR11978156'... 2020-07-14T02:03:45 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:04:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:04:41 prefetch.2.10.7: 'SRR11978156' is valid 2020-07-14T02:04:41 prefetch.2.10.7: 1) 'SRR11978156' was downloaded successfully 2020-07-14T02:04:41 prefetch.2.10.7: 'SRR11978156' has 0 unresolved dependencies Read 7731649 spots for SRR11978156/SRR11978156.sra Written 7731649 spots for SRR11978156/SRR11978156.sra 2020-07-14T02:05:17 prefetch.2.10.7: 1) Downloading 'SRR11978157'... 2020-07-14T02:05:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:06:18 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:06:18 prefetch.2.10.7: 'SRR11978157' is valid 2020-07-14T02:06:18 prefetch.2.10.7: 1) 'SRR11978157' was downloaded successfully 2020-07-14T02:06:18 prefetch.2.10.7: 'SRR11978157' has 0 unresolved dependencies Read 7642348 spots for SRR11978157/SRR11978157.sra Written 7642348 spots for SRR11978157/SRR11978157.sra 2020-07-14T02:06:54 prefetch.2.10.7: 1) Downloading 'SRR11978158'... 2020-07-14T02:06:54 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:09:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:09:50 prefetch.2.10.7: 1) 'SRR11978158' was downloaded successfully 2020-07-14T02:09:50 prefetch.2.10.7: 'SRR11978158' has 0 unresolved dependencies Read 22714572 spots for SRR11978158/SRR11978158.sra Written 22714572 spots for SRR11978158/SRR11978158.sra 2020-07-14T02:11:20 prefetch.2.10.7: 1) Downloading 'SRR11978159'... 2020-07-14T02:11:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:13:08 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:13:08 prefetch.2.10.7: 1) 'SRR11978159' was downloaded successfully 2020-07-14T02:13:08 prefetch.2.10.7: 'SRR11978159' has 0 unresolved dependencies Read 22096895 spots for SRR11978159/SRR11978159.sra Written 22096895 spots for SRR11978159/SRR11978159.sra 2020-07-14T02:14:36 prefetch.2.10.7: 1) Downloading 'SRR11978160'... 2020-07-14T02:14:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:17:02 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:17:02 prefetch.2.10.7: 1) 'SRR11978160' was downloaded successfully 2020-07-14T02:17:02 prefetch.2.10.7: 'SRR11978160' has 0 unresolved dependencies Read 23378308 spots for SRR11978160/SRR11978160.sra Written 23378308 spots for SRR11978160/SRR11978160.sra 2020-07-14T02:18:33 prefetch.2.10.7: 1) Downloading 'SRR11978161'... 2020-07-14T02:18:33 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:20:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:20:39 prefetch.2.10.7: 1) 'SRR11978161' was downloaded successfully 2020-07-14T02:20:39 prefetch.2.10.7: 'SRR11978161' has 0 unresolved dependencies Read 22916662 spots for SRR11978161/SRR11978161.sra Written 22916662 spots for SRR11978161/SRR11978161.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627639 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:58 121739421 reads; of these: 121739421 (100.00%) were unpaired; of these: 100131634 (82.25%) aligned 0 times 13491901 (11.08%) aligned exactly 1 time 8115886 (6.67%) aligned >1 times 17.75% overall alignment rate Time searching: 00:24:58 Overall time: 00:24:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7089745 / 21607787 = 0.3281 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:53:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:53:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:53:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:54:03: 1000000 INFO @ Tue, 14 Jul 2020 11:54:10: 2000000 INFO @ Tue, 14 Jul 2020 11:54:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:54:25: 4000000 INFO @ Tue, 14 Jul 2020 11:54:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:54:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:54:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:54:33: 5000000 INFO @ Tue, 14 Jul 2020 11:54:36: 1000000 INFO @ Tue, 14 Jul 2020 11:54:40: 6000000 INFO @ Tue, 14 Jul 2020 11:54:45: 2000000 INFO @ Tue, 14 Jul 2020 11:54:48: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:54:54: 3000000 INFO @ Tue, 14 Jul 2020 11:54:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:54:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:54:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:54:56: 8000000 INFO @ Tue, 14 Jul 2020 11:55:03: 1000000 INFO @ Tue, 14 Jul 2020 11:55:03: 4000000 INFO @ Tue, 14 Jul 2020 11:55:05: 9000000 INFO @ Tue, 14 Jul 2020 11:55:10: 2000000 INFO @ Tue, 14 Jul 2020 11:55:12: 5000000 INFO @ Tue, 14 Jul 2020 11:55:13: 10000000 INFO @ Tue, 14 Jul 2020 11:55:18: 3000000 INFO @ Tue, 14 Jul 2020 11:55:21: 6000000 INFO @ Tue, 14 Jul 2020 11:55:21: 11000000 INFO @ Tue, 14 Jul 2020 11:55:25: 4000000 INFO @ Tue, 14 Jul 2020 11:55:30: 12000000 INFO @ Tue, 14 Jul 2020 11:55:30: 7000000 INFO @ Tue, 14 Jul 2020 11:55:32: 5000000 INFO @ Tue, 14 Jul 2020 11:55:38: 13000000 INFO @ Tue, 14 Jul 2020 11:55:39: 8000000 INFO @ Tue, 14 Jul 2020 11:55:39: 6000000 INFO @ Tue, 14 Jul 2020 11:55:46: 14000000 INFO @ Tue, 14 Jul 2020 11:55:47: 7000000 INFO @ Tue, 14 Jul 2020 11:55:49: 9000000 INFO @ Tue, 14 Jul 2020 11:55:50: #1 tag size is determined as 70 bps INFO @ Tue, 14 Jul 2020 11:55:50: #1 tag size = 70 INFO @ Tue, 14 Jul 2020 11:55:50: #1 total tags in treatment: 14518042 INFO @ Tue, 14 Jul 2020 11:55:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:55:51: #1 tags after filtering in treatment: 14518042 INFO @ Tue, 14 Jul 2020 11:55:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:55:51: #1 finished! INFO @ Tue, 14 Jul 2020 11:55:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:55:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:55:52: #2 number of paired peaks: 61 WARNING @ Tue, 14 Jul 2020 11:55:52: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:55:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:55:54: 8000000 INFO @ Tue, 14 Jul 2020 11:55:58: 10000000 INFO @ Tue, 14 Jul 2020 11:56:01: 9000000 INFO @ Tue, 14 Jul 2020 11:56:08: 11000000 INFO @ Tue, 14 Jul 2020 11:56:08: 10000000 INFO @ Tue, 14 Jul 2020 11:56:15: 11000000 INFO @ Tue, 14 Jul 2020 11:56:18: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:56:22: 12000000 INFO @ Tue, 14 Jul 2020 11:56:27: 13000000 INFO @ Tue, 14 Jul 2020 11:56:30: 13000000 INFO @ Tue, 14 Jul 2020 11:56:36: 14000000 INFO @ Tue, 14 Jul 2020 11:56:37: 14000000 INFO @ Tue, 14 Jul 2020 11:56:41: #1 tag size is determined as 70 bps INFO @ Tue, 14 Jul 2020 11:56:41: #1 tag size = 70 INFO @ Tue, 14 Jul 2020 11:56:41: #1 total tags in treatment: 14518042 INFO @ Tue, 14 Jul 2020 11:56:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:56:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:56:41: #1 tag size is determined as 70 bps INFO @ Tue, 14 Jul 2020 11:56:41: #1 tag size = 70 INFO @ Tue, 14 Jul 2020 11:56:41: #1 total tags in treatment: 14518042 INFO @ Tue, 14 Jul 2020 11:56:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:56:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:56:41: #1 tags after filtering in treatment: 14518042 INFO @ Tue, 14 Jul 2020 11:56:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:56:41: #1 finished! INFO @ Tue, 14 Jul 2020 11:56:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:56:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:56:41: #1 tags after filtering in treatment: 14518042 INFO @ Tue, 14 Jul 2020 11:56:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:56:41: #1 finished! INFO @ Tue, 14 Jul 2020 11:56:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:56:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:56:42: #2 number of paired peaks: 61 WARNING @ Tue, 14 Jul 2020 11:56:42: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:56:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.20_peaks.narrowPeak: No such file or directory INFO @ Tue, 14 Jul 2020 11:56:42: #2 number of paired peaks: 61 WARNING @ Tue, 14 Jul 2020 11:56:42: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:56:42: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521372/SRX8521372.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。