Job ID = 6627301 SRX = SRX8521361 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:54:59 prefetch.2.10.7: 1) Downloading 'SRR11978098'... 2020-07-14T01:54:59 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:16 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:55:16 prefetch.2.10.7: 'SRR11978098' is valid 2020-07-14T01:55:16 prefetch.2.10.7: 1) 'SRR11978098' was downloaded successfully 2020-07-14T01:55:16 prefetch.2.10.7: 'SRR11978098' has 0 unresolved dependencies Read 248070 spots for SRR11978098/SRR11978098.sra Written 248070 spots for SRR11978098/SRR11978098.sra 2020-07-14T01:55:31 prefetch.2.10.7: 1) Downloading 'SRR11978099'... 2020-07-14T01:55:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:44 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:45 prefetch.2.10.7: 'SRR11978099' is valid 2020-07-14T01:56:45 prefetch.2.10.7: 1) 'SRR11978099' was downloaded successfully 2020-07-14T01:56:45 prefetch.2.10.7: 'SRR11978099' has 0 unresolved dependencies Read 6722506 spots for SRR11978099/SRR11978099.sra Written 6722506 spots for SRR11978099/SRR11978099.sra 2020-07-14T01:57:21 prefetch.2.10.7: 1) Downloading 'SRR11978100'... 2020-07-14T01:57:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:58:47 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:58:47 prefetch.2.10.7: 'SRR11978100' is valid 2020-07-14T01:58:47 prefetch.2.10.7: 1) 'SRR11978100' was downloaded successfully 2020-07-14T01:58:47 prefetch.2.10.7: 'SRR11978100' has 0 unresolved dependencies Read 7983139 spots for SRR11978100/SRR11978100.sra Written 7983139 spots for SRR11978100/SRR11978100.sra 2020-07-14T01:59:27 prefetch.2.10.7: 1) Downloading 'SRR11978101'... 2020-07-14T01:59:27 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:00:29 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:00:29 prefetch.2.10.7: 'SRR11978101' is valid 2020-07-14T02:00:29 prefetch.2.10.7: 1) 'SRR11978101' was downloaded successfully 2020-07-14T02:00:29 prefetch.2.10.7: 'SRR11978101' has 0 unresolved dependencies Read 7237476 spots for SRR11978101/SRR11978101.sra Written 7237476 spots for SRR11978101/SRR11978101.sra 2020-07-14T02:01:07 prefetch.2.10.7: 1) Downloading 'SRR11978102'... 2020-07-14T02:01:07 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:02:20 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:02:20 prefetch.2.10.7: 'SRR11978102' is valid 2020-07-14T02:02:20 prefetch.2.10.7: 1) 'SRR11978102' was downloaded successfully 2020-07-14T02:02:20 prefetch.2.10.7: 'SRR11978102' has 0 unresolved dependencies Read 4428291 spots for SRR11978102/SRR11978102.sra Written 4428291 spots for SRR11978102/SRR11978102.sra 2020-07-14T02:02:48 prefetch.2.10.7: 1) Downloading 'SRR11978103'... 2020-07-14T02:02:48 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:03:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:03:39 prefetch.2.10.7: 'SRR11978103' is valid 2020-07-14T02:03:39 prefetch.2.10.7: 1) 'SRR11978103' was downloaded successfully 2020-07-14T02:03:39 prefetch.2.10.7: 'SRR11978103' has 0 unresolved dependencies Read 3961119 spots for SRR11978103/SRR11978103.sra Written 3961119 spots for SRR11978103/SRR11978103.sra 2020-07-14T02:04:03 prefetch.2.10.7: 1) Downloading 'SRR11978104'... 2020-07-14T02:04:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:05:55 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:05:56 prefetch.2.10.7: 'SRR11978104' is valid 2020-07-14T02:05:56 prefetch.2.10.7: 1) 'SRR11978104' was downloaded successfully 2020-07-14T02:05:56 prefetch.2.10.7: 'SRR11978104' has 0 unresolved dependencies Read 4475729 spots for SRR11978104/SRR11978104.sra Written 4475729 spots for SRR11978104/SRR11978104.sra 2020-07-14T02:06:25 prefetch.2.10.7: 1) Downloading 'SRR11978105'... 2020-07-14T02:06:25 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:07:25 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:07:25 prefetch.2.10.7: 'SRR11978105' is valid 2020-07-14T02:07:25 prefetch.2.10.7: 1) 'SRR11978105' was downloaded successfully 2020-07-14T02:07:25 prefetch.2.10.7: 'SRR11978105' has 0 unresolved dependencies Read 3645445 spots for SRR11978105/SRR11978105.sra Written 3645445 spots for SRR11978105/SRR11978105.sra 2020-07-14T02:07:47 prefetch.2.10.7: 1) Downloading 'SRR11978106'... 2020-07-14T02:07:47 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:07:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:07:59 prefetch.2.10.7: 'SRR11978106' is valid 2020-07-14T02:07:59 prefetch.2.10.7: 1) 'SRR11978106' was downloaded successfully 2020-07-14T02:07:59 prefetch.2.10.7: 'SRR11978106' has 0 unresolved dependencies Read 172734 spots for SRR11978106/SRR11978106.sra Written 172734 spots for SRR11978106/SRR11978106.sra 2020-07-14T02:08:10 prefetch.2.10.7: 1) Downloading 'SRR11978107'... 2020-07-14T02:08:10 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:08:26 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:08:26 prefetch.2.10.7: 'SRR11978107' is valid 2020-07-14T02:08:26 prefetch.2.10.7: 1) 'SRR11978107' was downloaded successfully 2020-07-14T02:08:26 prefetch.2.10.7: 'SRR11978107' has 0 unresolved dependencies Read 248851 spots for SRR11978107/SRR11978107.sra Written 248851 spots for SRR11978107/SRR11978107.sra 2020-07-14T02:08:38 prefetch.2.10.7: 1) Downloading 'SRR11978108'... 2020-07-14T02:08:38 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:08:58 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:08:58 prefetch.2.10.7: 'SRR11978108' is valid 2020-07-14T02:08:58 prefetch.2.10.7: 1) 'SRR11978108' was downloaded successfully 2020-07-14T02:08:58 prefetch.2.10.7: 'SRR11978108' has 0 unresolved dependencies Read 191212 spots for SRR11978108/SRR11978108.sra Written 191212 spots for SRR11978108/SRR11978108.sra 2020-07-14T02:09:13 prefetch.2.10.7: 1) Downloading 'SRR11978109'... 2020-07-14T02:09:13 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:09:35 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:09:35 prefetch.2.10.7: 'SRR11978109' is valid 2020-07-14T02:09:35 prefetch.2.10.7: 1) 'SRR11978109' was downloaded successfully 2020-07-14T02:09:35 prefetch.2.10.7: 'SRR11978109' has 0 unresolved dependencies Read 1718882 spots for SRR11978109/SRR11978109.sra Written 1718882 spots for SRR11978109/SRR11978109.sra 2020-07-14T02:09:56 prefetch.2.10.7: 1) Downloading 'SRR11978110'... 2020-07-14T02:09:56 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:10:24 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:10:24 prefetch.2.10.7: 'SRR11978110' is valid 2020-07-14T02:10:24 prefetch.2.10.7: 1) 'SRR11978110' was downloaded successfully 2020-07-14T02:10:24 prefetch.2.10.7: 'SRR11978110' has 0 unresolved dependencies Read 1374341 spots for SRR11978110/SRR11978110.sra Written 1374341 spots for SRR11978110/SRR11978110.sra 2020-07-14T02:10:43 prefetch.2.10.7: 1) Downloading 'SRR11978111'... 2020-07-14T02:10:43 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:11:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:11:10 prefetch.2.10.7: 'SRR11978111' is valid 2020-07-14T02:11:10 prefetch.2.10.7: 1) 'SRR11978111' was downloaded successfully 2020-07-14T02:11:10 prefetch.2.10.7: 'SRR11978111' has 0 unresolved dependencies Read 1642634 spots for SRR11978111/SRR11978111.sra Written 1642634 spots for SRR11978111/SRR11978111.sra 2020-07-14T02:11:26 prefetch.2.10.7: 1) Downloading 'SRR11978112'... 2020-07-14T02:11:26 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:11:46 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:11:46 prefetch.2.10.7: 'SRR11978112' is valid 2020-07-14T02:11:46 prefetch.2.10.7: 1) 'SRR11978112' was downloaded successfully 2020-07-14T02:11:46 prefetch.2.10.7: 'SRR11978112' has 0 unresolved dependencies Read 1447507 spots for SRR11978112/SRR11978112.sra Written 1447507 spots for SRR11978112/SRR11978112.sra 2020-07-14T02:12:05 prefetch.2.10.7: 1) Downloading 'SRR11978113'... 2020-07-14T02:12:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:13:00 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:13:00 prefetch.2.10.7: 'SRR11978113' is valid 2020-07-14T02:13:00 prefetch.2.10.7: 1) 'SRR11978113' was downloaded successfully 2020-07-14T02:13:00 prefetch.2.10.7: 'SRR11978113' has 0 unresolved dependencies Read 8348315 spots for SRR11978113/SRR11978113.sra Written 8348315 spots for SRR11978113/SRR11978113.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627568 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:49 53846251 reads; of these: 53846251 (100.00%) were unpaired; of these: 41896651 (77.81%) aligned 0 times 8953964 (16.63%) aligned exactly 1 time 2995636 (5.56%) aligned >1 times 22.19% overall alignment rate Time searching: 00:08:49 Overall time: 00:08:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2464336 / 11949600 = 0.2062 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:26:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:26:36: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:26:36: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:26:44: 1000000 INFO @ Tue, 14 Jul 2020 11:26:51: 2000000 INFO @ Tue, 14 Jul 2020 11:26:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:27:05: 4000000 INFO @ Tue, 14 Jul 2020 11:27:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:27:06: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:27:06: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:27:13: 5000000 INFO @ Tue, 14 Jul 2020 11:27:13: 1000000 INFO @ Tue, 14 Jul 2020 11:27:20: 2000000 INFO @ Tue, 14 Jul 2020 11:27:21: 6000000 INFO @ Tue, 14 Jul 2020 11:27:27: 3000000 INFO @ Tue, 14 Jul 2020 11:27:28: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:27:35: 4000000 INFO @ Tue, 14 Jul 2020 11:27:36: 8000000 INFO @ Tue, 14 Jul 2020 11:27:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:27:36: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:27:36: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:27:42: 5000000 INFO @ Tue, 14 Jul 2020 11:27:44: 1000000 INFO @ Tue, 14 Jul 2020 11:27:44: 9000000 INFO @ Tue, 14 Jul 2020 11:27:47: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 11:27:47: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 11:27:47: #1 total tags in treatment: 9485264 INFO @ Tue, 14 Jul 2020 11:27:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:27:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:27:47: #1 tags after filtering in treatment: 9485264 INFO @ Tue, 14 Jul 2020 11:27:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:27:47: #1 finished! INFO @ Tue, 14 Jul 2020 11:27:47: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:27:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:27:48: #2 number of paired peaks: 246 WARNING @ Tue, 14 Jul 2020 11:27:48: Fewer paired peaks (246) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 246 pairs to build model! INFO @ Tue, 14 Jul 2020 11:27:48: start model_add_line... INFO @ Tue, 14 Jul 2020 11:27:48: start X-correlation... INFO @ Tue, 14 Jul 2020 11:27:48: end of X-cor INFO @ Tue, 14 Jul 2020 11:27:48: #2 finished! INFO @ Tue, 14 Jul 2020 11:27:48: #2 predicted fragment length is 61 bps INFO @ Tue, 14 Jul 2020 11:27:48: #2 alternative fragment length(s) may be 61 bps INFO @ Tue, 14 Jul 2020 11:27:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.05_model.r WARNING @ Tue, 14 Jul 2020 11:27:48: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:27:48: #2 You may need to consider one of the other alternative d(s): 61 WARNING @ Tue, 14 Jul 2020 11:27:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:27:48: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:27:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:27:49: 6000000 INFO @ Tue, 14 Jul 2020 11:27:51: 2000000 INFO @ Tue, 14 Jul 2020 11:27:56: 7000000 INFO @ Tue, 14 Jul 2020 11:27:58: 3000000 INFO @ Tue, 14 Jul 2020 11:28:03: 8000000 INFO @ Tue, 14 Jul 2020 11:28:05: 4000000 INFO @ Tue, 14 Jul 2020 11:28:08: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:28:10: 9000000 INFO @ Tue, 14 Jul 2020 11:28:12: 5000000 INFO @ Tue, 14 Jul 2020 11:28:13: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 11:28:13: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 11:28:13: #1 total tags in treatment: 9485264 INFO @ Tue, 14 Jul 2020 11:28:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:28:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:28:13: #1 tags after filtering in treatment: 9485264 INFO @ Tue, 14 Jul 2020 11:28:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:28:13: #1 finished! INFO @ Tue, 14 Jul 2020 11:28:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:28:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:28:14: #2 number of paired peaks: 246 WARNING @ Tue, 14 Jul 2020 11:28:14: Fewer paired peaks (246) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 246 pairs to build model! INFO @ Tue, 14 Jul 2020 11:28:14: start model_add_line... INFO @ Tue, 14 Jul 2020 11:28:14: start X-correlation... INFO @ Tue, 14 Jul 2020 11:28:14: end of X-cor INFO @ Tue, 14 Jul 2020 11:28:14: #2 finished! INFO @ Tue, 14 Jul 2020 11:28:14: #2 predicted fragment length is 61 bps INFO @ Tue, 14 Jul 2020 11:28:14: #2 alternative fragment length(s) may be 61 bps INFO @ Tue, 14 Jul 2020 11:28:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.10_model.r WARNING @ Tue, 14 Jul 2020 11:28:14: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:28:14: #2 You may need to consider one of the other alternative d(s): 61 WARNING @ Tue, 14 Jul 2020 11:28:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:28:14: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:28:14: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:28:17: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:28:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:28:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.05_summits.bed INFO @ Tue, 14 Jul 2020 11:28:17: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2902 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:28:19: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:28:26: 7000000 INFO @ Tue, 14 Jul 2020 11:28:33: 8000000 INFO @ Tue, 14 Jul 2020 11:28:33: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:28:39: 9000000 INFO @ Tue, 14 Jul 2020 11:28:42: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 11:28:42: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 11:28:42: #1 total tags in treatment: 9485264 INFO @ Tue, 14 Jul 2020 11:28:42: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:28:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:28:42: #1 tags after filtering in treatment: 9485264 INFO @ Tue, 14 Jul 2020 11:28:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:28:42: #1 finished! INFO @ Tue, 14 Jul 2020 11:28:42: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:28:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:28:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:28:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:28:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.10_summits.bed INFO @ Tue, 14 Jul 2020 11:28:43: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1174 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:28:43: #2 number of paired peaks: 246 WARNING @ Tue, 14 Jul 2020 11:28:43: Fewer paired peaks (246) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 246 pairs to build model! INFO @ Tue, 14 Jul 2020 11:28:43: start model_add_line... INFO @ Tue, 14 Jul 2020 11:28:43: start X-correlation... INFO @ Tue, 14 Jul 2020 11:28:43: end of X-cor INFO @ Tue, 14 Jul 2020 11:28:43: #2 finished! INFO @ Tue, 14 Jul 2020 11:28:43: #2 predicted fragment length is 61 bps INFO @ Tue, 14 Jul 2020 11:28:43: #2 alternative fragment length(s) may be 61 bps INFO @ Tue, 14 Jul 2020 11:28:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.20_model.r WARNING @ Tue, 14 Jul 2020 11:28:43: #2 Since the d (61) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:28:43: #2 You may need to consider one of the other alternative d(s): 61 WARNING @ Tue, 14 Jul 2020 11:28:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:28:43: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:28:43: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:29:02: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:29:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:29:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:29:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521361/SRX8521361.20_summits.bed INFO @ Tue, 14 Jul 2020 11:29:12: Done! pass1 - making usageList (9 chroms): 2 millis pass2 - checking and writing primary data (230 records, 4 fields): 1 millis CompletedMACS2peakCalling