Job ID = 6627279 SRX = SRX8521345 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:48:56 prefetch.2.10.7: 1) Downloading 'SRR11978608'... 2020-07-14T01:48:56 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:20 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:20 prefetch.2.10.7: 'SRR11978608' is valid 2020-07-14T01:49:20 prefetch.2.10.7: 1) 'SRR11978608' was downloaded successfully 2020-07-14T01:49:20 prefetch.2.10.7: 'SRR11978608' has 0 unresolved dependencies Read 563555 spots for SRR11978608/SRR11978608.sra Written 563555 spots for SRR11978608/SRR11978608.sra 2020-07-14T01:49:34 prefetch.2.10.7: 1) Downloading 'SRR11978609'... 2020-07-14T01:49:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:51 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:51 prefetch.2.10.7: 'SRR11978609' is valid 2020-07-14T01:49:51 prefetch.2.10.7: 1) 'SRR11978609' was downloaded successfully 2020-07-14T01:49:51 prefetch.2.10.7: 'SRR11978609' has 0 unresolved dependencies Read 982589 spots for SRR11978609/SRR11978609.sra Written 982589 spots for SRR11978609/SRR11978609.sra 2020-07-14T01:50:05 prefetch.2.10.7: 1) Downloading 'SRR11978610'... 2020-07-14T01:50:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:50:26 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:50:26 prefetch.2.10.7: 'SRR11978610' is valid 2020-07-14T01:50:26 prefetch.2.10.7: 1) 'SRR11978610' was downloaded successfully 2020-07-14T01:50:26 prefetch.2.10.7: 'SRR11978610' has 0 unresolved dependencies Read 1047962 spots for SRR11978610/SRR11978610.sra Written 1047962 spots for SRR11978610/SRR11978610.sra 2020-07-14T01:50:52 prefetch.2.10.7: 1) Downloading 'SRR11978611'... 2020-07-14T01:50:52 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:51:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:51:13 prefetch.2.10.7: 'SRR11978611' is valid 2020-07-14T01:51:13 prefetch.2.10.7: 1) 'SRR11978611' was downloaded successfully 2020-07-14T01:51:13 prefetch.2.10.7: 'SRR11978611' has 0 unresolved dependencies Read 1019877 spots for SRR11978611/SRR11978611.sra Written 1019877 spots for SRR11978611/SRR11978611.sra 2020-07-14T01:51:28 prefetch.2.10.7: 1) Downloading 'SRR11978612'... 2020-07-14T01:51:28 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:51:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:51:50 prefetch.2.10.7: 'SRR11978612' is valid 2020-07-14T01:51:50 prefetch.2.10.7: 1) 'SRR11978612' was downloaded successfully 2020-07-14T01:51:50 prefetch.2.10.7: 'SRR11978612' has 0 unresolved dependencies Read 1258340 spots for SRR11978612/SRR11978612.sra Written 1258340 spots for SRR11978612/SRR11978612.sra 2020-07-14T01:52:05 prefetch.2.10.7: 1) Downloading 'SRR11978613'... 2020-07-14T01:52:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:52:26 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:52:26 prefetch.2.10.7: 'SRR11978613' is valid 2020-07-14T01:52:26 prefetch.2.10.7: 1) 'SRR11978613' was downloaded successfully 2020-07-14T01:52:26 prefetch.2.10.7: 'SRR11978613' has 0 unresolved dependencies Read 1277241 spots for SRR11978613/SRR11978613.sra Written 1277241 spots for SRR11978613/SRR11978613.sra 2020-07-14T01:52:46 prefetch.2.10.7: 1) Downloading 'SRR11978614'... 2020-07-14T01:52:46 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:53:07 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:53:07 prefetch.2.10.7: 'SRR11978614' is valid 2020-07-14T01:53:07 prefetch.2.10.7: 1) 'SRR11978614' was downloaded successfully 2020-07-14T01:53:07 prefetch.2.10.7: 'SRR11978614' has 0 unresolved dependencies Read 1284646 spots for SRR11978614/SRR11978614.sra Written 1284646 spots for SRR11978614/SRR11978614.sra 2020-07-14T01:53:22 prefetch.2.10.7: 1) Downloading 'SRR11978615'... 2020-07-14T01:53:22 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:53:49 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:53:49 prefetch.2.10.7: 'SRR11978615' is valid 2020-07-14T01:53:49 prefetch.2.10.7: 1) 'SRR11978615' was downloaded successfully 2020-07-14T01:53:49 prefetch.2.10.7: 'SRR11978615' has 0 unresolved dependencies Read 1266097 spots for SRR11978615/SRR11978615.sra Written 1266097 spots for SRR11978615/SRR11978615.sra 2020-07-14T01:54:08 prefetch.2.10.7: 1) Downloading 'SRR11978616'... 2020-07-14T01:54:08 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:54:27 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:54:27 prefetch.2.10.7: 'SRR11978616' is valid 2020-07-14T01:54:27 prefetch.2.10.7: 1) 'SRR11978616' was downloaded successfully 2020-07-14T01:54:27 prefetch.2.10.7: 'SRR11978616' has 0 unresolved dependencies Read 546045 spots for SRR11978616/SRR11978616.sra Written 546045 spots for SRR11978616/SRR11978616.sra 2020-07-14T01:54:44 prefetch.2.10.7: 1) Downloading 'SRR11978617'... 2020-07-14T01:54:44 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:04 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:55:04 prefetch.2.10.7: 'SRR11978617' is valid 2020-07-14T01:55:04 prefetch.2.10.7: 1) 'SRR11978617' was downloaded successfully 2020-07-14T01:55:04 prefetch.2.10.7: 'SRR11978617' has 0 unresolved dependencies Read 576325 spots for SRR11978617/SRR11978617.sra Written 576325 spots for SRR11978617/SRR11978617.sra 2020-07-14T01:55:25 prefetch.2.10.7: 1) Downloading 'SRR11978618'... 2020-07-14T01:55:25 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:45 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:55:45 prefetch.2.10.7: 'SRR11978618' is valid 2020-07-14T01:55:45 prefetch.2.10.7: 1) 'SRR11978618' was downloaded successfully 2020-07-14T01:55:45 prefetch.2.10.7: 'SRR11978618' has 0 unresolved dependencies Read 571245 spots for SRR11978618/SRR11978618.sra Written 571245 spots for SRR11978618/SRR11978618.sra 2020-07-14T01:55:59 prefetch.2.10.7: 1) Downloading 'SRR11978619'... 2020-07-14T01:55:59 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:46 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:46 prefetch.2.10.7: 'SRR11978619' is valid 2020-07-14T01:56:46 prefetch.2.10.7: 1) 'SRR11978619' was downloaded successfully 2020-07-14T01:56:46 prefetch.2.10.7: 'SRR11978619' has 0 unresolved dependencies Read 6255131 spots for SRR11978619/SRR11978619.sra Written 6255131 spots for SRR11978619/SRR11978619.sra 2020-07-14T01:57:24 prefetch.2.10.7: 1) Downloading 'SRR11978620'... 2020-07-14T01:57:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:58:36 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:58:36 prefetch.2.10.7: 'SRR11978620' is valid 2020-07-14T01:58:36 prefetch.2.10.7: 1) 'SRR11978620' was downloaded successfully 2020-07-14T01:58:36 prefetch.2.10.7: 'SRR11978620' has 0 unresolved dependencies Read 6122833 spots for SRR11978620/SRR11978620.sra Written 6122833 spots for SRR11978620/SRR11978620.sra 2020-07-14T01:59:13 prefetch.2.10.7: 1) Downloading 'SRR11978621'... 2020-07-14T01:59:13 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:00:32 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:00:32 prefetch.2.10.7: 'SRR11978621' is valid 2020-07-14T02:00:32 prefetch.2.10.7: 1) 'SRR11978621' was downloaded successfully 2020-07-14T02:00:32 prefetch.2.10.7: 'SRR11978621' has 0 unresolved dependencies Read 6351831 spots for SRR11978621/SRR11978621.sra Written 6351831 spots for SRR11978621/SRR11978621.sra 2020-07-14T02:01:10 prefetch.2.10.7: 1) Downloading 'SRR11978622'... 2020-07-14T02:01:10 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:02:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:02:11 prefetch.2.10.7: 'SRR11978622' is valid 2020-07-14T02:02:11 prefetch.2.10.7: 1) 'SRR11978622' was downloaded successfully 2020-07-14T02:02:11 prefetch.2.10.7: 'SRR11978622' has 0 unresolved dependencies Read 6338480 spots for SRR11978622/SRR11978622.sra Written 6338480 spots for SRR11978622/SRR11978622.sra 2020-07-14T02:03:04 prefetch.2.10.7: 1) Downloading 'SRR11978623'... 2020-07-14T02:03:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:05:12 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:05:12 prefetch.2.10.7: 'SRR11978623' is valid 2020-07-14T02:05:12 prefetch.2.10.7: 1) 'SRR11978623' was downloaded successfully 2020-07-14T02:05:12 prefetch.2.10.7: 'SRR11978623' has 0 unresolved dependencies Read 1012413 spots for SRR11978623/SRR11978623.sra Written 1012413 spots for SRR11978623/SRR11978623.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627527 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:32 36474610 reads; of these: 36474610 (100.00%) were unpaired; of these: 30672675 (84.09%) aligned 0 times 4483750 (12.29%) aligned exactly 1 time 1318185 (3.61%) aligned >1 times 15.91% overall alignment rate Time searching: 00:06:32 Overall time: 00:06:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1343493 / 5801935 = 0.2316 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:14:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:14:20: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:14:20: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:14:26: 1000000 INFO @ Tue, 14 Jul 2020 11:14:32: 2000000 INFO @ Tue, 14 Jul 2020 11:14:38: 3000000 INFO @ Tue, 14 Jul 2020 11:14:44: 4000000 INFO @ Tue, 14 Jul 2020 11:14:47: #1 tag size is determined as 65 bps INFO @ Tue, 14 Jul 2020 11:14:47: #1 tag size = 65 INFO @ Tue, 14 Jul 2020 11:14:47: #1 total tags in treatment: 4458442 INFO @ Tue, 14 Jul 2020 11:14:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:14:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:14:47: #1 tags after filtering in treatment: 4458442 INFO @ Tue, 14 Jul 2020 11:14:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:14:47: #1 finished! INFO @ Tue, 14 Jul 2020 11:14:47: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:14:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:14:47: #2 number of paired peaks: 485 WARNING @ Tue, 14 Jul 2020 11:14:47: Fewer paired peaks (485) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 485 pairs to build model! INFO @ Tue, 14 Jul 2020 11:14:47: start model_add_line... INFO @ Tue, 14 Jul 2020 11:14:47: start X-correlation... INFO @ Tue, 14 Jul 2020 11:14:47: end of X-cor INFO @ Tue, 14 Jul 2020 11:14:47: #2 finished! INFO @ Tue, 14 Jul 2020 11:14:47: #2 predicted fragment length is 70 bps INFO @ Tue, 14 Jul 2020 11:14:47: #2 alternative fragment length(s) may be 70 bps INFO @ Tue, 14 Jul 2020 11:14:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.05_model.r WARNING @ Tue, 14 Jul 2020 11:14:47: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:14:47: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Tue, 14 Jul 2020 11:14:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:14:47: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:14:47: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:14:50: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:14:50: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:14:57: 1000000 INFO @ Tue, 14 Jul 2020 11:14:58: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:15:03: 2000000 INFO @ Tue, 14 Jul 2020 11:15:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:15:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:15:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.05_summits.bed INFO @ Tue, 14 Jul 2020 11:15:03: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1518 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:15:09: 3000000 INFO @ Tue, 14 Jul 2020 11:15:15: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:15:18: #1 tag size is determined as 65 bps INFO @ Tue, 14 Jul 2020 11:15:18: #1 tag size = 65 INFO @ Tue, 14 Jul 2020 11:15:18: #1 total tags in treatment: 4458442 INFO @ Tue, 14 Jul 2020 11:15:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:15:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:15:18: #1 tags after filtering in treatment: 4458442 INFO @ Tue, 14 Jul 2020 11:15:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:15:18: #1 finished! INFO @ Tue, 14 Jul 2020 11:15:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:15:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:15:19: #2 number of paired peaks: 485 WARNING @ Tue, 14 Jul 2020 11:15:19: Fewer paired peaks (485) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 485 pairs to build model! INFO @ Tue, 14 Jul 2020 11:15:19: start model_add_line... INFO @ Tue, 14 Jul 2020 11:15:19: start X-correlation... INFO @ Tue, 14 Jul 2020 11:15:19: end of X-cor INFO @ Tue, 14 Jul 2020 11:15:19: #2 finished! INFO @ Tue, 14 Jul 2020 11:15:19: #2 predicted fragment length is 70 bps INFO @ Tue, 14 Jul 2020 11:15:19: #2 alternative fragment length(s) may be 70 bps INFO @ Tue, 14 Jul 2020 11:15:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.10_model.r WARNING @ Tue, 14 Jul 2020 11:15:19: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:15:19: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Tue, 14 Jul 2020 11:15:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:15:19: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:15:19: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:15:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:15:20: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:15:20: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:15:27: 1000000 INFO @ Tue, 14 Jul 2020 11:15:29: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:15:34: 2000000 INFO @ Tue, 14 Jul 2020 11:15:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:15:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:15:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.10_summits.bed INFO @ Tue, 14 Jul 2020 11:15:34: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (590 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:15:41: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:15:48: 4000000 INFO @ Tue, 14 Jul 2020 11:15:51: #1 tag size is determined as 65 bps INFO @ Tue, 14 Jul 2020 11:15:51: #1 tag size = 65 INFO @ Tue, 14 Jul 2020 11:15:51: #1 total tags in treatment: 4458442 INFO @ Tue, 14 Jul 2020 11:15:51: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:15:51: #1 tags after filtering in treatment: 4458442 INFO @ Tue, 14 Jul 2020 11:15:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:15:51: #1 finished! INFO @ Tue, 14 Jul 2020 11:15:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:15:52: #2 number of paired peaks: 485 WARNING @ Tue, 14 Jul 2020 11:15:52: Fewer paired peaks (485) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 485 pairs to build model! INFO @ Tue, 14 Jul 2020 11:15:52: start model_add_line... INFO @ Tue, 14 Jul 2020 11:15:52: start X-correlation... INFO @ Tue, 14 Jul 2020 11:15:52: end of X-cor INFO @ Tue, 14 Jul 2020 11:15:52: #2 finished! INFO @ Tue, 14 Jul 2020 11:15:52: #2 predicted fragment length is 70 bps INFO @ Tue, 14 Jul 2020 11:15:52: #2 alternative fragment length(s) may be 70 bps INFO @ Tue, 14 Jul 2020 11:15:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.20_model.r WARNING @ Tue, 14 Jul 2020 11:15:52: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:15:52: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Tue, 14 Jul 2020 11:15:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:15:52: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:15:52: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:16:03: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:16:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:16:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:16:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521345/SRX8521345.20_summits.bed INFO @ Tue, 14 Jul 2020 11:16:08: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (161 records, 4 fields): 1 millis CompletedMACS2peakCalling