Job ID = 6627269 SRX = SRX8521337 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:44:56 prefetch.2.10.7: 1) Downloading 'SRR11978544'... 2020-07-14T01:44:56 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:23 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:23 prefetch.2.10.7: 'SRR11978544' is valid 2020-07-14T01:45:23 prefetch.2.10.7: 1) 'SRR11978544' was downloaded successfully 2020-07-14T01:45:23 prefetch.2.10.7: 'SRR11978544' has 0 unresolved dependencies Read 820854 spots for SRR11978544/SRR11978544.sra Written 820854 spots for SRR11978544/SRR11978544.sra 2020-07-14T01:45:36 prefetch.2.10.7: 1) Downloading 'SRR11978545'... 2020-07-14T01:45:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:46:38 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:46:38 prefetch.2.10.7: 'SRR11978545' is valid 2020-07-14T01:46:38 prefetch.2.10.7: 1) 'SRR11978545' was downloaded successfully 2020-07-14T01:46:38 prefetch.2.10.7: 'SRR11978545' has 0 unresolved dependencies Read 5339549 spots for SRR11978545/SRR11978545.sra Written 5339549 spots for SRR11978545/SRR11978545.sra 2020-07-14T01:47:06 prefetch.2.10.7: 1) Downloading 'SRR11978546'... 2020-07-14T01:47:06 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:55 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:56 prefetch.2.10.7: 'SRR11978546' is valid 2020-07-14T01:47:56 prefetch.2.10.7: 1) 'SRR11978546' was downloaded successfully 2020-07-14T01:47:56 prefetch.2.10.7: 'SRR11978546' has 0 unresolved dependencies Read 5515348 spots for SRR11978546/SRR11978546.sra Written 5515348 spots for SRR11978546/SRR11978546.sra 2020-07-14T01:48:24 prefetch.2.10.7: 1) Downloading 'SRR11978547'... 2020-07-14T01:48:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:28 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:28 prefetch.2.10.7: 'SRR11978547' is valid 2020-07-14T01:49:28 prefetch.2.10.7: 1) 'SRR11978547' was downloaded successfully 2020-07-14T01:49:28 prefetch.2.10.7: 'SRR11978547' has 0 unresolved dependencies Read 5480468 spots for SRR11978547/SRR11978547.sra Written 5480468 spots for SRR11978547/SRR11978547.sra 2020-07-14T01:50:02 prefetch.2.10.7: 1) Downloading 'SRR11978548'... 2020-07-14T01:50:02 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:50:27 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:50:27 prefetch.2.10.7: 'SRR11978548' is valid 2020-07-14T01:50:27 prefetch.2.10.7: 1) 'SRR11978548' was downloaded successfully 2020-07-14T01:50:27 prefetch.2.10.7: 'SRR11978548' has 0 unresolved dependencies Read 2237437 spots for SRR11978548/SRR11978548.sra Written 2237437 spots for SRR11978548/SRR11978548.sra 2020-07-14T01:50:49 prefetch.2.10.7: 1) Downloading 'SRR11978549'... 2020-07-14T01:50:49 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:51:12 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:51:12 prefetch.2.10.7: 'SRR11978549' is valid 2020-07-14T01:51:12 prefetch.2.10.7: 1) 'SRR11978549' was downloaded successfully 2020-07-14T01:51:12 prefetch.2.10.7: 'SRR11978549' has 0 unresolved dependencies Read 2216363 spots for SRR11978549/SRR11978549.sra Written 2216363 spots for SRR11978549/SRR11978549.sra 2020-07-14T01:51:31 prefetch.2.10.7: 1) Downloading 'SRR11978550'... 2020-07-14T01:51:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:51:55 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:51:55 prefetch.2.10.7: 'SRR11978550' is valid 2020-07-14T01:51:55 prefetch.2.10.7: 1) 'SRR11978550' was downloaded successfully 2020-07-14T01:51:55 prefetch.2.10.7: 'SRR11978550' has 0 unresolved dependencies Read 2248193 spots for SRR11978550/SRR11978550.sra Written 2248193 spots for SRR11978550/SRR11978550.sra 2020-07-14T01:52:17 prefetch.2.10.7: 1) Downloading 'SRR11978551'... 2020-07-14T01:52:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:52:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:52:39 prefetch.2.10.7: 'SRR11978551' is valid 2020-07-14T01:52:39 prefetch.2.10.7: 1) 'SRR11978551' was downloaded successfully 2020-07-14T01:52:39 prefetch.2.10.7: 'SRR11978551' has 0 unresolved dependencies Read 2221267 spots for SRR11978551/SRR11978551.sra Written 2221267 spots for SRR11978551/SRR11978551.sra 2020-07-14T01:53:01 prefetch.2.10.7: 1) Downloading 'SRR11978552'... 2020-07-14T01:53:01 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:53:25 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:53:25 prefetch.2.10.7: 'SRR11978552' is valid 2020-07-14T01:53:25 prefetch.2.10.7: 1) 'SRR11978552' was downloaded successfully 2020-07-14T01:53:25 prefetch.2.10.7: 'SRR11978552' has 0 unresolved dependencies Read 774782 spots for SRR11978552/SRR11978552.sra Written 774782 spots for SRR11978552/SRR11978552.sra 2020-07-14T01:53:42 prefetch.2.10.7: 1) Downloading 'SRR11978553'... 2020-07-14T01:53:42 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:54:03 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:54:03 prefetch.2.10.7: 'SRR11978553' is valid 2020-07-14T01:54:03 prefetch.2.10.7: 1) 'SRR11978553' was downloaded successfully 2020-07-14T01:54:03 prefetch.2.10.7: 'SRR11978553' has 0 unresolved dependencies Read 821852 spots for SRR11978553/SRR11978553.sra Written 821852 spots for SRR11978553/SRR11978553.sra 2020-07-14T01:54:21 prefetch.2.10.7: 1) Downloading 'SRR11978554'... 2020-07-14T01:54:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:54:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:54:41 prefetch.2.10.7: 'SRR11978554' is valid 2020-07-14T01:54:41 prefetch.2.10.7: 1) 'SRR11978554' was downloaded successfully 2020-07-14T01:54:41 prefetch.2.10.7: 'SRR11978554' has 0 unresolved dependencies Read 819460 spots for SRR11978554/SRR11978554.sra Written 819460 spots for SRR11978554/SRR11978554.sra 2020-07-14T01:54:58 prefetch.2.10.7: 1) Downloading 'SRR11978555'... 2020-07-14T01:54:58 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:28 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:55:29 prefetch.2.10.7: 'SRR11978555' is valid 2020-07-14T01:55:29 prefetch.2.10.7: 1) 'SRR11978555' was downloaded successfully 2020-07-14T01:55:29 prefetch.2.10.7: 'SRR11978555' has 0 unresolved dependencies Read 2148089 spots for SRR11978555/SRR11978555.sra Written 2148089 spots for SRR11978555/SRR11978555.sra 2020-07-14T01:55:46 prefetch.2.10.7: 1) Downloading 'SRR11978556'... 2020-07-14T01:55:46 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:09 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:09 prefetch.2.10.7: 'SRR11978556' is valid 2020-07-14T01:56:09 prefetch.2.10.7: 1) 'SRR11978556' was downloaded successfully 2020-07-14T01:56:09 prefetch.2.10.7: 'SRR11978556' has 0 unresolved dependencies Read 2034439 spots for SRR11978556/SRR11978556.sra Written 2034439 spots for SRR11978556/SRR11978556.sra 2020-07-14T01:56:30 prefetch.2.10.7: 1) Downloading 'SRR11978557'... 2020-07-14T01:56:30 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:58 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:58 prefetch.2.10.7: 'SRR11978557' is valid 2020-07-14T01:56:58 prefetch.2.10.7: 1) 'SRR11978557' was downloaded successfully 2020-07-14T01:56:58 prefetch.2.10.7: 'SRR11978557' has 0 unresolved dependencies Read 2160917 spots for SRR11978557/SRR11978557.sra Written 2160917 spots for SRR11978557/SRR11978557.sra 2020-07-14T01:57:20 prefetch.2.10.7: 1) Downloading 'SRR11978558'... 2020-07-14T01:57:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:57:56 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:57:56 prefetch.2.10.7: 'SRR11978558' is valid 2020-07-14T01:57:56 prefetch.2.10.7: 1) 'SRR11978558' was downloaded successfully 2020-07-14T01:57:56 prefetch.2.10.7: 'SRR11978558' has 0 unresolved dependencies Read 2142541 spots for SRR11978558/SRR11978558.sra Written 2142541 spots for SRR11978558/SRR11978558.sra 2020-07-14T01:58:17 prefetch.2.10.7: 1) Downloading 'SRR11978559'... 2020-07-14T01:58:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:59:11 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:59:12 prefetch.2.10.7: 'SRR11978559' is valid 2020-07-14T01:59:12 prefetch.2.10.7: 1) 'SRR11978559' was downloaded successfully 2020-07-14T01:59:12 prefetch.2.10.7: 'SRR11978559' has 0 unresolved dependencies Read 5520155 spots for SRR11978559/SRR11978559.sra Written 5520155 spots for SRR11978559/SRR11978559.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627515 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:46 42501714 reads; of these: 42501714 (100.00%) were unpaired; of these: 33915571 (79.80%) aligned 0 times 6580270 (15.48%) aligned exactly 1 time 2005873 (4.72%) aligned >1 times 20.20% overall alignment rate Time searching: 00:06:46 Overall time: 00:06:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1499750 / 8586143 = 0.1747 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:09:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:09:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:09:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:09:34: 1000000 INFO @ Tue, 14 Jul 2020 11:09:40: 2000000 INFO @ Tue, 14 Jul 2020 11:09:45: 3000000 INFO @ Tue, 14 Jul 2020 11:09:50: 4000000 INFO @ Tue, 14 Jul 2020 11:09:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:09:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:09:59: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:09:59: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:10:01: 6000000 INFO @ Tue, 14 Jul 2020 11:10:04: 1000000 INFO @ Tue, 14 Jul 2020 11:10:06: 7000000 INFO @ Tue, 14 Jul 2020 11:10:07: #1 tag size is determined as 63 bps INFO @ Tue, 14 Jul 2020 11:10:07: #1 tag size = 63 INFO @ Tue, 14 Jul 2020 11:10:07: #1 total tags in treatment: 7086393 INFO @ Tue, 14 Jul 2020 11:10:07: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:10:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:10:07: #1 tags after filtering in treatment: 7086393 INFO @ Tue, 14 Jul 2020 11:10:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:10:07: #1 finished! INFO @ Tue, 14 Jul 2020 11:10:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:10:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:10:08: #2 number of paired peaks: 252 WARNING @ Tue, 14 Jul 2020 11:10:08: Fewer paired peaks (252) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 252 pairs to build model! INFO @ Tue, 14 Jul 2020 11:10:08: start model_add_line... INFO @ Tue, 14 Jul 2020 11:10:08: start X-correlation... INFO @ Tue, 14 Jul 2020 11:10:08: end of X-cor INFO @ Tue, 14 Jul 2020 11:10:08: #2 finished! INFO @ Tue, 14 Jul 2020 11:10:08: #2 predicted fragment length is 55 bps INFO @ Tue, 14 Jul 2020 11:10:08: #2 alternative fragment length(s) may be 55 bps INFO @ Tue, 14 Jul 2020 11:10:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.05_model.r WARNING @ Tue, 14 Jul 2020 11:10:08: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:10:08: #2 You may need to consider one of the other alternative d(s): 55 WARNING @ Tue, 14 Jul 2020 11:10:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:10:08: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:10:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:10:10: 2000000 INFO @ Tue, 14 Jul 2020 11:10:15: 3000000 INFO @ Tue, 14 Jul 2020 11:10:21: 4000000 INFO @ Tue, 14 Jul 2020 11:10:23: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:10:26: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:10:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:10:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:10:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:10:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:10:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:10:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.05_summits.bed INFO @ Tue, 14 Jul 2020 11:10:31: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1764 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:10:31: 6000000 INFO @ Tue, 14 Jul 2020 11:10:34: 1000000 INFO @ Tue, 14 Jul 2020 11:10:37: 7000000 INFO @ Tue, 14 Jul 2020 11:10:37: #1 tag size is determined as 63 bps INFO @ Tue, 14 Jul 2020 11:10:37: #1 tag size = 63 INFO @ Tue, 14 Jul 2020 11:10:37: #1 total tags in treatment: 7086393 INFO @ Tue, 14 Jul 2020 11:10:37: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:10:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:10:38: #1 tags after filtering in treatment: 7086393 INFO @ Tue, 14 Jul 2020 11:10:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:10:38: #1 finished! INFO @ Tue, 14 Jul 2020 11:10:38: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:10:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:10:38: #2 number of paired peaks: 252 WARNING @ Tue, 14 Jul 2020 11:10:38: Fewer paired peaks (252) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 252 pairs to build model! INFO @ Tue, 14 Jul 2020 11:10:38: start model_add_line... INFO @ Tue, 14 Jul 2020 11:10:38: start X-correlation... INFO @ Tue, 14 Jul 2020 11:10:38: end of X-cor INFO @ Tue, 14 Jul 2020 11:10:38: #2 finished! INFO @ Tue, 14 Jul 2020 11:10:38: #2 predicted fragment length is 55 bps INFO @ Tue, 14 Jul 2020 11:10:38: #2 alternative fragment length(s) may be 55 bps INFO @ Tue, 14 Jul 2020 11:10:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.10_model.r WARNING @ Tue, 14 Jul 2020 11:10:38: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:10:38: #2 You may need to consider one of the other alternative d(s): 55 WARNING @ Tue, 14 Jul 2020 11:10:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:10:38: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:10:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:10:40: 2000000 INFO @ Tue, 14 Jul 2020 11:10:45: 3000000 INFO @ Tue, 14 Jul 2020 11:10:51: 4000000 INFO @ Tue, 14 Jul 2020 11:10:54: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:10:56: 5000000 INFO @ Tue, 14 Jul 2020 11:11:02: 6000000 INFO @ Tue, 14 Jul 2020 11:11:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:11:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:11:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.10_summits.bed INFO @ Tue, 14 Jul 2020 11:11:02: Done! pass1 - making usageList (13 chroms): 0 millis pass2 - checking and writing primary data (692 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:11:08: 7000000 INFO @ Tue, 14 Jul 2020 11:11:09: #1 tag size is determined as 63 bps INFO @ Tue, 14 Jul 2020 11:11:09: #1 tag size = 63 INFO @ Tue, 14 Jul 2020 11:11:09: #1 total tags in treatment: 7086393 INFO @ Tue, 14 Jul 2020 11:11:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:11:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:11:09: #1 tags after filtering in treatment: 7086393 INFO @ Tue, 14 Jul 2020 11:11:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:11:09: #1 finished! INFO @ Tue, 14 Jul 2020 11:11:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:11:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:11:09: #2 number of paired peaks: 252 WARNING @ Tue, 14 Jul 2020 11:11:09: Fewer paired peaks (252) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 252 pairs to build model! INFO @ Tue, 14 Jul 2020 11:11:09: start model_add_line... INFO @ Tue, 14 Jul 2020 11:11:09: start X-correlation... INFO @ Tue, 14 Jul 2020 11:11:09: end of X-cor INFO @ Tue, 14 Jul 2020 11:11:09: #2 finished! INFO @ Tue, 14 Jul 2020 11:11:09: #2 predicted fragment length is 55 bps INFO @ Tue, 14 Jul 2020 11:11:09: #2 alternative fragment length(s) may be 55 bps INFO @ Tue, 14 Jul 2020 11:11:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.20_model.r WARNING @ Tue, 14 Jul 2020 11:11:09: #2 Since the d (55) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:11:09: #2 You may need to consider one of the other alternative d(s): 55 WARNING @ Tue, 14 Jul 2020 11:11:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:11:09: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:11:09: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:11:24: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:11:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:11:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:11:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521337/SRX8521337.20_summits.bed INFO @ Tue, 14 Jul 2020 11:11:32: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (126 records, 4 fields): 2 millis CompletedMACS2peakCalling