Job ID = 6627262 SRX = SRX8521333 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:42:20 prefetch.2.10.7: 1) Downloading 'SRR11978013'... 2020-07-14T01:42:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:43:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:43:15 prefetch.2.10.7: 'SRR11978013' is valid 2020-07-14T01:43:15 prefetch.2.10.7: 1) 'SRR11978013' was downloaded successfully 2020-07-14T01:43:15 prefetch.2.10.7: 'SRR11978013' has 0 unresolved dependencies Read 3761869 spots for SRR11978013/SRR11978013.sra Written 3761869 spots for SRR11978013/SRR11978013.sra 2020-07-14T01:43:38 prefetch.2.10.7: 1) Downloading 'SRR11978014'... 2020-07-14T01:43:38 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:44:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:44:10 prefetch.2.10.7: 'SRR11978014' is valid 2020-07-14T01:44:10 prefetch.2.10.7: 1) 'SRR11978014' was downloaded successfully 2020-07-14T01:44:10 prefetch.2.10.7: 'SRR11978014' has 0 unresolved dependencies Read 3241027 spots for SRR11978014/SRR11978014.sra Written 3241027 spots for SRR11978014/SRR11978014.sra 2020-07-14T01:44:36 prefetch.2.10.7: 1) Downloading 'SRR11978015'... 2020-07-14T01:44:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:42 prefetch.2.10.7: 'SRR11978015' is valid 2020-07-14T01:47:42 prefetch.2.10.7: 1) 'SRR11978015' was downloaded successfully 2020-07-14T01:47:42 prefetch.2.10.7: 'SRR11978015' has 0 unresolved dependencies Read 3455890 spots for SRR11978015/SRR11978015.sra Written 3455890 spots for SRR11978015/SRR11978015.sra 2020-07-14T01:48:07 prefetch.2.10.7: 1) Downloading 'SRR11978016'... 2020-07-14T01:48:07 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:28 prefetch.2.10.7: HTTPS download failed 2020-07-14T01:55:28 prefetch.2.10.7: 1) failed to download SRR11978016 2020-07-14T01:55:38 prefetch.2.10.7: 1) Downloading 'SRR11978016'... 2020-07-14T01:55:38 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:55:38 prefetch.2.10.7: Continue download of 'SRR11978016' from 104584543 2020-07-14T01:55:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:55:50 prefetch.2.10.7: 'SRR11978016' is valid 2020-07-14T01:55:50 prefetch.2.10.7: 1) 'SRR11978016' was downloaded successfully 2020-07-14T01:55:50 prefetch.2.10.7: 'SRR11978016' has 0 unresolved dependencies Read 3387293 spots for SRR11978016/SRR11978016.sra Written 3387293 spots for SRR11978016/SRR11978016.sra 2020-07-14T01:56:21 prefetch.2.10.7: 1) Downloading 'SRR11978017'... 2020-07-14T01:56:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:56 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:57 prefetch.2.10.7: 'SRR11978017' is valid 2020-07-14T01:56:57 prefetch.2.10.7: 1) 'SRR11978017' was downloaded successfully 2020-07-14T01:56:57 prefetch.2.10.7: 'SRR11978017' has 0 unresolved dependencies Read 2688166 spots for SRR11978017/SRR11978017.sra Written 2688166 spots for SRR11978017/SRR11978017.sra 2020-07-14T01:57:20 prefetch.2.10.7: 1) Downloading 'SRR11978018'... 2020-07-14T01:57:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:58:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:58:07 prefetch.2.10.7: 'SRR11978018' is valid 2020-07-14T01:58:07 prefetch.2.10.7: 1) 'SRR11978018' was downloaded successfully 2020-07-14T01:58:07 prefetch.2.10.7: 'SRR11978018' has 0 unresolved dependencies Read 2757952 spots for SRR11978018/SRR11978018.sra Written 2757952 spots for SRR11978018/SRR11978018.sra 2020-07-14T01:58:27 prefetch.2.10.7: 1) Downloading 'SRR11978019'... 2020-07-14T01:58:27 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:59:01 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:59:01 prefetch.2.10.7: 'SRR11978019' is valid 2020-07-14T01:59:01 prefetch.2.10.7: 1) 'SRR11978019' was downloaded successfully 2020-07-14T01:59:01 prefetch.2.10.7: 'SRR11978019' has 0 unresolved dependencies Read 2763886 spots for SRR11978019/SRR11978019.sra Written 2763886 spots for SRR11978019/SRR11978019.sra 2020-07-14T01:59:24 prefetch.2.10.7: 1) Downloading 'SRR11978020'... 2020-07-14T01:59:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:00:08 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:00:08 prefetch.2.10.7: 'SRR11978020' is valid 2020-07-14T02:00:08 prefetch.2.10.7: 1) 'SRR11978020' was downloaded successfully 2020-07-14T02:00:08 prefetch.2.10.7: 'SRR11978020' has 0 unresolved dependencies Read 2697870 spots for SRR11978020/SRR11978020.sra Written 2697870 spots for SRR11978020/SRR11978020.sra 2020-07-14T02:00:28 prefetch.2.10.7: 1) Downloading 'SRR11978021'... 2020-07-14T02:00:28 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:01:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:01:15 prefetch.2.10.7: 'SRR11978021' is valid 2020-07-14T02:01:15 prefetch.2.10.7: 1) 'SRR11978021' was downloaded successfully 2020-07-14T02:01:15 prefetch.2.10.7: 'SRR11978021' has 0 unresolved dependencies Read 3589318 spots for SRR11978021/SRR11978021.sra Written 3589318 spots for SRR11978021/SRR11978021.sra 2020-07-14T02:01:40 prefetch.2.10.7: 1) Downloading 'SRR11978022'... 2020-07-14T02:01:40 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:03:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:03:39 prefetch.2.10.7: 'SRR11978022' is valid 2020-07-14T02:03:39 prefetch.2.10.7: 1) 'SRR11978022' was downloaded successfully 2020-07-14T02:03:39 prefetch.2.10.7: 'SRR11978022' has 0 unresolved dependencies Read 3940185 spots for SRR11978022/SRR11978022.sra Written 3940185 spots for SRR11978022/SRR11978022.sra 2020-07-14T02:04:03 prefetch.2.10.7: 1) Downloading 'SRR11978023'... 2020-07-14T02:04:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:05:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:05:54 prefetch.2.10.7: 'SRR11978023' is valid 2020-07-14T02:05:54 prefetch.2.10.7: 1) 'SRR11978023' was downloaded successfully 2020-07-14T02:05:54 prefetch.2.10.7: 'SRR11978023' has 0 unresolved dependencies Read 3944243 spots for SRR11978023/SRR11978023.sra Written 3944243 spots for SRR11978023/SRR11978023.sra 2020-07-14T02:06:21 prefetch.2.10.7: 1) Downloading 'SRR11978024'... 2020-07-14T02:06:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:06:45 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:06:45 prefetch.2.10.7: 'SRR11978024' is valid 2020-07-14T02:06:45 prefetch.2.10.7: 1) 'SRR11978024' was downloaded successfully 2020-07-14T02:06:45 prefetch.2.10.7: 'SRR11978024' has 0 unresolved dependencies Read 1108594 spots for SRR11978024/SRR11978024.sra Written 1108594 spots for SRR11978024/SRR11978024.sra 2020-07-14T02:06:59 prefetch.2.10.7: 1) Downloading 'SRR11978025'... 2020-07-14T02:06:59 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:07:17 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:07:17 prefetch.2.10.7: 'SRR11978025' is valid 2020-07-14T02:07:17 prefetch.2.10.7: 1) 'SRR11978025' was downloaded successfully 2020-07-14T02:07:17 prefetch.2.10.7: 'SRR11978025' has 0 unresolved dependencies Read 1077001 spots for SRR11978025/SRR11978025.sra Written 1077001 spots for SRR11978025/SRR11978025.sra 2020-07-14T02:07:35 prefetch.2.10.7: 1) Downloading 'SRR11978026'... 2020-07-14T02:07:35 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:08:04 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:08:04 prefetch.2.10.7: 'SRR11978026' is valid 2020-07-14T02:08:04 prefetch.2.10.7: 1) 'SRR11978026' was downloaded successfully 2020-07-14T02:08:04 prefetch.2.10.7: 'SRR11978026' has 0 unresolved dependencies Read 1128598 spots for SRR11978026/SRR11978026.sra Written 1128598 spots for SRR11978026/SRR11978026.sra 2020-07-14T02:08:19 prefetch.2.10.7: 1) Downloading 'SRR11978027'... 2020-07-14T02:08:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:08:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:08:41 prefetch.2.10.7: 'SRR11978027' is valid 2020-07-14T02:08:41 prefetch.2.10.7: 1) 'SRR11978027' was downloaded successfully 2020-07-14T02:08:41 prefetch.2.10.7: 'SRR11978027' has 0 unresolved dependencies Read 1139181 spots for SRR11978027/SRR11978027.sra Written 1139181 spots for SRR11978027/SRR11978027.sra 2020-07-14T02:09:00 prefetch.2.10.7: 1) Downloading 'SRR11978028'... 2020-07-14T02:09:00 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:09:35 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:09:36 prefetch.2.10.7: 'SRR11978028' is valid 2020-07-14T02:09:36 prefetch.2.10.7: 1) 'SRR11978028' was downloaded successfully 2020-07-14T02:09:36 prefetch.2.10.7: 'SRR11978028' has 0 unresolved dependencies Read 3346758 spots for SRR11978028/SRR11978028.sra Written 3346758 spots for SRR11978028/SRR11978028.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627550 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:06:23 44027831 reads; of these: 44027831 (100.00%) were unpaired; of these: 37695865 (85.62%) aligned 0 times 4782724 (10.86%) aligned exactly 1 time 1549242 (3.52%) aligned >1 times 14.38% overall alignment rate Time searching: 00:06:24 Overall time: 00:06:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1194152 / 6331966 = 0.1886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:18:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:18:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:18:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:18:55: 1000000 INFO @ Tue, 14 Jul 2020 11:19:01: 2000000 INFO @ Tue, 14 Jul 2020 11:19:07: 3000000 INFO @ Tue, 14 Jul 2020 11:19:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:19:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:19:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:19:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:19:20: 5000000 INFO @ Tue, 14 Jul 2020 11:19:21: #1 tag size is determined as 67 bps INFO @ Tue, 14 Jul 2020 11:19:21: #1 tag size = 67 INFO @ Tue, 14 Jul 2020 11:19:21: #1 total tags in treatment: 5137814 INFO @ Tue, 14 Jul 2020 11:19:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:19:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:19:21: #1 tags after filtering in treatment: 5137814 INFO @ Tue, 14 Jul 2020 11:19:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:19:21: #1 finished! INFO @ Tue, 14 Jul 2020 11:19:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:19:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:19:21: #2 number of paired peaks: 529 WARNING @ Tue, 14 Jul 2020 11:19:21: Fewer paired peaks (529) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 529 pairs to build model! INFO @ Tue, 14 Jul 2020 11:19:21: start model_add_line... INFO @ Tue, 14 Jul 2020 11:19:21: start X-correlation... INFO @ Tue, 14 Jul 2020 11:19:21: end of X-cor INFO @ Tue, 14 Jul 2020 11:19:21: #2 finished! INFO @ Tue, 14 Jul 2020 11:19:21: #2 predicted fragment length is 74 bps INFO @ Tue, 14 Jul 2020 11:19:21: #2 alternative fragment length(s) may be 74 bps INFO @ Tue, 14 Jul 2020 11:19:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.05_model.r WARNING @ Tue, 14 Jul 2020 11:19:21: #2 Since the d (74) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:19:21: #2 You may need to consider one of the other alternative d(s): 74 WARNING @ Tue, 14 Jul 2020 11:19:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:19:21: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:19:21: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:19:26: 1000000 INFO @ Tue, 14 Jul 2020 11:19:32: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:19:33: 2000000 INFO @ Tue, 14 Jul 2020 11:19:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:19:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:19:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.05_summits.bed INFO @ Tue, 14 Jul 2020 11:19:37: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2002 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:19:39: 3000000 INFO @ Tue, 14 Jul 2020 11:19:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:19:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:19:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:19:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:19:53: 5000000 INFO @ Tue, 14 Jul 2020 11:19:54: #1 tag size is determined as 67 bps INFO @ Tue, 14 Jul 2020 11:19:54: #1 tag size = 67 INFO @ Tue, 14 Jul 2020 11:19:54: #1 total tags in treatment: 5137814 INFO @ Tue, 14 Jul 2020 11:19:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:19:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:19:54: #1 tags after filtering in treatment: 5137814 INFO @ Tue, 14 Jul 2020 11:19:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:19:54: #1 finished! INFO @ Tue, 14 Jul 2020 11:19:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:19:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:19:54: #2 number of paired peaks: 529 WARNING @ Tue, 14 Jul 2020 11:19:54: Fewer paired peaks (529) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 529 pairs to build model! INFO @ Tue, 14 Jul 2020 11:19:54: start model_add_line... INFO @ Tue, 14 Jul 2020 11:19:54: start X-correlation... INFO @ Tue, 14 Jul 2020 11:19:54: end of X-cor INFO @ Tue, 14 Jul 2020 11:19:54: #2 finished! INFO @ Tue, 14 Jul 2020 11:19:54: #2 predicted fragment length is 74 bps INFO @ Tue, 14 Jul 2020 11:19:54: #2 alternative fragment length(s) may be 74 bps INFO @ Tue, 14 Jul 2020 11:19:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.10_model.r WARNING @ Tue, 14 Jul 2020 11:19:54: #2 Since the d (74) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:19:54: #2 You may need to consider one of the other alternative d(s): 74 WARNING @ Tue, 14 Jul 2020 11:19:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:19:54: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:19:54: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:19:58: 1000000 INFO @ Tue, 14 Jul 2020 11:20:05: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:20:06: 2000000 INFO @ Tue, 14 Jul 2020 11:20:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:20:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:20:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.10_summits.bed INFO @ Tue, 14 Jul 2020 11:20:11: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (824 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:20:14: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:20:22: 4000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:20:30: 5000000 INFO @ Tue, 14 Jul 2020 11:20:31: #1 tag size is determined as 67 bps INFO @ Tue, 14 Jul 2020 11:20:31: #1 tag size = 67 INFO @ Tue, 14 Jul 2020 11:20:31: #1 total tags in treatment: 5137814 INFO @ Tue, 14 Jul 2020 11:20:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:20:31: #1 tags after filtering in treatment: 5137814 INFO @ Tue, 14 Jul 2020 11:20:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:20:31: #1 finished! INFO @ Tue, 14 Jul 2020 11:20:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:20:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:20:31: #2 number of paired peaks: 529 WARNING @ Tue, 14 Jul 2020 11:20:31: Fewer paired peaks (529) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 529 pairs to build model! INFO @ Tue, 14 Jul 2020 11:20:31: start model_add_line... INFO @ Tue, 14 Jul 2020 11:20:31: start X-correlation... INFO @ Tue, 14 Jul 2020 11:20:31: end of X-cor INFO @ Tue, 14 Jul 2020 11:20:31: #2 finished! INFO @ Tue, 14 Jul 2020 11:20:31: #2 predicted fragment length is 74 bps INFO @ Tue, 14 Jul 2020 11:20:31: #2 alternative fragment length(s) may be 74 bps INFO @ Tue, 14 Jul 2020 11:20:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.20_model.r WARNING @ Tue, 14 Jul 2020 11:20:31: #2 Since the d (74) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:20:31: #2 You may need to consider one of the other alternative d(s): 74 WARNING @ Tue, 14 Jul 2020 11:20:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:20:31: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:20:31: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:20:42: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:20:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:20:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:20:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521333/SRX8521333.20_summits.bed INFO @ Tue, 14 Jul 2020 11:20:48: Done! pass1 - making usageList (11 chroms): 0 millis pass2 - checking and writing primary data (205 records, 4 fields): 2 millis CompletedMACS2peakCalling