Job ID = 6627261 SRX = SRX8521332 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:42:20 prefetch.2.10.7: 1) Downloading 'SRR11977997'... 2020-07-14T01:42:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:43:01 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:43:02 prefetch.2.10.7: 'SRR11977997' is valid 2020-07-14T01:43:02 prefetch.2.10.7: 1) 'SRR11977997' was downloaded successfully 2020-07-14T01:43:02 prefetch.2.10.7: 'SRR11977997' has 0 unresolved dependencies Read 4449536 spots for SRR11977997/SRR11977997.sra Written 4449536 spots for SRR11977997/SRR11977997.sra 2020-07-14T01:43:34 prefetch.2.10.7: 1) Downloading 'SRR11977998'... 2020-07-14T01:43:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:44:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:44:14 prefetch.2.10.7: 'SRR11977998' is valid 2020-07-14T01:44:14 prefetch.2.10.7: 1) 'SRR11977998' was downloaded successfully 2020-07-14T01:44:14 prefetch.2.10.7: 'SRR11977998' has 0 unresolved dependencies Read 2574073 spots for SRR11977998/SRR11977998.sra Written 2574073 spots for SRR11977998/SRR11977998.sra 2020-07-14T01:44:36 prefetch.2.10.7: 1) Downloading 'SRR11977999'... 2020-07-14T01:44:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:03 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:04 prefetch.2.10.7: 'SRR11977999' is valid 2020-07-14T01:45:04 prefetch.2.10.7: 1) 'SRR11977999' was downloaded successfully 2020-07-14T01:45:04 prefetch.2.10.7: 'SRR11977999' has 0 unresolved dependencies Read 2757963 spots for SRR11977999/SRR11977999.sra Written 2757963 spots for SRR11977999/SRR11977999.sra 2020-07-14T01:45:24 prefetch.2.10.7: 1) Downloading 'SRR11978000'... 2020-07-14T01:45:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:46:04 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:46:04 prefetch.2.10.7: 'SRR11978000' is valid 2020-07-14T01:46:04 prefetch.2.10.7: 1) 'SRR11978000' was downloaded successfully 2020-07-14T01:46:04 prefetch.2.10.7: 'SRR11978000' has 0 unresolved dependencies Read 2733460 spots for SRR11978000/SRR11978000.sra Written 2733460 spots for SRR11978000/SRR11978000.sra 2020-07-14T01:46:24 prefetch.2.10.7: 1) Downloading 'SRR11978001'... 2020-07-14T01:46:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:46:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:00 prefetch.2.10.7: 'SRR11978001' is valid 2020-07-14T01:47:00 prefetch.2.10.7: 1) 'SRR11978001' was downloaded successfully 2020-07-14T01:47:00 prefetch.2.10.7: 'SRR11978001' has 0 unresolved dependencies Read 4346542 spots for SRR11978001/SRR11978001.sra Written 4346542 spots for SRR11978001/SRR11978001.sra 2020-07-14T01:47:31 prefetch.2.10.7: 1) Downloading 'SRR11978002'... 2020-07-14T01:47:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:48:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:48:13 prefetch.2.10.7: 'SRR11978002' is valid 2020-07-14T01:48:13 prefetch.2.10.7: 1) 'SRR11978002' was downloaded successfully 2020-07-14T01:48:13 prefetch.2.10.7: 'SRR11978002' has 0 unresolved dependencies Read 4477516 spots for SRR11978002/SRR11978002.sra Written 4477516 spots for SRR11978002/SRR11978002.sra 2020-07-14T01:48:43 prefetch.2.10.7: 1) Downloading 'SRR11978003'... 2020-07-14T01:48:43 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:50:40 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:50:40 prefetch.2.10.7: 'SRR11978003' is valid 2020-07-14T01:50:40 prefetch.2.10.7: 1) 'SRR11978003' was downloaded successfully 2020-07-14T01:50:40 prefetch.2.10.7: 'SRR11978003' has 0 unresolved dependencies Read 4452082 spots for SRR11978003/SRR11978003.sra Written 4452082 spots for SRR11978003/SRR11978003.sra 2020-07-14T01:51:09 prefetch.2.10.7: 1) Downloading 'SRR11978004'... 2020-07-14T01:51:09 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:52:17 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:52:17 prefetch.2.10.7: 'SRR11978004' is valid 2020-07-14T01:52:17 prefetch.2.10.7: 1) 'SRR11978004' was downloaded successfully 2020-07-14T01:52:17 prefetch.2.10.7: 'SRR11978004' has 0 unresolved dependencies Read 4284205 spots for SRR11978004/SRR11978004.sra Written 4284205 spots for SRR11978004/SRR11978004.sra 2020-07-14T01:52:49 prefetch.2.10.7: 1) Downloading 'SRR11978005'... 2020-07-14T01:52:49 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:54:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:54:50 prefetch.2.10.7: 'SRR11978005' is valid 2020-07-14T01:54:50 prefetch.2.10.7: 1) 'SRR11978005' was downloaded successfully 2020-07-14T01:54:50 prefetch.2.10.7: 'SRR11978005' has 0 unresolved dependencies Read 4089558 spots for SRR11978005/SRR11978005.sra Written 4089558 spots for SRR11978005/SRR11978005.sra 2020-07-14T01:55:14 prefetch.2.10.7: 1) Downloading 'SRR11978006'... 2020-07-14T01:55:14 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:56:05 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:56:05 prefetch.2.10.7: 'SRR11978006' is valid 2020-07-14T01:56:05 prefetch.2.10.7: 1) 'SRR11978006' was downloaded successfully 2020-07-14T01:56:05 prefetch.2.10.7: 'SRR11978006' has 0 unresolved dependencies Read 4478130 spots for SRR11978006/SRR11978006.sra Written 4478130 spots for SRR11978006/SRR11978006.sra 2020-07-14T01:56:35 prefetch.2.10.7: 1) Downloading 'SRR11978007'... 2020-07-14T01:56:35 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:58:07 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:58:08 prefetch.2.10.7: 'SRR11978007' is valid 2020-07-14T01:58:08 prefetch.2.10.7: 1) 'SRR11978007' was downloaded successfully 2020-07-14T01:58:08 prefetch.2.10.7: 'SRR11978007' has 0 unresolved dependencies Read 4623548 spots for SRR11978007/SRR11978007.sra Written 4623548 spots for SRR11978007/SRR11978007.sra 2020-07-14T01:58:38 prefetch.2.10.7: 1) Downloading 'SRR11978008'... 2020-07-14T01:58:38 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:59:00 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:59:00 prefetch.2.10.7: 'SRR11978008' is valid 2020-07-14T01:59:00 prefetch.2.10.7: 1) 'SRR11978008' was downloaded successfully 2020-07-14T01:59:00 prefetch.2.10.7: 'SRR11978008' has 0 unresolved dependencies Read 1062995 spots for SRR11978008/SRR11978008.sra Written 1062995 spots for SRR11978008/SRR11978008.sra 2020-07-14T01:59:17 prefetch.2.10.7: 1) Downloading 'SRR11978009'... 2020-07-14T01:59:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:59:42 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:59:42 prefetch.2.10.7: 'SRR11978009' is valid 2020-07-14T01:59:42 prefetch.2.10.7: 1) 'SRR11978009' was downloaded successfully 2020-07-14T01:59:42 prefetch.2.10.7: 'SRR11978009' has 0 unresolved dependencies Read 998630 spots for SRR11978009/SRR11978009.sra Written 998630 spots for SRR11978009/SRR11978009.sra 2020-07-14T01:59:59 prefetch.2.10.7: 1) Downloading 'SRR11978010'... 2020-07-14T01:59:59 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:00:26 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:00:26 prefetch.2.10.7: 'SRR11978010' is valid 2020-07-14T02:00:26 prefetch.2.10.7: 1) 'SRR11978010' was downloaded successfully 2020-07-14T02:00:26 prefetch.2.10.7: 'SRR11978010' has 0 unresolved dependencies Read 1027333 spots for SRR11978010/SRR11978010.sra Written 1027333 spots for SRR11978010/SRR11978010.sra 2020-07-14T02:00:51 prefetch.2.10.7: 1) Downloading 'SRR11978011'... 2020-07-14T02:00:51 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:01:12 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:01:12 prefetch.2.10.7: 'SRR11978011' is valid 2020-07-14T02:01:12 prefetch.2.10.7: 1) 'SRR11978011' was downloaded successfully 2020-07-14T02:01:12 prefetch.2.10.7: 'SRR11978011' has 0 unresolved dependencies Read 1052430 spots for SRR11978011/SRR11978011.sra Written 1052430 spots for SRR11978011/SRR11978011.sra 2020-07-14T02:01:33 prefetch.2.10.7: 1) Downloading 'SRR11978012'... 2020-07-14T02:01:33 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:02:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:02:13 prefetch.2.10.7: 'SRR11978012' is valid 2020-07-14T02:02:13 prefetch.2.10.7: 1) 'SRR11978012' was downloaded successfully 2020-07-14T02:02:13 prefetch.2.10.7: 'SRR11978012' has 0 unresolved dependencies Read 2750747 spots for SRR11978012/SRR11978012.sra Written 2750747 spots for SRR11978012/SRR11978012.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627522 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:29 50158748 reads; of these: 50158748 (100.00%) were unpaired; of these: 42764480 (85.26%) aligned 0 times 5612850 (11.19%) aligned exactly 1 time 1781418 (3.55%) aligned >1 times 14.74% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1700854 / 7394268 = 0.2300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:12:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:12:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:12:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:13:01: 1000000 INFO @ Tue, 14 Jul 2020 11:13:06: 2000000 INFO @ Tue, 14 Jul 2020 11:13:11: 3000000 INFO @ Tue, 14 Jul 2020 11:13:16: 4000000 INFO @ Tue, 14 Jul 2020 11:13:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:13:25: #1 tag size is determined as 66 bps INFO @ Tue, 14 Jul 2020 11:13:25: #1 tag size = 66 INFO @ Tue, 14 Jul 2020 11:13:25: #1 total tags in treatment: 5693414 INFO @ Tue, 14 Jul 2020 11:13:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:13:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:13:26: #1 tags after filtering in treatment: 5693414 INFO @ Tue, 14 Jul 2020 11:13:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:13:26: #1 finished! INFO @ Tue, 14 Jul 2020 11:13:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:13:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:13:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:13:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:13:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:13:26: #2 number of paired peaks: 490 WARNING @ Tue, 14 Jul 2020 11:13:26: Fewer paired peaks (490) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 490 pairs to build model! INFO @ Tue, 14 Jul 2020 11:13:26: start model_add_line... INFO @ Tue, 14 Jul 2020 11:13:26: start X-correlation... INFO @ Tue, 14 Jul 2020 11:13:26: end of X-cor INFO @ Tue, 14 Jul 2020 11:13:26: #2 finished! INFO @ Tue, 14 Jul 2020 11:13:26: #2 predicted fragment length is 68 bps INFO @ Tue, 14 Jul 2020 11:13:26: #2 alternative fragment length(s) may be 68 bps INFO @ Tue, 14 Jul 2020 11:13:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.05_model.r WARNING @ Tue, 14 Jul 2020 11:13:26: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:13:26: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Tue, 14 Jul 2020 11:13:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:13:26: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:13:26: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:13:32: 1000000 INFO @ Tue, 14 Jul 2020 11:13:38: 2000000 INFO @ Tue, 14 Jul 2020 11:13:38: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:13:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:13:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:13:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.05_summits.bed INFO @ Tue, 14 Jul 2020 11:13:44: Done! INFO @ Tue, 14 Jul 2020 11:13:44: 3000000 pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1994 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:13:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:13:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:13:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:13:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:13:57: 5000000 INFO @ Tue, 14 Jul 2020 11:14:01: #1 tag size is determined as 66 bps INFO @ Tue, 14 Jul 2020 11:14:01: #1 tag size = 66 INFO @ Tue, 14 Jul 2020 11:14:01: #1 total tags in treatment: 5693414 INFO @ Tue, 14 Jul 2020 11:14:01: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:14:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:14:01: #1 tags after filtering in treatment: 5693414 INFO @ Tue, 14 Jul 2020 11:14:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:14:01: #1 finished! INFO @ Tue, 14 Jul 2020 11:14:01: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:14:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:14:02: #2 number of paired peaks: 490 WARNING @ Tue, 14 Jul 2020 11:14:02: Fewer paired peaks (490) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 490 pairs to build model! INFO @ Tue, 14 Jul 2020 11:14:02: start model_add_line... INFO @ Tue, 14 Jul 2020 11:14:02: start X-correlation... INFO @ Tue, 14 Jul 2020 11:14:02: end of X-cor INFO @ Tue, 14 Jul 2020 11:14:02: #2 finished! INFO @ Tue, 14 Jul 2020 11:14:02: #2 predicted fragment length is 68 bps INFO @ Tue, 14 Jul 2020 11:14:02: #2 alternative fragment length(s) may be 68 bps INFO @ Tue, 14 Jul 2020 11:14:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.10_model.r WARNING @ Tue, 14 Jul 2020 11:14:02: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:14:02: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Tue, 14 Jul 2020 11:14:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:14:02: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:14:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:14:02: 1000000 INFO @ Tue, 14 Jul 2020 11:14:08: 2000000 INFO @ Tue, 14 Jul 2020 11:14:14: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:14:14: 3000000 INFO @ Tue, 14 Jul 2020 11:14:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:14:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:14:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.10_summits.bed INFO @ Tue, 14 Jul 2020 11:14:20: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (732 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:14:20: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:14:27: 5000000 INFO @ Tue, 14 Jul 2020 11:14:31: #1 tag size is determined as 66 bps INFO @ Tue, 14 Jul 2020 11:14:31: #1 tag size = 66 INFO @ Tue, 14 Jul 2020 11:14:31: #1 total tags in treatment: 5693414 INFO @ Tue, 14 Jul 2020 11:14:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:14:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:14:31: #1 tags after filtering in treatment: 5693414 INFO @ Tue, 14 Jul 2020 11:14:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:14:31: #1 finished! INFO @ Tue, 14 Jul 2020 11:14:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:14:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:14:31: #2 number of paired peaks: 490 WARNING @ Tue, 14 Jul 2020 11:14:31: Fewer paired peaks (490) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 490 pairs to build model! INFO @ Tue, 14 Jul 2020 11:14:31: start model_add_line... INFO @ Tue, 14 Jul 2020 11:14:32: start X-correlation... INFO @ Tue, 14 Jul 2020 11:14:32: end of X-cor INFO @ Tue, 14 Jul 2020 11:14:32: #2 finished! INFO @ Tue, 14 Jul 2020 11:14:32: #2 predicted fragment length is 68 bps INFO @ Tue, 14 Jul 2020 11:14:32: #2 alternative fragment length(s) may be 68 bps INFO @ Tue, 14 Jul 2020 11:14:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.20_model.r WARNING @ Tue, 14 Jul 2020 11:14:32: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:14:32: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Tue, 14 Jul 2020 11:14:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:14:32: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:14:32: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:14:43: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:14:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:14:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:14:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521332/SRX8521332.20_summits.bed INFO @ Tue, 14 Jul 2020 11:14:49: Done! pass1 - making usageList (12 chroms): 0 millis pass2 - checking and writing primary data (202 records, 4 fields): 2 millis CompletedMACS2peakCalling