Job ID = 6627245 SRX = SRX8521321 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:35:42 prefetch.2.10.7: 1) Downloading 'SRR11977941'... 2020-07-14T01:35:42 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:52 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:52 prefetch.2.10.7: 'SRR11977941' is valid 2020-07-14T01:35:52 prefetch.2.10.7: 1) 'SRR11977941' was downloaded successfully 2020-07-14T01:35:52 prefetch.2.10.7: 'SRR11977941' has 0 unresolved dependencies Read 57290 spots for SRR11977941/SRR11977941.sra Written 57290 spots for SRR11977941/SRR11977941.sra 2020-07-14T01:36:07 prefetch.2.10.7: 1) Downloading 'SRR11977942'... 2020-07-14T01:36:07 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:36:22 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:36:22 prefetch.2.10.7: 'SRR11977942' is valid 2020-07-14T01:36:22 prefetch.2.10.7: 1) 'SRR11977942' was downloaded successfully 2020-07-14T01:36:22 prefetch.2.10.7: 'SRR11977942' has 0 unresolved dependencies Read 93249 spots for SRR11977942/SRR11977942.sra Written 93249 spots for SRR11977942/SRR11977942.sra 2020-07-14T01:36:36 prefetch.2.10.7: 1) Downloading 'SRR11977943'... 2020-07-14T01:36:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:36:51 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:36:51 prefetch.2.10.7: 'SRR11977943' is valid 2020-07-14T01:36:51 prefetch.2.10.7: 1) 'SRR11977943' was downloaded successfully 2020-07-14T01:36:51 prefetch.2.10.7: 'SRR11977943' has 0 unresolved dependencies Read 99802 spots for SRR11977943/SRR11977943.sra Written 99802 spots for SRR11977943/SRR11977943.sra 2020-07-14T01:37:02 prefetch.2.10.7: 1) Downloading 'SRR11977944'... 2020-07-14T01:37:02 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:13 prefetch.2.10.7: 'SRR11977944' is valid 2020-07-14T01:37:13 prefetch.2.10.7: 1) 'SRR11977944' was downloaded successfully 2020-07-14T01:37:13 prefetch.2.10.7: 'SRR11977944' has 0 unresolved dependencies Read 98804 spots for SRR11977944/SRR11977944.sra Written 98804 spots for SRR11977944/SRR11977944.sra 2020-07-14T01:37:28 prefetch.2.10.7: 1) Downloading 'SRR11977945'... 2020-07-14T01:37:28 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:55 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:55 prefetch.2.10.7: 'SRR11977945' is valid 2020-07-14T01:37:55 prefetch.2.10.7: 1) 'SRR11977945' was downloaded successfully 2020-07-14T01:37:55 prefetch.2.10.7: 'SRR11977945' has 0 unresolved dependencies Read 2973015 spots for SRR11977945/SRR11977945.sra Written 2973015 spots for SRR11977945/SRR11977945.sra 2020-07-14T01:38:19 prefetch.2.10.7: 1) Downloading 'SRR11977946'... 2020-07-14T01:38:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:39:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:39:06 prefetch.2.10.7: 'SRR11977946' is valid 2020-07-14T01:39:06 prefetch.2.10.7: 1) 'SRR11977946' was downloaded successfully 2020-07-14T01:39:06 prefetch.2.10.7: 'SRR11977946' has 0 unresolved dependencies Read 2965262 spots for SRR11977946/SRR11977946.sra Written 2965262 spots for SRR11977946/SRR11977946.sra 2020-07-14T01:39:31 prefetch.2.10.7: 1) Downloading 'SRR11977947'... 2020-07-14T01:39:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:40:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:40:06 prefetch.2.10.7: 'SRR11977947' is valid 2020-07-14T01:40:06 prefetch.2.10.7: 1) 'SRR11977947' was downloaded successfully 2020-07-14T01:40:06 prefetch.2.10.7: 'SRR11977947' has 0 unresolved dependencies Read 3009484 spots for SRR11977947/SRR11977947.sra Written 3009484 spots for SRR11977947/SRR11977947.sra 2020-07-14T01:40:26 prefetch.2.10.7: 1) Downloading 'SRR11977948'... 2020-07-14T01:40:26 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:41:02 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:41:02 prefetch.2.10.7: 'SRR11977948' is valid 2020-07-14T01:41:02 prefetch.2.10.7: 1) 'SRR11977948' was downloaded successfully 2020-07-14T01:41:02 prefetch.2.10.7: 'SRR11977948' has 0 unresolved dependencies Read 2956746 spots for SRR11977948/SRR11977948.sra Written 2956746 spots for SRR11977948/SRR11977948.sra 2020-07-14T01:41:26 prefetch.2.10.7: 1) Downloading 'SRR11977949'... 2020-07-14T01:41:26 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:41:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:41:41 prefetch.2.10.7: 'SRR11977949' is valid 2020-07-14T01:41:41 prefetch.2.10.7: 1) 'SRR11977949' was downloaded successfully 2020-07-14T01:41:41 prefetch.2.10.7: 'SRR11977949' has 0 unresolved dependencies Read 54413 spots for SRR11977949/SRR11977949.sra Written 54413 spots for SRR11977949/SRR11977949.sra 2020-07-14T01:41:52 prefetch.2.10.7: 1) Downloading 'SRR11977950'... 2020-07-14T01:41:52 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:42:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:42:06 prefetch.2.10.7: 'SRR11977950' is valid 2020-07-14T01:42:06 prefetch.2.10.7: 1) 'SRR11977950' was downloaded successfully 2020-07-14T01:42:06 prefetch.2.10.7: 'SRR11977950' has 0 unresolved dependencies Read 57086 spots for SRR11977950/SRR11977950.sra Written 57086 spots for SRR11977950/SRR11977950.sra 2020-07-14T01:42:17 prefetch.2.10.7: 1) Downloading 'SRR11977951'... 2020-07-14T01:42:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:42:27 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:42:27 prefetch.2.10.7: 'SRR11977951' is valid 2020-07-14T01:42:27 prefetch.2.10.7: 1) 'SRR11977951' was downloaded successfully 2020-07-14T01:42:27 prefetch.2.10.7: 'SRR11977951' has 0 unresolved dependencies Read 56612 spots for SRR11977951/SRR11977951.sra Written 56612 spots for SRR11977951/SRR11977951.sra 2020-07-14T01:42:42 prefetch.2.10.7: 1) Downloading 'SRR11977952'... 2020-07-14T01:42:42 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:43:52 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:43:52 prefetch.2.10.7: 'SRR11977952' is valid 2020-07-14T01:43:52 prefetch.2.10.7: 1) 'SRR11977952' was downloaded successfully 2020-07-14T01:43:52 prefetch.2.10.7: 'SRR11977952' has 0 unresolved dependencies Read 9929469 spots for SRR11977952/SRR11977952.sra Written 9929469 spots for SRR11977952/SRR11977952.sra 2020-07-14T01:44:40 prefetch.2.10.7: 1) Downloading 'SRR11977953'... 2020-07-14T01:44:40 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:54 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:54 prefetch.2.10.7: 'SRR11977953' is valid 2020-07-14T01:45:54 prefetch.2.10.7: 1) 'SRR11977953' was downloaded successfully 2020-07-14T01:45:54 prefetch.2.10.7: 'SRR11977953' has 0 unresolved dependencies Read 9612274 spots for SRR11977953/SRR11977953.sra Written 9612274 spots for SRR11977953/SRR11977953.sra 2020-07-14T01:46:35 prefetch.2.10.7: 1) Downloading 'SRR11977954'... 2020-07-14T01:46:35 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:53 prefetch.2.10.7: 'SRR11977954' is valid 2020-07-14T01:47:53 prefetch.2.10.7: 1) 'SRR11977954' was downloaded successfully 2020-07-14T01:47:53 prefetch.2.10.7: 'SRR11977954' has 0 unresolved dependencies Read 9905795 spots for SRR11977954/SRR11977954.sra Written 9905795 spots for SRR11977954/SRR11977954.sra 2020-07-14T01:48:39 prefetch.2.10.7: 1) Downloading 'SRR11977955'... 2020-07-14T01:48:39 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:28 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:28 prefetch.2.10.7: 'SRR11977955' is valid 2020-07-14T01:49:28 prefetch.2.10.7: 1) 'SRR11977955' was downloaded successfully 2020-07-14T01:49:28 prefetch.2.10.7: 'SRR11977955' has 0 unresolved dependencies Read 9629044 spots for SRR11977955/SRR11977955.sra Written 9629044 spots for SRR11977955/SRR11977955.sra 2020-07-14T01:50:10 prefetch.2.10.7: 1) Downloading 'SRR11977956'... 2020-07-14T01:50:10 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:50:21 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:50:21 prefetch.2.10.7: 'SRR11977956' is valid 2020-07-14T01:50:21 prefetch.2.10.7: 1) 'SRR11977956' was downloaded successfully 2020-07-14T01:50:21 prefetch.2.10.7: 'SRR11977956' has 0 unresolved dependencies Read 98773 spots for SRR11977956/SRR11977956.sra Written 98773 spots for SRR11977956/SRR11977956.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627472 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:00 51597118 reads; of these: 51597118 (100.00%) were unpaired; of these: 41307122 (80.06%) aligned 0 times 8104106 (15.71%) aligned exactly 1 time 2185890 (4.24%) aligned >1 times 19.94% overall alignment rate Time searching: 00:07:00 Overall time: 00:07:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2241856 / 10289996 = 0.2179 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:00:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:00:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:00:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:00:48: 1000000 INFO @ Tue, 14 Jul 2020 11:00:54: 2000000 INFO @ Tue, 14 Jul 2020 11:01:00: 3000000 INFO @ Tue, 14 Jul 2020 11:01:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:01:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:01:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:01:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:01:13: 5000000 INFO @ Tue, 14 Jul 2020 11:01:18: 1000000 INFO @ Tue, 14 Jul 2020 11:01:20: 6000000 INFO @ Tue, 14 Jul 2020 11:01:25: 2000000 INFO @ Tue, 14 Jul 2020 11:01:27: 7000000 INFO @ Tue, 14 Jul 2020 11:01:31: 3000000 INFO @ Tue, 14 Jul 2020 11:01:33: 8000000 INFO @ Tue, 14 Jul 2020 11:01:34: #1 tag size is determined as 57 bps INFO @ Tue, 14 Jul 2020 11:01:34: #1 tag size = 57 INFO @ Tue, 14 Jul 2020 11:01:34: #1 total tags in treatment: 8048140 INFO @ Tue, 14 Jul 2020 11:01:34: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:01:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:01:34: #1 tags after filtering in treatment: 8048140 INFO @ Tue, 14 Jul 2020 11:01:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:01:34: #1 finished! INFO @ Tue, 14 Jul 2020 11:01:34: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:01:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:01:34: #2 number of paired peaks: 253 WARNING @ Tue, 14 Jul 2020 11:01:34: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Tue, 14 Jul 2020 11:01:34: start model_add_line... INFO @ Tue, 14 Jul 2020 11:01:34: start X-correlation... INFO @ Tue, 14 Jul 2020 11:01:34: end of X-cor INFO @ Tue, 14 Jul 2020 11:01:34: #2 finished! INFO @ Tue, 14 Jul 2020 11:01:34: #2 predicted fragment length is 42 bps INFO @ Tue, 14 Jul 2020 11:01:34: #2 alternative fragment length(s) may be 4,13,42,547,573 bps INFO @ Tue, 14 Jul 2020 11:01:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.05_model.r WARNING @ Tue, 14 Jul 2020 11:01:34: #2 Since the d (42) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:01:34: #2 You may need to consider one of the other alternative d(s): 4,13,42,547,573 WARNING @ Tue, 14 Jul 2020 11:01:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:01:34: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:01:34: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:01:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:01:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:01:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:01:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:01:44: 5000000 INFO @ Tue, 14 Jul 2020 11:01:48: 1000000 INFO @ Tue, 14 Jul 2020 11:01:50: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:01:51: 6000000 INFO @ Tue, 14 Jul 2020 11:01:55: 2000000 INFO @ Tue, 14 Jul 2020 11:01:58: 7000000 INFO @ Tue, 14 Jul 2020 11:01:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:01:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:01:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.05_summits.bed INFO @ Tue, 14 Jul 2020 11:01:58: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (1860 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:02:01: 3000000 INFO @ Tue, 14 Jul 2020 11:02:04: 8000000 INFO @ Tue, 14 Jul 2020 11:02:05: #1 tag size is determined as 57 bps INFO @ Tue, 14 Jul 2020 11:02:05: #1 tag size = 57 INFO @ Tue, 14 Jul 2020 11:02:05: #1 total tags in treatment: 8048140 INFO @ Tue, 14 Jul 2020 11:02:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:02:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:02:05: #1 tags after filtering in treatment: 8048140 INFO @ Tue, 14 Jul 2020 11:02:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:02:05: #1 finished! INFO @ Tue, 14 Jul 2020 11:02:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:02:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:02:05: #2 number of paired peaks: 253 WARNING @ Tue, 14 Jul 2020 11:02:05: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Tue, 14 Jul 2020 11:02:05: start model_add_line... INFO @ Tue, 14 Jul 2020 11:02:05: start X-correlation... INFO @ Tue, 14 Jul 2020 11:02:05: end of X-cor INFO @ Tue, 14 Jul 2020 11:02:05: #2 finished! INFO @ Tue, 14 Jul 2020 11:02:05: #2 predicted fragment length is 42 bps INFO @ Tue, 14 Jul 2020 11:02:05: #2 alternative fragment length(s) may be 4,13,42,547,573 bps INFO @ Tue, 14 Jul 2020 11:02:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.10_model.r WARNING @ Tue, 14 Jul 2020 11:02:05: #2 Since the d (42) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:02:05: #2 You may need to consider one of the other alternative d(s): 4,13,42,547,573 WARNING @ Tue, 14 Jul 2020 11:02:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:02:05: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:02:05: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:02:07: 4000000 INFO @ Tue, 14 Jul 2020 11:02:14: 5000000 INFO @ Tue, 14 Jul 2020 11:02:20: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:02:21: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:02:26: 7000000 INFO @ Tue, 14 Jul 2020 11:02:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:02:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:02:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.10_summits.bed INFO @ Tue, 14 Jul 2020 11:02:30: Done! pass1 - making usageList (13 chroms): 0 millis pass2 - checking and writing primary data (618 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:02:33: 8000000 INFO @ Tue, 14 Jul 2020 11:02:33: #1 tag size is determined as 57 bps INFO @ Tue, 14 Jul 2020 11:02:33: #1 tag size = 57 INFO @ Tue, 14 Jul 2020 11:02:33: #1 total tags in treatment: 8048140 INFO @ Tue, 14 Jul 2020 11:02:33: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:02:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:02:33: #1 tags after filtering in treatment: 8048140 INFO @ Tue, 14 Jul 2020 11:02:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:02:33: #1 finished! INFO @ Tue, 14 Jul 2020 11:02:33: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:02:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:02:34: #2 number of paired peaks: 253 WARNING @ Tue, 14 Jul 2020 11:02:34: Fewer paired peaks (253) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 253 pairs to build model! INFO @ Tue, 14 Jul 2020 11:02:34: start model_add_line... INFO @ Tue, 14 Jul 2020 11:02:34: start X-correlation... INFO @ Tue, 14 Jul 2020 11:02:34: end of X-cor INFO @ Tue, 14 Jul 2020 11:02:34: #2 finished! INFO @ Tue, 14 Jul 2020 11:02:34: #2 predicted fragment length is 42 bps INFO @ Tue, 14 Jul 2020 11:02:34: #2 alternative fragment length(s) may be 4,13,42,547,573 bps INFO @ Tue, 14 Jul 2020 11:02:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.20_model.r WARNING @ Tue, 14 Jul 2020 11:02:34: #2 Since the d (42) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:02:34: #2 You may need to consider one of the other alternative d(s): 4,13,42,547,573 WARNING @ Tue, 14 Jul 2020 11:02:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:02:34: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:02:34: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:02:50: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:02:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:02:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:02:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521321/SRX8521321.20_summits.bed INFO @ Tue, 14 Jul 2020 11:02:57: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (97 records, 4 fields): 1 millis CompletedMACS2peakCalling