Job ID = 6627190 SRX = SRX8521314 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:31:01 prefetch.2.10.7: 1) Downloading 'SRR11978725'... 2020-07-14T01:31:01 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:31:20 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:31:20 prefetch.2.10.7: 'SRR11978725' is valid 2020-07-14T01:31:20 prefetch.2.10.7: 1) 'SRR11978725' was downloaded successfully 2020-07-14T01:31:20 prefetch.2.10.7: 'SRR11978725' has 0 unresolved dependencies Read 294488 spots for SRR11978725/SRR11978725.sra Written 294488 spots for SRR11978725/SRR11978725.sra 2020-07-14T01:31:33 prefetch.2.10.7: 1) Downloading 'SRR11978726'... 2020-07-14T01:31:33 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:31:56 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:31:56 prefetch.2.10.7: 'SRR11978726' is valid 2020-07-14T01:31:56 prefetch.2.10.7: 1) 'SRR11978726' was downloaded successfully 2020-07-14T01:31:56 prefetch.2.10.7: 'SRR11978726' has 0 unresolved dependencies Read 522859 spots for SRR11978726/SRR11978726.sra Written 522859 spots for SRR11978726/SRR11978726.sra 2020-07-14T01:32:12 prefetch.2.10.7: 1) Downloading 'SRR11978727'... 2020-07-14T01:32:12 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:32:28 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:32:28 prefetch.2.10.7: 'SRR11978727' is valid 2020-07-14T01:32:28 prefetch.2.10.7: 1) 'SRR11978727' was downloaded successfully 2020-07-14T01:32:28 prefetch.2.10.7: 'SRR11978727' has 0 unresolved dependencies Read 556886 spots for SRR11978727/SRR11978727.sra Written 556886 spots for SRR11978727/SRR11978727.sra 2020-07-14T01:32:44 prefetch.2.10.7: 1) Downloading 'SRR11978728'... 2020-07-14T01:32:44 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:33:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:33:10 prefetch.2.10.7: 'SRR11978728' is valid 2020-07-14T01:33:10 prefetch.2.10.7: 1) 'SRR11978728' was downloaded successfully 2020-07-14T01:33:10 prefetch.2.10.7: 'SRR11978728' has 0 unresolved dependencies Read 545474 spots for SRR11978728/SRR11978728.sra Written 545474 spots for SRR11978728/SRR11978728.sra 2020-07-14T01:33:26 prefetch.2.10.7: 1) Downloading 'SRR11978729'... 2020-07-14T01:33:26 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:34:05 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:34:05 prefetch.2.10.7: 'SRR11978729' is valid 2020-07-14T01:34:05 prefetch.2.10.7: 1) 'SRR11978729' was downloaded successfully 2020-07-14T01:34:05 prefetch.2.10.7: 'SRR11978729' has 0 unresolved dependencies Read 3489152 spots for SRR11978729/SRR11978729.sra Written 3489152 spots for SRR11978729/SRR11978729.sra 2020-07-14T01:34:31 prefetch.2.10.7: 1) Downloading 'SRR11978730'... 2020-07-14T01:34:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:03 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:03 prefetch.2.10.7: 'SRR11978730' is valid 2020-07-14T01:35:03 prefetch.2.10.7: 1) 'SRR11978730' was downloaded successfully 2020-07-14T01:35:03 prefetch.2.10.7: 'SRR11978730' has 0 unresolved dependencies Read 3557864 spots for SRR11978730/SRR11978730.sra Written 3557864 spots for SRR11978730/SRR11978730.sra 2020-07-14T01:35:29 prefetch.2.10.7: 1) Downloading 'SRR11978731'... 2020-07-14T01:35:29 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:59 prefetch.2.10.7: 'SRR11978731' is valid 2020-07-14T01:35:59 prefetch.2.10.7: 1) 'SRR11978731' was downloaded successfully 2020-07-14T01:35:59 prefetch.2.10.7: 'SRR11978731' has 0 unresolved dependencies Read 3540749 spots for SRR11978731/SRR11978731.sra Written 3540749 spots for SRR11978731/SRR11978731.sra 2020-07-14T01:36:22 prefetch.2.10.7: 1) Downloading 'SRR11978732'... 2020-07-14T01:36:22 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:02 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:02 prefetch.2.10.7: 'SRR11978732' is valid 2020-07-14T01:37:02 prefetch.2.10.7: 1) 'SRR11978732' was downloaded successfully 2020-07-14T01:37:02 prefetch.2.10.7: 'SRR11978732' has 0 unresolved dependencies Read 3495064 spots for SRR11978732/SRR11978732.sra Written 3495064 spots for SRR11978732/SRR11978732.sra 2020-07-14T01:37:28 prefetch.2.10.7: 1) Downloading 'SRR11978733'... 2020-07-14T01:37:28 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:42 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:42 prefetch.2.10.7: 'SRR11978733' is valid 2020-07-14T01:37:42 prefetch.2.10.7: 1) 'SRR11978733' was downloaded successfully 2020-07-14T01:37:42 prefetch.2.10.7: 'SRR11978733' has 0 unresolved dependencies Read 282114 spots for SRR11978733/SRR11978733.sra Written 282114 spots for SRR11978733/SRR11978733.sra 2020-07-14T01:37:54 prefetch.2.10.7: 1) Downloading 'SRR11978734'... 2020-07-14T01:37:54 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:38:32 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:38:32 prefetch.2.10.7: 'SRR11978734' is valid 2020-07-14T01:38:32 prefetch.2.10.7: 1) 'SRR11978734' was downloaded successfully 2020-07-14T01:38:32 prefetch.2.10.7: 'SRR11978734' has 0 unresolved dependencies Read 295945 spots for SRR11978734/SRR11978734.sra Written 295945 spots for SRR11978734/SRR11978734.sra 2020-07-14T01:38:43 prefetch.2.10.7: 1) Downloading 'SRR11978735'... 2020-07-14T01:38:43 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:38:56 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:38:56 prefetch.2.10.7: 'SRR11978735' is valid 2020-07-14T01:38:56 prefetch.2.10.7: 1) 'SRR11978735' was downloaded successfully 2020-07-14T01:38:56 prefetch.2.10.7: 'SRR11978735' has 0 unresolved dependencies Read 298731 spots for SRR11978735/SRR11978735.sra Written 298731 spots for SRR11978735/SRR11978735.sra 2020-07-14T01:39:11 prefetch.2.10.7: 1) Downloading 'SRR11978736'... 2020-07-14T01:39:11 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:40:31 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:40:31 prefetch.2.10.7: 'SRR11978736' is valid 2020-07-14T01:40:31 prefetch.2.10.7: 1) 'SRR11978736' was downloaded successfully 2020-07-14T01:40:31 prefetch.2.10.7: 'SRR11978736' has 0 unresolved dependencies Read 9132595 spots for SRR11978736/SRR11978736.sra Written 9132595 spots for SRR11978736/SRR11978736.sra 2020-07-14T01:41:15 prefetch.2.10.7: 1) Downloading 'SRR11978737'... 2020-07-14T01:41:15 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:42:43 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:42:44 prefetch.2.10.7: 'SRR11978737' is valid 2020-07-14T01:42:44 prefetch.2.10.7: 1) 'SRR11978737' was downloaded successfully 2020-07-14T01:42:44 prefetch.2.10.7: 'SRR11978737' has 0 unresolved dependencies Read 8905263 spots for SRR11978737/SRR11978737.sra Written 8905263 spots for SRR11978737/SRR11978737.sra 2020-07-14T01:43:24 prefetch.2.10.7: 1) Downloading 'SRR11978738'... 2020-07-14T01:43:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:06 prefetch.2.10.7: 'SRR11978738' is valid 2020-07-14T01:45:06 prefetch.2.10.7: 1) 'SRR11978738' was downloaded successfully 2020-07-14T01:45:06 prefetch.2.10.7: 'SRR11978738' has 0 unresolved dependencies Read 9235235 spots for SRR11978738/SRR11978738.sra Written 9235235 spots for SRR11978738/SRR11978738.sra 2020-07-14T01:45:50 prefetch.2.10.7: 1) Downloading 'SRR11978739'... 2020-07-14T01:45:50 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:01 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:02 prefetch.2.10.7: 'SRR11978739' is valid 2020-07-14T01:47:02 prefetch.2.10.7: 1) 'SRR11978739' was downloaded successfully 2020-07-14T01:47:02 prefetch.2.10.7: 'SRR11978739' has 0 unresolved dependencies Read 9021335 spots for SRR11978739/SRR11978739.sra Written 9021335 spots for SRR11978739/SRR11978739.sra 2020-07-14T01:47:45 prefetch.2.10.7: 1) Downloading 'SRR11978740'... 2020-07-14T01:47:45 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:48:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:48:06 prefetch.2.10.7: 'SRR11978740' is valid 2020-07-14T01:48:06 prefetch.2.10.7: 1) 'SRR11978740' was downloaded successfully 2020-07-14T01:48:06 prefetch.2.10.7: 'SRR11978740' has 0 unresolved dependencies Read 543257 spots for SRR11978740/SRR11978740.sra Written 543257 spots for SRR11978740/SRR11978740.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627471 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:50 53717011 reads; of these: 53717011 (100.00%) were unpaired; of these: 46846979 (87.21%) aligned 0 times 5268191 (9.81%) aligned exactly 1 time 1601841 (2.98%) aligned >1 times 12.79% overall alignment rate Time searching: 00:08:50 Overall time: 00:08:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1502180 / 6870032 = 0.2187 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:59:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:59:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:00:00: 1000000 INFO @ Tue, 14 Jul 2020 11:00:06: 2000000 INFO @ Tue, 14 Jul 2020 11:00:12: 3000000 INFO @ Tue, 14 Jul 2020 11:00:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:00:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:00:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:00:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:00:24: 5000000 INFO @ Tue, 14 Jul 2020 11:00:27: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 11:00:27: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 11:00:27: #1 total tags in treatment: 5367852 INFO @ Tue, 14 Jul 2020 11:00:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:00:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:00:27: #1 tags after filtering in treatment: 5367852 INFO @ Tue, 14 Jul 2020 11:00:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:00:27: #1 finished! INFO @ Tue, 14 Jul 2020 11:00:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:00:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:00:27: #2 number of paired peaks: 429 WARNING @ Tue, 14 Jul 2020 11:00:27: Fewer paired peaks (429) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 429 pairs to build model! INFO @ Tue, 14 Jul 2020 11:00:27: start model_add_line... INFO @ Tue, 14 Jul 2020 11:00:27: start X-correlation... INFO @ Tue, 14 Jul 2020 11:00:27: end of X-cor INFO @ Tue, 14 Jul 2020 11:00:27: #2 finished! INFO @ Tue, 14 Jul 2020 11:00:27: #2 predicted fragment length is 60 bps INFO @ Tue, 14 Jul 2020 11:00:27: #2 alternative fragment length(s) may be 60,567 bps INFO @ Tue, 14 Jul 2020 11:00:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.05_model.r WARNING @ Tue, 14 Jul 2020 11:00:27: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:00:27: #2 You may need to consider one of the other alternative d(s): 60,567 WARNING @ Tue, 14 Jul 2020 11:00:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:00:27: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:00:27: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:00:31: 1000000 INFO @ Tue, 14 Jul 2020 11:00:37: 2000000 INFO @ Tue, 14 Jul 2020 11:00:40: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:00:43: 3000000 INFO @ Tue, 14 Jul 2020 11:00:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:00:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:00:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.05_summits.bed INFO @ Tue, 14 Jul 2020 11:00:46: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1654 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:00:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:00:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:00:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:00:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:00:56: 5000000 INFO @ Tue, 14 Jul 2020 11:00:58: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 11:00:58: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 11:00:58: #1 total tags in treatment: 5367852 INFO @ Tue, 14 Jul 2020 11:00:58: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:00:59: #1 tags after filtering in treatment: 5367852 INFO @ Tue, 14 Jul 2020 11:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:00:59: #1 finished! INFO @ Tue, 14 Jul 2020 11:00:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:00:59: #2 number of paired peaks: 429 WARNING @ Tue, 14 Jul 2020 11:00:59: Fewer paired peaks (429) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 429 pairs to build model! INFO @ Tue, 14 Jul 2020 11:00:59: start model_add_line... INFO @ Tue, 14 Jul 2020 11:00:59: start X-correlation... INFO @ Tue, 14 Jul 2020 11:00:59: end of X-cor INFO @ Tue, 14 Jul 2020 11:00:59: #2 finished! INFO @ Tue, 14 Jul 2020 11:00:59: #2 predicted fragment length is 60 bps INFO @ Tue, 14 Jul 2020 11:00:59: #2 alternative fragment length(s) may be 60,567 bps INFO @ Tue, 14 Jul 2020 11:00:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.10_model.r WARNING @ Tue, 14 Jul 2020 11:00:59: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:00:59: #2 You may need to consider one of the other alternative d(s): 60,567 WARNING @ Tue, 14 Jul 2020 11:00:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:00:59: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:00:59: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:01:01: 1000000 INFO @ Tue, 14 Jul 2020 11:01:07: 2000000 INFO @ Tue, 14 Jul 2020 11:01:12: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:01:13: 3000000 INFO @ Tue, 14 Jul 2020 11:01:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:01:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:01:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.10_summits.bed INFO @ Tue, 14 Jul 2020 11:01:19: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (672 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:01:20: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:01:26: 5000000 INFO @ Tue, 14 Jul 2020 11:01:28: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 11:01:28: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 11:01:28: #1 total tags in treatment: 5367852 INFO @ Tue, 14 Jul 2020 11:01:28: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:01:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:01:29: #1 tags after filtering in treatment: 5367852 INFO @ Tue, 14 Jul 2020 11:01:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:01:29: #1 finished! INFO @ Tue, 14 Jul 2020 11:01:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:01:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:01:29: #2 number of paired peaks: 429 WARNING @ Tue, 14 Jul 2020 11:01:29: Fewer paired peaks (429) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 429 pairs to build model! INFO @ Tue, 14 Jul 2020 11:01:29: start model_add_line... INFO @ Tue, 14 Jul 2020 11:01:29: start X-correlation... INFO @ Tue, 14 Jul 2020 11:01:29: end of X-cor INFO @ Tue, 14 Jul 2020 11:01:29: #2 finished! INFO @ Tue, 14 Jul 2020 11:01:29: #2 predicted fragment length is 60 bps INFO @ Tue, 14 Jul 2020 11:01:29: #2 alternative fragment length(s) may be 60,567 bps INFO @ Tue, 14 Jul 2020 11:01:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.20_model.r WARNING @ Tue, 14 Jul 2020 11:01:29: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:01:29: #2 You may need to consider one of the other alternative d(s): 60,567 WARNING @ Tue, 14 Jul 2020 11:01:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:01:29: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:01:29: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:01:42: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:01:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:01:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:01:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521314/SRX8521314.20_summits.bed INFO @ Tue, 14 Jul 2020 11:01:48: Done! pass1 - making usageList (9 chroms): 0 millis pass2 - checking and writing primary data (172 records, 4 fields): 2 millis CompletedMACS2peakCalling