Job ID = 14170620 SRX = SRX8497064 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16258451 spots for SRR11952663/SRR11952663.sra Written 16258451 spots for SRR11952663/SRR11952663.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171163 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:37 16258451 reads; of these: 16258451 (100.00%) were paired; of these: 1546996 (9.52%) aligned concordantly 0 times 10891719 (66.99%) aligned concordantly exactly 1 time 3819736 (23.49%) aligned concordantly >1 times ---- 1546996 pairs aligned concordantly 0 times; of these: 494174 (31.94%) aligned discordantly 1 time ---- 1052822 pairs aligned 0 times concordantly or discordantly; of these: 2105644 mates make up the pairs; of these: 1365533 (64.85%) aligned 0 times 329049 (15.63%) aligned exactly 1 time 411062 (19.52%) aligned >1 times 95.80% overall alignment rate Time searching: 00:44:37 Overall time: 00:44:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 944108 / 15134637 = 0.0624 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:29:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:29:07: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:29:07: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:29:14: 1000000 INFO @ Sat, 11 Dec 2021 08:29:21: 2000000 INFO @ Sat, 11 Dec 2021 08:29:29: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:29:37: 4000000 INFO @ Sat, 11 Dec 2021 08:29:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:29:37: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:29:37: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:29:45: 1000000 INFO @ Sat, 11 Dec 2021 08:29:45: 5000000 INFO @ Sat, 11 Dec 2021 08:29:52: 2000000 INFO @ Sat, 11 Dec 2021 08:29:53: 6000000 INFO @ Sat, 11 Dec 2021 08:30:00: 3000000 INFO @ Sat, 11 Dec 2021 08:30:01: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 08:30:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 08:30:07: #1 read tag files... INFO @ Sat, 11 Dec 2021 08:30:07: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 08:30:07: 4000000 INFO @ Sat, 11 Dec 2021 08:30:08: 8000000 INFO @ Sat, 11 Dec 2021 08:30:14: 1000000 INFO @ Sat, 11 Dec 2021 08:30:15: 5000000 INFO @ Sat, 11 Dec 2021 08:30:16: 9000000 INFO @ Sat, 11 Dec 2021 08:30:22: 2000000 INFO @ Sat, 11 Dec 2021 08:30:22: 6000000 INFO @ Sat, 11 Dec 2021 08:30:24: 10000000 INFO @ Sat, 11 Dec 2021 08:30:30: 3000000 INFO @ Sat, 11 Dec 2021 08:30:30: 7000000 INFO @ Sat, 11 Dec 2021 08:30:32: 11000000 INFO @ Sat, 11 Dec 2021 08:30:38: 8000000 INFO @ Sat, 11 Dec 2021 08:30:38: 4000000 INFO @ Sat, 11 Dec 2021 08:30:40: 12000000 INFO @ Sat, 11 Dec 2021 08:30:46: 9000000 INFO @ Sat, 11 Dec 2021 08:30:46: 5000000 INFO @ Sat, 11 Dec 2021 08:30:48: 13000000 INFO @ Sat, 11 Dec 2021 08:30:54: 10000000 INFO @ Sat, 11 Dec 2021 08:30:54: 6000000 INFO @ Sat, 11 Dec 2021 08:30:56: 14000000 INFO @ Sat, 11 Dec 2021 08:31:02: 11000000 INFO @ Sat, 11 Dec 2021 08:31:02: 7000000 INFO @ Sat, 11 Dec 2021 08:31:04: 15000000 INFO @ Sat, 11 Dec 2021 08:31:10: 12000000 INFO @ Sat, 11 Dec 2021 08:31:10: 8000000 INFO @ Sat, 11 Dec 2021 08:31:12: 16000000 INFO @ Sat, 11 Dec 2021 08:31:18: 13000000 INFO @ Sat, 11 Dec 2021 08:31:18: 9000000 INFO @ Sat, 11 Dec 2021 08:31:20: 17000000 INFO @ Sat, 11 Dec 2021 08:31:25: 14000000 INFO @ Sat, 11 Dec 2021 08:31:26: 10000000 INFO @ Sat, 11 Dec 2021 08:31:28: 18000000 INFO @ Sat, 11 Dec 2021 08:31:32: 15000000 INFO @ Sat, 11 Dec 2021 08:31:34: 11000000 INFO @ Sat, 11 Dec 2021 08:31:35: 19000000 INFO @ Sat, 11 Dec 2021 08:31:40: 16000000 INFO @ Sat, 11 Dec 2021 08:31:41: 12000000 INFO @ Sat, 11 Dec 2021 08:31:43: 20000000 INFO @ Sat, 11 Dec 2021 08:31:47: 17000000 INFO @ Sat, 11 Dec 2021 08:31:49: 13000000 INFO @ Sat, 11 Dec 2021 08:31:51: 21000000 INFO @ Sat, 11 Dec 2021 08:31:56: 18000000 INFO @ Sat, 11 Dec 2021 08:31:58: 14000000 INFO @ Sat, 11 Dec 2021 08:31:59: 22000000 INFO @ Sat, 11 Dec 2021 08:32:04: 19000000 INFO @ Sat, 11 Dec 2021 08:32:06: 15000000 INFO @ Sat, 11 Dec 2021 08:32:08: 23000000 INFO @ Sat, 11 Dec 2021 08:32:12: 20000000 INFO @ Sat, 11 Dec 2021 08:32:14: 16000000 INFO @ Sat, 11 Dec 2021 08:32:16: 24000000 INFO @ Sat, 11 Dec 2021 08:32:20: 21000000 INFO @ Sat, 11 Dec 2021 08:32:22: 17000000 INFO @ Sat, 11 Dec 2021 08:32:24: 25000000 INFO @ Sat, 11 Dec 2021 08:32:27: 22000000 INFO @ Sat, 11 Dec 2021 08:32:30: 18000000 INFO @ Sat, 11 Dec 2021 08:32:32: 26000000 INFO @ Sat, 11 Dec 2021 08:32:35: 23000000 INFO @ Sat, 11 Dec 2021 08:32:38: 19000000 INFO @ Sat, 11 Dec 2021 08:32:40: 27000000 INFO @ Sat, 11 Dec 2021 08:32:43: 24000000 BedGraph に変換しました。 INFO @ Sat, 11 Dec 2021 08:32:45: 20000000 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:32:47: 28000000 INFO @ Sat, 11 Dec 2021 08:32:50: 25000000 INFO @ Sat, 11 Dec 2021 08:32:53: 21000000 INFO @ Sat, 11 Dec 2021 08:32:55: 29000000 INFO @ Sat, 11 Dec 2021 08:32:57: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 08:32:57: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 08:32:57: #1 total tags in treatment: 13789611 INFO @ Sat, 11 Dec 2021 08:32:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:32:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:32:58: #1 tags after filtering in treatment: 13116075 INFO @ Sat, 11 Dec 2021 08:32:58: #1 Redundant rate of treatment: 0.05 INFO @ Sat, 11 Dec 2021 08:32:58: #1 finished! INFO @ Sat, 11 Dec 2021 08:32:58: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:32:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:32:58: 26000000 INFO @ Sat, 11 Dec 2021 08:32:58: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 08:32:59: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:32:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:33:00: 22000000 INFO @ Sat, 11 Dec 2021 08:33:05: 27000000 INFO @ Sat, 11 Dec 2021 08:33:08: 23000000 INFO @ Sat, 11 Dec 2021 08:33:13: 28000000 INFO @ Sat, 11 Dec 2021 08:33:17: 24000000 INFO @ Sat, 11 Dec 2021 08:33:21: 29000000 INFO @ Sat, 11 Dec 2021 08:33:23: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 08:33:24: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 08:33:24: #1 total tags in treatment: 13789611 INFO @ Sat, 11 Dec 2021 08:33:24: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:33:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:33:24: #1 tags after filtering in treatment: 13116075 INFO @ Sat, 11 Dec 2021 08:33:24: #1 Redundant rate of treatment: 0.05 INFO @ Sat, 11 Dec 2021 08:33:24: #1 finished! INFO @ Sat, 11 Dec 2021 08:33:24: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:33:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:33:25: 25000000 INFO @ Sat, 11 Dec 2021 08:33:25: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 08:33:25: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:33:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:33:32: 26000000 INFO @ Sat, 11 Dec 2021 08:33:40: 27000000 INFO @ Sat, 11 Dec 2021 08:33:47: 28000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:33:55: 29000000 INFO @ Sat, 11 Dec 2021 08:33:57: #1 tag size is determined as 101 bps INFO @ Sat, 11 Dec 2021 08:33:57: #1 tag size = 101 INFO @ Sat, 11 Dec 2021 08:33:57: #1 total tags in treatment: 13789611 INFO @ Sat, 11 Dec 2021 08:33:57: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:33:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:33:57: #1 tags after filtering in treatment: 13116075 INFO @ Sat, 11 Dec 2021 08:33:57: #1 Redundant rate of treatment: 0.05 INFO @ Sat, 11 Dec 2021 08:33:57: #1 finished! INFO @ Sat, 11 Dec 2021 08:33:57: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:33:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:33:58: #2 number of paired peaks: 92 WARNING @ Sat, 11 Dec 2021 08:33:58: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:33:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8497064/SRX8497064.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling