Job ID = 16439719 SRX = SRX8369902 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 88214348 spots for SRR11818938/SRR11818938.sra Written 88214348 spots for SRR11818938/SRR11818938.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440036 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:06 88214348 reads; of these: 88214348 (100.00%) were unpaired; of these: 22777821 (25.82%) aligned 0 times 49911030 (56.58%) aligned exactly 1 time 15525497 (17.60%) aligned >1 times 74.18% overall alignment rate Time searching: 00:32:06 Overall time: 00:32:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 17499699 / 65436527 = 0.2674 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:55:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:55:16: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:55:16: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:27: 1000000 INFO @ Tue, 02 Aug 2022 16:55:38: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:55:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:55:46: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:55:46: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:55:50: 3000000 INFO @ Tue, 02 Aug 2022 16:55:56: 1000000 INFO @ Tue, 02 Aug 2022 16:56:00: 4000000 INFO @ Tue, 02 Aug 2022 16:56:06: 2000000 INFO @ Tue, 02 Aug 2022 16:56:11: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:56:16: 3000000 INFO @ Tue, 02 Aug 2022 16:56:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:56:16: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:56:16: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:56:23: 6000000 INFO @ Tue, 02 Aug 2022 16:56:27: 4000000 INFO @ Tue, 02 Aug 2022 16:56:27: 1000000 INFO @ Tue, 02 Aug 2022 16:56:35: 7000000 INFO @ Tue, 02 Aug 2022 16:56:37: 5000000 INFO @ Tue, 02 Aug 2022 16:56:38: 2000000 INFO @ Tue, 02 Aug 2022 16:56:47: 8000000 INFO @ Tue, 02 Aug 2022 16:56:47: 6000000 INFO @ Tue, 02 Aug 2022 16:56:48: 3000000 INFO @ Tue, 02 Aug 2022 16:56:57: 7000000 INFO @ Tue, 02 Aug 2022 16:56:58: 4000000 INFO @ Tue, 02 Aug 2022 16:56:58: 9000000 INFO @ Tue, 02 Aug 2022 16:57:08: 8000000 INFO @ Tue, 02 Aug 2022 16:57:08: 5000000 INFO @ Tue, 02 Aug 2022 16:57:10: 10000000 INFO @ Tue, 02 Aug 2022 16:57:18: 9000000 INFO @ Tue, 02 Aug 2022 16:57:18: 6000000 INFO @ Tue, 02 Aug 2022 16:57:21: 11000000 INFO @ Tue, 02 Aug 2022 16:57:28: 7000000 INFO @ Tue, 02 Aug 2022 16:57:28: 10000000 INFO @ Tue, 02 Aug 2022 16:57:33: 12000000 INFO @ Tue, 02 Aug 2022 16:57:38: 8000000 INFO @ Tue, 02 Aug 2022 16:57:39: 11000000 INFO @ Tue, 02 Aug 2022 16:57:45: 13000000 INFO @ Tue, 02 Aug 2022 16:57:48: 9000000 INFO @ Tue, 02 Aug 2022 16:57:49: 12000000 INFO @ Tue, 02 Aug 2022 16:57:57: 14000000 INFO @ Tue, 02 Aug 2022 16:57:59: 10000000 INFO @ Tue, 02 Aug 2022 16:57:59: 13000000 INFO @ Tue, 02 Aug 2022 16:58:09: 15000000 INFO @ Tue, 02 Aug 2022 16:58:09: 11000000 INFO @ Tue, 02 Aug 2022 16:58:10: 14000000 INFO @ Tue, 02 Aug 2022 16:58:19: 12000000 INFO @ Tue, 02 Aug 2022 16:58:20: 16000000 INFO @ Tue, 02 Aug 2022 16:58:20: 15000000 INFO @ Tue, 02 Aug 2022 16:58:29: 13000000 INFO @ Tue, 02 Aug 2022 16:58:31: 16000000 INFO @ Tue, 02 Aug 2022 16:58:32: 17000000 INFO @ Tue, 02 Aug 2022 16:58:39: 14000000 INFO @ Tue, 02 Aug 2022 16:58:42: 17000000 INFO @ Tue, 02 Aug 2022 16:58:44: 18000000 INFO @ Tue, 02 Aug 2022 16:58:49: 15000000 INFO @ Tue, 02 Aug 2022 16:58:52: 18000000 INFO @ Tue, 02 Aug 2022 16:58:55: 19000000 INFO @ Tue, 02 Aug 2022 16:59:00: 16000000 INFO @ Tue, 02 Aug 2022 16:59:03: 19000000 INFO @ Tue, 02 Aug 2022 16:59:06: 20000000 INFO @ Tue, 02 Aug 2022 16:59:11: 17000000 INFO @ Tue, 02 Aug 2022 16:59:14: 20000000 INFO @ Tue, 02 Aug 2022 16:59:18: 21000000 INFO @ Tue, 02 Aug 2022 16:59:22: 18000000 INFO @ Tue, 02 Aug 2022 16:59:25: 21000000 INFO @ Tue, 02 Aug 2022 16:59:29: 22000000 INFO @ Tue, 02 Aug 2022 16:59:32: 19000000 INFO @ Tue, 02 Aug 2022 16:59:36: 22000000 INFO @ Tue, 02 Aug 2022 16:59:39: 23000000 INFO @ Tue, 02 Aug 2022 16:59:42: 20000000 INFO @ Tue, 02 Aug 2022 16:59:47: 23000000 INFO @ Tue, 02 Aug 2022 16:59:51: 24000000 INFO @ Tue, 02 Aug 2022 16:59:52: 21000000 INFO @ Tue, 02 Aug 2022 16:59:58: 24000000 INFO @ Tue, 02 Aug 2022 17:00:02: 22000000 INFO @ Tue, 02 Aug 2022 17:00:02: 25000000 INFO @ Tue, 02 Aug 2022 17:00:08: 25000000 INFO @ Tue, 02 Aug 2022 17:00:12: 23000000 INFO @ Tue, 02 Aug 2022 17:00:13: 26000000 INFO @ Tue, 02 Aug 2022 17:00:17: 26000000 INFO @ Tue, 02 Aug 2022 17:00:22: 24000000 INFO @ Tue, 02 Aug 2022 17:00:25: 27000000 INFO @ Tue, 02 Aug 2022 17:00:28: 27000000 INFO @ Tue, 02 Aug 2022 17:00:32: 25000000 INFO @ Tue, 02 Aug 2022 17:00:36: 28000000 INFO @ Tue, 02 Aug 2022 17:00:38: 28000000 INFO @ Tue, 02 Aug 2022 17:00:42: 26000000 INFO @ Tue, 02 Aug 2022 17:00:47: 29000000 INFO @ Tue, 02 Aug 2022 17:00:48: 29000000 INFO @ Tue, 02 Aug 2022 17:00:52: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:00:58: 30000000 INFO @ Tue, 02 Aug 2022 17:00:59: 30000000 INFO @ Tue, 02 Aug 2022 17:01:02: 28000000 INFO @ Tue, 02 Aug 2022 17:01:09: 31000000 INFO @ Tue, 02 Aug 2022 17:01:10: 31000000 INFO @ Tue, 02 Aug 2022 17:01:12: 29000000 INFO @ Tue, 02 Aug 2022 17:01:20: 32000000 INFO @ Tue, 02 Aug 2022 17:01:21: 32000000 INFO @ Tue, 02 Aug 2022 17:01:22: 30000000 INFO @ Tue, 02 Aug 2022 17:01:32: 31000000 INFO @ Tue, 02 Aug 2022 17:01:32: 33000000 INFO @ Tue, 02 Aug 2022 17:01:32: 33000000 INFO @ Tue, 02 Aug 2022 17:01:42: 32000000 INFO @ Tue, 02 Aug 2022 17:01:43: 34000000 INFO @ Tue, 02 Aug 2022 17:01:43: 34000000 INFO @ Tue, 02 Aug 2022 17:01:52: 33000000 INFO @ Tue, 02 Aug 2022 17:01:53: 35000000 INFO @ Tue, 02 Aug 2022 17:01:54: 35000000 INFO @ Tue, 02 Aug 2022 17:02:02: 34000000 INFO @ Tue, 02 Aug 2022 17:02:04: 36000000 INFO @ Tue, 02 Aug 2022 17:02:05: 36000000 INFO @ Tue, 02 Aug 2022 17:02:12: 35000000 INFO @ Tue, 02 Aug 2022 17:02:15: 37000000 INFO @ Tue, 02 Aug 2022 17:02:17: 37000000 INFO @ Tue, 02 Aug 2022 17:02:24: 36000000 INFO @ Tue, 02 Aug 2022 17:02:27: 38000000 INFO @ Tue, 02 Aug 2022 17:02:28: 38000000 INFO @ Tue, 02 Aug 2022 17:02:34: 37000000 INFO @ Tue, 02 Aug 2022 17:02:38: 39000000 INFO @ Tue, 02 Aug 2022 17:02:40: 39000000 INFO @ Tue, 02 Aug 2022 17:02:46: 38000000 INFO @ Tue, 02 Aug 2022 17:02:49: 40000000 INFO @ Tue, 02 Aug 2022 17:02:51: 40000000 INFO @ Tue, 02 Aug 2022 17:02:56: 39000000 INFO @ Tue, 02 Aug 2022 17:03:00: 41000000 INFO @ Tue, 02 Aug 2022 17:03:02: 41000000 INFO @ Tue, 02 Aug 2022 17:03:06: 40000000 INFO @ Tue, 02 Aug 2022 17:03:11: 42000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:03:13: 42000000 INFO @ Tue, 02 Aug 2022 17:03:16: 41000000 INFO @ Tue, 02 Aug 2022 17:03:21: 43000000 INFO @ Tue, 02 Aug 2022 17:03:24: 43000000 INFO @ Tue, 02 Aug 2022 17:03:26: 42000000 INFO @ Tue, 02 Aug 2022 17:03:32: 44000000 INFO @ Tue, 02 Aug 2022 17:03:35: 44000000 INFO @ Tue, 02 Aug 2022 17:03:36: 43000000 INFO @ Tue, 02 Aug 2022 17:03:42: 45000000 INFO @ Tue, 02 Aug 2022 17:03:45: 45000000 INFO @ Tue, 02 Aug 2022 17:03:46: 44000000 INFO @ Tue, 02 Aug 2022 17:03:53: 46000000 INFO @ Tue, 02 Aug 2022 17:03:56: 46000000 INFO @ Tue, 02 Aug 2022 17:03:56: 45000000 INFO @ Tue, 02 Aug 2022 17:04:04: 47000000 INFO @ Tue, 02 Aug 2022 17:04:06: 46000000 INFO @ Tue, 02 Aug 2022 17:04:07: 47000000 INFO @ Tue, 02 Aug 2022 17:04:14: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:04:14: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:04:14: #1 total tags in treatment: 47936828 INFO @ Tue, 02 Aug 2022 17:04:14: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:04:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:04:15: #1 tags after filtering in treatment: 47936828 INFO @ Tue, 02 Aug 2022 17:04:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:04:15: #1 finished! INFO @ Tue, 02 Aug 2022 17:04:15: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:04:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:04:17: 47000000 INFO @ Tue, 02 Aug 2022 17:04:18: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:04:18: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:04:18: #1 total tags in treatment: 47936828 INFO @ Tue, 02 Aug 2022 17:04:18: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:04:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:04:19: #1 tags after filtering in treatment: 47936828 INFO @ Tue, 02 Aug 2022 17:04:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:04:19: #1 finished! INFO @ Tue, 02 Aug 2022 17:04:19: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:04:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:04:19: #2 number of paired peaks: 1 WARNING @ Tue, 02 Aug 2022 17:04:19: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:04:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:04:23: #2 number of paired peaks: 1 WARNING @ Tue, 02 Aug 2022 17:04:23: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:04:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:04:28: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:04:28: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:04:28: #1 total tags in treatment: 47936828 INFO @ Tue, 02 Aug 2022 17:04:28: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:04:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:04:29: #1 tags after filtering in treatment: 47936828 INFO @ Tue, 02 Aug 2022 17:04:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:04:29: #1 finished! INFO @ Tue, 02 Aug 2022 17:04:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:04:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:04:33: #2 number of paired peaks: 1 WARNING @ Tue, 02 Aug 2022 17:04:33: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:04:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369902/SRX8369902.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling