Job ID = 16439811 SRX = SRX8369899 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 77462355 spots for SRR11818935/SRR11818935.sra Written 77462355 spots for SRR11818935/SRR11818935.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440061 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:45 77462355 reads; of these: 77462355 (100.00%) were unpaired; of these: 2590199 (3.34%) aligned 0 times 55268342 (71.35%) aligned exactly 1 time 19603814 (25.31%) aligned >1 times 96.66% overall alignment rate Time searching: 00:28:45 Overall time: 00:28:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 17390414 / 74872156 = 0.2323 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:59:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:59:56: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:59:56: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:00:02: 1000000 INFO @ Tue, 02 Aug 2022 17:00:08: 2000000 INFO @ Tue, 02 Aug 2022 17:00:14: 3000000 INFO @ Tue, 02 Aug 2022 17:00:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:00:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:00:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:00:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:00:26: 5000000 INFO @ Tue, 02 Aug 2022 17:00:31: 1000000 INFO @ Tue, 02 Aug 2022 17:00:32: 6000000 INFO @ Tue, 02 Aug 2022 17:00:37: 2000000 INFO @ Tue, 02 Aug 2022 17:00:39: 7000000 INFO @ Tue, 02 Aug 2022 17:00:43: 3000000 INFO @ Tue, 02 Aug 2022 17:00:45: 8000000 INFO @ Tue, 02 Aug 2022 17:00:49: 4000000 INFO @ Tue, 02 Aug 2022 17:00:51: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:00:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:00:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:00:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:00:55: 5000000 INFO @ Tue, 02 Aug 2022 17:00:57: 10000000 INFO @ Tue, 02 Aug 2022 17:01:02: 1000000 INFO @ Tue, 02 Aug 2022 17:01:02: 6000000 INFO @ Tue, 02 Aug 2022 17:01:04: 11000000 INFO @ Tue, 02 Aug 2022 17:01:08: 7000000 INFO @ Tue, 02 Aug 2022 17:01:09: 2000000 INFO @ Tue, 02 Aug 2022 17:01:11: 12000000 INFO @ Tue, 02 Aug 2022 17:01:15: 8000000 INFO @ Tue, 02 Aug 2022 17:01:16: 3000000 INFO @ Tue, 02 Aug 2022 17:01:17: 13000000 INFO @ Tue, 02 Aug 2022 17:01:21: 9000000 INFO @ Tue, 02 Aug 2022 17:01:22: 4000000 INFO @ Tue, 02 Aug 2022 17:01:24: 14000000 INFO @ Tue, 02 Aug 2022 17:01:27: 10000000 INFO @ Tue, 02 Aug 2022 17:01:29: 5000000 INFO @ Tue, 02 Aug 2022 17:01:30: 15000000 INFO @ Tue, 02 Aug 2022 17:01:34: 11000000 INFO @ Tue, 02 Aug 2022 17:01:35: 6000000 INFO @ Tue, 02 Aug 2022 17:01:37: 16000000 INFO @ Tue, 02 Aug 2022 17:01:40: 12000000 INFO @ Tue, 02 Aug 2022 17:01:42: 7000000 INFO @ Tue, 02 Aug 2022 17:01:43: 17000000 INFO @ Tue, 02 Aug 2022 17:01:46: 13000000 INFO @ Tue, 02 Aug 2022 17:01:49: 8000000 INFO @ Tue, 02 Aug 2022 17:01:50: 18000000 INFO @ Tue, 02 Aug 2022 17:01:52: 14000000 INFO @ Tue, 02 Aug 2022 17:01:55: 9000000 INFO @ Tue, 02 Aug 2022 17:01:56: 19000000 INFO @ Tue, 02 Aug 2022 17:01:58: 15000000 INFO @ Tue, 02 Aug 2022 17:02:01: 10000000 INFO @ Tue, 02 Aug 2022 17:02:02: 20000000 INFO @ Tue, 02 Aug 2022 17:02:05: 16000000 INFO @ Tue, 02 Aug 2022 17:02:08: 11000000 INFO @ Tue, 02 Aug 2022 17:02:09: 21000000 INFO @ Tue, 02 Aug 2022 17:02:11: 17000000 INFO @ Tue, 02 Aug 2022 17:02:14: 12000000 INFO @ Tue, 02 Aug 2022 17:02:15: 22000000 INFO @ Tue, 02 Aug 2022 17:02:17: 18000000 INFO @ Tue, 02 Aug 2022 17:02:21: 13000000 INFO @ Tue, 02 Aug 2022 17:02:22: 23000000 INFO @ Tue, 02 Aug 2022 17:02:23: 19000000 INFO @ Tue, 02 Aug 2022 17:02:27: 14000000 INFO @ Tue, 02 Aug 2022 17:02:28: 24000000 INFO @ Tue, 02 Aug 2022 17:02:29: 20000000 INFO @ Tue, 02 Aug 2022 17:02:34: 15000000 INFO @ Tue, 02 Aug 2022 17:02:35: 21000000 INFO @ Tue, 02 Aug 2022 17:02:35: 25000000 INFO @ Tue, 02 Aug 2022 17:02:40: 16000000 INFO @ Tue, 02 Aug 2022 17:02:41: 22000000 INFO @ Tue, 02 Aug 2022 17:02:41: 26000000 INFO @ Tue, 02 Aug 2022 17:02:46: 17000000 INFO @ Tue, 02 Aug 2022 17:02:47: 23000000 INFO @ Tue, 02 Aug 2022 17:02:48: 27000000 INFO @ Tue, 02 Aug 2022 17:02:53: 18000000 INFO @ Tue, 02 Aug 2022 17:02:53: 24000000 INFO @ Tue, 02 Aug 2022 17:02:54: 28000000 INFO @ Tue, 02 Aug 2022 17:02:59: 25000000 INFO @ Tue, 02 Aug 2022 17:02:59: 19000000 INFO @ Tue, 02 Aug 2022 17:03:01: 29000000 INFO @ Tue, 02 Aug 2022 17:03:05: 26000000 INFO @ Tue, 02 Aug 2022 17:03:06: 20000000 INFO @ Tue, 02 Aug 2022 17:03:07: 30000000 INFO @ Tue, 02 Aug 2022 17:03:11: 27000000 INFO @ Tue, 02 Aug 2022 17:03:12: 21000000 INFO @ Tue, 02 Aug 2022 17:03:13: 31000000 INFO @ Tue, 02 Aug 2022 17:03:17: 28000000 INFO @ Tue, 02 Aug 2022 17:03:18: 22000000 INFO @ Tue, 02 Aug 2022 17:03:19: 32000000 INFO @ Tue, 02 Aug 2022 17:03:23: 29000000 INFO @ Tue, 02 Aug 2022 17:03:24: 23000000 INFO @ Tue, 02 Aug 2022 17:03:25: 33000000 INFO @ Tue, 02 Aug 2022 17:03:29: 30000000 INFO @ Tue, 02 Aug 2022 17:03:30: 24000000 INFO @ Tue, 02 Aug 2022 17:03:31: 34000000 INFO @ Tue, 02 Aug 2022 17:03:35: 31000000 INFO @ Tue, 02 Aug 2022 17:03:36: 25000000 INFO @ Tue, 02 Aug 2022 17:03:37: 35000000 INFO @ Tue, 02 Aug 2022 17:03:41: 32000000 INFO @ Tue, 02 Aug 2022 17:03:42: 26000000 INFO @ Tue, 02 Aug 2022 17:03:44: 36000000 INFO @ Tue, 02 Aug 2022 17:03:47: 33000000 INFO @ Tue, 02 Aug 2022 17:03:48: 27000000 INFO @ Tue, 02 Aug 2022 17:03:50: 37000000 INFO @ Tue, 02 Aug 2022 17:03:53: 34000000 INFO @ Tue, 02 Aug 2022 17:03:54: 28000000 INFO @ Tue, 02 Aug 2022 17:03:56: 38000000 INFO @ Tue, 02 Aug 2022 17:03:59: 35000000 INFO @ Tue, 02 Aug 2022 17:04:00: 29000000 INFO @ Tue, 02 Aug 2022 17:04:02: 39000000 INFO @ Tue, 02 Aug 2022 17:04:05: 36000000 INFO @ Tue, 02 Aug 2022 17:04:07: 30000000 INFO @ Tue, 02 Aug 2022 17:04:08: 40000000 INFO @ Tue, 02 Aug 2022 17:04:11: 37000000 INFO @ Tue, 02 Aug 2022 17:04:13: 31000000 INFO @ Tue, 02 Aug 2022 17:04:15: 41000000 INFO @ Tue, 02 Aug 2022 17:04:17: 38000000 INFO @ Tue, 02 Aug 2022 17:04:20: 32000000 INFO @ Tue, 02 Aug 2022 17:04:21: 42000000 INFO @ Tue, 02 Aug 2022 17:04:23: 39000000 INFO @ Tue, 02 Aug 2022 17:04:26: 33000000 INFO @ Tue, 02 Aug 2022 17:04:28: 43000000 INFO @ Tue, 02 Aug 2022 17:04:30: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:04:33: 34000000 INFO @ Tue, 02 Aug 2022 17:04:34: 44000000 INFO @ Tue, 02 Aug 2022 17:04:36: 41000000 INFO @ Tue, 02 Aug 2022 17:04:39: 35000000 INFO @ Tue, 02 Aug 2022 17:04:41: 45000000 INFO @ Tue, 02 Aug 2022 17:04:42: 42000000 INFO @ Tue, 02 Aug 2022 17:04:46: 36000000 INFO @ Tue, 02 Aug 2022 17:04:47: 46000000 INFO @ Tue, 02 Aug 2022 17:04:48: 43000000 INFO @ Tue, 02 Aug 2022 17:04:52: 37000000 INFO @ Tue, 02 Aug 2022 17:04:54: 47000000 INFO @ Tue, 02 Aug 2022 17:04:55: 44000000 INFO @ Tue, 02 Aug 2022 17:04:58: 38000000 INFO @ Tue, 02 Aug 2022 17:05:00: 48000000 INFO @ Tue, 02 Aug 2022 17:05:01: 45000000 INFO @ Tue, 02 Aug 2022 17:05:05: 39000000 INFO @ Tue, 02 Aug 2022 17:05:06: 49000000 INFO @ Tue, 02 Aug 2022 17:05:07: 46000000 INFO @ Tue, 02 Aug 2022 17:05:11: 40000000 INFO @ Tue, 02 Aug 2022 17:05:13: 50000000 INFO @ Tue, 02 Aug 2022 17:05:13: 47000000 INFO @ Tue, 02 Aug 2022 17:05:18: 41000000 INFO @ Tue, 02 Aug 2022 17:05:19: 48000000 INFO @ Tue, 02 Aug 2022 17:05:19: 51000000 INFO @ Tue, 02 Aug 2022 17:05:24: 42000000 INFO @ Tue, 02 Aug 2022 17:05:25: 49000000 INFO @ Tue, 02 Aug 2022 17:05:25: 52000000 INFO @ Tue, 02 Aug 2022 17:05:31: 43000000 INFO @ Tue, 02 Aug 2022 17:05:31: 50000000 INFO @ Tue, 02 Aug 2022 17:05:32: 53000000 INFO @ Tue, 02 Aug 2022 17:05:37: 51000000 INFO @ Tue, 02 Aug 2022 17:05:37: 44000000 INFO @ Tue, 02 Aug 2022 17:05:38: 54000000 INFO @ Tue, 02 Aug 2022 17:05:43: 52000000 INFO @ Tue, 02 Aug 2022 17:05:44: 45000000 INFO @ Tue, 02 Aug 2022 17:05:45: 55000000 INFO @ Tue, 02 Aug 2022 17:05:49: 53000000 INFO @ Tue, 02 Aug 2022 17:05:50: 46000000 INFO @ Tue, 02 Aug 2022 17:05:51: 56000000 INFO @ Tue, 02 Aug 2022 17:05:55: 54000000 INFO @ Tue, 02 Aug 2022 17:05:57: 47000000 INFO @ Tue, 02 Aug 2022 17:05:57: 57000000 INFO @ Tue, 02 Aug 2022 17:06:01: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:06:01: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:06:01: #1 total tags in treatment: 57481742 INFO @ Tue, 02 Aug 2022 17:06:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:06:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:06:01: 55000000 INFO @ Tue, 02 Aug 2022 17:06:02: #1 tags after filtering in treatment: 57481742 INFO @ Tue, 02 Aug 2022 17:06:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:06:02: #1 finished! INFO @ Tue, 02 Aug 2022 17:06:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:06:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:06:03: 48000000 INFO @ Tue, 02 Aug 2022 17:06:05: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:06:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:06:05: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 17:06:08: 56000000 cut: /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:06:10: 49000000 INFO @ Tue, 02 Aug 2022 17:06:14: 57000000 INFO @ Tue, 02 Aug 2022 17:06:16: 50000000 INFO @ Tue, 02 Aug 2022 17:06:18: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:06:18: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:06:18: #1 total tags in treatment: 57481742 INFO @ Tue, 02 Aug 2022 17:06:18: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:06:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:06:19: #1 tags after filtering in treatment: 57481742 INFO @ Tue, 02 Aug 2022 17:06:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:06:19: #1 finished! INFO @ Tue, 02 Aug 2022 17:06:19: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:06:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:06:22: 51000000 INFO @ Tue, 02 Aug 2022 17:06:23: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:06:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:06:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:06:28: 52000000 INFO @ Tue, 02 Aug 2022 17:06:33: 53000000 INFO @ Tue, 02 Aug 2022 17:06:39: 54000000 INFO @ Tue, 02 Aug 2022 17:06:45: 55000000 INFO @ Tue, 02 Aug 2022 17:06:51: 56000000 INFO @ Tue, 02 Aug 2022 17:06:56: 57000000 INFO @ Tue, 02 Aug 2022 17:06:59: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:06:59: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:06:59: #1 total tags in treatment: 57481742 INFO @ Tue, 02 Aug 2022 17:06:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:06:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:07:00: #1 tags after filtering in treatment: 57481742 INFO @ Tue, 02 Aug 2022 17:07:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:07:00: #1 finished! INFO @ Tue, 02 Aug 2022 17:07:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:07:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:07:04: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 17:07:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:07:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369899/SRX8369899.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling