Job ID = 14170531 SRX = SRX8174958 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 68573462 spots for SRR11608686/SRR11608686.sra Written 68573462 spots for SRR11608686/SRR11608686.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171018 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:22 68573462 reads; of these: 68573462 (100.00%) were unpaired; of these: 5609363 (8.18%) aligned 0 times 54531600 (79.52%) aligned exactly 1 time 8432499 (12.30%) aligned >1 times 91.82% overall alignment rate Time searching: 00:24:22 Overall time: 00:24:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13017831 / 62964099 = 0.2068 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:49:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:49:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:49:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:49:29: 1000000 INFO @ Sat, 11 Dec 2021 07:49:37: 2000000 INFO @ Sat, 11 Dec 2021 07:49:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:49:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:49:52: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:49:52: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:49:53: 4000000 INFO @ Sat, 11 Dec 2021 07:50:00: 1000000 INFO @ Sat, 11 Dec 2021 07:50:02: 5000000 INFO @ Sat, 11 Dec 2021 07:50:10: 2000000 INFO @ Sat, 11 Dec 2021 07:50:11: 6000000 INFO @ Sat, 11 Dec 2021 07:50:19: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:50:20: 7000000 INFO @ Sat, 11 Dec 2021 07:50:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:50:22: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:50:22: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:50:29: 4000000 INFO @ Sat, 11 Dec 2021 07:50:30: 8000000 INFO @ Sat, 11 Dec 2021 07:50:30: 1000000 INFO @ Sat, 11 Dec 2021 07:50:38: 5000000 INFO @ Sat, 11 Dec 2021 07:50:39: 2000000 INFO @ Sat, 11 Dec 2021 07:50:39: 9000000 INFO @ Sat, 11 Dec 2021 07:50:48: 6000000 INFO @ Sat, 11 Dec 2021 07:50:48: 3000000 INFO @ Sat, 11 Dec 2021 07:50:49: 10000000 INFO @ Sat, 11 Dec 2021 07:50:57: 4000000 INFO @ Sat, 11 Dec 2021 07:50:57: 7000000 INFO @ Sat, 11 Dec 2021 07:50:58: 11000000 INFO @ Sat, 11 Dec 2021 07:51:05: 5000000 INFO @ Sat, 11 Dec 2021 07:51:07: 8000000 INFO @ Sat, 11 Dec 2021 07:51:08: 12000000 INFO @ Sat, 11 Dec 2021 07:51:14: 6000000 INFO @ Sat, 11 Dec 2021 07:51:16: 9000000 INFO @ Sat, 11 Dec 2021 07:51:17: 13000000 INFO @ Sat, 11 Dec 2021 07:51:23: 7000000 INFO @ Sat, 11 Dec 2021 07:51:26: 10000000 INFO @ Sat, 11 Dec 2021 07:51:27: 14000000 INFO @ Sat, 11 Dec 2021 07:51:31: 8000000 INFO @ Sat, 11 Dec 2021 07:51:36: 11000000 INFO @ Sat, 11 Dec 2021 07:51:36: 15000000 INFO @ Sat, 11 Dec 2021 07:51:40: 9000000 INFO @ Sat, 11 Dec 2021 07:51:45: 16000000 INFO @ Sat, 11 Dec 2021 07:51:45: 12000000 INFO @ Sat, 11 Dec 2021 07:51:49: 10000000 INFO @ Sat, 11 Dec 2021 07:51:55: 17000000 INFO @ Sat, 11 Dec 2021 07:51:55: 13000000 INFO @ Sat, 11 Dec 2021 07:51:58: 11000000 INFO @ Sat, 11 Dec 2021 07:52:04: 18000000 INFO @ Sat, 11 Dec 2021 07:52:05: 14000000 INFO @ Sat, 11 Dec 2021 07:52:06: 12000000 INFO @ Sat, 11 Dec 2021 07:52:13: 19000000 INFO @ Sat, 11 Dec 2021 07:52:14: 15000000 INFO @ Sat, 11 Dec 2021 07:52:15: 13000000 INFO @ Sat, 11 Dec 2021 07:52:23: 20000000 INFO @ Sat, 11 Dec 2021 07:52:24: 14000000 INFO @ Sat, 11 Dec 2021 07:52:24: 16000000 INFO @ Sat, 11 Dec 2021 07:52:32: 21000000 INFO @ Sat, 11 Dec 2021 07:52:33: 15000000 INFO @ Sat, 11 Dec 2021 07:52:33: 17000000 INFO @ Sat, 11 Dec 2021 07:52:41: 22000000 INFO @ Sat, 11 Dec 2021 07:52:41: 16000000 INFO @ Sat, 11 Dec 2021 07:52:43: 18000000 INFO @ Sat, 11 Dec 2021 07:52:50: 17000000 INFO @ Sat, 11 Dec 2021 07:52:50: 23000000 INFO @ Sat, 11 Dec 2021 07:52:53: 19000000 INFO @ Sat, 11 Dec 2021 07:52:59: 18000000 INFO @ Sat, 11 Dec 2021 07:53:00: 24000000 INFO @ Sat, 11 Dec 2021 07:53:03: 20000000 INFO @ Sat, 11 Dec 2021 07:53:08: 19000000 INFO @ Sat, 11 Dec 2021 07:53:09: 25000000 INFO @ Sat, 11 Dec 2021 07:53:12: 21000000 INFO @ Sat, 11 Dec 2021 07:53:16: 20000000 INFO @ Sat, 11 Dec 2021 07:53:18: 26000000 INFO @ Sat, 11 Dec 2021 07:53:21: 22000000 INFO @ Sat, 11 Dec 2021 07:53:25: 21000000 INFO @ Sat, 11 Dec 2021 07:53:27: 27000000 INFO @ Sat, 11 Dec 2021 07:53:31: 23000000 INFO @ Sat, 11 Dec 2021 07:53:34: 22000000 INFO @ Sat, 11 Dec 2021 07:53:36: 28000000 INFO @ Sat, 11 Dec 2021 07:53:40: 24000000 INFO @ Sat, 11 Dec 2021 07:53:42: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:53:46: 29000000 INFO @ Sat, 11 Dec 2021 07:53:50: 25000000 INFO @ Sat, 11 Dec 2021 07:53:51: 24000000 INFO @ Sat, 11 Dec 2021 07:53:55: 30000000 INFO @ Sat, 11 Dec 2021 07:53:59: 26000000 INFO @ Sat, 11 Dec 2021 07:54:00: 25000000 INFO @ Sat, 11 Dec 2021 07:54:04: 31000000 INFO @ Sat, 11 Dec 2021 07:54:08: 26000000 INFO @ Sat, 11 Dec 2021 07:54:09: 27000000 INFO @ Sat, 11 Dec 2021 07:54:13: 32000000 INFO @ Sat, 11 Dec 2021 07:54:17: 27000000 INFO @ Sat, 11 Dec 2021 07:54:18: 28000000 INFO @ Sat, 11 Dec 2021 07:54:22: 33000000 INFO @ Sat, 11 Dec 2021 07:54:26: 28000000 INFO @ Sat, 11 Dec 2021 07:54:28: 29000000 INFO @ Sat, 11 Dec 2021 07:54:32: 34000000 INFO @ Sat, 11 Dec 2021 07:54:34: 29000000 INFO @ Sat, 11 Dec 2021 07:54:37: 30000000 INFO @ Sat, 11 Dec 2021 07:54:41: 35000000 INFO @ Sat, 11 Dec 2021 07:54:43: 30000000 INFO @ Sat, 11 Dec 2021 07:54:47: 31000000 INFO @ Sat, 11 Dec 2021 07:54:50: 36000000 INFO @ Sat, 11 Dec 2021 07:54:52: 31000000 INFO @ Sat, 11 Dec 2021 07:54:56: 32000000 INFO @ Sat, 11 Dec 2021 07:55:00: 37000000 INFO @ Sat, 11 Dec 2021 07:55:00: 32000000 INFO @ Sat, 11 Dec 2021 07:55:06: 33000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 07:55:09: 33000000 INFO @ Sat, 11 Dec 2021 07:55:09: 38000000 INFO @ Sat, 11 Dec 2021 07:55:15: 34000000 INFO @ Sat, 11 Dec 2021 07:55:18: 34000000 INFO @ Sat, 11 Dec 2021 07:55:19: 39000000 INFO @ Sat, 11 Dec 2021 07:55:25: 35000000 INFO @ Sat, 11 Dec 2021 07:55:27: 35000000 INFO @ Sat, 11 Dec 2021 07:55:28: 40000000 INFO @ Sat, 11 Dec 2021 07:55:34: 36000000 INFO @ Sat, 11 Dec 2021 07:55:35: 36000000 INFO @ Sat, 11 Dec 2021 07:55:37: 41000000 INFO @ Sat, 11 Dec 2021 07:55:44: 37000000 INFO @ Sat, 11 Dec 2021 07:55:45: 37000000 INFO @ Sat, 11 Dec 2021 07:55:47: 42000000 INFO @ Sat, 11 Dec 2021 07:55:53: 38000000 INFO @ Sat, 11 Dec 2021 07:55:54: 38000000 INFO @ Sat, 11 Dec 2021 07:55:56: 43000000 INFO @ Sat, 11 Dec 2021 07:56:03: 39000000 INFO @ Sat, 11 Dec 2021 07:56:04: 39000000 INFO @ Sat, 11 Dec 2021 07:56:05: 44000000 INFO @ Sat, 11 Dec 2021 07:56:13: 40000000 INFO @ Sat, 11 Dec 2021 07:56:14: 40000000 INFO @ Sat, 11 Dec 2021 07:56:15: 45000000 INFO @ Sat, 11 Dec 2021 07:56:23: 41000000 INFO @ Sat, 11 Dec 2021 07:56:24: 41000000 INFO @ Sat, 11 Dec 2021 07:56:24: 46000000 INFO @ Sat, 11 Dec 2021 07:56:33: 42000000 INFO @ Sat, 11 Dec 2021 07:56:33: 42000000 INFO @ Sat, 11 Dec 2021 07:56:34: 47000000 INFO @ Sat, 11 Dec 2021 07:56:42: 43000000 INFO @ Sat, 11 Dec 2021 07:56:43: 43000000 INFO @ Sat, 11 Dec 2021 07:56:44: 48000000 INFO @ Sat, 11 Dec 2021 07:56:51: 44000000 INFO @ Sat, 11 Dec 2021 07:56:52: 44000000 INFO @ Sat, 11 Dec 2021 07:56:53: 49000000 INFO @ Sat, 11 Dec 2021 07:57:01: 45000000 INFO @ Sat, 11 Dec 2021 07:57:02: 45000000 INFO @ Sat, 11 Dec 2021 07:57:02: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 07:57:02: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 07:57:02: #1 total tags in treatment: 49946268 INFO @ Sat, 11 Dec 2021 07:57:02: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:57:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:57:03: #1 tags after filtering in treatment: 49946268 INFO @ Sat, 11 Dec 2021 07:57:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:57:03: #1 finished! INFO @ Sat, 11 Dec 2021 07:57:03: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:57:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:57:06: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 07:57:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:57:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:57:10: 46000000 INFO @ Sat, 11 Dec 2021 07:57:11: 46000000 INFO @ Sat, 11 Dec 2021 07:57:20: 47000000 INFO @ Sat, 11 Dec 2021 07:57:21: 47000000 INFO @ Sat, 11 Dec 2021 07:57:30: 48000000 INFO @ Sat, 11 Dec 2021 07:57:31: 48000000 INFO @ Sat, 11 Dec 2021 07:57:40: 49000000 INFO @ Sat, 11 Dec 2021 07:57:41: 49000000 INFO @ Sat, 11 Dec 2021 07:57:49: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 07:57:49: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 07:57:49: #1 total tags in treatment: 49946268 INFO @ Sat, 11 Dec 2021 07:57:49: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:57:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:57:50: #1 tags after filtering in treatment: 49946268 INFO @ Sat, 11 Dec 2021 07:57:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:57:50: #1 finished! INFO @ Sat, 11 Dec 2021 07:57:50: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:57:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:57:50: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 07:57:50: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 07:57:50: #1 total tags in treatment: 49946268 INFO @ Sat, 11 Dec 2021 07:57:50: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:57:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:57:51: #1 tags after filtering in treatment: 49946268 INFO @ Sat, 11 Dec 2021 07:57:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:57:51: #1 finished! INFO @ Sat, 11 Dec 2021 07:57:51: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:57:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:57:53: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 07:57:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:57:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:57:54: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 07:57:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 07:57:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174958/SRX8174958.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling