Job ID = 12266676 SRX = SRX8174038 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26458906 spots for SRR11607676/SRR11607676.sra Written 26458906 spots for SRR11607676/SRR11607676.sra Read 12041128 spots for SRR11607677/SRR11607677.sra Written 12041128 spots for SRR11607677/SRR11607677.sra fastq に変換しました。 bowtie でマッピング中... Your job 12267664 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:58:13 38500034 reads; of these: 38500034 (100.00%) were paired; of these: 3852160 (10.01%) aligned concordantly 0 times 18748769 (48.70%) aligned concordantly exactly 1 time 15899105 (41.30%) aligned concordantly >1 times ---- 3852160 pairs aligned concordantly 0 times; of these: 1235620 (32.08%) aligned discordantly 1 time ---- 2616540 pairs aligned 0 times concordantly or discordantly; of these: 5233080 mates make up the pairs; of these: 2928181 (55.96%) aligned 0 times 775225 (14.81%) aligned exactly 1 time 1529674 (29.23%) aligned >1 times 96.20% overall alignment rate Time searching: 00:58:13 Overall time: 00:58:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10887074 / 35509674 = 0.3066 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:25:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:25:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:25:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:25:39: 1000000 INFO @ Sat, 03 Apr 2021 10:25:45: 2000000 INFO @ Sat, 03 Apr 2021 10:25:52: 3000000 INFO @ Sat, 03 Apr 2021 10:25:58: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:26:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:26:02: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:26:02: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:26:05: 5000000 INFO @ Sat, 03 Apr 2021 10:26:12: 1000000 INFO @ Sat, 03 Apr 2021 10:26:12: 6000000 INFO @ Sat, 03 Apr 2021 10:26:19: 7000000 INFO @ Sat, 03 Apr 2021 10:26:22: 2000000 INFO @ Sat, 03 Apr 2021 10:26:26: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:26:32: 3000000 INFO @ Sat, 03 Apr 2021 10:26:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:26:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:26:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:26:33: 9000000 INFO @ Sat, 03 Apr 2021 10:26:40: 10000000 INFO @ Sat, 03 Apr 2021 10:26:40: 1000000 INFO @ Sat, 03 Apr 2021 10:26:42: 4000000 INFO @ Sat, 03 Apr 2021 10:26:47: 11000000 INFO @ Sat, 03 Apr 2021 10:26:48: 2000000 INFO @ Sat, 03 Apr 2021 10:26:52: 5000000 INFO @ Sat, 03 Apr 2021 10:26:54: 12000000 INFO @ Sat, 03 Apr 2021 10:26:56: 3000000 INFO @ Sat, 03 Apr 2021 10:27:01: 13000000 INFO @ Sat, 03 Apr 2021 10:27:02: 6000000 INFO @ Sat, 03 Apr 2021 10:27:04: 4000000 INFO @ Sat, 03 Apr 2021 10:27:07: 14000000 INFO @ Sat, 03 Apr 2021 10:27:12: 7000000 INFO @ Sat, 03 Apr 2021 10:27:12: 5000000 INFO @ Sat, 03 Apr 2021 10:27:14: 15000000 INFO @ Sat, 03 Apr 2021 10:27:21: 6000000 INFO @ Sat, 03 Apr 2021 10:27:21: 16000000 INFO @ Sat, 03 Apr 2021 10:27:23: 8000000 INFO @ Sat, 03 Apr 2021 10:27:28: 17000000 INFO @ Sat, 03 Apr 2021 10:27:29: 7000000 INFO @ Sat, 03 Apr 2021 10:27:33: 9000000 INFO @ Sat, 03 Apr 2021 10:27:35: 18000000 INFO @ Sat, 03 Apr 2021 10:27:37: 8000000 INFO @ Sat, 03 Apr 2021 10:27:42: 19000000 INFO @ Sat, 03 Apr 2021 10:27:43: 10000000 INFO @ Sat, 03 Apr 2021 10:27:45: 9000000 INFO @ Sat, 03 Apr 2021 10:27:49: 20000000 INFO @ Sat, 03 Apr 2021 10:27:53: 11000000 INFO @ Sat, 03 Apr 2021 10:27:54: 10000000 INFO @ Sat, 03 Apr 2021 10:27:56: 21000000 INFO @ Sat, 03 Apr 2021 10:28:02: 11000000 INFO @ Sat, 03 Apr 2021 10:28:03: 22000000 INFO @ Sat, 03 Apr 2021 10:28:03: 12000000 INFO @ Sat, 03 Apr 2021 10:28:10: 23000000 INFO @ Sat, 03 Apr 2021 10:28:10: 12000000 INFO @ Sat, 03 Apr 2021 10:28:13: 13000000 INFO @ Sat, 03 Apr 2021 10:28:17: 24000000 INFO @ Sat, 03 Apr 2021 10:28:19: 13000000 INFO @ Sat, 03 Apr 2021 10:28:23: 14000000 INFO @ Sat, 03 Apr 2021 10:28:24: 25000000 INFO @ Sat, 03 Apr 2021 10:28:27: 14000000 INFO @ Sat, 03 Apr 2021 10:28:31: 26000000 INFO @ Sat, 03 Apr 2021 10:28:33: 15000000 INFO @ Sat, 03 Apr 2021 10:28:35: 15000000 INFO @ Sat, 03 Apr 2021 10:28:38: 27000000 INFO @ Sat, 03 Apr 2021 10:28:43: 16000000 INFO @ Sat, 03 Apr 2021 10:28:44: 16000000 INFO @ Sat, 03 Apr 2021 10:28:46: 28000000 INFO @ Sat, 03 Apr 2021 10:28:53: 17000000 INFO @ Sat, 03 Apr 2021 10:28:54: 17000000 INFO @ Sat, 03 Apr 2021 10:28:56: 29000000 INFO @ Sat, 03 Apr 2021 10:29:01: 18000000 INFO @ Sat, 03 Apr 2021 10:29:05: 18000000 INFO @ Sat, 03 Apr 2021 10:29:08: 30000000 INFO @ Sat, 03 Apr 2021 10:29:09: 19000000 INFO @ Sat, 03 Apr 2021 10:29:16: 19000000 INFO @ Sat, 03 Apr 2021 10:29:17: 20000000 INFO @ Sat, 03 Apr 2021 10:29:19: 31000000 INFO @ Sat, 03 Apr 2021 10:29:25: 21000000 INFO @ Sat, 03 Apr 2021 10:29:27: 20000000 INFO @ Sat, 03 Apr 2021 10:29:31: 32000000 INFO @ Sat, 03 Apr 2021 10:29:33: 22000000 INFO @ Sat, 03 Apr 2021 10:29:38: 21000000 INFO @ Sat, 03 Apr 2021 10:29:41: 23000000 INFO @ Sat, 03 Apr 2021 10:29:42: 33000000 INFO @ Sat, 03 Apr 2021 10:29:49: 22000000 INFO @ Sat, 03 Apr 2021 10:29:49: 24000000 INFO @ Sat, 03 Apr 2021 10:29:54: 34000000 INFO @ Sat, 03 Apr 2021 10:29:57: 25000000 INFO @ Sat, 03 Apr 2021 10:29:59: 23000000 INFO @ Sat, 03 Apr 2021 10:30:05: 35000000 INFO @ Sat, 03 Apr 2021 10:30:05: 26000000 INFO @ Sat, 03 Apr 2021 10:30:10: 24000000 INFO @ Sat, 03 Apr 2021 10:30:13: 27000000 INFO @ Sat, 03 Apr 2021 10:30:16: 36000000 INFO @ Sat, 03 Apr 2021 10:30:21: 25000000 INFO @ Sat, 03 Apr 2021 10:30:21: 28000000 INFO @ Sat, 03 Apr 2021 10:30:28: 37000000 INFO @ Sat, 03 Apr 2021 10:30:29: 29000000 INFO @ Sat, 03 Apr 2021 10:30:32: 26000000 INFO @ Sat, 03 Apr 2021 10:30:37: 30000000 INFO @ Sat, 03 Apr 2021 10:30:40: 38000000 INFO @ Sat, 03 Apr 2021 10:30:43: 27000000 INFO @ Sat, 03 Apr 2021 10:30:46: 31000000 INFO @ Sat, 03 Apr 2021 10:30:50: 39000000 INFO @ Sat, 03 Apr 2021 10:30:54: 32000000 INFO @ Sat, 03 Apr 2021 10:30:54: 28000000 INFO @ Sat, 03 Apr 2021 10:31:01: 40000000 INFO @ Sat, 03 Apr 2021 10:31:02: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 10:31:05: 29000000 INFO @ Sat, 03 Apr 2021 10:31:10: 34000000 INFO @ Sat, 03 Apr 2021 10:31:12: 41000000 INFO @ Sat, 03 Apr 2021 10:31:16: 30000000 INFO @ Sat, 03 Apr 2021 10:31:18: 35000000 INFO @ Sat, 03 Apr 2021 10:31:23: 42000000 INFO @ Sat, 03 Apr 2021 10:31:26: 36000000 INFO @ Sat, 03 Apr 2021 10:31:27: 31000000 INFO @ Sat, 03 Apr 2021 10:31:34: 37000000 INFO @ Sat, 03 Apr 2021 10:31:34: 43000000 INFO @ Sat, 03 Apr 2021 10:31:38: 32000000 INFO @ Sat, 03 Apr 2021 10:31:42: 38000000 INFO @ Sat, 03 Apr 2021 10:31:46: 44000000 INFO @ Sat, 03 Apr 2021 10:31:49: 33000000 INFO @ Sat, 03 Apr 2021 10:31:50: 39000000 INFO @ Sat, 03 Apr 2021 10:31:57: 45000000 INFO @ Sat, 03 Apr 2021 10:31:58: 40000000 INFO @ Sat, 03 Apr 2021 10:32:01: 34000000 INFO @ Sat, 03 Apr 2021 10:32:05: 41000000 INFO @ Sat, 03 Apr 2021 10:32:08: 46000000 INFO @ Sat, 03 Apr 2021 10:32:11: 35000000 INFO @ Sat, 03 Apr 2021 10:32:13: 42000000 INFO @ Sat, 03 Apr 2021 10:32:19: 47000000 INFO @ Sat, 03 Apr 2021 10:32:21: 43000000 INFO @ Sat, 03 Apr 2021 10:32:22: 36000000 INFO @ Sat, 03 Apr 2021 10:32:29: 44000000 INFO @ Sat, 03 Apr 2021 10:32:30: 48000000 INFO @ Sat, 03 Apr 2021 10:32:33: 37000000 INFO @ Sat, 03 Apr 2021 10:32:37: 45000000 INFO @ Sat, 03 Apr 2021 10:32:41: 49000000 INFO @ Sat, 03 Apr 2021 10:32:44: 38000000 INFO @ Sat, 03 Apr 2021 10:32:45: 46000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 10:32:52: 50000000 INFO @ Sat, 03 Apr 2021 10:32:53: 47000000 INFO @ Sat, 03 Apr 2021 10:32:55: 39000000 INFO @ Sat, 03 Apr 2021 10:33:01: 48000000 INFO @ Sat, 03 Apr 2021 10:33:03: 51000000 INFO @ Sat, 03 Apr 2021 10:33:06: 40000000 INFO @ Sat, 03 Apr 2021 10:33:09: 49000000 INFO @ Sat, 03 Apr 2021 10:33:13: 52000000 INFO @ Sat, 03 Apr 2021 10:33:16: 41000000 INFO @ Sat, 03 Apr 2021 10:33:17: #1 tag size is determined as 38 bps INFO @ Sat, 03 Apr 2021 10:33:17: #1 tag size = 38 INFO @ Sat, 03 Apr 2021 10:33:17: #1 total tags in treatment: 23819354 INFO @ Sat, 03 Apr 2021 10:33:17: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:33:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:33:17: 50000000 INFO @ Sat, 03 Apr 2021 10:33:17: #1 tags after filtering in treatment: 17722427 INFO @ Sat, 03 Apr 2021 10:33:17: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 03 Apr 2021 10:33:17: #1 finished! INFO @ Sat, 03 Apr 2021 10:33:17: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:33:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:33:19: #2 number of paired peaks: 293 WARNING @ Sat, 03 Apr 2021 10:33:19: Fewer paired peaks (293) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 293 pairs to build model! INFO @ Sat, 03 Apr 2021 10:33:19: start model_add_line... INFO @ Sat, 03 Apr 2021 10:33:19: start X-correlation... INFO @ Sat, 03 Apr 2021 10:33:19: end of X-cor INFO @ Sat, 03 Apr 2021 10:33:19: #2 finished! INFO @ Sat, 03 Apr 2021 10:33:19: #2 predicted fragment length is 51 bps INFO @ Sat, 03 Apr 2021 10:33:19: #2 alternative fragment length(s) may be 2,44,51 bps INFO @ Sat, 03 Apr 2021 10:33:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.05_model.r WARNING @ Sat, 03 Apr 2021 10:33:19: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:33:19: #2 You may need to consider one of the other alternative d(s): 2,44,51 WARNING @ Sat, 03 Apr 2021 10:33:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:33:19: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:33:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:33:25: 51000000 INFO @ Sat, 03 Apr 2021 10:33:26: 42000000 INFO @ Sat, 03 Apr 2021 10:33:32: 52000000 INFO @ Sat, 03 Apr 2021 10:33:35: #1 tag size is determined as 38 bps INFO @ Sat, 03 Apr 2021 10:33:35: #1 tag size = 38 INFO @ Sat, 03 Apr 2021 10:33:35: #1 total tags in treatment: 23819354 INFO @ Sat, 03 Apr 2021 10:33:35: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:33:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:33:35: 43000000 INFO @ Sat, 03 Apr 2021 10:33:35: #1 tags after filtering in treatment: 17722427 INFO @ Sat, 03 Apr 2021 10:33:35: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 03 Apr 2021 10:33:35: #1 finished! INFO @ Sat, 03 Apr 2021 10:33:35: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:33:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:33:37: #2 number of paired peaks: 293 WARNING @ Sat, 03 Apr 2021 10:33:37: Fewer paired peaks (293) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 293 pairs to build model! INFO @ Sat, 03 Apr 2021 10:33:37: start model_add_line... INFO @ Sat, 03 Apr 2021 10:33:37: start X-correlation... INFO @ Sat, 03 Apr 2021 10:33:37: end of X-cor INFO @ Sat, 03 Apr 2021 10:33:37: #2 finished! INFO @ Sat, 03 Apr 2021 10:33:37: #2 predicted fragment length is 51 bps INFO @ Sat, 03 Apr 2021 10:33:37: #2 alternative fragment length(s) may be 2,44,51 bps INFO @ Sat, 03 Apr 2021 10:33:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.20_model.r WARNING @ Sat, 03 Apr 2021 10:33:37: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:33:37: #2 You may need to consider one of the other alternative d(s): 2,44,51 WARNING @ Sat, 03 Apr 2021 10:33:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:33:37: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:33:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:33:45: 44000000 INFO @ Sat, 03 Apr 2021 10:33:54: 45000000 INFO @ Sat, 03 Apr 2021 10:34:03: 46000000 INFO @ Sat, 03 Apr 2021 10:34:05: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:34:13: 47000000 INFO @ Sat, 03 Apr 2021 10:34:22: 48000000 INFO @ Sat, 03 Apr 2021 10:34:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:34:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.05_peaks.xls INFO @ Sat, 03 Apr 2021 10:34:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:34:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.05_summits.bed INFO @ Sat, 03 Apr 2021 10:34:31: Done! INFO @ Sat, 03 Apr 2021 10:34:31: 49000000 pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (9242 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:34:40: 50000000 INFO @ Sat, 03 Apr 2021 10:34:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.20_peaks.xls INFO @ Sat, 03 Apr 2021 10:34:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:34:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.20_summits.bed INFO @ Sat, 03 Apr 2021 10:34:48: Done! pass1 - making usageList (8 chroms): 2 millis pass2 - checking and writing primary data (211 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:34:50: 51000000 INFO @ Sat, 03 Apr 2021 10:34:59: 52000000 INFO @ Sat, 03 Apr 2021 10:35:02: #1 tag size is determined as 38 bps INFO @ Sat, 03 Apr 2021 10:35:02: #1 tag size = 38 INFO @ Sat, 03 Apr 2021 10:35:02: #1 total tags in treatment: 23819354 INFO @ Sat, 03 Apr 2021 10:35:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:35:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:35:03: #1 tags after filtering in treatment: 17722427 INFO @ Sat, 03 Apr 2021 10:35:03: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 03 Apr 2021 10:35:03: #1 finished! INFO @ Sat, 03 Apr 2021 10:35:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:35:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:35:04: #2 number of paired peaks: 293 WARNING @ Sat, 03 Apr 2021 10:35:04: Fewer paired peaks (293) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 293 pairs to build model! INFO @ Sat, 03 Apr 2021 10:35:04: start model_add_line... INFO @ Sat, 03 Apr 2021 10:35:04: start X-correlation... INFO @ Sat, 03 Apr 2021 10:35:04: end of X-cor INFO @ Sat, 03 Apr 2021 10:35:04: #2 finished! INFO @ Sat, 03 Apr 2021 10:35:04: #2 predicted fragment length is 51 bps INFO @ Sat, 03 Apr 2021 10:35:04: #2 alternative fragment length(s) may be 2,44,51 bps INFO @ Sat, 03 Apr 2021 10:35:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.10_model.r WARNING @ Sat, 03 Apr 2021 10:35:04: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:35:04: #2 You may need to consider one of the other alternative d(s): 2,44,51 WARNING @ Sat, 03 Apr 2021 10:35:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:35:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:35:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:35:51: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:36:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.10_peaks.xls INFO @ Sat, 03 Apr 2021 10:36:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:36:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8174038/SRX8174038.10_summits.bed INFO @ Sat, 03 Apr 2021 10:36:16: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1948 records, 4 fields): 5 millis CompletedMACS2peakCalling