Job ID = 6627009 SRX = SRX8156262 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:19:34 prefetch.2.10.7: 1) Downloading 'SRR11588807'... 2020-07-14T00:19:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:21:45 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:21:45 prefetch.2.10.7: 1) 'SRR11588807' was downloaded successfully Read 19338448 spots for SRR11588807/SRR11588807.sra Written 19338448 spots for SRR11588807/SRR11588807.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627158 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:07 19338448 reads; of these: 19338448 (100.00%) were unpaired; of these: 2227062 (11.52%) aligned 0 times 13354721 (69.06%) aligned exactly 1 time 3756665 (19.43%) aligned >1 times 88.48% overall alignment rate Time searching: 00:05:07 Overall time: 00:05:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3340867 / 17111386 = 0.1952 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:31:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:31:23: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:31:23: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:31:29: 1000000 INFO @ Tue, 14 Jul 2020 09:31:34: 2000000 INFO @ Tue, 14 Jul 2020 09:31:38: 3000000 INFO @ Tue, 14 Jul 2020 09:31:44: 4000000 INFO @ Tue, 14 Jul 2020 09:31:49: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:31:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:31:53: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:31:53: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:31:55: 6000000 INFO @ Tue, 14 Jul 2020 09:31:59: 1000000 INFO @ Tue, 14 Jul 2020 09:32:01: 7000000 INFO @ Tue, 14 Jul 2020 09:32:05: 2000000 INFO @ Tue, 14 Jul 2020 09:32:07: 8000000 INFO @ Tue, 14 Jul 2020 09:32:11: 3000000 INFO @ Tue, 14 Jul 2020 09:32:12: 9000000 INFO @ Tue, 14 Jul 2020 09:32:17: 4000000 INFO @ Tue, 14 Jul 2020 09:32:18: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:32:23: 5000000 INFO @ Tue, 14 Jul 2020 09:32:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:32:23: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:32:23: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:32:24: 11000000 INFO @ Tue, 14 Jul 2020 09:32:29: 6000000 INFO @ Tue, 14 Jul 2020 09:32:29: 1000000 INFO @ Tue, 14 Jul 2020 09:32:30: 12000000 INFO @ Tue, 14 Jul 2020 09:32:35: 7000000 INFO @ Tue, 14 Jul 2020 09:32:35: 2000000 INFO @ Tue, 14 Jul 2020 09:32:35: 13000000 INFO @ Tue, 14 Jul 2020 09:32:40: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:32:40: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:32:40: #1 total tags in treatment: 13770519 INFO @ Tue, 14 Jul 2020 09:32:40: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:32:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:32:40: #1 tags after filtering in treatment: 13770519 INFO @ Tue, 14 Jul 2020 09:32:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:32:40: #1 finished! INFO @ Tue, 14 Jul 2020 09:32:40: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:32:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:32:41: 8000000 INFO @ Tue, 14 Jul 2020 09:32:41: 3000000 INFO @ Tue, 14 Jul 2020 09:32:41: #2 number of paired peaks: 63 WARNING @ Tue, 14 Jul 2020 09:32:41: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:32:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:32:46: 9000000 INFO @ Tue, 14 Jul 2020 09:32:47: 4000000 INFO @ Tue, 14 Jul 2020 09:32:52: 10000000 INFO @ Tue, 14 Jul 2020 09:32:53: 5000000 INFO @ Tue, 14 Jul 2020 09:32:58: 11000000 INFO @ Tue, 14 Jul 2020 09:32:58: 6000000 INFO @ Tue, 14 Jul 2020 09:33:03: 12000000 INFO @ Tue, 14 Jul 2020 09:33:04: 7000000 INFO @ Tue, 14 Jul 2020 09:33:09: 13000000 INFO @ Tue, 14 Jul 2020 09:33:10: 8000000 INFO @ Tue, 14 Jul 2020 09:33:14: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:33:14: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:33:14: #1 total tags in treatment: 13770519 INFO @ Tue, 14 Jul 2020 09:33:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:33:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:33:14: #1 tags after filtering in treatment: 13770519 INFO @ Tue, 14 Jul 2020 09:33:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:33:14: #1 finished! INFO @ Tue, 14 Jul 2020 09:33:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:33:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:33:15: #2 number of paired peaks: 63 WARNING @ Tue, 14 Jul 2020 09:33:15: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:33:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:33:15: 9000000 INFO @ Tue, 14 Jul 2020 09:33:21: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:33:27: 11000000 INFO @ Tue, 14 Jul 2020 09:33:32: 12000000 INFO @ Tue, 14 Jul 2020 09:33:38: 13000000 INFO @ Tue, 14 Jul 2020 09:33:42: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:33:42: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:33:42: #1 total tags in treatment: 13770519 INFO @ Tue, 14 Jul 2020 09:33:42: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:33:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:33:43: #1 tags after filtering in treatment: 13770519 INFO @ Tue, 14 Jul 2020 09:33:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:33:43: #1 finished! INFO @ Tue, 14 Jul 2020 09:33:43: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:33:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:33:44: #2 number of paired peaks: 63 WARNING @ Tue, 14 Jul 2020 09:33:44: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:33:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156262/SRX8156262.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。