Job ID = 6627007 SRX = SRX8156260 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:19:03 prefetch.2.10.7: 1) Downloading 'SRR11588805'... 2020-07-14T00:19:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:20:07 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:20:07 prefetch.2.10.7: 1) 'SRR11588805' was downloaded successfully Read 16458802 spots for SRR11588805/SRR11588805.sra Written 16458802 spots for SRR11588805/SRR11588805.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627154 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:12 16458802 reads; of these: 16458802 (100.00%) were unpaired; of these: 1045761 (6.35%) aligned 0 times 11648393 (70.77%) aligned exactly 1 time 3764648 (22.87%) aligned >1 times 93.65% overall alignment rate Time searching: 00:05:12 Overall time: 00:05:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2560363 / 15413041 = 0.1661 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:29:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:29:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:29:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:29:45: 1000000 INFO @ Tue, 14 Jul 2020 09:29:50: 2000000 INFO @ Tue, 14 Jul 2020 09:29:55: 3000000 INFO @ Tue, 14 Jul 2020 09:30:00: 4000000 INFO @ Tue, 14 Jul 2020 09:30:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:10: 6000000 INFO @ Tue, 14 Jul 2020 09:30:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:15: 7000000 INFO @ Tue, 14 Jul 2020 09:30:17: 1000000 INFO @ Tue, 14 Jul 2020 09:30:20: 8000000 INFO @ Tue, 14 Jul 2020 09:30:23: 2000000 INFO @ Tue, 14 Jul 2020 09:30:25: 9000000 INFO @ Tue, 14 Jul 2020 09:30:30: 3000000 INFO @ Tue, 14 Jul 2020 09:30:30: 10000000 INFO @ Tue, 14 Jul 2020 09:30:35: 11000000 INFO @ Tue, 14 Jul 2020 09:30:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:41: 12000000 INFO @ Tue, 14 Jul 2020 09:30:43: 5000000 INFO @ Tue, 14 Jul 2020 09:30:45: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:30:45: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:30:45: #1 total tags in treatment: 12852678 INFO @ Tue, 14 Jul 2020 09:30:45: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:30:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:30:45: #1 tags after filtering in treatment: 12852678 INFO @ Tue, 14 Jul 2020 09:30:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:30:45: #1 finished! INFO @ Tue, 14 Jul 2020 09:30:45: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:30:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:30:46: 1000000 INFO @ Tue, 14 Jul 2020 09:30:46: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 09:30:46: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:30:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:30:49: 6000000 INFO @ Tue, 14 Jul 2020 09:30:51: 2000000 INFO @ Tue, 14 Jul 2020 09:30:55: 7000000 INFO @ Tue, 14 Jul 2020 09:30:56: 3000000 INFO @ Tue, 14 Jul 2020 09:31:00: 4000000 INFO @ Tue, 14 Jul 2020 09:31:01: 8000000 INFO @ Tue, 14 Jul 2020 09:31:05: 5000000 INFO @ Tue, 14 Jul 2020 09:31:07: 9000000 INFO @ Tue, 14 Jul 2020 09:31:10: 6000000 INFO @ Tue, 14 Jul 2020 09:31:13: 10000000 INFO @ Tue, 14 Jul 2020 09:31:15: 7000000 INFO @ Tue, 14 Jul 2020 09:31:20: 11000000 INFO @ Tue, 14 Jul 2020 09:31:20: 8000000 INFO @ Tue, 14 Jul 2020 09:31:25: 9000000 INFO @ Tue, 14 Jul 2020 09:31:26: 12000000 INFO @ Tue, 14 Jul 2020 09:31:30: 10000000 INFO @ Tue, 14 Jul 2020 09:31:31: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:31:31: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:31:31: #1 total tags in treatment: 12852678 INFO @ Tue, 14 Jul 2020 09:31:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:31:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:31:31: #1 tags after filtering in treatment: 12852678 INFO @ Tue, 14 Jul 2020 09:31:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:31:31: #1 finished! INFO @ Tue, 14 Jul 2020 09:31:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:31:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:31:32: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 09:31:32: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:31:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:31:35: 11000000 INFO @ Tue, 14 Jul 2020 09:31:40: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:31:44: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:31:44: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:31:44: #1 total tags in treatment: 12852678 INFO @ Tue, 14 Jul 2020 09:31:44: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:31:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:31:44: #1 tags after filtering in treatment: 12852678 INFO @ Tue, 14 Jul 2020 09:31:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:31:44: #1 finished! INFO @ Tue, 14 Jul 2020 09:31:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:31:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:31:45: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 09:31:45: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:31:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156260/SRX8156260.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。