Job ID = 6528511 SRX = SRX815532 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:11:17 prefetch.2.10.7: 1) Downloading 'SRR1721320'... 2020-06-29T15:11:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:14:19 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:14:19 prefetch.2.10.7: 1) 'SRR1721320' was downloaded successfully Read 19002887 spots for SRR1721320/SRR1721320.sra Written 19002887 spots for SRR1721320/SRR1721320.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 19002887 reads; of these: 19002887 (100.00%) were unpaired; of these: 441700 (2.32%) aligned 0 times 13779062 (72.51%) aligned exactly 1 time 4782125 (25.17%) aligned >1 times 97.68% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1078500 / 18561187 = 0.0581 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:30:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:30:11: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:30:11: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:30:17: 1000000 INFO @ Tue, 30 Jun 2020 00:30:23: 2000000 INFO @ Tue, 30 Jun 2020 00:30:28: 3000000 INFO @ Tue, 30 Jun 2020 00:30:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:30:40: 5000000 INFO @ Tue, 30 Jun 2020 00:30:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:30:41: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:30:41: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:30:46: 6000000 INFO @ Tue, 30 Jun 2020 00:30:47: 1000000 INFO @ Tue, 30 Jun 2020 00:30:52: 7000000 INFO @ Tue, 30 Jun 2020 00:30:53: 2000000 INFO @ Tue, 30 Jun 2020 00:30:59: 8000000 INFO @ Tue, 30 Jun 2020 00:31:00: 3000000 INFO @ Tue, 30 Jun 2020 00:31:06: 9000000 INFO @ Tue, 30 Jun 2020 00:31:07: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:31:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:31:11: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:31:11: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:31:14: 10000000 INFO @ Tue, 30 Jun 2020 00:31:15: 5000000 INFO @ Tue, 30 Jun 2020 00:31:19: 1000000 INFO @ Tue, 30 Jun 2020 00:31:21: 11000000 INFO @ Tue, 30 Jun 2020 00:31:23: 6000000 INFO @ Tue, 30 Jun 2020 00:31:27: 2000000 INFO @ Tue, 30 Jun 2020 00:31:28: 12000000 INFO @ Tue, 30 Jun 2020 00:31:30: 7000000 INFO @ Tue, 30 Jun 2020 00:31:35: 3000000 INFO @ Tue, 30 Jun 2020 00:31:36: 13000000 INFO @ Tue, 30 Jun 2020 00:31:38: 8000000 INFO @ Tue, 30 Jun 2020 00:31:43: 4000000 INFO @ Tue, 30 Jun 2020 00:31:44: 14000000 INFO @ Tue, 30 Jun 2020 00:31:45: 9000000 INFO @ Tue, 30 Jun 2020 00:31:51: 15000000 INFO @ Tue, 30 Jun 2020 00:31:51: 5000000 INFO @ Tue, 30 Jun 2020 00:31:52: 10000000 INFO @ Tue, 30 Jun 2020 00:31:59: 16000000 INFO @ Tue, 30 Jun 2020 00:31:59: 6000000 INFO @ Tue, 30 Jun 2020 00:32:00: 11000000 INFO @ Tue, 30 Jun 2020 00:32:06: 17000000 INFO @ Tue, 30 Jun 2020 00:32:07: 7000000 INFO @ Tue, 30 Jun 2020 00:32:07: 12000000 INFO @ Tue, 30 Jun 2020 00:32:10: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:32:10: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:32:10: #1 total tags in treatment: 17482687 INFO @ Tue, 30 Jun 2020 00:32:10: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:32:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:32:10: #1 tags after filtering in treatment: 17482687 INFO @ Tue, 30 Jun 2020 00:32:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:32:10: #1 finished! INFO @ Tue, 30 Jun 2020 00:32:10: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:32:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:32:11: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:32:11: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:32:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:32:15: 8000000 INFO @ Tue, 30 Jun 2020 00:32:15: 13000000 INFO @ Tue, 30 Jun 2020 00:32:22: 9000000 INFO @ Tue, 30 Jun 2020 00:32:23: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:32:30: 10000000 INFO @ Tue, 30 Jun 2020 00:32:30: 15000000 INFO @ Tue, 30 Jun 2020 00:32:38: 16000000 INFO @ Tue, 30 Jun 2020 00:32:38: 11000000 INFO @ Tue, 30 Jun 2020 00:32:45: 17000000 INFO @ Tue, 30 Jun 2020 00:32:46: 12000000 INFO @ Tue, 30 Jun 2020 00:32:49: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:32:49: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:32:49: #1 total tags in treatment: 17482687 INFO @ Tue, 30 Jun 2020 00:32:49: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:32:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:32:49: #1 tags after filtering in treatment: 17482687 INFO @ Tue, 30 Jun 2020 00:32:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:32:49: #1 finished! INFO @ Tue, 30 Jun 2020 00:32:49: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:32:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:32:50: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:32:50: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:32:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:32:54: 13000000 INFO @ Tue, 30 Jun 2020 00:33:02: 14000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:33:09: 15000000 INFO @ Tue, 30 Jun 2020 00:33:17: 16000000 INFO @ Tue, 30 Jun 2020 00:33:25: 17000000 INFO @ Tue, 30 Jun 2020 00:33:28: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:33:28: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:33:28: #1 total tags in treatment: 17482687 INFO @ Tue, 30 Jun 2020 00:33:28: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:33:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:33:29: #1 tags after filtering in treatment: 17482687 INFO @ Tue, 30 Jun 2020 00:33:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:33:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:33:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:33:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:33:30: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:33:30: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:33:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815532/SRX815532.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling