Job ID = 14167283 SRX = SRX8076974 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21234358 spots for SRR11504846/SRR11504846.sra Written 21234358 spots for SRR11504846/SRR11504846.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167952 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:24 21234358 reads; of these: 21234358 (100.00%) were paired; of these: 12081315 (56.90%) aligned concordantly 0 times 7541089 (35.51%) aligned concordantly exactly 1 time 1611954 (7.59%) aligned concordantly >1 times ---- 12081315 pairs aligned concordantly 0 times; of these: 422559 (3.50%) aligned discordantly 1 time ---- 11658756 pairs aligned 0 times concordantly or discordantly; of these: 23317512 mates make up the pairs; of these: 22092141 (94.74%) aligned 0 times 801803 (3.44%) aligned exactly 1 time 423568 (1.82%) aligned >1 times 47.98% overall alignment rate Time searching: 00:17:25 Overall time: 00:17:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 706033 / 9541030 = 0.0740 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:57:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:57:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:57:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:57:58: 1000000 INFO @ Fri, 10 Dec 2021 11:58:03: 2000000 INFO @ Fri, 10 Dec 2021 11:58:08: 3000000 INFO @ Fri, 10 Dec 2021 11:58:12: 4000000 INFO @ Fri, 10 Dec 2021 11:58:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:58:22: 6000000 INFO @ Fri, 10 Dec 2021 11:58:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:58:23: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:58:23: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:58:28: 7000000 INFO @ Fri, 10 Dec 2021 11:58:29: 1000000 INFO @ Fri, 10 Dec 2021 11:58:33: 8000000 INFO @ Fri, 10 Dec 2021 11:58:36: 2000000 INFO @ Fri, 10 Dec 2021 11:58:39: 9000000 INFO @ Fri, 10 Dec 2021 11:58:42: 3000000 INFO @ Fri, 10 Dec 2021 11:58:45: 10000000 INFO @ Fri, 10 Dec 2021 11:58:48: 4000000 INFO @ Fri, 10 Dec 2021 11:58:50: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:58:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:58:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:58:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:58:54: 5000000 INFO @ Fri, 10 Dec 2021 11:58:56: 12000000 INFO @ Fri, 10 Dec 2021 11:58:58: 1000000 INFO @ Fri, 10 Dec 2021 11:59:01: 6000000 INFO @ Fri, 10 Dec 2021 11:59:01: 13000000 INFO @ Fri, 10 Dec 2021 11:59:04: 2000000 INFO @ Fri, 10 Dec 2021 11:59:06: 14000000 INFO @ Fri, 10 Dec 2021 11:59:07: 7000000 INFO @ Fri, 10 Dec 2021 11:59:09: 3000000 INFO @ Fri, 10 Dec 2021 11:59:12: 15000000 INFO @ Fri, 10 Dec 2021 11:59:13: 8000000 INFO @ Fri, 10 Dec 2021 11:59:15: 4000000 INFO @ Fri, 10 Dec 2021 11:59:17: 16000000 INFO @ Fri, 10 Dec 2021 11:59:19: 9000000 INFO @ Fri, 10 Dec 2021 11:59:20: 5000000 INFO @ Fri, 10 Dec 2021 11:59:23: 17000000 INFO @ Fri, 10 Dec 2021 11:59:25: 10000000 INFO @ Fri, 10 Dec 2021 11:59:25: 6000000 INFO @ Fri, 10 Dec 2021 11:59:28: 18000000 INFO @ Fri, 10 Dec 2021 11:59:31: 11000000 INFO @ Fri, 10 Dec 2021 11:59:31: 7000000 INFO @ Fri, 10 Dec 2021 11:59:34: #1 tag size is determined as 43 bps INFO @ Fri, 10 Dec 2021 11:59:34: #1 tag size = 43 INFO @ Fri, 10 Dec 2021 11:59:34: #1 total tags in treatment: 8468790 INFO @ Fri, 10 Dec 2021 11:59:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:59:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:59:34: #1 tags after filtering in treatment: 8212525 INFO @ Fri, 10 Dec 2021 11:59:34: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 10 Dec 2021 11:59:34: #1 finished! INFO @ Fri, 10 Dec 2021 11:59:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:59:35: #2 number of paired peaks: 97 WARNING @ Fri, 10 Dec 2021 11:59:35: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:59:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:59:37: 8000000 INFO @ Fri, 10 Dec 2021 11:59:38: 12000000 INFO @ Fri, 10 Dec 2021 11:59:43: 9000000 INFO @ Fri, 10 Dec 2021 11:59:44: 13000000 INFO @ Fri, 10 Dec 2021 11:59:48: 10000000 INFO @ Fri, 10 Dec 2021 11:59:50: 14000000 INFO @ Fri, 10 Dec 2021 11:59:54: 11000000 INFO @ Fri, 10 Dec 2021 11:59:56: 15000000 INFO @ Fri, 10 Dec 2021 11:59:59: 12000000 INFO @ Fri, 10 Dec 2021 12:00:02: 16000000 INFO @ Fri, 10 Dec 2021 12:00:05: 13000000 INFO @ Fri, 10 Dec 2021 12:00:08: 17000000 INFO @ Fri, 10 Dec 2021 12:00:10: 14000000 INFO @ Fri, 10 Dec 2021 12:00:14: 18000000 INFO @ Fri, 10 Dec 2021 12:00:16: 15000000 INFO @ Fri, 10 Dec 2021 12:00:20: #1 tag size is determined as 43 bps INFO @ Fri, 10 Dec 2021 12:00:20: #1 tag size = 43 INFO @ Fri, 10 Dec 2021 12:00:20: #1 total tags in treatment: 8468790 INFO @ Fri, 10 Dec 2021 12:00:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:00:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:00:20: #1 tags after filtering in treatment: 8212525 INFO @ Fri, 10 Dec 2021 12:00:20: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 10 Dec 2021 12:00:20: #1 finished! INFO @ Fri, 10 Dec 2021 12:00:20: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:00:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:00:21: #2 number of paired peaks: 97 WARNING @ Fri, 10 Dec 2021 12:00:21: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:00:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:00:21: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:00:26: 17000000 INFO @ Fri, 10 Dec 2021 12:00:31: 18000000 INFO @ Fri, 10 Dec 2021 12:00:36: #1 tag size is determined as 43 bps INFO @ Fri, 10 Dec 2021 12:00:36: #1 tag size = 43 INFO @ Fri, 10 Dec 2021 12:00:36: #1 total tags in treatment: 8468790 INFO @ Fri, 10 Dec 2021 12:00:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:00:36: #1 tags after filtering in treatment: 8212525 INFO @ Fri, 10 Dec 2021 12:00:36: #1 Redundant rate of treatment: 0.03 INFO @ Fri, 10 Dec 2021 12:00:36: #1 finished! INFO @ Fri, 10 Dec 2021 12:00:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:00:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:00:37: #2 number of paired peaks: 97 WARNING @ Fri, 10 Dec 2021 12:00:37: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 12:00:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8076974/SRX8076974.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。