Job ID = 6626998 SRX = SRX7988221 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 24530815 spots for SRR11409519/SRR11409519.sra Written 24530815 spots for SRR11409519/SRR11409519.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627382 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:06:21 24530815 reads; of these: 24530815 (100.00%) were paired; of these: 2925040 (11.92%) aligned concordantly 0 times 14482946 (59.04%) aligned concordantly exactly 1 time 7122829 (29.04%) aligned concordantly >1 times ---- 2925040 pairs aligned concordantly 0 times; of these: 1091721 (37.32%) aligned discordantly 1 time ---- 1833319 pairs aligned 0 times concordantly or discordantly; of these: 3666638 mates make up the pairs; of these: 1990883 (54.30%) aligned 0 times 646131 (17.62%) aligned exactly 1 time 1029624 (28.08%) aligned >1 times 95.94% overall alignment rate Time searching: 01:06:21 Overall time: 01:06:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1723004 / 22589016 = 0.0763 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:34:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:34:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:34:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:34:54: 1000000 INFO @ Tue, 14 Jul 2020 10:34:59: 2000000 INFO @ Tue, 14 Jul 2020 10:35:05: 3000000 INFO @ Tue, 14 Jul 2020 10:35:10: 4000000 INFO @ Tue, 14 Jul 2020 10:35:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:35:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:35:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:35:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:35:21: 6000000 INFO @ Tue, 14 Jul 2020 10:35:26: 1000000 INFO @ Tue, 14 Jul 2020 10:35:27: 7000000 INFO @ Tue, 14 Jul 2020 10:35:32: 2000000 INFO @ Tue, 14 Jul 2020 10:35:33: 8000000 INFO @ Tue, 14 Jul 2020 10:35:39: 9000000 INFO @ Tue, 14 Jul 2020 10:35:39: 3000000 INFO @ Tue, 14 Jul 2020 10:35:45: 10000000 INFO @ Tue, 14 Jul 2020 10:35:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:35:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:35:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:35:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:35:51: 11000000 INFO @ Tue, 14 Jul 2020 10:35:53: 5000000 INFO @ Tue, 14 Jul 2020 10:35:55: 1000000 INFO @ Tue, 14 Jul 2020 10:35:57: 12000000 INFO @ Tue, 14 Jul 2020 10:35:59: 6000000 INFO @ Tue, 14 Jul 2020 10:36:01: 2000000 INFO @ Tue, 14 Jul 2020 10:36:03: 13000000 INFO @ Tue, 14 Jul 2020 10:36:06: 7000000 INFO @ Tue, 14 Jul 2020 10:36:07: 3000000 INFO @ Tue, 14 Jul 2020 10:36:09: 14000000 INFO @ Tue, 14 Jul 2020 10:36:13: 4000000 INFO @ Tue, 14 Jul 2020 10:36:14: 15000000 INFO @ Tue, 14 Jul 2020 10:36:15: 8000000 INFO @ Tue, 14 Jul 2020 10:36:19: 5000000 INFO @ Tue, 14 Jul 2020 10:36:20: 16000000 INFO @ Tue, 14 Jul 2020 10:36:25: 9000000 INFO @ Tue, 14 Jul 2020 10:36:25: 6000000 INFO @ Tue, 14 Jul 2020 10:36:26: 17000000 INFO @ Tue, 14 Jul 2020 10:36:31: 7000000 INFO @ Tue, 14 Jul 2020 10:36:32: 18000000 INFO @ Tue, 14 Jul 2020 10:36:34: 10000000 INFO @ Tue, 14 Jul 2020 10:36:37: 8000000 INFO @ Tue, 14 Jul 2020 10:36:38: 19000000 INFO @ Tue, 14 Jul 2020 10:36:43: 9000000 INFO @ Tue, 14 Jul 2020 10:36:43: 11000000 INFO @ Tue, 14 Jul 2020 10:36:44: 20000000 INFO @ Tue, 14 Jul 2020 10:36:49: 10000000 INFO @ Tue, 14 Jul 2020 10:36:50: 21000000 INFO @ Tue, 14 Jul 2020 10:36:52: 12000000 INFO @ Tue, 14 Jul 2020 10:36:55: 11000000 INFO @ Tue, 14 Jul 2020 10:36:56: 22000000 INFO @ Tue, 14 Jul 2020 10:37:01: 13000000 INFO @ Tue, 14 Jul 2020 10:37:01: 12000000 INFO @ Tue, 14 Jul 2020 10:37:02: 23000000 INFO @ Tue, 14 Jul 2020 10:37:08: 13000000 INFO @ Tue, 14 Jul 2020 10:37:08: 24000000 INFO @ Tue, 14 Jul 2020 10:37:10: 14000000 INFO @ Tue, 14 Jul 2020 10:37:14: 25000000 INFO @ Tue, 14 Jul 2020 10:37:14: 14000000 INFO @ Tue, 14 Jul 2020 10:37:17: 15000000 INFO @ Tue, 14 Jul 2020 10:37:19: 26000000 INFO @ Tue, 14 Jul 2020 10:37:20: 15000000 INFO @ Tue, 14 Jul 2020 10:37:24: 16000000 INFO @ Tue, 14 Jul 2020 10:37:25: 27000000 INFO @ Tue, 14 Jul 2020 10:37:26: 16000000 INFO @ Tue, 14 Jul 2020 10:37:31: 17000000 INFO @ Tue, 14 Jul 2020 10:37:31: 28000000 INFO @ Tue, 14 Jul 2020 10:37:33: 17000000 INFO @ Tue, 14 Jul 2020 10:37:37: 29000000 INFO @ Tue, 14 Jul 2020 10:37:37: 18000000 INFO @ Tue, 14 Jul 2020 10:37:39: 18000000 INFO @ Tue, 14 Jul 2020 10:37:43: 30000000 INFO @ Tue, 14 Jul 2020 10:37:44: 19000000 INFO @ Tue, 14 Jul 2020 10:37:45: 19000000 INFO @ Tue, 14 Jul 2020 10:37:49: 31000000 INFO @ Tue, 14 Jul 2020 10:37:51: 20000000 INFO @ Tue, 14 Jul 2020 10:37:52: 20000000 INFO @ Tue, 14 Jul 2020 10:37:55: 32000000 INFO @ Tue, 14 Jul 2020 10:37:57: 21000000 INFO @ Tue, 14 Jul 2020 10:37:58: 21000000 INFO @ Tue, 14 Jul 2020 10:38:01: 33000000 INFO @ Tue, 14 Jul 2020 10:38:04: 22000000 INFO @ Tue, 14 Jul 2020 10:38:05: 22000000 INFO @ Tue, 14 Jul 2020 10:38:07: 34000000 INFO @ Tue, 14 Jul 2020 10:38:11: 23000000 INFO @ Tue, 14 Jul 2020 10:38:12: 23000000 INFO @ Tue, 14 Jul 2020 10:38:13: 35000000 INFO @ Tue, 14 Jul 2020 10:38:18: 24000000 INFO @ Tue, 14 Jul 2020 10:38:18: 24000000 INFO @ Tue, 14 Jul 2020 10:38:19: 36000000 INFO @ Tue, 14 Jul 2020 10:38:24: 25000000 INFO @ Tue, 14 Jul 2020 10:38:25: 37000000 INFO @ Tue, 14 Jul 2020 10:38:25: 25000000 INFO @ Tue, 14 Jul 2020 10:38:31: 26000000 INFO @ Tue, 14 Jul 2020 10:38:31: 38000000 INFO @ Tue, 14 Jul 2020 10:38:32: 26000000 INFO @ Tue, 14 Jul 2020 10:38:37: 39000000 INFO @ Tue, 14 Jul 2020 10:38:38: 27000000 INFO @ Tue, 14 Jul 2020 10:38:38: 27000000 INFO @ Tue, 14 Jul 2020 10:38:43: 40000000 INFO @ Tue, 14 Jul 2020 10:38:44: 28000000 INFO @ Tue, 14 Jul 2020 10:38:45: 28000000 INFO @ Tue, 14 Jul 2020 10:38:49: 41000000 INFO @ Tue, 14 Jul 2020 10:38:51: 29000000 INFO @ Tue, 14 Jul 2020 10:38:52: 29000000 INFO @ Tue, 14 Jul 2020 10:38:55: 42000000 INFO @ Tue, 14 Jul 2020 10:38:58: 30000000 INFO @ Tue, 14 Jul 2020 10:38:59: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:39:01: 43000000 INFO @ Tue, 14 Jul 2020 10:39:05: 31000000 INFO @ Tue, 14 Jul 2020 10:39:05: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 10:39:05: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 10:39:05: #1 total tags in treatment: 19950143 INFO @ Tue, 14 Jul 2020 10:39:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:39:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:39:05: #1 tags after filtering in treatment: 16989658 INFO @ Tue, 14 Jul 2020 10:39:05: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 14 Jul 2020 10:39:05: #1 finished! INFO @ Tue, 14 Jul 2020 10:39:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:39:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:39:05: 31000000 INFO @ Tue, 14 Jul 2020 10:39:06: #2 number of paired peaks: 21 WARNING @ Tue, 14 Jul 2020 10:39:06: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:39:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:39:11: 32000000 INFO @ Tue, 14 Jul 2020 10:39:12: 32000000 INFO @ Tue, 14 Jul 2020 10:39:18: 33000000 INFO @ Tue, 14 Jul 2020 10:39:19: 33000000 INFO @ Tue, 14 Jul 2020 10:39:25: 34000000 INFO @ Tue, 14 Jul 2020 10:39:26: 34000000 INFO @ Tue, 14 Jul 2020 10:39:31: 35000000 INFO @ Tue, 14 Jul 2020 10:39:32: 35000000 INFO @ Tue, 14 Jul 2020 10:39:38: 36000000 INFO @ Tue, 14 Jul 2020 10:39:39: 36000000 INFO @ Tue, 14 Jul 2020 10:39:45: 37000000 INFO @ Tue, 14 Jul 2020 10:39:46: 37000000 INFO @ Tue, 14 Jul 2020 10:39:52: 38000000 INFO @ Tue, 14 Jul 2020 10:39:52: 38000000 INFO @ Tue, 14 Jul 2020 10:39:58: 39000000 INFO @ Tue, 14 Jul 2020 10:39:59: 39000000 INFO @ Tue, 14 Jul 2020 10:40:05: 40000000 INFO @ Tue, 14 Jul 2020 10:40:06: 40000000 INFO @ Tue, 14 Jul 2020 10:40:12: 41000000 INFO @ Tue, 14 Jul 2020 10:40:13: 41000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:40:18: 42000000 INFO @ Tue, 14 Jul 2020 10:40:19: 42000000 INFO @ Tue, 14 Jul 2020 10:40:25: 43000000 INFO @ Tue, 14 Jul 2020 10:40:26: 43000000 INFO @ Tue, 14 Jul 2020 10:40:29: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 10:40:29: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 10:40:29: #1 total tags in treatment: 19950143 INFO @ Tue, 14 Jul 2020 10:40:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:40:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:40:30: #1 tags after filtering in treatment: 16989658 INFO @ Tue, 14 Jul 2020 10:40:30: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 14 Jul 2020 10:40:30: #1 finished! INFO @ Tue, 14 Jul 2020 10:40:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:40:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:40:30: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 10:40:30: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 10:40:30: #1 total tags in treatment: 19950143 INFO @ Tue, 14 Jul 2020 10:40:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:40:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:40:31: #1 tags after filtering in treatment: 16989658 INFO @ Tue, 14 Jul 2020 10:40:31: #1 Redundant rate of treatment: 0.15 INFO @ Tue, 14 Jul 2020 10:40:31: #1 finished! INFO @ Tue, 14 Jul 2020 10:40:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:40:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:40:31: #2 number of paired peaks: 21 WARNING @ Tue, 14 Jul 2020 10:40:31: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:40:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:40:32: #2 number of paired peaks: 21 WARNING @ Tue, 14 Jul 2020 10:40:32: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:40:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988221/SRX7988221.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling