Job ID = 6626984 SRX = SRX7975005 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:09:28 prefetch.2.10.7: 1) Downloading 'SRR11396274'... 2020-07-14T00:09:28 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:12:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:12:39 prefetch.2.10.7: 1) 'SRR11396274' was downloaded successfully Read 26719022 spots for SRR11396274/SRR11396274.sra Written 26719022 spots for SRR11396274/SRR11396274.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627138 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:08 26719022 reads; of these: 26719022 (100.00%) were unpaired; of these: 9023938 (33.77%) aligned 0 times 13475731 (50.43%) aligned exactly 1 time 4219353 (15.79%) aligned >1 times 66.23% overall alignment rate Time searching: 00:07:08 Overall time: 00:07:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3320663 / 17695084 = 0.1877 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:26:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:26:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:26:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:26:35: 1000000 INFO @ Tue, 14 Jul 2020 09:26:41: 2000000 INFO @ Tue, 14 Jul 2020 09:26:46: 3000000 INFO @ Tue, 14 Jul 2020 09:26:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:26:57: 5000000 INFO @ Tue, 14 Jul 2020 09:26:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:26:59: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:26:59: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:27:03: 6000000 INFO @ Tue, 14 Jul 2020 09:27:05: 1000000 INFO @ Tue, 14 Jul 2020 09:27:09: 7000000 INFO @ Tue, 14 Jul 2020 09:27:11: 2000000 INFO @ Tue, 14 Jul 2020 09:27:15: 8000000 INFO @ Tue, 14 Jul 2020 09:27:17: 3000000 INFO @ Tue, 14 Jul 2020 09:27:21: 9000000 INFO @ Tue, 14 Jul 2020 09:27:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:27:27: 10000000 INFO @ Tue, 14 Jul 2020 09:27:28: 5000000 INFO @ Tue, 14 Jul 2020 09:27:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:27:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:27:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:27:33: 11000000 INFO @ Tue, 14 Jul 2020 09:27:34: 6000000 INFO @ Tue, 14 Jul 2020 09:27:35: 1000000 INFO @ Tue, 14 Jul 2020 09:27:39: 12000000 INFO @ Tue, 14 Jul 2020 09:27:40: 7000000 INFO @ Tue, 14 Jul 2020 09:27:41: 2000000 INFO @ Tue, 14 Jul 2020 09:27:45: 13000000 INFO @ Tue, 14 Jul 2020 09:27:46: 8000000 INFO @ Tue, 14 Jul 2020 09:27:47: 3000000 INFO @ Tue, 14 Jul 2020 09:27:51: 14000000 INFO @ Tue, 14 Jul 2020 09:27:52: 9000000 INFO @ Tue, 14 Jul 2020 09:27:53: 4000000 INFO @ Tue, 14 Jul 2020 09:27:53: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:27:53: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:27:53: #1 total tags in treatment: 14374421 INFO @ Tue, 14 Jul 2020 09:27:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:27:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:27:54: #1 tags after filtering in treatment: 14374421 INFO @ Tue, 14 Jul 2020 09:27:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:27:54: #1 finished! INFO @ Tue, 14 Jul 2020 09:27:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:27:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:27:55: #2 number of paired peaks: 38 WARNING @ Tue, 14 Jul 2020 09:27:55: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:27:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:27:58: 10000000 INFO @ Tue, 14 Jul 2020 09:27:59: 5000000 INFO @ Tue, 14 Jul 2020 09:28:05: 11000000 INFO @ Tue, 14 Jul 2020 09:28:05: 6000000 INFO @ Tue, 14 Jul 2020 09:28:10: 12000000 INFO @ Tue, 14 Jul 2020 09:28:11: 7000000 INFO @ Tue, 14 Jul 2020 09:28:16: 13000000 INFO @ Tue, 14 Jul 2020 09:28:17: 8000000 INFO @ Tue, 14 Jul 2020 09:28:22: 14000000 INFO @ Tue, 14 Jul 2020 09:28:23: 9000000 INFO @ Tue, 14 Jul 2020 09:28:25: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:28:25: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:28:25: #1 total tags in treatment: 14374421 INFO @ Tue, 14 Jul 2020 09:28:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:28:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:28:25: #1 tags after filtering in treatment: 14374421 INFO @ Tue, 14 Jul 2020 09:28:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:28:25: #1 finished! INFO @ Tue, 14 Jul 2020 09:28:25: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:28:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:28:26: #2 number of paired peaks: 38 WARNING @ Tue, 14 Jul 2020 09:28:26: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:28:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:28:28: 10000000 INFO @ Tue, 14 Jul 2020 09:28:34: 11000000 INFO @ Tue, 14 Jul 2020 09:28:40: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:28:46: 13000000 INFO @ Tue, 14 Jul 2020 09:28:51: 14000000 INFO @ Tue, 14 Jul 2020 09:28:54: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:28:54: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:28:54: #1 total tags in treatment: 14374421 INFO @ Tue, 14 Jul 2020 09:28:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:28:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:28:54: #1 tags after filtering in treatment: 14374421 INFO @ Tue, 14 Jul 2020 09:28:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:28:54: #1 finished! INFO @ Tue, 14 Jul 2020 09:28:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:28:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:28:55: #2 number of paired peaks: 38 WARNING @ Tue, 14 Jul 2020 09:28:55: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:28:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975005/SRX7975005.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。