Job ID = 8071292 SRX = SRX7917544 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T04:26:33 prefetch.2.10.7: 1) Downloading 'SRR11313125'... 2020-08-08T04:26:33 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T04:27:47 prefetch.2.10.7: HTTPS download succeed 2020-08-08T04:27:48 prefetch.2.10.7: 'SRR11313125' is valid 2020-08-08T04:27:48 prefetch.2.10.7: 1) 'SRR11313125' was downloaded successfully Read 2551506 spots for SRR11313125/SRR11313125.sra Written 2551506 spots for SRR11313125/SRR11313125.sra fastq に変換しました。 bowtie でマッピング中... Your job 8072457 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:01 2551506 reads; of these: 2551506 (100.00%) were paired; of these: 457933 (17.95%) aligned concordantly 0 times 1759827 (68.97%) aligned concordantly exactly 1 time 333746 (13.08%) aligned concordantly >1 times ---- 457933 pairs aligned concordantly 0 times; of these: 208766 (45.59%) aligned discordantly 1 time ---- 249167 pairs aligned 0 times concordantly or discordantly; of these: 498334 mates make up the pairs; of these: 213026 (42.75%) aligned 0 times 143597 (28.82%) aligned exactly 1 time 141711 (28.44%) aligned >1 times 95.83% overall alignment rate Time searching: 00:04:01 Overall time: 00:04:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1069199 / 2295756 = 0.4657 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:33:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:33:40: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:33:40: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:33:46: 1000000 INFO @ Sat, 08 Aug 2020 13:33:51: 2000000 INFO @ Sat, 08 Aug 2020 13:33:55: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:33:55: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:33:55: #1 total tags in treatment: 1066012 INFO @ Sat, 08 Aug 2020 13:33:55: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:33:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:33:55: #1 tags after filtering in treatment: 756028 INFO @ Sat, 08 Aug 2020 13:33:55: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 08 Aug 2020 13:33:55: #1 finished! INFO @ Sat, 08 Aug 2020 13:33:55: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:33:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:33:55: #2 number of paired peaks: 2533 INFO @ Sat, 08 Aug 2020 13:33:55: start model_add_line... INFO @ Sat, 08 Aug 2020 13:33:55: start X-correlation... INFO @ Sat, 08 Aug 2020 13:33:55: end of X-cor INFO @ Sat, 08 Aug 2020 13:33:55: #2 finished! INFO @ Sat, 08 Aug 2020 13:33:55: #2 predicted fragment length is 184 bps INFO @ Sat, 08 Aug 2020 13:33:55: #2 alternative fragment length(s) may be 1,60,151,184 bps INFO @ Sat, 08 Aug 2020 13:33:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.05_model.r INFO @ Sat, 08 Aug 2020 13:33:55: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:33:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:33:57: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:33:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.05_peaks.xls INFO @ Sat, 08 Aug 2020 13:33:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.05_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:33:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.05_summits.bed INFO @ Sat, 08 Aug 2020 13:33:58: Done! pass1 - making usageList (2 chroms): 0 millis pass2 - checking and writing primary data (10 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:34:10: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:34:10: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:34:15: 1000000 INFO @ Sat, 08 Aug 2020 13:34:20: 2000000 INFO @ Sat, 08 Aug 2020 13:34:24: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:34:24: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:34:24: #1 total tags in treatment: 1066012 INFO @ Sat, 08 Aug 2020 13:34:24: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:34:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:34:24: #1 tags after filtering in treatment: 756028 INFO @ Sat, 08 Aug 2020 13:34:24: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 08 Aug 2020 13:34:24: #1 finished! INFO @ Sat, 08 Aug 2020 13:34:24: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:34:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:34:25: #2 number of paired peaks: 2533 INFO @ Sat, 08 Aug 2020 13:34:25: start model_add_line... INFO @ Sat, 08 Aug 2020 13:34:25: start X-correlation... INFO @ Sat, 08 Aug 2020 13:34:25: end of X-cor INFO @ Sat, 08 Aug 2020 13:34:25: #2 finished! INFO @ Sat, 08 Aug 2020 13:34:25: #2 predicted fragment length is 184 bps INFO @ Sat, 08 Aug 2020 13:34:25: #2 alternative fragment length(s) may be 1,60,151,184 bps INFO @ Sat, 08 Aug 2020 13:34:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.10_model.r INFO @ Sat, 08 Aug 2020 13:34:25: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:34:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:34:26: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:34:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.10_peaks.xls INFO @ Sat, 08 Aug 2020 13:34:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.10_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:34:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.10_summits.bed INFO @ Sat, 08 Aug 2020 13:34:27: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:34:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:34:40: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:34:40: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:34:45: 1000000 INFO @ Sat, 08 Aug 2020 13:34:50: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 13:34:54: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:34:54: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:34:54: #1 total tags in treatment: 1066012 INFO @ Sat, 08 Aug 2020 13:34:54: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:34:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:34:54: #1 tags after filtering in treatment: 756028 INFO @ Sat, 08 Aug 2020 13:34:54: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 08 Aug 2020 13:34:54: #1 finished! INFO @ Sat, 08 Aug 2020 13:34:54: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:34:54: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 13:34:54: #2 number of paired peaks: 2533 INFO @ Sat, 08 Aug 2020 13:34:54: start model_add_line... INFO @ Sat, 08 Aug 2020 13:34:54: start X-correlation... INFO @ Sat, 08 Aug 2020 13:34:54: end of X-cor INFO @ Sat, 08 Aug 2020 13:34:54: #2 finished! INFO @ Sat, 08 Aug 2020 13:34:54: #2 predicted fragment length is 184 bps INFO @ Sat, 08 Aug 2020 13:34:54: #2 alternative fragment length(s) may be 1,60,151,184 bps INFO @ Sat, 08 Aug 2020 13:34:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.20_model.r INFO @ Sat, 08 Aug 2020 13:34:54: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:34:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:34:56: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:34:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.20_peaks.xls INFO @ Sat, 08 Aug 2020 13:34:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.20_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:34:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917544/SRX7917544.20_summits.bed INFO @ Sat, 08 Aug 2020 13:34:56: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling