Job ID = 8070965 SRX = SRX7917492 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T04:16:04 prefetch.2.10.7: 1) Downloading 'SRR11313073'... 2020-08-08T04:16:04 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T04:16:26 prefetch.2.10.7: HTTPS download succeed 2020-08-08T04:16:26 prefetch.2.10.7: 'SRR11313073' is valid 2020-08-08T04:16:26 prefetch.2.10.7: 1) 'SRR11313073' was downloaded successfully Read 1392479 spots for SRR11313073/SRR11313073.sra Written 1392479 spots for SRR11313073/SRR11313073.sra fastq に変換しました。 bowtie でマッピング中... Your job 8071795 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:15 1392479 reads; of these: 1392479 (100.00%) were paired; of these: 285947 (20.54%) aligned concordantly 0 times 887772 (63.75%) aligned concordantly exactly 1 time 218760 (15.71%) aligned concordantly >1 times ---- 285947 pairs aligned concordantly 0 times; of these: 94476 (33.04%) aligned discordantly 1 time ---- 191471 pairs aligned 0 times concordantly or discordantly; of these: 382942 mates make up the pairs; of these: 244235 (63.78%) aligned 0 times 68304 (17.84%) aligned exactly 1 time 70403 (18.38%) aligned >1 times 91.23% overall alignment rate Time searching: 00:02:15 Overall time: 00:02:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 470638 / 1196389 = 0.3934 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:19:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:19:50: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:19:50: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:19:56: 1000000 INFO @ Sat, 08 Aug 2020 13:20:00: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:20:00: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:20:00: #1 total tags in treatment: 658554 INFO @ Sat, 08 Aug 2020 13:20:00: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:20:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:20:00: #1 tags after filtering in treatment: 551713 INFO @ Sat, 08 Aug 2020 13:20:00: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 08 Aug 2020 13:20:00: #1 finished! INFO @ Sat, 08 Aug 2020 13:20:00: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:20:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:20:00: #2 number of paired peaks: 1312 INFO @ Sat, 08 Aug 2020 13:20:00: start model_add_line... INFO @ Sat, 08 Aug 2020 13:20:00: start X-correlation... INFO @ Sat, 08 Aug 2020 13:20:00: end of X-cor INFO @ Sat, 08 Aug 2020 13:20:00: #2 finished! INFO @ Sat, 08 Aug 2020 13:20:00: #2 predicted fragment length is 161 bps INFO @ Sat, 08 Aug 2020 13:20:00: #2 alternative fragment length(s) may be 2,37,66,99,161,185,217,249 bps INFO @ Sat, 08 Aug 2020 13:20:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.05_model.r INFO @ Sat, 08 Aug 2020 13:20:00: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:20:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:20:01: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:20:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.05_peaks.xls INFO @ Sat, 08 Aug 2020 13:20:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.05_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:20:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.05_summits.bed INFO @ Sat, 08 Aug 2020 13:20:02: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:20:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:20:20: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:20:20: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:20:26: 1000000 INFO @ Sat, 08 Aug 2020 13:20:29: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:20:29: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:20:29: #1 total tags in treatment: 658554 INFO @ Sat, 08 Aug 2020 13:20:29: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:20:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:20:29: #1 tags after filtering in treatment: 551713 INFO @ Sat, 08 Aug 2020 13:20:29: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 08 Aug 2020 13:20:29: #1 finished! INFO @ Sat, 08 Aug 2020 13:20:29: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:20:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:20:29: #2 number of paired peaks: 1312 INFO @ Sat, 08 Aug 2020 13:20:29: start model_add_line... INFO @ Sat, 08 Aug 2020 13:20:29: start X-correlation... INFO @ Sat, 08 Aug 2020 13:20:29: end of X-cor INFO @ Sat, 08 Aug 2020 13:20:29: #2 finished! INFO @ Sat, 08 Aug 2020 13:20:29: #2 predicted fragment length is 161 bps INFO @ Sat, 08 Aug 2020 13:20:29: #2 alternative fragment length(s) may be 2,37,66,99,161,185,217,249 bps INFO @ Sat, 08 Aug 2020 13:20:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.10_model.r INFO @ Sat, 08 Aug 2020 13:20:29: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:20:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:20:30: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:20:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.10_peaks.xls INFO @ Sat, 08 Aug 2020 13:20:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.10_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:20:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.10_summits.bed INFO @ Sat, 08 Aug 2020 13:20:30: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:20:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:20:50: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:20:50: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 13:20:57: 1000000 BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 13:21:01: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:21:01: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:21:01: #1 total tags in treatment: 658554 INFO @ Sat, 08 Aug 2020 13:21:01: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:21:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:21:01: #1 tags after filtering in treatment: 551713 INFO @ Sat, 08 Aug 2020 13:21:01: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 08 Aug 2020 13:21:01: #1 finished! INFO @ Sat, 08 Aug 2020 13:21:01: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:21:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:21:01: #2 number of paired peaks: 1312 INFO @ Sat, 08 Aug 2020 13:21:01: start model_add_line... INFO @ Sat, 08 Aug 2020 13:21:01: start X-correlation... INFO @ Sat, 08 Aug 2020 13:21:01: end of X-cor INFO @ Sat, 08 Aug 2020 13:21:01: #2 finished! INFO @ Sat, 08 Aug 2020 13:21:01: #2 predicted fragment length is 161 bps INFO @ Sat, 08 Aug 2020 13:21:01: #2 alternative fragment length(s) may be 2,37,66,99,161,185,217,249 bps INFO @ Sat, 08 Aug 2020 13:21:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.20_model.r INFO @ Sat, 08 Aug 2020 13:21:01: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:21:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:21:02: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:21:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.20_peaks.xls INFO @ Sat, 08 Aug 2020 13:21:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.20_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:21:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917492/SRX7917492.20_summits.bed INFO @ Sat, 08 Aug 2020 13:21:02: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling