Job ID = 8069853 SRX = SRX7917469 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T03:56:58 prefetch.2.10.7: 1) Downloading 'SRR11313050'... 2020-08-08T03:56:58 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:57:28 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:57:28 prefetch.2.10.7: 'SRR11313050' is valid 2020-08-08T03:57:28 prefetch.2.10.7: 1) 'SRR11313050' was downloaded successfully Read 3338068 spots for SRR11313050/SRR11313050.sra Written 3338068 spots for SRR11313050/SRR11313050.sra fastq に変換しました。 bowtie でマッピング中... Your job 8071070 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:07 3338068 reads; of these: 3338068 (100.00%) were paired; of these: 839559 (25.15%) aligned concordantly 0 times 1866736 (55.92%) aligned concordantly exactly 1 time 631773 (18.93%) aligned concordantly >1 times ---- 839559 pairs aligned concordantly 0 times; of these: 321359 (38.28%) aligned discordantly 1 time ---- 518200 pairs aligned 0 times concordantly or discordantly; of these: 1036400 mates make up the pairs; of these: 570682 (55.06%) aligned 0 times 185677 (17.92%) aligned exactly 1 time 280041 (27.02%) aligned >1 times 91.45% overall alignment rate Time searching: 00:06:07 Overall time: 00:06:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 949663 / 2808853 = 0.3381 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:05:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:05:38: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:05:38: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:05:43: 1000000 INFO @ Sat, 08 Aug 2020 13:05:48: 2000000 INFO @ Sat, 08 Aug 2020 13:05:53: 3000000 INFO @ Sat, 08 Aug 2020 13:05:59: 4000000 INFO @ Sat, 08 Aug 2020 13:06:00: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:06:00: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:06:00: #1 total tags in treatment: 1601861 INFO @ Sat, 08 Aug 2020 13:06:00: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:06:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:06:00: #1 tags after filtering in treatment: 1263540 INFO @ Sat, 08 Aug 2020 13:06:00: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 08 Aug 2020 13:06:00: #1 finished! INFO @ Sat, 08 Aug 2020 13:06:00: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:06:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:06:00: #2 number of paired peaks: 4 WARNING @ Sat, 08 Aug 2020 13:06:00: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 13:06:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:06:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:06:08: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:06:08: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:06:14: 1000000 INFO @ Sat, 08 Aug 2020 13:06:21: 2000000 INFO @ Sat, 08 Aug 2020 13:06:27: 3000000 INFO @ Sat, 08 Aug 2020 13:06:34: 4000000 INFO @ Sat, 08 Aug 2020 13:06:35: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:06:35: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:06:35: #1 total tags in treatment: 1601861 INFO @ Sat, 08 Aug 2020 13:06:35: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:06:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:06:35: #1 tags after filtering in treatment: 1263540 INFO @ Sat, 08 Aug 2020 13:06:35: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 08 Aug 2020 13:06:35: #1 finished! INFO @ Sat, 08 Aug 2020 13:06:35: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:06:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:06:35: #2 number of paired peaks: 4 WARNING @ Sat, 08 Aug 2020 13:06:35: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 13:06:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:06:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:06:38: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:06:38: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:06:43: 1000000 INFO @ Sat, 08 Aug 2020 13:06:48: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 13:06:53: 3000000 BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 13:06:59: 4000000 INFO @ Sat, 08 Aug 2020 13:07:00: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:07:00: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:07:00: #1 total tags in treatment: 1601861 INFO @ Sat, 08 Aug 2020 13:07:00: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:07:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:07:00: #1 tags after filtering in treatment: 1263540 INFO @ Sat, 08 Aug 2020 13:07:00: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 08 Aug 2020 13:07:00: #1 finished! INFO @ Sat, 08 Aug 2020 13:07:00: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:07:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:07:00: #2 number of paired peaks: 4 WARNING @ Sat, 08 Aug 2020 13:07:00: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 13:07:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917469/SRX7917469.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling