Job ID = 8069843 SRX = SRX7917466 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T03:55:45 prefetch.2.10.7: 1) Downloading 'SRR11313047'... 2020-08-08T03:55:45 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:56:29 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:56:29 prefetch.2.10.7: 'SRR11313047' is valid 2020-08-08T03:56:29 prefetch.2.10.7: 1) 'SRR11313047' was downloaded successfully Read 2252515 spots for SRR11313047/SRR11313047.sra Written 2252515 spots for SRR11313047/SRR11313047.sra fastq に変換しました。 bowtie でマッピング中... Your job 8070837 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:36 2252515 reads; of these: 2252515 (100.00%) were paired; of these: 464102 (20.60%) aligned concordantly 0 times 1569602 (69.68%) aligned concordantly exactly 1 time 218811 (9.71%) aligned concordantly >1 times ---- 464102 pairs aligned concordantly 0 times; of these: 212768 (45.85%) aligned discordantly 1 time ---- 251334 pairs aligned 0 times concordantly or discordantly; of these: 502668 mates make up the pairs; of these: 320813 (63.82%) aligned 0 times 96701 (19.24%) aligned exactly 1 time 85154 (16.94%) aligned >1 times 92.88% overall alignment rate Time searching: 00:02:36 Overall time: 00:02:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 951371 / 1993430 = 0.4773 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:00:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:00:34: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:00:34: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:00:40: 1000000 INFO @ Sat, 08 Aug 2020 13:00:47: 2000000 INFO @ Sat, 08 Aug 2020 13:00:49: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:00:49: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:00:49: #1 total tags in treatment: 876137 INFO @ Sat, 08 Aug 2020 13:00:49: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:00:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:00:49: #1 tags after filtering in treatment: 613487 INFO @ Sat, 08 Aug 2020 13:00:49: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 08 Aug 2020 13:00:49: #1 finished! INFO @ Sat, 08 Aug 2020 13:00:49: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:00:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:00:49: #2 number of paired peaks: 4970 INFO @ Sat, 08 Aug 2020 13:00:49: start model_add_line... INFO @ Sat, 08 Aug 2020 13:00:49: start X-correlation... INFO @ Sat, 08 Aug 2020 13:00:49: end of X-cor INFO @ Sat, 08 Aug 2020 13:00:49: #2 finished! INFO @ Sat, 08 Aug 2020 13:00:49: #2 predicted fragment length is 171 bps INFO @ Sat, 08 Aug 2020 13:00:49: #2 alternative fragment length(s) may be 3,171,198 bps INFO @ Sat, 08 Aug 2020 13:00:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.05_model.r INFO @ Sat, 08 Aug 2020 13:00:49: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:00:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:00:50: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:00:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.05_peaks.xls INFO @ Sat, 08 Aug 2020 13:00:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.05_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:00:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.05_summits.bed INFO @ Sat, 08 Aug 2020 13:00:51: Done! pass1 - making usageList (2 chroms): 0 millis pass2 - checking and writing primary data (14 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:01:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:01:04: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:01:04: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:01:10: 1000000 INFO @ Sat, 08 Aug 2020 13:01:17: 2000000 INFO @ Sat, 08 Aug 2020 13:01:18: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:01:18: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:01:18: #1 total tags in treatment: 876137 INFO @ Sat, 08 Aug 2020 13:01:18: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:01:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:01:18: #1 tags after filtering in treatment: 613487 INFO @ Sat, 08 Aug 2020 13:01:18: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 08 Aug 2020 13:01:18: #1 finished! INFO @ Sat, 08 Aug 2020 13:01:18: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:01:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:01:19: #2 number of paired peaks: 4970 INFO @ Sat, 08 Aug 2020 13:01:19: start model_add_line... INFO @ Sat, 08 Aug 2020 13:01:19: start X-correlation... INFO @ Sat, 08 Aug 2020 13:01:19: end of X-cor INFO @ Sat, 08 Aug 2020 13:01:19: #2 finished! INFO @ Sat, 08 Aug 2020 13:01:19: #2 predicted fragment length is 171 bps INFO @ Sat, 08 Aug 2020 13:01:19: #2 alternative fragment length(s) may be 3,171,198 bps INFO @ Sat, 08 Aug 2020 13:01:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.10_model.r INFO @ Sat, 08 Aug 2020 13:01:19: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:01:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:01:20: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:01:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.10_peaks.xls INFO @ Sat, 08 Aug 2020 13:01:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.10_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:01:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.10_summits.bed INFO @ Sat, 08 Aug 2020 13:01:20: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (2 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 13:01:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 13:01:34: #1 read tag files... INFO @ Sat, 08 Aug 2020 13:01:34: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 13:01:40: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 13:01:47: 2000000 INFO @ Sat, 08 Aug 2020 13:01:49: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 13:01:49: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 13:01:49: #1 total tags in treatment: 876137 INFO @ Sat, 08 Aug 2020 13:01:49: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 13:01:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 13:01:49: #1 tags after filtering in treatment: 613487 INFO @ Sat, 08 Aug 2020 13:01:49: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 08 Aug 2020 13:01:49: #1 finished! INFO @ Sat, 08 Aug 2020 13:01:49: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 13:01:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 13:01:49: #2 number of paired peaks: 4970 INFO @ Sat, 08 Aug 2020 13:01:49: start model_add_line... INFO @ Sat, 08 Aug 2020 13:01:49: start X-correlation... INFO @ Sat, 08 Aug 2020 13:01:49: end of X-cor INFO @ Sat, 08 Aug 2020 13:01:49: #2 finished! INFO @ Sat, 08 Aug 2020 13:01:49: #2 predicted fragment length is 171 bps INFO @ Sat, 08 Aug 2020 13:01:49: #2 alternative fragment length(s) may be 3,171,198 bps INFO @ Sat, 08 Aug 2020 13:01:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.20_model.r INFO @ Sat, 08 Aug 2020 13:01:49: #3 Call peaks... INFO @ Sat, 08 Aug 2020 13:01:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 13:01:50: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 13:01:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.20_peaks.xls INFO @ Sat, 08 Aug 2020 13:01:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.20_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 13:01:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917466/SRX7917466.20_summits.bed INFO @ Sat, 08 Aug 2020 13:01:51: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling