Job ID = 8069591 SRX = SRX7917392 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T03:39:04 prefetch.2.10.7: 1) Downloading 'SRR11312939'... 2020-08-08T03:39:04 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:39:34 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:39:34 prefetch.2.10.7: 'SRR11312939' is valid 2020-08-08T03:39:34 prefetch.2.10.7: 1) 'SRR11312939' was downloaded successfully Read 1676819 spots for SRR11312939/SRR11312939.sra Written 1676819 spots for SRR11312939/SRR11312939.sra fastq に変換しました。 bowtie でマッピング中... Your job 8070023 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:24 1676819 reads; of these: 1676819 (100.00%) were paired; of these: 328659 (19.60%) aligned concordantly 0 times 1136226 (67.76%) aligned concordantly exactly 1 time 211934 (12.64%) aligned concordantly >1 times ---- 328659 pairs aligned concordantly 0 times; of these: 147508 (44.88%) aligned discordantly 1 time ---- 181151 pairs aligned 0 times concordantly or discordantly; of these: 362302 mates make up the pairs; of these: 164266 (45.34%) aligned 0 times 98764 (27.26%) aligned exactly 1 time 99272 (27.40%) aligned >1 times 95.10% overall alignment rate Time searching: 00:02:24 Overall time: 00:02:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 658257 / 1490446 = 0.4417 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:43:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:43:14: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:43:14: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:43:20: 1000000 INFO @ Sat, 08 Aug 2020 12:43:26: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:43:26: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:43:26: #1 total tags in treatment: 718008 INFO @ Sat, 08 Aug 2020 12:43:26: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:43:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:43:26: #1 tags after filtering in treatment: 537333 INFO @ Sat, 08 Aug 2020 12:43:26: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 08 Aug 2020 12:43:26: #1 finished! INFO @ Sat, 08 Aug 2020 12:43:26: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:43:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:43:26: #2 number of paired peaks: 3530 INFO @ Sat, 08 Aug 2020 12:43:26: start model_add_line... INFO @ Sat, 08 Aug 2020 12:43:26: start X-correlation... INFO @ Sat, 08 Aug 2020 12:43:26: end of X-cor INFO @ Sat, 08 Aug 2020 12:43:26: #2 finished! INFO @ Sat, 08 Aug 2020 12:43:26: #2 predicted fragment length is 199 bps INFO @ Sat, 08 Aug 2020 12:43:26: #2 alternative fragment length(s) may be 2,199,220 bps INFO @ Sat, 08 Aug 2020 12:43:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.05_model.r INFO @ Sat, 08 Aug 2020 12:43:26: #3 Call peaks... INFO @ Sat, 08 Aug 2020 12:43:26: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 12:43:27: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 12:43:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.05_peaks.xls INFO @ Sat, 08 Aug 2020 12:43:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.05_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 12:43:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.05_summits.bed INFO @ Sat, 08 Aug 2020 12:43:27: Done! pass1 - making usageList (1 chroms): 0 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:43:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:43:44: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:43:44: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:43:50: 1000000 INFO @ Sat, 08 Aug 2020 12:43:55: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:43:55: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:43:55: #1 total tags in treatment: 718008 INFO @ Sat, 08 Aug 2020 12:43:55: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:43:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:43:55: #1 tags after filtering in treatment: 537333 INFO @ Sat, 08 Aug 2020 12:43:55: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 08 Aug 2020 12:43:55: #1 finished! INFO @ Sat, 08 Aug 2020 12:43:55: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:43:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:43:55: #2 number of paired peaks: 3530 INFO @ Sat, 08 Aug 2020 12:43:55: start model_add_line... INFO @ Sat, 08 Aug 2020 12:43:55: start X-correlation... INFO @ Sat, 08 Aug 2020 12:43:55: end of X-cor INFO @ Sat, 08 Aug 2020 12:43:55: #2 finished! INFO @ Sat, 08 Aug 2020 12:43:55: #2 predicted fragment length is 199 bps INFO @ Sat, 08 Aug 2020 12:43:55: #2 alternative fragment length(s) may be 2,199,220 bps INFO @ Sat, 08 Aug 2020 12:43:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.10_model.r INFO @ Sat, 08 Aug 2020 12:43:55: #3 Call peaks... INFO @ Sat, 08 Aug 2020 12:43:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 12:43:56: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 12:43:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.10_peaks.xls INFO @ Sat, 08 Aug 2020 12:43:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.10_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 12:43:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.10_summits.bed INFO @ Sat, 08 Aug 2020 12:43:57: Done! pass1 - making usageList (1 chroms): 0 millis pass2 - checking and writing primary data (2 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:44:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:44:14: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:44:14: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 12:44:20: 1000000 BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 12:44:25: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:44:25: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:44:25: #1 total tags in treatment: 718008 INFO @ Sat, 08 Aug 2020 12:44:25: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:44:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:44:25: #1 tags after filtering in treatment: 537333 INFO @ Sat, 08 Aug 2020 12:44:25: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 08 Aug 2020 12:44:25: #1 finished! INFO @ Sat, 08 Aug 2020 12:44:25: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:44:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:44:26: #2 number of paired peaks: 3530 INFO @ Sat, 08 Aug 2020 12:44:26: start model_add_line... INFO @ Sat, 08 Aug 2020 12:44:26: start X-correlation... INFO @ Sat, 08 Aug 2020 12:44:26: end of X-cor INFO @ Sat, 08 Aug 2020 12:44:26: #2 finished! INFO @ Sat, 08 Aug 2020 12:44:26: #2 predicted fragment length is 199 bps INFO @ Sat, 08 Aug 2020 12:44:26: #2 alternative fragment length(s) may be 2,199,220 bps INFO @ Sat, 08 Aug 2020 12:44:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.20_model.r INFO @ Sat, 08 Aug 2020 12:44:26: #3 Call peaks... INFO @ Sat, 08 Aug 2020 12:44:26: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 12:44:27: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 12:44:27: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.20_peaks.xls INFO @ Sat, 08 Aug 2020 12:44:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.20_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 12:44:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917392/SRX7917392.20_summits.bed INFO @ Sat, 08 Aug 2020 12:44:27: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling