Job ID = 6626971 SRX = SRX7780066 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 29990947 spots for SRR11143821/SRR11143821.sra Written 29990947 spots for SRR11143821/SRR11143821.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627126 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:54 29990947 reads; of these: 29990947 (100.00%) were unpaired; of these: 2339073 (7.80%) aligned 0 times 20105131 (67.04%) aligned exactly 1 time 7546743 (25.16%) aligned >1 times 92.20% overall alignment rate Time searching: 00:08:54 Overall time: 00:08:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6425096 / 27651874 = 0.2324 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:22:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:22:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:22:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:22:18: 1000000 INFO @ Tue, 14 Jul 2020 09:22:22: 2000000 INFO @ Tue, 14 Jul 2020 09:22:27: 3000000 INFO @ Tue, 14 Jul 2020 09:22:31: 4000000 INFO @ Tue, 14 Jul 2020 09:22:36: 5000000 INFO @ Tue, 14 Jul 2020 09:22:40: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:22:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:22:43: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:22:43: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:22:45: 7000000 INFO @ Tue, 14 Jul 2020 09:22:49: 1000000 INFO @ Tue, 14 Jul 2020 09:22:50: 8000000 INFO @ Tue, 14 Jul 2020 09:22:54: 9000000 INFO @ Tue, 14 Jul 2020 09:22:56: 2000000 INFO @ Tue, 14 Jul 2020 09:22:59: 10000000 INFO @ Tue, 14 Jul 2020 09:23:03: 3000000 INFO @ Tue, 14 Jul 2020 09:23:04: 11000000 INFO @ Tue, 14 Jul 2020 09:23:08: 12000000 INFO @ Tue, 14 Jul 2020 09:23:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:23:13: 13000000 INFO @ Tue, 14 Jul 2020 09:23:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:23:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:23:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:23:17: 5000000 INFO @ Tue, 14 Jul 2020 09:23:18: 14000000 INFO @ Tue, 14 Jul 2020 09:23:21: 1000000 INFO @ Tue, 14 Jul 2020 09:23:23: 15000000 INFO @ Tue, 14 Jul 2020 09:23:26: 6000000 INFO @ Tue, 14 Jul 2020 09:23:28: 16000000 INFO @ Tue, 14 Jul 2020 09:23:30: 2000000 INFO @ Tue, 14 Jul 2020 09:23:32: 17000000 INFO @ Tue, 14 Jul 2020 09:23:34: 7000000 INFO @ Tue, 14 Jul 2020 09:23:37: 18000000 INFO @ Tue, 14 Jul 2020 09:23:38: 3000000 INFO @ Tue, 14 Jul 2020 09:23:42: 19000000 INFO @ Tue, 14 Jul 2020 09:23:43: 8000000 INFO @ Tue, 14 Jul 2020 09:23:46: 4000000 INFO @ Tue, 14 Jul 2020 09:23:47: 20000000 INFO @ Tue, 14 Jul 2020 09:23:51: 9000000 INFO @ Tue, 14 Jul 2020 09:23:51: 21000000 INFO @ Tue, 14 Jul 2020 09:23:53: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:23:53: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:23:53: #1 total tags in treatment: 21226778 INFO @ Tue, 14 Jul 2020 09:23:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:23:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:23:53: #1 tags after filtering in treatment: 21226778 INFO @ Tue, 14 Jul 2020 09:23:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:23:53: #1 finished! INFO @ Tue, 14 Jul 2020 09:23:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:23:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:23:54: 5000000 INFO @ Tue, 14 Jul 2020 09:23:54: #2 number of paired peaks: 5 WARNING @ Tue, 14 Jul 2020 09:23:54: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:23:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:23:58: 10000000 INFO @ Tue, 14 Jul 2020 09:24:02: 6000000 INFO @ Tue, 14 Jul 2020 09:24:06: 11000000 INFO @ Tue, 14 Jul 2020 09:24:10: 7000000 INFO @ Tue, 14 Jul 2020 09:24:14: 12000000 INFO @ Tue, 14 Jul 2020 09:24:17: 8000000 INFO @ Tue, 14 Jul 2020 09:24:21: 13000000 INFO @ Tue, 14 Jul 2020 09:24:24: 9000000 INFO @ Tue, 14 Jul 2020 09:24:28: 14000000 INFO @ Tue, 14 Jul 2020 09:24:32: 10000000 INFO @ Tue, 14 Jul 2020 09:24:36: 15000000 INFO @ Tue, 14 Jul 2020 09:24:39: 11000000 INFO @ Tue, 14 Jul 2020 09:24:43: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:24:46: 12000000 INFO @ Tue, 14 Jul 2020 09:24:51: 17000000 INFO @ Tue, 14 Jul 2020 09:24:54: 13000000 INFO @ Tue, 14 Jul 2020 09:24:58: 18000000 INFO @ Tue, 14 Jul 2020 09:25:01: 14000000 INFO @ Tue, 14 Jul 2020 09:25:06: 19000000 INFO @ Tue, 14 Jul 2020 09:25:09: 15000000 INFO @ Tue, 14 Jul 2020 09:25:13: 20000000 INFO @ Tue, 14 Jul 2020 09:25:16: 16000000 INFO @ Tue, 14 Jul 2020 09:25:21: 21000000 INFO @ Tue, 14 Jul 2020 09:25:22: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:25:22: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:25:22: #1 total tags in treatment: 21226778 INFO @ Tue, 14 Jul 2020 09:25:22: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:25:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:25:23: #1 tags after filtering in treatment: 21226778 INFO @ Tue, 14 Jul 2020 09:25:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:25:23: #1 finished! INFO @ Tue, 14 Jul 2020 09:25:23: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:25:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:25:24: 17000000 INFO @ Tue, 14 Jul 2020 09:25:24: #2 number of paired peaks: 5 WARNING @ Tue, 14 Jul 2020 09:25:24: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:25:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:25:31: 18000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:25:38: 19000000 INFO @ Tue, 14 Jul 2020 09:25:45: 20000000 INFO @ Tue, 14 Jul 2020 09:25:52: 21000000 INFO @ Tue, 14 Jul 2020 09:25:54: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:25:54: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:25:54: #1 total tags in treatment: 21226778 INFO @ Tue, 14 Jul 2020 09:25:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:25:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:25:54: #1 tags after filtering in treatment: 21226778 INFO @ Tue, 14 Jul 2020 09:25:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:25:54: #1 finished! INFO @ Tue, 14 Jul 2020 09:25:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:25:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:25:55: #2 number of paired peaks: 5 WARNING @ Tue, 14 Jul 2020 09:25:55: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:25:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780066/SRX7780066.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling