Job ID = 6626967 SRX = SRX7780063 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:03:51 prefetch.2.10.7: 1) Downloading 'SRR11143818'... 2020-07-14T00:03:51 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:04:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:04:53 prefetch.2.10.7: 1) 'SRR11143818' was downloaded successfully Read 27405760 spots for SRR11143818/SRR11143818.sra Written 27405760 spots for SRR11143818/SRR11143818.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627117 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:22 27405760 reads; of these: 27405760 (100.00%) were unpaired; of these: 4132519 (15.08%) aligned 0 times 16116460 (58.81%) aligned exactly 1 time 7156781 (26.11%) aligned >1 times 84.92% overall alignment rate Time searching: 00:08:22 Overall time: 00:08:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5354167 / 23273241 = 0.2301 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:19:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:19:53: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:19:53: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:19:59: 1000000 INFO @ Tue, 14 Jul 2020 09:20:04: 2000000 INFO @ Tue, 14 Jul 2020 09:20:10: 3000000 INFO @ Tue, 14 Jul 2020 09:20:15: 4000000 INFO @ Tue, 14 Jul 2020 09:20:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:20:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:20:23: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:20:23: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:20:26: 6000000 INFO @ Tue, 14 Jul 2020 09:20:29: 1000000 INFO @ Tue, 14 Jul 2020 09:20:32: 7000000 INFO @ Tue, 14 Jul 2020 09:20:35: 2000000 INFO @ Tue, 14 Jul 2020 09:20:38: 8000000 INFO @ Tue, 14 Jul 2020 09:20:41: 3000000 INFO @ Tue, 14 Jul 2020 09:20:44: 9000000 INFO @ Tue, 14 Jul 2020 09:20:47: 4000000 INFO @ Tue, 14 Jul 2020 09:20:50: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:20:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:20:53: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:20:53: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:20:53: 5000000 INFO @ Tue, 14 Jul 2020 09:20:56: 11000000 INFO @ Tue, 14 Jul 2020 09:21:00: 6000000 INFO @ Tue, 14 Jul 2020 09:21:00: 1000000 INFO @ Tue, 14 Jul 2020 09:21:03: 12000000 INFO @ Tue, 14 Jul 2020 09:21:06: 7000000 INFO @ Tue, 14 Jul 2020 09:21:07: 2000000 INFO @ Tue, 14 Jul 2020 09:21:10: 13000000 INFO @ Tue, 14 Jul 2020 09:21:13: 8000000 INFO @ Tue, 14 Jul 2020 09:21:13: 3000000 INFO @ Tue, 14 Jul 2020 09:21:16: 14000000 INFO @ Tue, 14 Jul 2020 09:21:20: 9000000 INFO @ Tue, 14 Jul 2020 09:21:20: 4000000 INFO @ Tue, 14 Jul 2020 09:21:23: 15000000 INFO @ Tue, 14 Jul 2020 09:21:26: 10000000 INFO @ Tue, 14 Jul 2020 09:21:27: 5000000 INFO @ Tue, 14 Jul 2020 09:21:30: 16000000 INFO @ Tue, 14 Jul 2020 09:21:33: 11000000 INFO @ Tue, 14 Jul 2020 09:21:33: 6000000 INFO @ Tue, 14 Jul 2020 09:21:36: 17000000 INFO @ Tue, 14 Jul 2020 09:21:39: 12000000 INFO @ Tue, 14 Jul 2020 09:21:40: 7000000 INFO @ Tue, 14 Jul 2020 09:21:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:21:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:21:43: #1 total tags in treatment: 17919074 INFO @ Tue, 14 Jul 2020 09:21:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:21:43: #1 tags after filtering in treatment: 17919074 INFO @ Tue, 14 Jul 2020 09:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:21:43: #1 finished! INFO @ Tue, 14 Jul 2020 09:21:43: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:21:44: #2 number of paired peaks: 16 WARNING @ Tue, 14 Jul 2020 09:21:44: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:21:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:21:46: 13000000 INFO @ Tue, 14 Jul 2020 09:21:47: 8000000 INFO @ Tue, 14 Jul 2020 09:21:53: 14000000 INFO @ Tue, 14 Jul 2020 09:21:53: 9000000 INFO @ Tue, 14 Jul 2020 09:21:59: 15000000 INFO @ Tue, 14 Jul 2020 09:22:00: 10000000 INFO @ Tue, 14 Jul 2020 09:22:05: 16000000 INFO @ Tue, 14 Jul 2020 09:22:07: 11000000 INFO @ Tue, 14 Jul 2020 09:22:12: 17000000 INFO @ Tue, 14 Jul 2020 09:22:13: 12000000 INFO @ Tue, 14 Jul 2020 09:22:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:22:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:22:18: #1 total tags in treatment: 17919074 INFO @ Tue, 14 Jul 2020 09:22:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:22:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:22:18: #1 tags after filtering in treatment: 17919074 INFO @ Tue, 14 Jul 2020 09:22:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:22:18: #1 finished! INFO @ Tue, 14 Jul 2020 09:22:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:22:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:22:19: #2 number of paired peaks: 16 WARNING @ Tue, 14 Jul 2020 09:22:19: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:22:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:22:20: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:22:26: 14000000 INFO @ Tue, 14 Jul 2020 09:22:32: 15000000 INFO @ Tue, 14 Jul 2020 09:22:38: 16000000 INFO @ Tue, 14 Jul 2020 09:22:45: 17000000 INFO @ Tue, 14 Jul 2020 09:22:50: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:22:50: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:22:50: #1 total tags in treatment: 17919074 INFO @ Tue, 14 Jul 2020 09:22:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:22:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:22:51: #1 tags after filtering in treatment: 17919074 INFO @ Tue, 14 Jul 2020 09:22:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:22:51: #1 finished! INFO @ Tue, 14 Jul 2020 09:22:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:22:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:22:52: #2 number of paired peaks: 16 WARNING @ Tue, 14 Jul 2020 09:22:52: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:22:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780063/SRX7780063.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。