Job ID = 6626962 SRX = SRX7780060 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:04:41 prefetch.2.10.7: 1) Downloading 'SRR11143815'... 2020-07-14T00:04:41 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:06:24 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:06:24 prefetch.2.10.7: 1) 'SRR11143815' was downloaded successfully Read 24844797 spots for SRR11143815/SRR11143815.sra Written 24844797 spots for SRR11143815/SRR11143815.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627125 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:24 24844797 reads; of these: 24844797 (100.00%) were unpaired; of these: 2524420 (10.16%) aligned 0 times 16207397 (65.23%) aligned exactly 1 time 6112980 (24.60%) aligned >1 times 89.84% overall alignment rate Time searching: 00:08:24 Overall time: 00:08:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4551146 / 22320377 = 0.2039 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:21:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:21:30: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:21:30: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:21:36: 1000000 INFO @ Tue, 14 Jul 2020 09:21:42: 2000000 INFO @ Tue, 14 Jul 2020 09:21:48: 3000000 INFO @ Tue, 14 Jul 2020 09:21:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:22:00: 5000000 INFO @ Tue, 14 Jul 2020 09:22:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:22:00: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:22:00: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:22:06: 6000000 INFO @ Tue, 14 Jul 2020 09:22:06: 1000000 INFO @ Tue, 14 Jul 2020 09:22:12: 2000000 INFO @ Tue, 14 Jul 2020 09:22:12: 7000000 INFO @ Tue, 14 Jul 2020 09:22:17: 3000000 INFO @ Tue, 14 Jul 2020 09:22:18: 8000000 INFO @ Tue, 14 Jul 2020 09:22:23: 4000000 INFO @ Tue, 14 Jul 2020 09:22:24: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:22:29: 5000000 INFO @ Tue, 14 Jul 2020 09:22:29: 10000000 INFO @ Tue, 14 Jul 2020 09:22:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:22:30: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:22:30: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:22:34: 6000000 INFO @ Tue, 14 Jul 2020 09:22:35: 11000000 INFO @ Tue, 14 Jul 2020 09:22:36: 1000000 INFO @ Tue, 14 Jul 2020 09:22:40: 7000000 INFO @ Tue, 14 Jul 2020 09:22:41: 12000000 INFO @ Tue, 14 Jul 2020 09:22:42: 2000000 INFO @ Tue, 14 Jul 2020 09:22:46: 8000000 INFO @ Tue, 14 Jul 2020 09:22:47: 13000000 INFO @ Tue, 14 Jul 2020 09:22:48: 3000000 INFO @ Tue, 14 Jul 2020 09:22:51: 9000000 INFO @ Tue, 14 Jul 2020 09:22:54: 4000000 INFO @ Tue, 14 Jul 2020 09:22:54: 14000000 INFO @ Tue, 14 Jul 2020 09:22:57: 10000000 INFO @ Tue, 14 Jul 2020 09:22:59: 5000000 INFO @ Tue, 14 Jul 2020 09:23:00: 15000000 INFO @ Tue, 14 Jul 2020 09:23:03: 11000000 INFO @ Tue, 14 Jul 2020 09:23:05: 6000000 INFO @ Tue, 14 Jul 2020 09:23:06: 16000000 INFO @ Tue, 14 Jul 2020 09:23:08: 12000000 INFO @ Tue, 14 Jul 2020 09:23:11: 7000000 INFO @ Tue, 14 Jul 2020 09:23:12: 17000000 INFO @ Tue, 14 Jul 2020 09:23:14: 13000000 INFO @ Tue, 14 Jul 2020 09:23:16: 8000000 INFO @ Tue, 14 Jul 2020 09:23:17: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:23:17: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:23:17: #1 total tags in treatment: 17769231 INFO @ Tue, 14 Jul 2020 09:23:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:23:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:23:17: #1 tags after filtering in treatment: 17769231 INFO @ Tue, 14 Jul 2020 09:23:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:23:17: #1 finished! INFO @ Tue, 14 Jul 2020 09:23:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:23:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:23:19: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:23:19: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:23:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:23:20: 14000000 INFO @ Tue, 14 Jul 2020 09:23:22: 9000000 INFO @ Tue, 14 Jul 2020 09:23:25: 15000000 INFO @ Tue, 14 Jul 2020 09:23:28: 10000000 INFO @ Tue, 14 Jul 2020 09:23:31: 16000000 INFO @ Tue, 14 Jul 2020 09:23:33: 11000000 INFO @ Tue, 14 Jul 2020 09:23:36: 17000000 INFO @ Tue, 14 Jul 2020 09:23:39: 12000000 INFO @ Tue, 14 Jul 2020 09:23:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:23:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:23:41: #1 total tags in treatment: 17769231 INFO @ Tue, 14 Jul 2020 09:23:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:23:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:23:41: #1 tags after filtering in treatment: 17769231 INFO @ Tue, 14 Jul 2020 09:23:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:23:41: #1 finished! INFO @ Tue, 14 Jul 2020 09:23:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:23:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:23:42: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:23:42: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:23:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:23:44: 13000000 INFO @ Tue, 14 Jul 2020 09:23:50: 14000000 INFO @ Tue, 14 Jul 2020 09:23:55: 15000000 INFO @ Tue, 14 Jul 2020 09:24:01: 16000000 INFO @ Tue, 14 Jul 2020 09:24:06: 17000000 INFO @ Tue, 14 Jul 2020 09:24:10: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:24:10: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:24:10: #1 total tags in treatment: 17769231 INFO @ Tue, 14 Jul 2020 09:24:10: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:24:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:24:11: #1 tags after filtering in treatment: 17769231 INFO @ Tue, 14 Jul 2020 09:24:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:24:11: #1 finished! INFO @ Tue, 14 Jul 2020 09:24:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:24:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:24:12: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:24:12: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:24:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780060/SRX7780060.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。