Job ID = 6626891 SRX = SRX7780048 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:57:34 prefetch.2.10.7: 1) Downloading 'SRR11143803'... 2020-07-13T23:57:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:58:52 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:58:53 prefetch.2.10.7: 'SRR11143803' is valid 2020-07-13T23:58:53 prefetch.2.10.7: 1) 'SRR11143803' was downloaded successfully Read 22205307 spots for SRR11143803/SRR11143803.sra Written 22205307 spots for SRR11143803/SRR11143803.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627088 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:33 22205307 reads; of these: 22205307 (100.00%) were unpaired; of these: 1708260 (7.69%) aligned 0 times 14950909 (67.33%) aligned exactly 1 time 5546138 (24.98%) aligned >1 times 92.31% overall alignment rate Time searching: 00:06:33 Overall time: 00:06:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3933003 / 20497047 = 0.1919 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:10:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:10:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:10:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:10:51: 1000000 INFO @ Tue, 14 Jul 2020 09:10:56: 2000000 INFO @ Tue, 14 Jul 2020 09:11:01: 3000000 INFO @ Tue, 14 Jul 2020 09:11:06: 4000000 INFO @ Tue, 14 Jul 2020 09:11:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:11:15: 6000000 INFO @ Tue, 14 Jul 2020 09:11:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:11:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:11:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:11:21: 7000000 INFO @ Tue, 14 Jul 2020 09:11:21: 1000000 INFO @ Tue, 14 Jul 2020 09:11:26: 8000000 INFO @ Tue, 14 Jul 2020 09:11:27: 2000000 INFO @ Tue, 14 Jul 2020 09:11:31: 9000000 INFO @ Tue, 14 Jul 2020 09:11:33: 3000000 INFO @ Tue, 14 Jul 2020 09:11:36: 10000000 INFO @ Tue, 14 Jul 2020 09:11:39: 4000000 INFO @ Tue, 14 Jul 2020 09:11:42: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:11:45: 5000000 INFO @ Tue, 14 Jul 2020 09:11:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:11:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:11:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:11:47: 12000000 INFO @ Tue, 14 Jul 2020 09:11:51: 6000000 INFO @ Tue, 14 Jul 2020 09:11:52: 1000000 INFO @ Tue, 14 Jul 2020 09:11:52: 13000000 INFO @ Tue, 14 Jul 2020 09:11:57: 7000000 INFO @ Tue, 14 Jul 2020 09:11:58: 2000000 INFO @ Tue, 14 Jul 2020 09:11:58: 14000000 INFO @ Tue, 14 Jul 2020 09:12:02: 8000000 INFO @ Tue, 14 Jul 2020 09:12:03: 15000000 INFO @ Tue, 14 Jul 2020 09:12:03: 3000000 INFO @ Tue, 14 Jul 2020 09:12:08: 16000000 INFO @ Tue, 14 Jul 2020 09:12:08: 9000000 INFO @ Tue, 14 Jul 2020 09:12:09: 4000000 INFO @ Tue, 14 Jul 2020 09:12:11: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:12:11: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:12:11: #1 total tags in treatment: 16564044 INFO @ Tue, 14 Jul 2020 09:12:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:12:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:12:12: #1 tags after filtering in treatment: 16564044 INFO @ Tue, 14 Jul 2020 09:12:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:12:12: #1 finished! INFO @ Tue, 14 Jul 2020 09:12:12: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:12:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:12:13: #2 number of paired peaks: 14 WARNING @ Tue, 14 Jul 2020 09:12:13: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:12:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:12:14: 10000000 INFO @ Tue, 14 Jul 2020 09:12:15: 5000000 INFO @ Tue, 14 Jul 2020 09:12:20: 11000000 INFO @ Tue, 14 Jul 2020 09:12:21: 6000000 INFO @ Tue, 14 Jul 2020 09:12:25: 12000000 INFO @ Tue, 14 Jul 2020 09:12:26: 7000000 INFO @ Tue, 14 Jul 2020 09:12:31: 13000000 INFO @ Tue, 14 Jul 2020 09:12:32: 8000000 INFO @ Tue, 14 Jul 2020 09:12:37: 14000000 INFO @ Tue, 14 Jul 2020 09:12:38: 9000000 INFO @ Tue, 14 Jul 2020 09:12:42: 15000000 INFO @ Tue, 14 Jul 2020 09:12:43: 10000000 INFO @ Tue, 14 Jul 2020 09:12:48: 16000000 INFO @ Tue, 14 Jul 2020 09:12:49: 11000000 INFO @ Tue, 14 Jul 2020 09:12:51: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:12:51: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:12:51: #1 total tags in treatment: 16564044 INFO @ Tue, 14 Jul 2020 09:12:51: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:12:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:12:51: #1 tags after filtering in treatment: 16564044 INFO @ Tue, 14 Jul 2020 09:12:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:12:51: #1 finished! INFO @ Tue, 14 Jul 2020 09:12:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:12:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:12:52: #2 number of paired peaks: 14 WARNING @ Tue, 14 Jul 2020 09:12:52: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:12:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:12:54: 12000000 INFO @ Tue, 14 Jul 2020 09:13:00: 13000000 INFO @ Tue, 14 Jul 2020 09:13:05: 14000000 INFO @ Tue, 14 Jul 2020 09:13:11: 15000000 INFO @ Tue, 14 Jul 2020 09:13:17: 16000000 INFO @ Tue, 14 Jul 2020 09:13:20: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:13:20: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:13:20: #1 total tags in treatment: 16564044 INFO @ Tue, 14 Jul 2020 09:13:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:13:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:13:20: #1 tags after filtering in treatment: 16564044 INFO @ Tue, 14 Jul 2020 09:13:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:13:20: #1 finished! INFO @ Tue, 14 Jul 2020 09:13:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:13:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:13:21: #2 number of paired peaks: 14 WARNING @ Tue, 14 Jul 2020 09:13:21: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:13:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780048/SRX7780048.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。