Job ID = 6626879 SRX = SRX7780047 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27180773 spots for SRR11143802/SRR11143802.sra Written 27180773 spots for SRR11143802/SRR11143802.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627085 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:20 27180773 reads; of these: 27180773 (100.00%) were unpaired; of these: 2349314 (8.64%) aligned 0 times 18469261 (67.95%) aligned exactly 1 time 6362198 (23.41%) aligned >1 times 91.36% overall alignment rate Time searching: 00:07:20 Overall time: 00:07:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5480421 / 24831459 = 0.2207 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:08:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:08:57: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:08:57: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:09:01: 1000000 INFO @ Tue, 14 Jul 2020 09:09:06: 2000000 INFO @ Tue, 14 Jul 2020 09:09:10: 3000000 INFO @ Tue, 14 Jul 2020 09:09:15: 4000000 INFO @ Tue, 14 Jul 2020 09:09:19: 5000000 INFO @ Tue, 14 Jul 2020 09:09:24: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:09:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:09:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:09:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:09:29: 7000000 INFO @ Tue, 14 Jul 2020 09:09:32: 1000000 INFO @ Tue, 14 Jul 2020 09:09:33: 8000000 INFO @ Tue, 14 Jul 2020 09:09:38: 2000000 INFO @ Tue, 14 Jul 2020 09:09:38: 9000000 INFO @ Tue, 14 Jul 2020 09:09:43: 10000000 INFO @ Tue, 14 Jul 2020 09:09:43: 3000000 INFO @ Tue, 14 Jul 2020 09:09:47: 11000000 INFO @ Tue, 14 Jul 2020 09:09:49: 4000000 INFO @ Tue, 14 Jul 2020 09:09:52: 12000000 INFO @ Tue, 14 Jul 2020 09:09:54: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:09:57: 13000000 INFO @ Tue, 14 Jul 2020 09:09:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:09:57: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:09:57: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:09:59: 6000000 INFO @ Tue, 14 Jul 2020 09:10:01: 14000000 INFO @ Tue, 14 Jul 2020 09:10:02: 1000000 INFO @ Tue, 14 Jul 2020 09:10:05: 7000000 INFO @ Tue, 14 Jul 2020 09:10:06: 15000000 INFO @ Tue, 14 Jul 2020 09:10:07: 2000000 INFO @ Tue, 14 Jul 2020 09:10:10: 8000000 INFO @ Tue, 14 Jul 2020 09:10:11: 16000000 INFO @ Tue, 14 Jul 2020 09:10:11: 3000000 INFO @ Tue, 14 Jul 2020 09:10:15: 9000000 INFO @ Tue, 14 Jul 2020 09:10:16: 17000000 INFO @ Tue, 14 Jul 2020 09:10:16: 4000000 INFO @ Tue, 14 Jul 2020 09:10:20: 10000000 INFO @ Tue, 14 Jul 2020 09:10:21: 18000000 INFO @ Tue, 14 Jul 2020 09:10:21: 5000000 INFO @ Tue, 14 Jul 2020 09:10:25: 19000000 INFO @ Tue, 14 Jul 2020 09:10:26: 6000000 INFO @ Tue, 14 Jul 2020 09:10:26: 11000000 INFO @ Tue, 14 Jul 2020 09:10:27: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:10:27: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:10:27: #1 total tags in treatment: 19351038 INFO @ Tue, 14 Jul 2020 09:10:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:10:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:10:27: #1 tags after filtering in treatment: 19351038 INFO @ Tue, 14 Jul 2020 09:10:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:10:27: #1 finished! INFO @ Tue, 14 Jul 2020 09:10:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:10:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:10:29: #2 number of paired peaks: 4 WARNING @ Tue, 14 Jul 2020 09:10:29: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:10:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:10:31: 7000000 INFO @ Tue, 14 Jul 2020 09:10:31: 12000000 INFO @ Tue, 14 Jul 2020 09:10:35: 8000000 INFO @ Tue, 14 Jul 2020 09:10:36: 13000000 INFO @ Tue, 14 Jul 2020 09:10:40: 9000000 INFO @ Tue, 14 Jul 2020 09:10:42: 14000000 INFO @ Tue, 14 Jul 2020 09:10:45: 10000000 INFO @ Tue, 14 Jul 2020 09:10:47: 15000000 INFO @ Tue, 14 Jul 2020 09:10:49: 11000000 INFO @ Tue, 14 Jul 2020 09:10:52: 16000000 INFO @ Tue, 14 Jul 2020 09:10:54: 12000000 INFO @ Tue, 14 Jul 2020 09:10:58: 17000000 INFO @ Tue, 14 Jul 2020 09:10:59: 13000000 INFO @ Tue, 14 Jul 2020 09:11:03: 18000000 INFO @ Tue, 14 Jul 2020 09:11:03: 14000000 INFO @ Tue, 14 Jul 2020 09:11:08: 15000000 INFO @ Tue, 14 Jul 2020 09:11:08: 19000000 INFO @ Tue, 14 Jul 2020 09:11:10: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:11:10: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:11:10: #1 total tags in treatment: 19351038 INFO @ Tue, 14 Jul 2020 09:11:10: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:11:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:11:11: #1 tags after filtering in treatment: 19351038 INFO @ Tue, 14 Jul 2020 09:11:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:11:11: #1 finished! INFO @ Tue, 14 Jul 2020 09:11:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:11:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:11:12: #2 number of paired peaks: 4 WARNING @ Tue, 14 Jul 2020 09:11:12: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:11:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:11:13: 16000000 INFO @ Tue, 14 Jul 2020 09:11:18: 17000000 INFO @ Tue, 14 Jul 2020 09:11:22: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:11:27: 19000000 INFO @ Tue, 14 Jul 2020 09:11:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:11:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:11:29: #1 total tags in treatment: 19351038 INFO @ Tue, 14 Jul 2020 09:11:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:11:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:11:29: #1 tags after filtering in treatment: 19351038 INFO @ Tue, 14 Jul 2020 09:11:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:11:29: #1 finished! INFO @ Tue, 14 Jul 2020 09:11:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:11:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:11:30: #2 number of paired peaks: 4 WARNING @ Tue, 14 Jul 2020 09:11:30: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:11:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780047/SRX7780047.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。