Job ID = 6626877 SRX = SRX7780046 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:54:04 prefetch.2.10.7: 1) Downloading 'SRR11143801'... 2020-07-13T23:54:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:55:33 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:55:33 prefetch.2.10.7: 1) 'SRR11143801' was downloaded successfully Read 25758396 spots for SRR11143801/SRR11143801.sra Written 25758396 spots for SRR11143801/SRR11143801.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627083 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:04 25758396 reads; of these: 25758396 (100.00%) were unpaired; of these: 2757907 (10.71%) aligned 0 times 16821910 (65.31%) aligned exactly 1 time 6178579 (23.99%) aligned >1 times 89.29% overall alignment rate Time searching: 00:07:04 Overall time: 00:07:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4705189 / 23000489 = 0.2046 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:08:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:08:30: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:08:30: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:08:37: 1000000 INFO @ Tue, 14 Jul 2020 09:08:43: 2000000 INFO @ Tue, 14 Jul 2020 09:08:49: 3000000 INFO @ Tue, 14 Jul 2020 09:08:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:09:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:09:00: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:09:00: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:09:01: 5000000 INFO @ Tue, 14 Jul 2020 09:09:06: 1000000 INFO @ Tue, 14 Jul 2020 09:09:07: 6000000 INFO @ Tue, 14 Jul 2020 09:09:12: 2000000 INFO @ Tue, 14 Jul 2020 09:09:12: 7000000 INFO @ Tue, 14 Jul 2020 09:09:18: 8000000 INFO @ Tue, 14 Jul 2020 09:09:18: 3000000 INFO @ Tue, 14 Jul 2020 09:09:24: 9000000 INFO @ Tue, 14 Jul 2020 09:09:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:09:30: 10000000 INFO @ Tue, 14 Jul 2020 09:09:30: 5000000 INFO @ Tue, 14 Jul 2020 09:09:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:09:30: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:09:30: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:09:37: 11000000 INFO @ Tue, 14 Jul 2020 09:09:37: 6000000 INFO @ Tue, 14 Jul 2020 09:09:38: 1000000 INFO @ Tue, 14 Jul 2020 09:09:44: 12000000 INFO @ Tue, 14 Jul 2020 09:09:44: 7000000 INFO @ Tue, 14 Jul 2020 09:09:45: 2000000 INFO @ Tue, 14 Jul 2020 09:09:50: 13000000 INFO @ Tue, 14 Jul 2020 09:09:51: 8000000 INFO @ Tue, 14 Jul 2020 09:09:52: 3000000 INFO @ Tue, 14 Jul 2020 09:09:57: 14000000 INFO @ Tue, 14 Jul 2020 09:09:58: 9000000 INFO @ Tue, 14 Jul 2020 09:09:59: 4000000 INFO @ Tue, 14 Jul 2020 09:10:04: 15000000 INFO @ Tue, 14 Jul 2020 09:10:04: 10000000 INFO @ Tue, 14 Jul 2020 09:10:06: 5000000 INFO @ Tue, 14 Jul 2020 09:10:11: 16000000 INFO @ Tue, 14 Jul 2020 09:10:11: 11000000 INFO @ Tue, 14 Jul 2020 09:10:12: 6000000 INFO @ Tue, 14 Jul 2020 09:10:17: 17000000 INFO @ Tue, 14 Jul 2020 09:10:18: 12000000 INFO @ Tue, 14 Jul 2020 09:10:19: 7000000 INFO @ Tue, 14 Jul 2020 09:10:24: 18000000 INFO @ Tue, 14 Jul 2020 09:10:24: 13000000 INFO @ Tue, 14 Jul 2020 09:10:26: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:10:26: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:10:26: #1 total tags in treatment: 18295300 INFO @ Tue, 14 Jul 2020 09:10:26: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:10:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:10:26: 8000000 INFO @ Tue, 14 Jul 2020 09:10:26: #1 tags after filtering in treatment: 18295300 INFO @ Tue, 14 Jul 2020 09:10:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:10:26: #1 finished! INFO @ Tue, 14 Jul 2020 09:10:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:10:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:10:27: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 09:10:27: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:10:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:10:31: 14000000 INFO @ Tue, 14 Jul 2020 09:10:32: 9000000 INFO @ Tue, 14 Jul 2020 09:10:37: 15000000 INFO @ Tue, 14 Jul 2020 09:10:39: 10000000 INFO @ Tue, 14 Jul 2020 09:10:44: 16000000 INFO @ Tue, 14 Jul 2020 09:10:46: 11000000 INFO @ Tue, 14 Jul 2020 09:10:50: 17000000 INFO @ Tue, 14 Jul 2020 09:10:52: 12000000 INFO @ Tue, 14 Jul 2020 09:10:57: 18000000 INFO @ Tue, 14 Jul 2020 09:10:59: 13000000 INFO @ Tue, 14 Jul 2020 09:10:59: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:10:59: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:10:59: #1 total tags in treatment: 18295300 INFO @ Tue, 14 Jul 2020 09:10:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:10:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:10:59: #1 tags after filtering in treatment: 18295300 INFO @ Tue, 14 Jul 2020 09:10:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:10:59: #1 finished! INFO @ Tue, 14 Jul 2020 09:10:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:10:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:11:00: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 09:11:00: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:11:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:11:04: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:11:10: 15000000 INFO @ Tue, 14 Jul 2020 09:11:16: 16000000 INFO @ Tue, 14 Jul 2020 09:11:22: 17000000 INFO @ Tue, 14 Jul 2020 09:11:27: 18000000 INFO @ Tue, 14 Jul 2020 09:11:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:11:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:11:29: #1 total tags in treatment: 18295300 INFO @ Tue, 14 Jul 2020 09:11:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:11:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:11:29: #1 tags after filtering in treatment: 18295300 INFO @ Tue, 14 Jul 2020 09:11:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:11:29: #1 finished! INFO @ Tue, 14 Jul 2020 09:11:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:11:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:11:30: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 09:11:30: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:11:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780046/SRX7780046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。