Job ID = 6626828 SRX = SRX7780042 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:51:04 prefetch.2.10.7: 1) Downloading 'SRR11143797'... 2020-07-13T23:51:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:51:57 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:51:57 prefetch.2.10.7: 1) 'SRR11143797' was downloaded successfully Read 25070454 spots for SRR11143797/SRR11143797.sra Written 25070454 spots for SRR11143797/SRR11143797.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627070 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:26 25070454 reads; of these: 25070454 (100.00%) were unpaired; of these: 2020559 (8.06%) aligned 0 times 17662038 (70.45%) aligned exactly 1 time 5387857 (21.49%) aligned >1 times 91.94% overall alignment rate Time searching: 00:06:26 Overall time: 00:06:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4775389 / 23049895 = 0.2072 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:04:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:04:22: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:04:22: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:04:28: 1000000 INFO @ Tue, 14 Jul 2020 09:04:34: 2000000 INFO @ Tue, 14 Jul 2020 09:04:40: 3000000 INFO @ Tue, 14 Jul 2020 09:04:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:04:51: 5000000 INFO @ Tue, 14 Jul 2020 09:04:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:04:52: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:04:52: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:04:57: 6000000 INFO @ Tue, 14 Jul 2020 09:04:59: 1000000 INFO @ Tue, 14 Jul 2020 09:05:03: 7000000 INFO @ Tue, 14 Jul 2020 09:05:05: 2000000 INFO @ Tue, 14 Jul 2020 09:05:10: 8000000 INFO @ Tue, 14 Jul 2020 09:05:11: 3000000 INFO @ Tue, 14 Jul 2020 09:05:16: 9000000 INFO @ Tue, 14 Jul 2020 09:05:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:05:22: 10000000 INFO @ Tue, 14 Jul 2020 09:05:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:05:22: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:05:22: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:05:24: 5000000 INFO @ Tue, 14 Jul 2020 09:05:28: 11000000 INFO @ Tue, 14 Jul 2020 09:05:29: 1000000 INFO @ Tue, 14 Jul 2020 09:05:30: 6000000 INFO @ Tue, 14 Jul 2020 09:05:35: 12000000 INFO @ Tue, 14 Jul 2020 09:05:36: 2000000 INFO @ Tue, 14 Jul 2020 09:05:37: 7000000 INFO @ Tue, 14 Jul 2020 09:05:42: 13000000 INFO @ Tue, 14 Jul 2020 09:05:43: 3000000 INFO @ Tue, 14 Jul 2020 09:05:44: 8000000 INFO @ Tue, 14 Jul 2020 09:05:48: 14000000 INFO @ Tue, 14 Jul 2020 09:05:50: 4000000 INFO @ Tue, 14 Jul 2020 09:05:51: 9000000 INFO @ Tue, 14 Jul 2020 09:05:55: 15000000 INFO @ Tue, 14 Jul 2020 09:05:57: 5000000 INFO @ Tue, 14 Jul 2020 09:05:58: 10000000 INFO @ Tue, 14 Jul 2020 09:06:02: 16000000 INFO @ Tue, 14 Jul 2020 09:06:04: 6000000 INFO @ Tue, 14 Jul 2020 09:06:05: 11000000 INFO @ Tue, 14 Jul 2020 09:06:09: 17000000 INFO @ Tue, 14 Jul 2020 09:06:11: 7000000 INFO @ Tue, 14 Jul 2020 09:06:12: 12000000 INFO @ Tue, 14 Jul 2020 09:06:15: 18000000 INFO @ Tue, 14 Jul 2020 09:06:17: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:06:17: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:06:17: #1 total tags in treatment: 18274506 INFO @ Tue, 14 Jul 2020 09:06:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:06:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:06:17: 8000000 INFO @ Tue, 14 Jul 2020 09:06:18: #1 tags after filtering in treatment: 18274506 INFO @ Tue, 14 Jul 2020 09:06:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:06:18: #1 finished! INFO @ Tue, 14 Jul 2020 09:06:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:06:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:06:18: 13000000 INFO @ Tue, 14 Jul 2020 09:06:19: #2 number of paired peaks: 9 WARNING @ Tue, 14 Jul 2020 09:06:19: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:06:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:06:24: 9000000 INFO @ Tue, 14 Jul 2020 09:06:25: 14000000 INFO @ Tue, 14 Jul 2020 09:06:30: 10000000 INFO @ Tue, 14 Jul 2020 09:06:31: 15000000 INFO @ Tue, 14 Jul 2020 09:06:37: 11000000 INFO @ Tue, 14 Jul 2020 09:06:38: 16000000 INFO @ Tue, 14 Jul 2020 09:06:43: 12000000 INFO @ Tue, 14 Jul 2020 09:06:44: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:06:50: 13000000 INFO @ Tue, 14 Jul 2020 09:06:51: 18000000 INFO @ Tue, 14 Jul 2020 09:06:53: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:06:53: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:06:53: #1 total tags in treatment: 18274506 INFO @ Tue, 14 Jul 2020 09:06:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:06:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:06:53: #1 tags after filtering in treatment: 18274506 INFO @ Tue, 14 Jul 2020 09:06:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:06:53: #1 finished! INFO @ Tue, 14 Jul 2020 09:06:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:06:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:06:54: #2 number of paired peaks: 9 WARNING @ Tue, 14 Jul 2020 09:06:54: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:06:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:06:56: 14000000 INFO @ Tue, 14 Jul 2020 09:07:02: 15000000 INFO @ Tue, 14 Jul 2020 09:07:09: 16000000 INFO @ Tue, 14 Jul 2020 09:07:15: 17000000 INFO @ Tue, 14 Jul 2020 09:07:21: 18000000 INFO @ Tue, 14 Jul 2020 09:07:22: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:07:22: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:07:22: #1 total tags in treatment: 18274506 INFO @ Tue, 14 Jul 2020 09:07:22: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:07:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:07:23: #1 tags after filtering in treatment: 18274506 INFO @ Tue, 14 Jul 2020 09:07:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:07:23: #1 finished! INFO @ Tue, 14 Jul 2020 09:07:23: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:07:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:07:24: #2 number of paired peaks: 9 WARNING @ Tue, 14 Jul 2020 09:07:24: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:07:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780042/SRX7780042.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。