Job ID = 6626781 SRX = SRX7780036 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:45:21 prefetch.2.10.7: 1) Downloading 'SRR11143791'... 2020-07-13T23:45:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:46:56 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:46:56 prefetch.2.10.7: 1) 'SRR11143791' was downloaded successfully Read 22791353 spots for SRR11143791/SRR11143791.sra Written 22791353 spots for SRR11143791/SRR11143791.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627055 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:51 22791353 reads; of these: 22791353 (100.00%) were unpaired; of these: 1851858 (8.13%) aligned 0 times 15460323 (67.83%) aligned exactly 1 time 5479172 (24.04%) aligned >1 times 91.87% overall alignment rate Time searching: 00:07:51 Overall time: 00:07:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3975042 / 20939495 = 0.1898 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:00:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:00:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:00:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:00:15: 1000000 INFO @ Tue, 14 Jul 2020 09:00:20: 2000000 INFO @ Tue, 14 Jul 2020 09:00:26: 3000000 INFO @ Tue, 14 Jul 2020 09:00:31: 4000000 INFO @ Tue, 14 Jul 2020 09:00:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:00:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:00:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:00:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:00:43: 6000000 INFO @ Tue, 14 Jul 2020 09:00:47: 1000000 INFO @ Tue, 14 Jul 2020 09:00:49: 7000000 INFO @ Tue, 14 Jul 2020 09:00:54: 2000000 INFO @ Tue, 14 Jul 2020 09:00:56: 8000000 INFO @ Tue, 14 Jul 2020 09:01:01: 3000000 INFO @ Tue, 14 Jul 2020 09:01:02: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:01:09: 4000000 INFO @ Tue, 14 Jul 2020 09:01:09: 10000000 INFO @ Tue, 14 Jul 2020 09:01:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:01:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:01:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:01:15: 11000000 INFO @ Tue, 14 Jul 2020 09:01:16: 5000000 INFO @ Tue, 14 Jul 2020 09:01:17: 1000000 INFO @ Tue, 14 Jul 2020 09:01:22: 12000000 INFO @ Tue, 14 Jul 2020 09:01:24: 6000000 INFO @ Tue, 14 Jul 2020 09:01:25: 2000000 INFO @ Tue, 14 Jul 2020 09:01:29: 13000000 INFO @ Tue, 14 Jul 2020 09:01:31: 7000000 INFO @ Tue, 14 Jul 2020 09:01:34: 3000000 INFO @ Tue, 14 Jul 2020 09:01:35: 14000000 INFO @ Tue, 14 Jul 2020 09:01:39: 8000000 INFO @ Tue, 14 Jul 2020 09:01:42: 15000000 INFO @ Tue, 14 Jul 2020 09:01:44: 4000000 INFO @ Tue, 14 Jul 2020 09:01:47: 9000000 INFO @ Tue, 14 Jul 2020 09:01:49: 16000000 INFO @ Tue, 14 Jul 2020 09:01:54: 10000000 INFO @ Tue, 14 Jul 2020 09:01:54: 5000000 INFO @ Tue, 14 Jul 2020 09:01:55: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:01:55: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:01:55: #1 total tags in treatment: 16964453 INFO @ Tue, 14 Jul 2020 09:01:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:01:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:01:56: #1 tags after filtering in treatment: 16964453 INFO @ Tue, 14 Jul 2020 09:01:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:01:56: #1 finished! INFO @ Tue, 14 Jul 2020 09:01:56: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:01:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:01:57: #2 number of paired peaks: 14 WARNING @ Tue, 14 Jul 2020 09:01:57: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:01:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:02:01: 11000000 INFO @ Tue, 14 Jul 2020 09:02:04: 6000000 INFO @ Tue, 14 Jul 2020 09:02:09: 12000000 INFO @ Tue, 14 Jul 2020 09:02:14: 7000000 INFO @ Tue, 14 Jul 2020 09:02:16: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:02:24: 8000000 INFO @ Tue, 14 Jul 2020 09:02:24: 14000000 INFO @ Tue, 14 Jul 2020 09:02:31: 15000000 INFO @ Tue, 14 Jul 2020 09:02:33: 9000000 INFO @ Tue, 14 Jul 2020 09:02:39: 16000000 INFO @ Tue, 14 Jul 2020 09:02:43: 10000000 INFO @ Tue, 14 Jul 2020 09:02:46: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:02:46: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:02:46: #1 total tags in treatment: 16964453 INFO @ Tue, 14 Jul 2020 09:02:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:02:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:02:46: #1 tags after filtering in treatment: 16964453 INFO @ Tue, 14 Jul 2020 09:02:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:02:46: #1 finished! INFO @ Tue, 14 Jul 2020 09:02:46: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:02:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:02:47: #2 number of paired peaks: 14 WARNING @ Tue, 14 Jul 2020 09:02:47: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:02:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:02:53: 11000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:03:03: 12000000 INFO @ Tue, 14 Jul 2020 09:03:12: 13000000 INFO @ Tue, 14 Jul 2020 09:03:22: 14000000 INFO @ Tue, 14 Jul 2020 09:03:32: 15000000 INFO @ Tue, 14 Jul 2020 09:03:41: 16000000 INFO @ Tue, 14 Jul 2020 09:03:50: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:03:50: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:03:50: #1 total tags in treatment: 16964453 INFO @ Tue, 14 Jul 2020 09:03:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:03:51: #1 tags after filtering in treatment: 16964453 INFO @ Tue, 14 Jul 2020 09:03:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:03:51: #1 finished! INFO @ Tue, 14 Jul 2020 09:03:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:03:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:03:52: #2 number of paired peaks: 14 WARNING @ Tue, 14 Jul 2020 09:03:52: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:03:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780036/SRX7780036.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling