Job ID = 6626774 SRX = SRX7780034 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:42:36 prefetch.2.10.7: 1) Downloading 'SRR11143789'... 2020-07-13T23:42:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:44:17 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:44:18 prefetch.2.10.7: 'SRR11143789' is valid 2020-07-13T23:44:18 prefetch.2.10.7: 1) 'SRR11143789' was downloaded successfully Read 18837731 spots for SRR11143789/SRR11143789.sra Written 18837731 spots for SRR11143789/SRR11143789.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627042 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 18837731 reads; of these: 18837731 (100.00%) were unpaired; of these: 1432770 (7.61%) aligned 0 times 12620796 (67.00%) aligned exactly 1 time 4784165 (25.40%) aligned >1 times 92.39% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2903639 / 17404961 = 0.1668 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:55:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:55:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:55:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:55:32: 1000000 INFO @ Tue, 14 Jul 2020 08:55:38: 2000000 INFO @ Tue, 14 Jul 2020 08:55:43: 3000000 INFO @ Tue, 14 Jul 2020 08:55:49: 4000000 INFO @ Tue, 14 Jul 2020 08:55:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:55:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:55:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:55:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:56:00: 6000000 INFO @ Tue, 14 Jul 2020 08:56:03: 1000000 INFO @ Tue, 14 Jul 2020 08:56:06: 7000000 INFO @ Tue, 14 Jul 2020 08:56:09: 2000000 INFO @ Tue, 14 Jul 2020 08:56:12: 8000000 INFO @ Tue, 14 Jul 2020 08:56:15: 3000000 INFO @ Tue, 14 Jul 2020 08:56:19: 9000000 INFO @ Tue, 14 Jul 2020 08:56:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:56:25: 10000000 INFO @ Tue, 14 Jul 2020 08:56:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:56:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:56:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:56:27: 5000000 INFO @ Tue, 14 Jul 2020 08:56:31: 11000000 INFO @ Tue, 14 Jul 2020 08:56:33: 1000000 INFO @ Tue, 14 Jul 2020 08:56:34: 6000000 INFO @ Tue, 14 Jul 2020 08:56:38: 12000000 INFO @ Tue, 14 Jul 2020 08:56:39: 2000000 INFO @ Tue, 14 Jul 2020 08:56:40: 7000000 INFO @ Tue, 14 Jul 2020 08:56:45: 13000000 INFO @ Tue, 14 Jul 2020 08:56:46: 3000000 INFO @ Tue, 14 Jul 2020 08:56:47: 8000000 INFO @ Tue, 14 Jul 2020 08:56:51: 14000000 INFO @ Tue, 14 Jul 2020 08:56:53: 4000000 INFO @ Tue, 14 Jul 2020 08:56:54: 9000000 INFO @ Tue, 14 Jul 2020 08:56:55: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:56:55: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:56:55: #1 total tags in treatment: 14501322 INFO @ Tue, 14 Jul 2020 08:56:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:56:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:56:55: #1 tags after filtering in treatment: 14501322 INFO @ Tue, 14 Jul 2020 08:56:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:56:55: #1 finished! INFO @ Tue, 14 Jul 2020 08:56:55: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:56:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:56:56: #2 number of paired peaks: 28 WARNING @ Tue, 14 Jul 2020 08:56:56: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:56:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:56:59: 5000000 INFO @ Tue, 14 Jul 2020 08:57:00: 10000000 INFO @ Tue, 14 Jul 2020 08:57:06: 6000000 INFO @ Tue, 14 Jul 2020 08:57:06: 11000000 INFO @ Tue, 14 Jul 2020 08:57:13: 7000000 INFO @ Tue, 14 Jul 2020 08:57:13: 12000000 INFO @ Tue, 14 Jul 2020 08:57:19: 8000000 INFO @ Tue, 14 Jul 2020 08:57:19: 13000000 INFO @ Tue, 14 Jul 2020 08:57:25: 9000000 INFO @ Tue, 14 Jul 2020 08:57:25: 14000000 INFO @ Tue, 14 Jul 2020 08:57:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:57:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:57:29: #1 total tags in treatment: 14501322 INFO @ Tue, 14 Jul 2020 08:57:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:29: #1 tags after filtering in treatment: 14501322 INFO @ Tue, 14 Jul 2020 08:57:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:57:29: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:57:30: #2 number of paired peaks: 28 WARNING @ Tue, 14 Jul 2020 08:57:30: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:57:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:57:32: 10000000 INFO @ Tue, 14 Jul 2020 08:57:38: 11000000 INFO @ Tue, 14 Jul 2020 08:57:44: 12000000 INFO @ Tue, 14 Jul 2020 08:57:50: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:57:55: 14000000 INFO @ Tue, 14 Jul 2020 08:57:59: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:57:59: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:57:59: #1 total tags in treatment: 14501322 INFO @ Tue, 14 Jul 2020 08:57:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:59: #1 tags after filtering in treatment: 14501322 INFO @ Tue, 14 Jul 2020 08:57:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:57:59: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:58:00: #2 number of paired peaks: 28 WARNING @ Tue, 14 Jul 2020 08:58:00: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:58:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780034/SRX7780034.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。