Job ID = 6626772 SRX = SRX7780032 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:41:46 prefetch.2.10.7: 1) Downloading 'SRR11143787'... 2020-07-13T23:41:46 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:42:41 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:42:42 prefetch.2.10.7: 'SRR11143787' is valid 2020-07-13T23:42:42 prefetch.2.10.7: 1) 'SRR11143787' was downloaded successfully Read 13531138 spots for SRR11143787/SRR11143787.sra Written 13531138 spots for SRR11143787/SRR11143787.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627036 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:58 13531138 reads; of these: 13531138 (100.00%) were unpaired; of these: 1480507 (10.94%) aligned 0 times 8674015 (64.10%) aligned exactly 1 time 3376616 (24.95%) aligned >1 times 89.06% overall alignment rate Time searching: 00:03:58 Overall time: 00:03:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1701004 / 12050631 = 0.1412 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:50:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:50:17: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:50:17: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:50:22: 1000000 INFO @ Tue, 14 Jul 2020 08:50:27: 2000000 INFO @ Tue, 14 Jul 2020 08:50:33: 3000000 INFO @ Tue, 14 Jul 2020 08:50:38: 4000000 INFO @ Tue, 14 Jul 2020 08:50:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:50:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:50:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:50:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:50:48: 6000000 INFO @ Tue, 14 Jul 2020 08:50:52: 1000000 INFO @ Tue, 14 Jul 2020 08:50:54: 7000000 INFO @ Tue, 14 Jul 2020 08:50:58: 2000000 INFO @ Tue, 14 Jul 2020 08:50:59: 8000000 INFO @ Tue, 14 Jul 2020 08:51:03: 3000000 INFO @ Tue, 14 Jul 2020 08:51:05: 9000000 INFO @ Tue, 14 Jul 2020 08:51:09: 4000000 INFO @ Tue, 14 Jul 2020 08:51:11: 10000000 INFO @ Tue, 14 Jul 2020 08:51:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:51:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:51:13: #1 total tags in treatment: 10349627 INFO @ Tue, 14 Jul 2020 08:51:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:51:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:51:13: #1 tags after filtering in treatment: 10349627 INFO @ Tue, 14 Jul 2020 08:51:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:51:13: #1 finished! INFO @ Tue, 14 Jul 2020 08:51:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:51:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:51:14: #2 number of paired peaks: 75 WARNING @ Tue, 14 Jul 2020 08:51:14: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:51:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:51:15: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:51:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:51:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:51:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:51:20: 6000000 INFO @ Tue, 14 Jul 2020 08:51:22: 1000000 INFO @ Tue, 14 Jul 2020 08:51:26: 7000000 INFO @ Tue, 14 Jul 2020 08:51:28: 2000000 INFO @ Tue, 14 Jul 2020 08:51:32: 8000000 INFO @ Tue, 14 Jul 2020 08:51:33: 3000000 INFO @ Tue, 14 Jul 2020 08:51:37: 9000000 INFO @ Tue, 14 Jul 2020 08:51:39: 4000000 INFO @ Tue, 14 Jul 2020 08:51:43: 10000000 INFO @ Tue, 14 Jul 2020 08:51:45: 5000000 INFO @ Tue, 14 Jul 2020 08:51:45: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:51:45: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:51:45: #1 total tags in treatment: 10349627 INFO @ Tue, 14 Jul 2020 08:51:45: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:51:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:51:45: #1 tags after filtering in treatment: 10349627 INFO @ Tue, 14 Jul 2020 08:51:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:51:45: #1 finished! INFO @ Tue, 14 Jul 2020 08:51:45: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:51:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:51:46: #2 number of paired peaks: 75 WARNING @ Tue, 14 Jul 2020 08:51:46: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:51:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:51:50: 6000000 INFO @ Tue, 14 Jul 2020 08:51:56: 7000000 INFO @ Tue, 14 Jul 2020 08:52:02: 8000000 INFO @ Tue, 14 Jul 2020 08:52:07: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:52:13: 10000000 INFO @ Tue, 14 Jul 2020 08:52:15: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:52:15: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:52:15: #1 total tags in treatment: 10349627 INFO @ Tue, 14 Jul 2020 08:52:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:52:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:52:15: #1 tags after filtering in treatment: 10349627 INFO @ Tue, 14 Jul 2020 08:52:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:52:15: #1 finished! INFO @ Tue, 14 Jul 2020 08:52:15: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:52:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:52:16: #2 number of paired peaks: 75 WARNING @ Tue, 14 Jul 2020 08:52:16: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:52:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780032/SRX7780032.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。