Job ID = 6626771 SRX = SRX7780031 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14563567 spots for SRR11143786/SRR11143786.sra Written 14563567 spots for SRR11143786/SRR11143786.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627031 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:15 14563567 reads; of these: 14563567 (100.00%) were unpaired; of these: 701210 (4.81%) aligned 0 times 11378667 (78.13%) aligned exactly 1 time 2483690 (17.05%) aligned >1 times 95.19% overall alignment rate Time searching: 00:04:15 Overall time: 00:04:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2467590 / 13862357 = 0.1780 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:49:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:49:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:49:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:49:20: 1000000 INFO @ Tue, 14 Jul 2020 08:49:25: 2000000 INFO @ Tue, 14 Jul 2020 08:49:30: 3000000 INFO @ Tue, 14 Jul 2020 08:49:35: 4000000 INFO @ Tue, 14 Jul 2020 08:49:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:49:44: 6000000 INFO @ Tue, 14 Jul 2020 08:49:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:49:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:49:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:49:49: 7000000 INFO @ Tue, 14 Jul 2020 08:49:50: 1000000 INFO @ Tue, 14 Jul 2020 08:49:54: 8000000 INFO @ Tue, 14 Jul 2020 08:49:55: 2000000 INFO @ Tue, 14 Jul 2020 08:49:58: 9000000 INFO @ Tue, 14 Jul 2020 08:50:00: 3000000 INFO @ Tue, 14 Jul 2020 08:50:03: 10000000 INFO @ Tue, 14 Jul 2020 08:50:05: 4000000 INFO @ Tue, 14 Jul 2020 08:50:08: 11000000 INFO @ Tue, 14 Jul 2020 08:50:09: 5000000 INFO @ Tue, 14 Jul 2020 08:50:10: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:50:10: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:50:10: #1 total tags in treatment: 11394767 INFO @ Tue, 14 Jul 2020 08:50:10: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:50:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:50:10: #1 tags after filtering in treatment: 11394767 INFO @ Tue, 14 Jul 2020 08:50:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:50:10: #1 finished! INFO @ Tue, 14 Jul 2020 08:50:10: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:50:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:50:11: #2 number of paired peaks: 24 WARNING @ Tue, 14 Jul 2020 08:50:11: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:50:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:50:14: 6000000 INFO @ Tue, 14 Jul 2020 08:50:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:50:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:50:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:50:19: 7000000 INFO @ Tue, 14 Jul 2020 08:50:20: 1000000 INFO @ Tue, 14 Jul 2020 08:50:24: 8000000 INFO @ Tue, 14 Jul 2020 08:50:25: 2000000 INFO @ Tue, 14 Jul 2020 08:50:29: 9000000 INFO @ Tue, 14 Jul 2020 08:50:30: 3000000 INFO @ Tue, 14 Jul 2020 08:50:34: 10000000 INFO @ Tue, 14 Jul 2020 08:50:35: 4000000 INFO @ Tue, 14 Jul 2020 08:50:39: 11000000 INFO @ Tue, 14 Jul 2020 08:50:40: 5000000 INFO @ Tue, 14 Jul 2020 08:50:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:50:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:50:41: #1 total tags in treatment: 11394767 INFO @ Tue, 14 Jul 2020 08:50:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:50:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:50:41: #1 tags after filtering in treatment: 11394767 INFO @ Tue, 14 Jul 2020 08:50:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:50:41: #1 finished! INFO @ Tue, 14 Jul 2020 08:50:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:50:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:50:41: #2 number of paired peaks: 24 WARNING @ Tue, 14 Jul 2020 08:50:41: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:50:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:50:45: 6000000 INFO @ Tue, 14 Jul 2020 08:50:50: 7000000 INFO @ Tue, 14 Jul 2020 08:50:54: 8000000 INFO @ Tue, 14 Jul 2020 08:50:59: 9000000 INFO @ Tue, 14 Jul 2020 08:51:04: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:51:09: 11000000 INFO @ Tue, 14 Jul 2020 08:51:10: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:51:10: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:51:10: #1 total tags in treatment: 11394767 INFO @ Tue, 14 Jul 2020 08:51:10: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:51:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:51:11: #1 tags after filtering in treatment: 11394767 INFO @ Tue, 14 Jul 2020 08:51:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:51:11: #1 finished! INFO @ Tue, 14 Jul 2020 08:51:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:51:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:51:11: #2 number of paired peaks: 24 WARNING @ Tue, 14 Jul 2020 08:51:11: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:51:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780031/SRX7780031.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。