Job ID = 10166252 SRX = SRX7723722 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26142343 spots for SRR11084678/SRR11084678.sra Written 26142343 spots for SRR11084678/SRR11084678.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166634 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:26 26142343 reads; of these: 26142343 (100.00%) were unpaired; of these: 5349135 (20.46%) aligned 0 times 17697145 (67.70%) aligned exactly 1 time 3096063 (11.84%) aligned >1 times 79.54% overall alignment rate Time searching: 00:08:26 Overall time: 00:08:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5947832 / 20793208 = 0.2860 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:58:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:58:08: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:58:08: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:58:13: 1000000 INFO @ Thu, 08 Oct 2020 20:58:18: 2000000 INFO @ Thu, 08 Oct 2020 20:58:23: 3000000 INFO @ Thu, 08 Oct 2020 20:58:28: 4000000 INFO @ Thu, 08 Oct 2020 20:58:33: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:58:38: 6000000 INFO @ Thu, 08 Oct 2020 20:58:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:58:38: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:58:38: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:58:42: 7000000 INFO @ Thu, 08 Oct 2020 20:58:43: 1000000 INFO @ Thu, 08 Oct 2020 20:58:47: 8000000 INFO @ Thu, 08 Oct 2020 20:58:48: 2000000 INFO @ Thu, 08 Oct 2020 20:58:52: 9000000 INFO @ Thu, 08 Oct 2020 20:58:53: 3000000 INFO @ Thu, 08 Oct 2020 20:58:57: 10000000 INFO @ Thu, 08 Oct 2020 20:58:58: 4000000 INFO @ Thu, 08 Oct 2020 20:59:02: 11000000 INFO @ Thu, 08 Oct 2020 20:59:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:59:07: 12000000 INFO @ Thu, 08 Oct 2020 20:59:08: 6000000 INFO @ Thu, 08 Oct 2020 20:59:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:59:08: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:59:08: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:59:12: 13000000 INFO @ Thu, 08 Oct 2020 20:59:13: 7000000 INFO @ Thu, 08 Oct 2020 20:59:13: 1000000 INFO @ Thu, 08 Oct 2020 20:59:17: 14000000 INFO @ Thu, 08 Oct 2020 20:59:18: 8000000 INFO @ Thu, 08 Oct 2020 20:59:18: 2000000 INFO @ Thu, 08 Oct 2020 20:59:21: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:59:21: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:59:21: #1 total tags in treatment: 14845376 INFO @ Thu, 08 Oct 2020 20:59:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:59:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:59:22: #1 tags after filtering in treatment: 14845376 INFO @ Thu, 08 Oct 2020 20:59:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:59:22: #1 finished! INFO @ Thu, 08 Oct 2020 20:59:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:59:22: #2 number of paired peaks: 61 WARNING @ Thu, 08 Oct 2020 20:59:22: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:59:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:59:23: 9000000 INFO @ Thu, 08 Oct 2020 20:59:23: 3000000 INFO @ Thu, 08 Oct 2020 20:59:28: 10000000 INFO @ Thu, 08 Oct 2020 20:59:28: 4000000 INFO @ Thu, 08 Oct 2020 20:59:32: 11000000 INFO @ Thu, 08 Oct 2020 20:59:33: 5000000 INFO @ Thu, 08 Oct 2020 20:59:37: 12000000 INFO @ Thu, 08 Oct 2020 20:59:38: 6000000 INFO @ Thu, 08 Oct 2020 20:59:42: 13000000 INFO @ Thu, 08 Oct 2020 20:59:43: 7000000 INFO @ Thu, 08 Oct 2020 20:59:47: 14000000 INFO @ Thu, 08 Oct 2020 20:59:48: 8000000 INFO @ Thu, 08 Oct 2020 20:59:52: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:59:52: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:59:52: #1 total tags in treatment: 14845376 INFO @ Thu, 08 Oct 2020 20:59:52: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:59:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:59:52: #1 tags after filtering in treatment: 14845376 INFO @ Thu, 08 Oct 2020 20:59:52: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:59:52: #1 finished! INFO @ Thu, 08 Oct 2020 20:59:52: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:59:52: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:59:52: 9000000 INFO @ Thu, 08 Oct 2020 20:59:53: #2 number of paired peaks: 61 WARNING @ Thu, 08 Oct 2020 20:59:53: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:59:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:59:57: 10000000 INFO @ Thu, 08 Oct 2020 21:00:02: 11000000 INFO @ Thu, 08 Oct 2020 21:00:07: 12000000 INFO @ Thu, 08 Oct 2020 21:00:12: 13000000 INFO @ Thu, 08 Oct 2020 21:00:17: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:00:21: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:00:21: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:00:21: #1 total tags in treatment: 14845376 INFO @ Thu, 08 Oct 2020 21:00:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:00:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:00:21: #1 tags after filtering in treatment: 14845376 INFO @ Thu, 08 Oct 2020 21:00:21: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:00:21: #1 finished! INFO @ Thu, 08 Oct 2020 21:00:21: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:00:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:00:22: #2 number of paired peaks: 61 WARNING @ Thu, 08 Oct 2020 21:00:22: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:00:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723722/SRX7723722.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。