Job ID = 10166233 SRX = SRX7723718 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 28473101 spots for SRR11084674/SRR11084674.sra Written 28473101 spots for SRR11084674/SRR11084674.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166632 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:05 28473101 reads; of these: 28473101 (100.00%) were unpaired; of these: 2771205 (9.73%) aligned 0 times 20322501 (71.37%) aligned exactly 1 time 5379395 (18.89%) aligned >1 times 90.27% overall alignment rate Time searching: 00:10:06 Overall time: 00:10:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2928197 / 25701896 = 0.1139 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:59:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:59:39: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:59:39: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:59:45: 1000000 INFO @ Thu, 08 Oct 2020 20:59:50: 2000000 INFO @ Thu, 08 Oct 2020 20:59:56: 3000000 INFO @ Thu, 08 Oct 2020 21:00:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:00:07: 5000000 INFO @ Thu, 08 Oct 2020 21:00:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:00:10: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:00:10: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:00:14: 6000000 INFO @ Thu, 08 Oct 2020 21:00:16: 1000000 INFO @ Thu, 08 Oct 2020 21:00:20: 7000000 INFO @ Thu, 08 Oct 2020 21:00:23: 2000000 INFO @ Thu, 08 Oct 2020 21:00:26: 8000000 INFO @ Thu, 08 Oct 2020 21:00:29: 3000000 INFO @ Thu, 08 Oct 2020 21:00:33: 9000000 INFO @ Thu, 08 Oct 2020 21:00:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:00:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:00:39: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:00:39: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:00:39: 10000000 INFO @ Thu, 08 Oct 2020 21:00:42: 5000000 INFO @ Thu, 08 Oct 2020 21:00:45: 11000000 INFO @ Thu, 08 Oct 2020 21:00:46: 1000000 INFO @ Thu, 08 Oct 2020 21:00:49: 6000000 INFO @ Thu, 08 Oct 2020 21:00:52: 12000000 INFO @ Thu, 08 Oct 2020 21:00:52: 2000000 INFO @ Thu, 08 Oct 2020 21:00:55: 7000000 INFO @ Thu, 08 Oct 2020 21:00:58: 13000000 INFO @ Thu, 08 Oct 2020 21:00:58: 3000000 INFO @ Thu, 08 Oct 2020 21:01:02: 8000000 INFO @ Thu, 08 Oct 2020 21:01:04: 14000000 INFO @ Thu, 08 Oct 2020 21:01:05: 4000000 INFO @ Thu, 08 Oct 2020 21:01:08: 9000000 INFO @ Thu, 08 Oct 2020 21:01:11: 15000000 INFO @ Thu, 08 Oct 2020 21:01:11: 5000000 INFO @ Thu, 08 Oct 2020 21:01:15: 10000000 INFO @ Thu, 08 Oct 2020 21:01:17: 16000000 INFO @ Thu, 08 Oct 2020 21:01:18: 6000000 INFO @ Thu, 08 Oct 2020 21:01:21: 11000000 INFO @ Thu, 08 Oct 2020 21:01:23: 17000000 INFO @ Thu, 08 Oct 2020 21:01:24: 7000000 INFO @ Thu, 08 Oct 2020 21:01:27: 12000000 INFO @ Thu, 08 Oct 2020 21:01:30: 18000000 INFO @ Thu, 08 Oct 2020 21:01:31: 8000000 INFO @ Thu, 08 Oct 2020 21:01:34: 13000000 INFO @ Thu, 08 Oct 2020 21:01:36: 19000000 INFO @ Thu, 08 Oct 2020 21:01:37: 9000000 INFO @ Thu, 08 Oct 2020 21:01:40: 14000000 INFO @ Thu, 08 Oct 2020 21:01:42: 20000000 INFO @ Thu, 08 Oct 2020 21:01:44: 10000000 INFO @ Thu, 08 Oct 2020 21:01:46: 15000000 INFO @ Thu, 08 Oct 2020 21:01:49: 21000000 INFO @ Thu, 08 Oct 2020 21:01:50: 11000000 INFO @ Thu, 08 Oct 2020 21:01:53: 16000000 INFO @ Thu, 08 Oct 2020 21:01:55: 22000000 INFO @ Thu, 08 Oct 2020 21:01:56: 12000000 INFO @ Thu, 08 Oct 2020 21:01:59: 17000000 INFO @ Thu, 08 Oct 2020 21:02:00: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:02:00: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:02:00: #1 total tags in treatment: 22773699 INFO @ Thu, 08 Oct 2020 21:02:00: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:02:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:02:00: #1 tags after filtering in treatment: 22773699 INFO @ Thu, 08 Oct 2020 21:02:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:02:00: #1 finished! INFO @ Thu, 08 Oct 2020 21:02:00: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:02:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:02:02: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 21:02:02: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:02:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:02:02: 13000000 INFO @ Thu, 08 Oct 2020 21:02:05: 18000000 INFO @ Thu, 08 Oct 2020 21:02:09: 14000000 INFO @ Thu, 08 Oct 2020 21:02:12: 19000000 INFO @ Thu, 08 Oct 2020 21:02:15: 15000000 INFO @ Thu, 08 Oct 2020 21:02:18: 20000000 INFO @ Thu, 08 Oct 2020 21:02:21: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:02:24: 21000000 INFO @ Thu, 08 Oct 2020 21:02:28: 17000000 INFO @ Thu, 08 Oct 2020 21:02:30: 22000000 INFO @ Thu, 08 Oct 2020 21:02:34: 18000000 INFO @ Thu, 08 Oct 2020 21:02:35: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:02:35: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:02:35: #1 total tags in treatment: 22773699 INFO @ Thu, 08 Oct 2020 21:02:35: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:02:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:02:35: #1 tags after filtering in treatment: 22773699 INFO @ Thu, 08 Oct 2020 21:02:35: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:02:35: #1 finished! INFO @ Thu, 08 Oct 2020 21:02:35: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:02:35: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:02:37: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 21:02:37: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:02:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:02:40: 19000000 INFO @ Thu, 08 Oct 2020 21:02:46: 20000000 INFO @ Thu, 08 Oct 2020 21:02:51: 21000000 INFO @ Thu, 08 Oct 2020 21:02:57: 22000000 INFO @ Thu, 08 Oct 2020 21:03:02: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:03:02: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:03:02: #1 total tags in treatment: 22773699 INFO @ Thu, 08 Oct 2020 21:03:02: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:03:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:03:02: #1 tags after filtering in treatment: 22773699 INFO @ Thu, 08 Oct 2020 21:03:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:03:02: #1 finished! INFO @ Thu, 08 Oct 2020 21:03:02: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:03:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:03:04: #2 number of paired peaks: 7 WARNING @ Thu, 08 Oct 2020 21:03:04: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:03:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。