Job ID = 14166985 SRX = SRX7723718 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 28473101 spots for SRR11084674/SRR11084674.sra Written 28473101 spots for SRR11084674/SRR11084674.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167334 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:36 28473101 reads; of these: 28473101 (100.00%) were unpaired; of these: 2771205 (9.73%) aligned 0 times 20322501 (71.37%) aligned exactly 1 time 5379395 (18.89%) aligned >1 times 90.27% overall alignment rate Time searching: 00:10:37 Overall time: 00:10:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2928197 / 25701896 = 0.1139 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:44:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:44:09: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:44:09: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:44:15: 1000000 INFO @ Fri, 10 Dec 2021 08:44:20: 2000000 INFO @ Fri, 10 Dec 2021 08:44:26: 3000000 INFO @ Fri, 10 Dec 2021 08:44:31: 4000000 INFO @ Fri, 10 Dec 2021 08:44:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:44:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:44:39: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:44:39: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:44:43: 6000000 INFO @ Fri, 10 Dec 2021 08:44:45: 1000000 INFO @ Fri, 10 Dec 2021 08:44:49: 7000000 INFO @ Fri, 10 Dec 2021 08:44:51: 2000000 INFO @ Fri, 10 Dec 2021 08:44:55: 8000000 INFO @ Fri, 10 Dec 2021 08:44:57: 3000000 INFO @ Fri, 10 Dec 2021 08:45:01: 9000000 INFO @ Fri, 10 Dec 2021 08:45:03: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:45:07: 10000000 INFO @ Fri, 10 Dec 2021 08:45:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:45:09: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:45:09: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:45:09: 5000000 INFO @ Fri, 10 Dec 2021 08:45:13: 11000000 INFO @ Fri, 10 Dec 2021 08:45:15: 1000000 INFO @ Fri, 10 Dec 2021 08:45:16: 6000000 INFO @ Fri, 10 Dec 2021 08:45:19: 12000000 INFO @ Fri, 10 Dec 2021 08:45:21: 2000000 INFO @ Fri, 10 Dec 2021 08:45:22: 7000000 INFO @ Fri, 10 Dec 2021 08:45:25: 13000000 INFO @ Fri, 10 Dec 2021 08:45:27: 3000000 INFO @ Fri, 10 Dec 2021 08:45:28: 8000000 INFO @ Fri, 10 Dec 2021 08:45:32: 14000000 INFO @ Fri, 10 Dec 2021 08:45:34: 4000000 INFO @ Fri, 10 Dec 2021 08:45:34: 9000000 INFO @ Fri, 10 Dec 2021 08:45:38: 15000000 INFO @ Fri, 10 Dec 2021 08:45:40: 5000000 INFO @ Fri, 10 Dec 2021 08:45:40: 10000000 INFO @ Fri, 10 Dec 2021 08:45:44: 16000000 INFO @ Fri, 10 Dec 2021 08:45:46: 6000000 INFO @ Fri, 10 Dec 2021 08:45:46: 11000000 INFO @ Fri, 10 Dec 2021 08:45:50: 17000000 INFO @ Fri, 10 Dec 2021 08:45:52: 7000000 INFO @ Fri, 10 Dec 2021 08:45:52: 12000000 INFO @ Fri, 10 Dec 2021 08:45:56: 18000000 INFO @ Fri, 10 Dec 2021 08:45:58: 8000000 INFO @ Fri, 10 Dec 2021 08:45:58: 13000000 INFO @ Fri, 10 Dec 2021 08:46:02: 19000000 INFO @ Fri, 10 Dec 2021 08:46:05: 9000000 INFO @ Fri, 10 Dec 2021 08:46:05: 14000000 INFO @ Fri, 10 Dec 2021 08:46:08: 20000000 INFO @ Fri, 10 Dec 2021 08:46:11: 10000000 INFO @ Fri, 10 Dec 2021 08:46:11: 15000000 INFO @ Fri, 10 Dec 2021 08:46:14: 21000000 INFO @ Fri, 10 Dec 2021 08:46:17: 11000000 INFO @ Fri, 10 Dec 2021 08:46:17: 16000000 INFO @ Fri, 10 Dec 2021 08:46:20: 22000000 INFO @ Fri, 10 Dec 2021 08:46:23: 12000000 INFO @ Fri, 10 Dec 2021 08:46:23: 17000000 INFO @ Fri, 10 Dec 2021 08:46:25: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:46:25: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:46:25: #1 total tags in treatment: 22773699 INFO @ Fri, 10 Dec 2021 08:46:25: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:46:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:46:26: #1 tags after filtering in treatment: 22773699 INFO @ Fri, 10 Dec 2021 08:46:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:46:26: #1 finished! INFO @ Fri, 10 Dec 2021 08:46:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:46:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:46:27: #2 number of paired peaks: 7 WARNING @ Fri, 10 Dec 2021 08:46:27: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:46:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:46:29: 13000000 INFO @ Fri, 10 Dec 2021 08:46:29: 18000000 INFO @ Fri, 10 Dec 2021 08:46:35: 14000000 INFO @ Fri, 10 Dec 2021 08:46:35: 19000000 INFO @ Fri, 10 Dec 2021 08:46:41: 15000000 INFO @ Fri, 10 Dec 2021 08:46:41: 20000000 INFO @ Fri, 10 Dec 2021 08:46:47: 21000000 INFO @ Fri, 10 Dec 2021 08:46:47: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:46:53: 22000000 INFO @ Fri, 10 Dec 2021 08:46:54: 17000000 INFO @ Fri, 10 Dec 2021 08:46:58: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:46:58: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:46:58: #1 total tags in treatment: 22773699 INFO @ Fri, 10 Dec 2021 08:46:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:46:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:46:58: #1 tags after filtering in treatment: 22773699 INFO @ Fri, 10 Dec 2021 08:46:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:46:58: #1 finished! INFO @ Fri, 10 Dec 2021 08:46:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:46:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:47:00: 18000000 INFO @ Fri, 10 Dec 2021 08:47:00: #2 number of paired peaks: 7 WARNING @ Fri, 10 Dec 2021 08:47:00: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:47:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:47:05: 19000000 INFO @ Fri, 10 Dec 2021 08:47:11: 20000000 INFO @ Fri, 10 Dec 2021 08:47:17: 21000000 INFO @ Fri, 10 Dec 2021 08:47:23: 22000000 INFO @ Fri, 10 Dec 2021 08:47:27: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:47:27: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:47:27: #1 total tags in treatment: 22773699 INFO @ Fri, 10 Dec 2021 08:47:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:47:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:47:27: #1 tags after filtering in treatment: 22773699 INFO @ Fri, 10 Dec 2021 08:47:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:47:27: #1 finished! INFO @ Fri, 10 Dec 2021 08:47:27: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:47:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:47:29: #2 number of paired peaks: 7 WARNING @ Fri, 10 Dec 2021 08:47:29: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:47:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723718/SRX7723718.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。