Job ID = 10165854 SRX = SRX7723697 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27756323 spots for SRR11084653/SRR11084653.sra Written 27756323 spots for SRR11084653/SRR11084653.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166224 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:28 27756323 reads; of these: 27756323 (100.00%) were unpaired; of these: 1863676 (6.71%) aligned 0 times 21264827 (76.61%) aligned exactly 1 time 4627820 (16.67%) aligned >1 times 93.29% overall alignment rate Time searching: 00:10:28 Overall time: 00:10:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2243277 / 25892647 = 0.0866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:20:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:20:09: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:20:09: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:20:15: 1000000 INFO @ Thu, 08 Oct 2020 20:20:21: 2000000 INFO @ Thu, 08 Oct 2020 20:20:27: 3000000 INFO @ Thu, 08 Oct 2020 20:20:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:20:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:20:38: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:20:38: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:20:39: 5000000 INFO @ Thu, 08 Oct 2020 20:20:44: 1000000 INFO @ Thu, 08 Oct 2020 20:20:45: 6000000 INFO @ Thu, 08 Oct 2020 20:20:51: 2000000 INFO @ Thu, 08 Oct 2020 20:20:52: 7000000 INFO @ Thu, 08 Oct 2020 20:20:57: 3000000 INFO @ Thu, 08 Oct 2020 20:20:58: 8000000 INFO @ Thu, 08 Oct 2020 20:21:04: 4000000 INFO @ Thu, 08 Oct 2020 20:21:04: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:21:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:21:08: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:21:08: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:21:10: 5000000 INFO @ Thu, 08 Oct 2020 20:21:11: 10000000 INFO @ Thu, 08 Oct 2020 20:21:15: 1000000 INFO @ Thu, 08 Oct 2020 20:21:17: 6000000 INFO @ Thu, 08 Oct 2020 20:21:17: 11000000 INFO @ Thu, 08 Oct 2020 20:21:22: 2000000 INFO @ Thu, 08 Oct 2020 20:21:23: 7000000 INFO @ Thu, 08 Oct 2020 20:21:24: 12000000 INFO @ Thu, 08 Oct 2020 20:21:29: 3000000 INFO @ Thu, 08 Oct 2020 20:21:29: 8000000 INFO @ Thu, 08 Oct 2020 20:21:31: 13000000 INFO @ Thu, 08 Oct 2020 20:21:36: 4000000 INFO @ Thu, 08 Oct 2020 20:21:36: 9000000 INFO @ Thu, 08 Oct 2020 20:21:37: 14000000 INFO @ Thu, 08 Oct 2020 20:21:42: 10000000 INFO @ Thu, 08 Oct 2020 20:21:43: 5000000 INFO @ Thu, 08 Oct 2020 20:21:44: 15000000 INFO @ Thu, 08 Oct 2020 20:21:49: 11000000 INFO @ Thu, 08 Oct 2020 20:21:50: 6000000 INFO @ Thu, 08 Oct 2020 20:21:51: 16000000 INFO @ Thu, 08 Oct 2020 20:21:56: 12000000 INFO @ Thu, 08 Oct 2020 20:21:57: 7000000 INFO @ Thu, 08 Oct 2020 20:21:58: 17000000 INFO @ Thu, 08 Oct 2020 20:22:03: 13000000 INFO @ Thu, 08 Oct 2020 20:22:04: 8000000 INFO @ Thu, 08 Oct 2020 20:22:04: 18000000 INFO @ Thu, 08 Oct 2020 20:22:09: 14000000 INFO @ Thu, 08 Oct 2020 20:22:11: 9000000 INFO @ Thu, 08 Oct 2020 20:22:11: 19000000 INFO @ Thu, 08 Oct 2020 20:22:15: 15000000 INFO @ Thu, 08 Oct 2020 20:22:18: 10000000 INFO @ Thu, 08 Oct 2020 20:22:18: 20000000 INFO @ Thu, 08 Oct 2020 20:22:22: 16000000 INFO @ Thu, 08 Oct 2020 20:22:25: 21000000 INFO @ Thu, 08 Oct 2020 20:22:25: 11000000 INFO @ Thu, 08 Oct 2020 20:22:29: 17000000 INFO @ Thu, 08 Oct 2020 20:22:31: 22000000 INFO @ Thu, 08 Oct 2020 20:22:32: 12000000 INFO @ Thu, 08 Oct 2020 20:22:35: 18000000 INFO @ Thu, 08 Oct 2020 20:22:38: 23000000 INFO @ Thu, 08 Oct 2020 20:22:39: 13000000 INFO @ Thu, 08 Oct 2020 20:22:42: 19000000 INFO @ Thu, 08 Oct 2020 20:22:43: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:22:43: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:22:43: #1 total tags in treatment: 23649370 INFO @ Thu, 08 Oct 2020 20:22:43: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:22:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:22:43: #1 tags after filtering in treatment: 23649370 INFO @ Thu, 08 Oct 2020 20:22:43: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:22:43: #1 finished! INFO @ Thu, 08 Oct 2020 20:22:43: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:22:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:22:45: #2 number of paired peaks: 39 WARNING @ Thu, 08 Oct 2020 20:22:45: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:22:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:22:46: 14000000 INFO @ Thu, 08 Oct 2020 20:22:49: 20000000 INFO @ Thu, 08 Oct 2020 20:22:52: 15000000 INFO @ Thu, 08 Oct 2020 20:22:56: 21000000 INFO @ Thu, 08 Oct 2020 20:22:59: 16000000 INFO @ Thu, 08 Oct 2020 20:23:02: 22000000 INFO @ Thu, 08 Oct 2020 20:23:05: 17000000 INFO @ Thu, 08 Oct 2020 20:23:09: 23000000 INFO @ Thu, 08 Oct 2020 20:23:12: 18000000 INFO @ Thu, 08 Oct 2020 20:23:13: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:23:13: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:23:13: #1 total tags in treatment: 23649370 INFO @ Thu, 08 Oct 2020 20:23:13: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:23:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:23:13: #1 tags after filtering in treatment: 23649370 INFO @ Thu, 08 Oct 2020 20:23:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:23:13: #1 finished! INFO @ Thu, 08 Oct 2020 20:23:13: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:23:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:23:15: #2 number of paired peaks: 39 WARNING @ Thu, 08 Oct 2020 20:23:15: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:23:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:23:18: 19000000 INFO @ Thu, 08 Oct 2020 20:23:24: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:23:30: 21000000 INFO @ Thu, 08 Oct 2020 20:23:36: 22000000 INFO @ Thu, 08 Oct 2020 20:23:42: 23000000 INFO @ Thu, 08 Oct 2020 20:23:47: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:23:47: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:23:47: #1 total tags in treatment: 23649370 INFO @ Thu, 08 Oct 2020 20:23:47: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:23:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:23:47: #1 tags after filtering in treatment: 23649370 INFO @ Thu, 08 Oct 2020 20:23:47: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:23:47: #1 finished! INFO @ Thu, 08 Oct 2020 20:23:47: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:23:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:23:49: #2 number of paired peaks: 39 WARNING @ Thu, 08 Oct 2020 20:23:49: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:23:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。