Job ID = 14167095 SRX = SRX7723697 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27756323 spots for SRR11084653/SRR11084653.sra Written 27756323 spots for SRR11084653/SRR11084653.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167520 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:41 27756323 reads; of these: 27756323 (100.00%) were unpaired; of these: 1863676 (6.71%) aligned 0 times 21264827 (76.61%) aligned exactly 1 time 4627820 (16.67%) aligned >1 times 93.29% overall alignment rate Time searching: 00:10:41 Overall time: 00:10:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2243277 / 25892647 = 0.0866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:06:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:06:50: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:06:50: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:06:56: 1000000 INFO @ Fri, 10 Dec 2021 10:07:02: 2000000 INFO @ Fri, 10 Dec 2021 10:07:08: 3000000 INFO @ Fri, 10 Dec 2021 10:07:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:07:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:07:19: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:07:19: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:07:20: 5000000 INFO @ Fri, 10 Dec 2021 10:07:25: 1000000 INFO @ Fri, 10 Dec 2021 10:07:26: 6000000 INFO @ Fri, 10 Dec 2021 10:07:31: 2000000 INFO @ Fri, 10 Dec 2021 10:07:33: 7000000 INFO @ Fri, 10 Dec 2021 10:07:38: 3000000 INFO @ Fri, 10 Dec 2021 10:07:39: 8000000 INFO @ Fri, 10 Dec 2021 10:07:44: 4000000 INFO @ Fri, 10 Dec 2021 10:07:46: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:07:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:07:49: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:07:49: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:07:51: 5000000 INFO @ Fri, 10 Dec 2021 10:07:53: 10000000 INFO @ Fri, 10 Dec 2021 10:07:57: 1000000 INFO @ Fri, 10 Dec 2021 10:07:59: 6000000 INFO @ Fri, 10 Dec 2021 10:08:00: 11000000 INFO @ Fri, 10 Dec 2021 10:08:04: 2000000 INFO @ Fri, 10 Dec 2021 10:08:06: 7000000 INFO @ Fri, 10 Dec 2021 10:08:08: 12000000 INFO @ Fri, 10 Dec 2021 10:08:11: 3000000 INFO @ Fri, 10 Dec 2021 10:08:13: 8000000 INFO @ Fri, 10 Dec 2021 10:08:15: 13000000 INFO @ Fri, 10 Dec 2021 10:08:18: 4000000 INFO @ Fri, 10 Dec 2021 10:08:21: 9000000 INFO @ Fri, 10 Dec 2021 10:08:23: 14000000 INFO @ Fri, 10 Dec 2021 10:08:25: 5000000 INFO @ Fri, 10 Dec 2021 10:08:28: 10000000 INFO @ Fri, 10 Dec 2021 10:08:30: 15000000 INFO @ Fri, 10 Dec 2021 10:08:32: 6000000 INFO @ Fri, 10 Dec 2021 10:08:35: 11000000 INFO @ Fri, 10 Dec 2021 10:08:37: 16000000 INFO @ Fri, 10 Dec 2021 10:08:39: 7000000 INFO @ Fri, 10 Dec 2021 10:08:43: 12000000 INFO @ Fri, 10 Dec 2021 10:08:44: 17000000 INFO @ Fri, 10 Dec 2021 10:08:46: 8000000 INFO @ Fri, 10 Dec 2021 10:08:50: 13000000 INFO @ Fri, 10 Dec 2021 10:08:52: 18000000 INFO @ Fri, 10 Dec 2021 10:08:53: 9000000 INFO @ Fri, 10 Dec 2021 10:08:57: 14000000 INFO @ Fri, 10 Dec 2021 10:08:59: 19000000 INFO @ Fri, 10 Dec 2021 10:09:00: 10000000 INFO @ Fri, 10 Dec 2021 10:09:04: 15000000 INFO @ Fri, 10 Dec 2021 10:09:06: 20000000 INFO @ Fri, 10 Dec 2021 10:09:07: 11000000 INFO @ Fri, 10 Dec 2021 10:09:11: 16000000 INFO @ Fri, 10 Dec 2021 10:09:13: 21000000 INFO @ Fri, 10 Dec 2021 10:09:14: 12000000 INFO @ Fri, 10 Dec 2021 10:09:18: 17000000 INFO @ Fri, 10 Dec 2021 10:09:20: 22000000 INFO @ Fri, 10 Dec 2021 10:09:22: 13000000 INFO @ Fri, 10 Dec 2021 10:09:25: 18000000 INFO @ Fri, 10 Dec 2021 10:09:28: 23000000 INFO @ Fri, 10 Dec 2021 10:09:29: 14000000 INFO @ Fri, 10 Dec 2021 10:09:32: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:09:32: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:09:32: #1 total tags in treatment: 23649370 INFO @ Fri, 10 Dec 2021 10:09:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:09:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:09:33: 19000000 INFO @ Fri, 10 Dec 2021 10:09:33: #1 tags after filtering in treatment: 23649370 INFO @ Fri, 10 Dec 2021 10:09:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:09:33: #1 finished! INFO @ Fri, 10 Dec 2021 10:09:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:09:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:09:35: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 10:09:35: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:09:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:09:36: 15000000 INFO @ Fri, 10 Dec 2021 10:09:40: 20000000 INFO @ Fri, 10 Dec 2021 10:09:43: 16000000 INFO @ Fri, 10 Dec 2021 10:09:46: 21000000 INFO @ Fri, 10 Dec 2021 10:09:50: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:09:53: 22000000 INFO @ Fri, 10 Dec 2021 10:09:57: 18000000 INFO @ Fri, 10 Dec 2021 10:10:00: 23000000 INFO @ Fri, 10 Dec 2021 10:10:04: 19000000 INFO @ Fri, 10 Dec 2021 10:10:05: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:10:05: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:10:05: #1 total tags in treatment: 23649370 INFO @ Fri, 10 Dec 2021 10:10:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:10:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:10:05: #1 tags after filtering in treatment: 23649370 INFO @ Fri, 10 Dec 2021 10:10:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:10:05: #1 finished! INFO @ Fri, 10 Dec 2021 10:10:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:10:07: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 10:10:07: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:10:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:10:10: 20000000 INFO @ Fri, 10 Dec 2021 10:10:17: 21000000 INFO @ Fri, 10 Dec 2021 10:10:23: 22000000 INFO @ Fri, 10 Dec 2021 10:10:29: 23000000 INFO @ Fri, 10 Dec 2021 10:10:33: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:10:33: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:10:33: #1 total tags in treatment: 23649370 INFO @ Fri, 10 Dec 2021 10:10:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:10:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:10:34: #1 tags after filtering in treatment: 23649370 INFO @ Fri, 10 Dec 2021 10:10:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:10:34: #1 finished! INFO @ Fri, 10 Dec 2021 10:10:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:10:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:10:36: #2 number of paired peaks: 39 WARNING @ Fri, 10 Dec 2021 10:10:36: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:10:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723697/SRX7723697.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。