Job ID = 6528496 SRX = SRX760498 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:10:09 prefetch.2.10.7: 1) Downloading 'SRR1653729'... 2020-06-29T15:10:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:16:14 prefetch.2.10.7: HTTPS download failed 2020-06-29T15:16:14 prefetch.2.10.7: 1) failed to download SRR1653729 2020-06-29T15:16:27 prefetch.2.10.7: 1) Downloading 'SRR1653729'... 2020-06-29T15:16:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:16:27 prefetch.2.10.7: Continue download of 'SRR1653729' from 662816986 2020-06-29T15:16:36 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:16:36 prefetch.2.10.7: 1) 'SRR1653729' was downloaded successfully 2020-06-29T15:16:36 prefetch.2.10.7: 'SRR1653729' has 0 unresolved dependencies Read 4349567 spots for SRR1653729/SRR1653729.sra Written 4349567 spots for SRR1653729/SRR1653729.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:22 4349567 reads; of these: 4349567 (100.00%) were unpaired; of these: 4349510 (100.00%) aligned 0 times 27 (0.00%) aligned exactly 1 time 30 (0.00%) aligned >1 times 0.00% overall alignment rate Time searching: 00:04:22 Overall time: 00:04:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 27 / 57 = 0.4737 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:26:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:26:34: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:26:34: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:26:34: #1 tag size is determined as 300 bps INFO @ Tue, 30 Jun 2020 00:26:34: #1 tag size = 300 INFO @ Tue, 30 Jun 2020 00:26:34: #1 total tags in treatment: 30 INFO @ Tue, 30 Jun 2020 00:26:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:26:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:26:34: #1 tags after filtering in treatment: 27 INFO @ Tue, 30 Jun 2020 00:26:34: #1 Redundant rate of treatment: 0.10 INFO @ Tue, 30 Jun 2020 00:26:34: #1 finished! INFO @ Tue, 30 Jun 2020 00:26:34: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:26:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:26:34: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:26:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:26:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:27:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:27:03: #1 tag size is determined as 300 bps INFO @ Tue, 30 Jun 2020 00:27:03: #1 tag size = 300 INFO @ Tue, 30 Jun 2020 00:27:03: #1 total tags in treatment: 30 INFO @ Tue, 30 Jun 2020 00:27:03: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:03: #1 tags after filtering in treatment: 27 INFO @ Tue, 30 Jun 2020 00:27:03: #1 Redundant rate of treatment: 0.10 INFO @ Tue, 30 Jun 2020 00:27:03: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:03: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:27:03: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:27:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:27:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:34: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:34: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:27:34: #1 tag size is determined as 300 bps INFO @ Tue, 30 Jun 2020 00:27:34: #1 tag size = 300 INFO @ Tue, 30 Jun 2020 00:27:34: #1 total tags in treatment: 30 INFO @ Tue, 30 Jun 2020 00:27:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:27:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:27:34: #1 tags after filtering in treatment: 27 INFO @ Tue, 30 Jun 2020 00:27:34: #1 Redundant rate of treatment: 0.10 INFO @ Tue, 30 Jun 2020 00:27:34: #1 finished! INFO @ Tue, 30 Jun 2020 00:27:34: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:27:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:27:34: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:27:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:27:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760498/SRX760498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling