Job ID = 12266641 SRX = SRX7572783 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 46529240 spots for SRR10904630/SRR10904630.sra Written 46529240 spots for SRR10904630/SRR10904630.sra fastq に変換しました。 bowtie でマッピング中... Your job 12271794 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:19:03 46529240 reads; of these: 46529240 (100.00%) were paired; of these: 20185894 (43.38%) aligned concordantly 0 times 20360427 (43.76%) aligned concordantly exactly 1 time 5982919 (12.86%) aligned concordantly >1 times ---- 20185894 pairs aligned concordantly 0 times; of these: 5405712 (26.78%) aligned discordantly 1 time ---- 14780182 pairs aligned 0 times concordantly or discordantly; of these: 29560364 mates make up the pairs; of these: 24713894 (83.60%) aligned 0 times 1003669 (3.40%) aligned exactly 1 time 3842801 (13.00%) aligned >1 times 73.44% overall alignment rate Time searching: 03:19:03 Overall time: 03:19:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8252696 / 31523150 = 0.2618 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 13:00:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 13:00:41: #1 read tag files... INFO @ Sat, 03 Apr 2021 13:00:41: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 13:00:50: 1000000 INFO @ Sat, 03 Apr 2021 13:00:59: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 13:01:09: 3000000 INFO @ Sat, 03 Apr 2021 13:01:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 13:01:11: #1 read tag files... INFO @ Sat, 03 Apr 2021 13:01:11: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 13:01:18: 4000000 INFO @ Sat, 03 Apr 2021 13:01:21: 1000000 INFO @ Sat, 03 Apr 2021 13:01:27: 5000000 INFO @ Sat, 03 Apr 2021 13:01:30: 2000000 INFO @ Sat, 03 Apr 2021 13:01:37: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 13:01:40: 3000000 INFO @ Sat, 03 Apr 2021 13:01:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 13:01:41: #1 read tag files... INFO @ Sat, 03 Apr 2021 13:01:41: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 13:01:46: 7000000 INFO @ Sat, 03 Apr 2021 13:01:50: 4000000 INFO @ Sat, 03 Apr 2021 13:01:51: 1000000 INFO @ Sat, 03 Apr 2021 13:01:55: 8000000 INFO @ Sat, 03 Apr 2021 13:02:01: 5000000 INFO @ Sat, 03 Apr 2021 13:02:02: 2000000 INFO @ Sat, 03 Apr 2021 13:02:05: 9000000 INFO @ Sat, 03 Apr 2021 13:02:12: 6000000 INFO @ Sat, 03 Apr 2021 13:02:13: 3000000 INFO @ Sat, 03 Apr 2021 13:02:14: 10000000 INFO @ Sat, 03 Apr 2021 13:02:22: 7000000 INFO @ Sat, 03 Apr 2021 13:02:23: 11000000 INFO @ Sat, 03 Apr 2021 13:02:24: 4000000 INFO @ Sat, 03 Apr 2021 13:02:33: 12000000 INFO @ Sat, 03 Apr 2021 13:02:33: 8000000 INFO @ Sat, 03 Apr 2021 13:02:35: 5000000 INFO @ Sat, 03 Apr 2021 13:02:42: 13000000 INFO @ Sat, 03 Apr 2021 13:02:46: 9000000 INFO @ Sat, 03 Apr 2021 13:02:47: 6000000 INFO @ Sat, 03 Apr 2021 13:02:51: 14000000 INFO @ Sat, 03 Apr 2021 13:02:56: 10000000 INFO @ Sat, 03 Apr 2021 13:02:57: 7000000 INFO @ Sat, 03 Apr 2021 13:03:00: 15000000 INFO @ Sat, 03 Apr 2021 13:03:07: 11000000 INFO @ Sat, 03 Apr 2021 13:03:08: 8000000 INFO @ Sat, 03 Apr 2021 13:03:09: 16000000 INFO @ Sat, 03 Apr 2021 13:03:18: 12000000 INFO @ Sat, 03 Apr 2021 13:03:19: 17000000 INFO @ Sat, 03 Apr 2021 13:03:19: 9000000 INFO @ Sat, 03 Apr 2021 13:03:28: 18000000 INFO @ Sat, 03 Apr 2021 13:03:29: 13000000 INFO @ Sat, 03 Apr 2021 13:03:30: 10000000 INFO @ Sat, 03 Apr 2021 13:03:37: 19000000 INFO @ Sat, 03 Apr 2021 13:03:39: 14000000 INFO @ Sat, 03 Apr 2021 13:03:40: 11000000 INFO @ Sat, 03 Apr 2021 13:03:47: 20000000 INFO @ Sat, 03 Apr 2021 13:03:51: 15000000 INFO @ Sat, 03 Apr 2021 13:03:52: 12000000 INFO @ Sat, 03 Apr 2021 13:03:56: 21000000 INFO @ Sat, 03 Apr 2021 13:04:04: 16000000 INFO @ Sat, 03 Apr 2021 13:04:05: 22000000 INFO @ Sat, 03 Apr 2021 13:04:06: 13000000 INFO @ Sat, 03 Apr 2021 13:04:15: 23000000 INFO @ Sat, 03 Apr 2021 13:04:19: 17000000 INFO @ Sat, 03 Apr 2021 13:04:20: 14000000 INFO @ Sat, 03 Apr 2021 13:04:24: 24000000 INFO @ Sat, 03 Apr 2021 13:04:32: 18000000 INFO @ Sat, 03 Apr 2021 13:04:33: 25000000 INFO @ Sat, 03 Apr 2021 13:04:34: 15000000 INFO @ Sat, 03 Apr 2021 13:04:42: 26000000 INFO @ Sat, 03 Apr 2021 13:04:46: 19000000 INFO @ Sat, 03 Apr 2021 13:04:48: 16000000 INFO @ Sat, 03 Apr 2021 13:04:51: 27000000 INFO @ Sat, 03 Apr 2021 13:05:00: 20000000 INFO @ Sat, 03 Apr 2021 13:05:01: 28000000 INFO @ Sat, 03 Apr 2021 13:05:01: 17000000 INFO @ Sat, 03 Apr 2021 13:05:10: 29000000 INFO @ Sat, 03 Apr 2021 13:05:14: 21000000 INFO @ Sat, 03 Apr 2021 13:05:15: 18000000 INFO @ Sat, 03 Apr 2021 13:05:20: 30000000 INFO @ Sat, 03 Apr 2021 13:05:27: 22000000 INFO @ Sat, 03 Apr 2021 13:05:28: 19000000 INFO @ Sat, 03 Apr 2021 13:05:29: 31000000 INFO @ Sat, 03 Apr 2021 13:05:38: 32000000 INFO @ Sat, 03 Apr 2021 13:05:41: 23000000 INFO @ Sat, 03 Apr 2021 13:05:42: 20000000 INFO @ Sat, 03 Apr 2021 13:05:48: 33000000 INFO @ Sat, 03 Apr 2021 13:05:56: 24000000 INFO @ Sat, 03 Apr 2021 13:05:57: 21000000 INFO @ Sat, 03 Apr 2021 13:05:57: 34000000 INFO @ Sat, 03 Apr 2021 13:06:07: 35000000 INFO @ Sat, 03 Apr 2021 13:06:09: 25000000 INFO @ Sat, 03 Apr 2021 13:06:11: 22000000 INFO @ Sat, 03 Apr 2021 13:06:16: 36000000 INFO @ Sat, 03 Apr 2021 13:06:23: 26000000 INFO @ Sat, 03 Apr 2021 13:06:25: 23000000 INFO @ Sat, 03 Apr 2021 13:06:25: 37000000 INFO @ Sat, 03 Apr 2021 13:06:35: 38000000 INFO @ Sat, 03 Apr 2021 13:06:37: 27000000 INFO @ Sat, 03 Apr 2021 13:06:38: 24000000 INFO @ Sat, 03 Apr 2021 13:06:44: 39000000 INFO @ Sat, 03 Apr 2021 13:06:51: 28000000 INFO @ Sat, 03 Apr 2021 13:06:52: 25000000 INFO @ Sat, 03 Apr 2021 13:06:53: 40000000 INFO @ Sat, 03 Apr 2021 13:07:03: 41000000 INFO @ Sat, 03 Apr 2021 13:07:04: 29000000 INFO @ Sat, 03 Apr 2021 13:07:05: 26000000 INFO @ Sat, 03 Apr 2021 13:07:12: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 13:07:18: 30000000 INFO @ Sat, 03 Apr 2021 13:07:19: 27000000 INFO @ Sat, 03 Apr 2021 13:07:21: 43000000 INFO @ Sat, 03 Apr 2021 13:07:31: 44000000 INFO @ Sat, 03 Apr 2021 13:07:33: 31000000 INFO @ Sat, 03 Apr 2021 13:07:34: 28000000 INFO @ Sat, 03 Apr 2021 13:07:41: 45000000 INFO @ Sat, 03 Apr 2021 13:07:46: 32000000 INFO @ Sat, 03 Apr 2021 13:07:47: 29000000 INFO @ Sat, 03 Apr 2021 13:07:50: 46000000 INFO @ Sat, 03 Apr 2021 13:07:59: 47000000 INFO @ Sat, 03 Apr 2021 13:08:00: 33000000 INFO @ Sat, 03 Apr 2021 13:08:01: 30000000 INFO @ Sat, 03 Apr 2021 13:08:08: 48000000 INFO @ Sat, 03 Apr 2021 13:08:14: 34000000 INFO @ Sat, 03 Apr 2021 13:08:14: 31000000 INFO @ Sat, 03 Apr 2021 13:08:18: 49000000 INFO @ Sat, 03 Apr 2021 13:08:27: 35000000 INFO @ Sat, 03 Apr 2021 13:08:27: 50000000 INFO @ Sat, 03 Apr 2021 13:08:27: 32000000 INFO @ Sat, 03 Apr 2021 13:08:36: 51000000 INFO @ Sat, 03 Apr 2021 13:08:40: 36000000 INFO @ Sat, 03 Apr 2021 13:08:40: 33000000 INFO @ Sat, 03 Apr 2021 13:08:44: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 13:08:44: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 13:08:44: #1 total tags in treatment: 19268039 INFO @ Sat, 03 Apr 2021 13:08:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 13:08:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 13:08:44: #1 tags after filtering in treatment: 15720305 INFO @ Sat, 03 Apr 2021 13:08:44: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 13:08:44: #1 finished! INFO @ Sat, 03 Apr 2021 13:08:44: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 13:08:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 13:08:45: #2 number of paired peaks: 84 WARNING @ Sat, 03 Apr 2021 13:08:45: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 13:08:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 13:08:53: 37000000 INFO @ Sat, 03 Apr 2021 13:08:53: 34000000 INFO @ Sat, 03 Apr 2021 13:09:07: 38000000 INFO @ Sat, 03 Apr 2021 13:09:08: 35000000 INFO @ Sat, 03 Apr 2021 13:09:20: 39000000 INFO @ Sat, 03 Apr 2021 13:09:20: 36000000 INFO @ Sat, 03 Apr 2021 13:09:33: 40000000 INFO @ Sat, 03 Apr 2021 13:09:33: 37000000 INFO @ Sat, 03 Apr 2021 13:09:46: 38000000 INFO @ Sat, 03 Apr 2021 13:09:46: 41000000 INFO @ Sat, 03 Apr 2021 13:09:58: 39000000 INFO @ Sat, 03 Apr 2021 13:09:58: 42000000 INFO @ Sat, 03 Apr 2021 13:10:09: 40000000 INFO @ Sat, 03 Apr 2021 13:10:10: 43000000 INFO @ Sat, 03 Apr 2021 13:10:20: 41000000 INFO @ Sat, 03 Apr 2021 13:10:21: 44000000 INFO @ Sat, 03 Apr 2021 13:10:31: 42000000 INFO @ Sat, 03 Apr 2021 13:10:33: 45000000 INFO @ Sat, 03 Apr 2021 13:10:43: 43000000 INFO @ Sat, 03 Apr 2021 13:10:45: 46000000 INFO @ Sat, 03 Apr 2021 13:10:54: 44000000 INFO @ Sat, 03 Apr 2021 13:10:56: 47000000 INFO @ Sat, 03 Apr 2021 13:11:05: 45000000 INFO @ Sat, 03 Apr 2021 13:11:06: 48000000 INFO @ Sat, 03 Apr 2021 13:11:16: 46000000 INFO @ Sat, 03 Apr 2021 13:11:17: 49000000 INFO @ Sat, 03 Apr 2021 13:11:26: 47000000 INFO @ Sat, 03 Apr 2021 13:11:27: 50000000 INFO @ Sat, 03 Apr 2021 13:11:36: 48000000 INFO @ Sat, 03 Apr 2021 13:11:38: 51000000 INFO @ Sat, 03 Apr 2021 13:11:46: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 13:11:46: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 13:11:46: #1 total tags in treatment: 19268039 INFO @ Sat, 03 Apr 2021 13:11:46: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 13:11:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 13:11:46: 49000000 INFO @ Sat, 03 Apr 2021 13:11:47: #1 tags after filtering in treatment: 15720305 INFO @ Sat, 03 Apr 2021 13:11:47: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 13:11:47: #1 finished! INFO @ Sat, 03 Apr 2021 13:11:47: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 13:11:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 13:11:48: #2 number of paired peaks: 84 WARNING @ Sat, 03 Apr 2021 13:11:48: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 13:11:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 13:11:55: 50000000 INFO @ Sat, 03 Apr 2021 13:12:04: 51000000 INFO @ Sat, 03 Apr 2021 13:12:12: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 13:12:12: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 13:12:12: #1 total tags in treatment: 19268039 INFO @ Sat, 03 Apr 2021 13:12:12: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 13:12:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 13:12:13: #1 tags after filtering in treatment: 15720305 INFO @ Sat, 03 Apr 2021 13:12:13: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 13:12:13: #1 finished! INFO @ Sat, 03 Apr 2021 13:12:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 13:12:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 13:12:14: #2 number of paired peaks: 84 WARNING @ Sat, 03 Apr 2021 13:12:14: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 13:12:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572783/SRX7572783.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling