Job ID = 6626766 SRX = SRX7569438 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 33754862 spots for SRR10901260/SRR10901260.sra Written 33754862 spots for SRR10901260/SRR10901260.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627038 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:46 33754862 reads; of these: 33754862 (100.00%) were unpaired; of these: 2810967 (8.33%) aligned 0 times 27132030 (80.38%) aligned exactly 1 time 3811865 (11.29%) aligned >1 times 91.67% overall alignment rate Time searching: 00:07:46 Overall time: 00:07:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8467477 / 30943895 = 0.2736 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:54:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:54:58: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:54:58: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:55:04: 1000000 INFO @ Tue, 14 Jul 2020 08:55:10: 2000000 INFO @ Tue, 14 Jul 2020 08:55:15: 3000000 INFO @ Tue, 14 Jul 2020 08:55:21: 4000000 INFO @ Tue, 14 Jul 2020 08:55:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:55:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:55:28: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:55:28: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:55:32: 6000000 INFO @ Tue, 14 Jul 2020 08:55:35: 1000000 INFO @ Tue, 14 Jul 2020 08:55:38: 7000000 INFO @ Tue, 14 Jul 2020 08:55:42: 2000000 INFO @ Tue, 14 Jul 2020 08:55:44: 8000000 INFO @ Tue, 14 Jul 2020 08:55:48: 3000000 INFO @ Tue, 14 Jul 2020 08:55:50: 9000000 INFO @ Tue, 14 Jul 2020 08:55:55: 4000000 INFO @ Tue, 14 Jul 2020 08:55:56: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:55:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:55:58: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:55:58: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:56:02: 5000000 INFO @ Tue, 14 Jul 2020 08:56:03: 11000000 INFO @ Tue, 14 Jul 2020 08:56:05: 1000000 INFO @ Tue, 14 Jul 2020 08:56:09: 12000000 INFO @ Tue, 14 Jul 2020 08:56:09: 6000000 INFO @ Tue, 14 Jul 2020 08:56:11: 2000000 INFO @ Tue, 14 Jul 2020 08:56:15: 13000000 INFO @ Tue, 14 Jul 2020 08:56:15: 7000000 INFO @ Tue, 14 Jul 2020 08:56:17: 3000000 INFO @ Tue, 14 Jul 2020 08:56:21: 14000000 INFO @ Tue, 14 Jul 2020 08:56:22: 8000000 INFO @ Tue, 14 Jul 2020 08:56:23: 4000000 INFO @ Tue, 14 Jul 2020 08:56:27: 15000000 INFO @ Tue, 14 Jul 2020 08:56:29: 9000000 INFO @ Tue, 14 Jul 2020 08:56:29: 5000000 INFO @ Tue, 14 Jul 2020 08:56:33: 16000000 INFO @ Tue, 14 Jul 2020 08:56:35: 10000000 INFO @ Tue, 14 Jul 2020 08:56:36: 6000000 INFO @ Tue, 14 Jul 2020 08:56:40: 17000000 INFO @ Tue, 14 Jul 2020 08:56:42: 11000000 INFO @ Tue, 14 Jul 2020 08:56:42: 7000000 INFO @ Tue, 14 Jul 2020 08:56:46: 18000000 INFO @ Tue, 14 Jul 2020 08:56:48: 8000000 INFO @ Tue, 14 Jul 2020 08:56:48: 12000000 INFO @ Tue, 14 Jul 2020 08:56:52: 19000000 INFO @ Tue, 14 Jul 2020 08:56:54: 9000000 INFO @ Tue, 14 Jul 2020 08:56:55: 13000000 INFO @ Tue, 14 Jul 2020 08:56:58: 20000000 INFO @ Tue, 14 Jul 2020 08:57:01: 10000000 INFO @ Tue, 14 Jul 2020 08:57:02: 14000000 INFO @ Tue, 14 Jul 2020 08:57:05: 21000000 INFO @ Tue, 14 Jul 2020 08:57:07: 11000000 INFO @ Tue, 14 Jul 2020 08:57:08: 15000000 INFO @ Tue, 14 Jul 2020 08:57:11: 22000000 INFO @ Tue, 14 Jul 2020 08:57:13: 12000000 INFO @ Tue, 14 Jul 2020 08:57:14: #1 tag size is determined as 49 bps INFO @ Tue, 14 Jul 2020 08:57:14: #1 tag size = 49 INFO @ Tue, 14 Jul 2020 08:57:14: #1 total tags in treatment: 22476418 INFO @ Tue, 14 Jul 2020 08:57:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:14: #1 tags after filtering in treatment: 22476418 INFO @ Tue, 14 Jul 2020 08:57:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:57:14: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:57:15: 16000000 INFO @ Tue, 14 Jul 2020 08:57:16: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:57:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:57:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:57:19: 13000000 INFO @ Tue, 14 Jul 2020 08:57:21: 17000000 INFO @ Tue, 14 Jul 2020 08:57:25: 14000000 INFO @ Tue, 14 Jul 2020 08:57:28: 18000000 INFO @ Tue, 14 Jul 2020 08:57:31: 15000000 INFO @ Tue, 14 Jul 2020 08:57:34: 19000000 INFO @ Tue, 14 Jul 2020 08:57:37: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:57:41: 20000000 INFO @ Tue, 14 Jul 2020 08:57:44: 17000000 INFO @ Tue, 14 Jul 2020 08:57:47: 21000000 INFO @ Tue, 14 Jul 2020 08:57:50: 18000000 INFO @ Tue, 14 Jul 2020 08:57:54: 22000000 INFO @ Tue, 14 Jul 2020 08:57:56: 19000000 INFO @ Tue, 14 Jul 2020 08:57:57: #1 tag size is determined as 49 bps INFO @ Tue, 14 Jul 2020 08:57:57: #1 tag size = 49 INFO @ Tue, 14 Jul 2020 08:57:57: #1 total tags in treatment: 22476418 INFO @ Tue, 14 Jul 2020 08:57:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:58: #1 tags after filtering in treatment: 22476418 INFO @ Tue, 14 Jul 2020 08:57:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:57:58: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:58: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:57:59: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:57:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:57:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:58:02: 20000000 INFO @ Tue, 14 Jul 2020 08:58:07: 21000000 INFO @ Tue, 14 Jul 2020 08:58:13: 22000000 INFO @ Tue, 14 Jul 2020 08:58:16: #1 tag size is determined as 49 bps INFO @ Tue, 14 Jul 2020 08:58:16: #1 tag size = 49 INFO @ Tue, 14 Jul 2020 08:58:16: #1 total tags in treatment: 22476418 INFO @ Tue, 14 Jul 2020 08:58:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:58:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:58:16: #1 tags after filtering in treatment: 22476418 INFO @ Tue, 14 Jul 2020 08:58:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:58:16: #1 finished! INFO @ Tue, 14 Jul 2020 08:58:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:58:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:58:18: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:58:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:58:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569438/SRX7569438.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。