Job ID = 6626764 SRX = SRX7569437 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19681372 spots for SRR10901259/SRR10901259.sra Written 19681372 spots for SRR10901259/SRR10901259.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627020 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:42 19681372 reads; of these: 19681372 (100.00%) were unpaired; of these: 3024705 (15.37%) aligned 0 times 14815229 (75.28%) aligned exactly 1 time 1841438 (9.36%) aligned >1 times 84.63% overall alignment rate Time searching: 00:04:42 Overall time: 00:04:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3161289 / 16656667 = 0.1898 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:47:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:47:41: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:47:41: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:47:46: 1000000 INFO @ Tue, 14 Jul 2020 08:47:51: 2000000 INFO @ Tue, 14 Jul 2020 08:47:56: 3000000 INFO @ Tue, 14 Jul 2020 08:48:01: 4000000 INFO @ Tue, 14 Jul 2020 08:48:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:48:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:48:11: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:48:11: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:48:12: 6000000 INFO @ Tue, 14 Jul 2020 08:48:17: 1000000 INFO @ Tue, 14 Jul 2020 08:48:17: 7000000 INFO @ Tue, 14 Jul 2020 08:48:22: 8000000 INFO @ Tue, 14 Jul 2020 08:48:23: 2000000 INFO @ Tue, 14 Jul 2020 08:48:28: 9000000 INFO @ Tue, 14 Jul 2020 08:48:29: 3000000 INFO @ Tue, 14 Jul 2020 08:48:33: 10000000 INFO @ Tue, 14 Jul 2020 08:48:35: 4000000 INFO @ Tue, 14 Jul 2020 08:48:38: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:48:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:48:41: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:48:41: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:48:41: 5000000 INFO @ Tue, 14 Jul 2020 08:48:44: 12000000 INFO @ Tue, 14 Jul 2020 08:48:46: 1000000 INFO @ Tue, 14 Jul 2020 08:48:47: 6000000 INFO @ Tue, 14 Jul 2020 08:48:49: 13000000 INFO @ Tue, 14 Jul 2020 08:48:52: 2000000 INFO @ Tue, 14 Jul 2020 08:48:52: #1 tag size is determined as 49 bps INFO @ Tue, 14 Jul 2020 08:48:52: #1 tag size = 49 INFO @ Tue, 14 Jul 2020 08:48:52: #1 total tags in treatment: 13495378 INFO @ Tue, 14 Jul 2020 08:48:52: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:48:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:48:53: #1 tags after filtering in treatment: 13495378 INFO @ Tue, 14 Jul 2020 08:48:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:48:53: #1 finished! INFO @ Tue, 14 Jul 2020 08:48:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:48:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:48:53: 7000000 INFO @ Tue, 14 Jul 2020 08:48:53: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 08:48:53: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:48:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:48:57: 3000000 INFO @ Tue, 14 Jul 2020 08:48:59: 8000000 INFO @ Tue, 14 Jul 2020 08:49:03: 4000000 INFO @ Tue, 14 Jul 2020 08:49:05: 9000000 INFO @ Tue, 14 Jul 2020 08:49:08: 5000000 INFO @ Tue, 14 Jul 2020 08:49:11: 10000000 INFO @ Tue, 14 Jul 2020 08:49:13: 6000000 INFO @ Tue, 14 Jul 2020 08:49:17: 11000000 INFO @ Tue, 14 Jul 2020 08:49:19: 7000000 INFO @ Tue, 14 Jul 2020 08:49:23: 12000000 INFO @ Tue, 14 Jul 2020 08:49:24: 8000000 INFO @ Tue, 14 Jul 2020 08:49:29: 13000000 INFO @ Tue, 14 Jul 2020 08:49:30: 9000000 INFO @ Tue, 14 Jul 2020 08:49:32: #1 tag size is determined as 49 bps INFO @ Tue, 14 Jul 2020 08:49:32: #1 tag size = 49 INFO @ Tue, 14 Jul 2020 08:49:32: #1 total tags in treatment: 13495378 INFO @ Tue, 14 Jul 2020 08:49:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:49:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:49:32: #1 tags after filtering in treatment: 13495378 INFO @ Tue, 14 Jul 2020 08:49:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:49:32: #1 finished! INFO @ Tue, 14 Jul 2020 08:49:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:49:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:49:33: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 08:49:33: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:49:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:49:35: 10000000 INFO @ Tue, 14 Jul 2020 08:49:40: 11000000 INFO @ Tue, 14 Jul 2020 08:49:45: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:49:51: 13000000 INFO @ Tue, 14 Jul 2020 08:49:53: #1 tag size is determined as 49 bps INFO @ Tue, 14 Jul 2020 08:49:53: #1 tag size = 49 INFO @ Tue, 14 Jul 2020 08:49:53: #1 total tags in treatment: 13495378 INFO @ Tue, 14 Jul 2020 08:49:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:49:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:49:53: #1 tags after filtering in treatment: 13495378 INFO @ Tue, 14 Jul 2020 08:49:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:49:53: #1 finished! INFO @ Tue, 14 Jul 2020 08:49:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:49:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:49:54: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 08:49:54: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:49:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569437/SRX7569437.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。