Job ID = 14166955 SRX = SRX7541120 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25954152 spots for SRR10871150/SRR10871150.sra Written 25954152 spots for SRR10871150/SRR10871150.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167277 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:38 25954152 reads; of these: 25954152 (100.00%) were unpaired; of these: 5302952 (20.43%) aligned 0 times 17513207 (67.48%) aligned exactly 1 time 3137993 (12.09%) aligned >1 times 79.57% overall alignment rate Time searching: 00:07:38 Overall time: 00:07:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4416679 / 20651200 = 0.2139 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:20:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:20:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:20:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:21:08: 1000000 INFO @ Fri, 10 Dec 2021 08:21:16: 2000000 INFO @ Fri, 10 Dec 2021 08:21:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:21:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:21:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:21:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:21:32: 4000000 INFO @ Fri, 10 Dec 2021 08:21:38: 1000000 INFO @ Fri, 10 Dec 2021 08:21:41: 5000000 INFO @ Fri, 10 Dec 2021 08:21:46: 2000000 INFO @ Fri, 10 Dec 2021 08:21:50: 6000000 INFO @ Fri, 10 Dec 2021 08:21:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:21:58: 7000000 INFO @ Fri, 10 Dec 2021 08:21:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:21:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:21:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:22:03: 4000000 INFO @ Fri, 10 Dec 2021 08:22:07: 8000000 INFO @ Fri, 10 Dec 2021 08:22:08: 1000000 INFO @ Fri, 10 Dec 2021 08:22:12: 5000000 INFO @ Fri, 10 Dec 2021 08:22:16: 9000000 INFO @ Fri, 10 Dec 2021 08:22:17: 2000000 INFO @ Fri, 10 Dec 2021 08:22:20: 6000000 INFO @ Fri, 10 Dec 2021 08:22:24: 10000000 INFO @ Fri, 10 Dec 2021 08:22:25: 3000000 INFO @ Fri, 10 Dec 2021 08:22:29: 7000000 INFO @ Fri, 10 Dec 2021 08:22:33: 11000000 INFO @ Fri, 10 Dec 2021 08:22:34: 4000000 INFO @ Fri, 10 Dec 2021 08:22:37: 8000000 INFO @ Fri, 10 Dec 2021 08:22:41: 12000000 INFO @ Fri, 10 Dec 2021 08:22:43: 5000000 INFO @ Fri, 10 Dec 2021 08:22:46: 9000000 INFO @ Fri, 10 Dec 2021 08:22:49: 13000000 INFO @ Fri, 10 Dec 2021 08:22:51: 6000000 INFO @ Fri, 10 Dec 2021 08:22:54: 10000000 INFO @ Fri, 10 Dec 2021 08:22:57: 14000000 INFO @ Fri, 10 Dec 2021 08:23:00: 7000000 INFO @ Fri, 10 Dec 2021 08:23:02: 11000000 INFO @ Fri, 10 Dec 2021 08:23:05: 15000000 INFO @ Fri, 10 Dec 2021 08:23:09: 8000000 INFO @ Fri, 10 Dec 2021 08:23:11: 12000000 INFO @ Fri, 10 Dec 2021 08:23:14: 16000000 INFO @ Fri, 10 Dec 2021 08:23:16: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 08:23:16: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 08:23:16: #1 total tags in treatment: 16234521 INFO @ Fri, 10 Dec 2021 08:23:16: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:23:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:23:16: #1 tags after filtering in treatment: 16234521 INFO @ Fri, 10 Dec 2021 08:23:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:23:16: #1 finished! INFO @ Fri, 10 Dec 2021 08:23:16: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:23:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:23:18: 9000000 INFO @ Fri, 10 Dec 2021 08:23:18: #2 number of paired peaks: 6295 INFO @ Fri, 10 Dec 2021 08:23:18: start model_add_line... INFO @ Fri, 10 Dec 2021 08:23:19: start X-correlation... INFO @ Fri, 10 Dec 2021 08:23:19: end of X-cor INFO @ Fri, 10 Dec 2021 08:23:19: #2 finished! INFO @ Fri, 10 Dec 2021 08:23:19: #2 predicted fragment length is 280 bps INFO @ Fri, 10 Dec 2021 08:23:19: #2 alternative fragment length(s) may be 280 bps INFO @ Fri, 10 Dec 2021 08:23:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.05_model.r INFO @ Fri, 10 Dec 2021 08:23:19: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:23:19: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:23:20: 13000000 INFO @ Fri, 10 Dec 2021 08:23:26: 10000000 INFO @ Fri, 10 Dec 2021 08:23:29: 14000000 INFO @ Fri, 10 Dec 2021 08:23:35: 11000000 INFO @ Fri, 10 Dec 2021 08:23:37: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:23:43: 12000000 INFO @ Fri, 10 Dec 2021 08:23:45: 16000000 INFO @ Fri, 10 Dec 2021 08:23:47: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 08:23:47: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 08:23:47: #1 total tags in treatment: 16234521 INFO @ Fri, 10 Dec 2021 08:23:47: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:23:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:23:48: #1 tags after filtering in treatment: 16234521 INFO @ Fri, 10 Dec 2021 08:23:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:23:48: #1 finished! INFO @ Fri, 10 Dec 2021 08:23:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:23:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:23:50: #2 number of paired peaks: 6295 INFO @ Fri, 10 Dec 2021 08:23:50: start model_add_line... INFO @ Fri, 10 Dec 2021 08:23:50: start X-correlation... INFO @ Fri, 10 Dec 2021 08:23:50: end of X-cor INFO @ Fri, 10 Dec 2021 08:23:50: #2 finished! INFO @ Fri, 10 Dec 2021 08:23:50: #2 predicted fragment length is 280 bps INFO @ Fri, 10 Dec 2021 08:23:50: #2 alternative fragment length(s) may be 280 bps INFO @ Fri, 10 Dec 2021 08:23:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.10_model.r INFO @ Fri, 10 Dec 2021 08:23:50: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:23:50: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:23:51: 13000000 INFO @ Fri, 10 Dec 2021 08:23:59: 14000000 INFO @ Fri, 10 Dec 2021 08:24:07: 15000000 INFO @ Fri, 10 Dec 2021 08:24:15: 16000000 INFO @ Fri, 10 Dec 2021 08:24:16: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:24:17: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 08:24:17: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 08:24:17: #1 total tags in treatment: 16234521 INFO @ Fri, 10 Dec 2021 08:24:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:24:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:24:18: #1 tags after filtering in treatment: 16234521 INFO @ Fri, 10 Dec 2021 08:24:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:24:18: #1 finished! INFO @ Fri, 10 Dec 2021 08:24:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:24:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:24:20: #2 number of paired peaks: 6295 INFO @ Fri, 10 Dec 2021 08:24:20: start model_add_line... INFO @ Fri, 10 Dec 2021 08:24:20: start X-correlation... INFO @ Fri, 10 Dec 2021 08:24:20: end of X-cor INFO @ Fri, 10 Dec 2021 08:24:20: #2 finished! INFO @ Fri, 10 Dec 2021 08:24:20: #2 predicted fragment length is 280 bps INFO @ Fri, 10 Dec 2021 08:24:20: #2 alternative fragment length(s) may be 280 bps INFO @ Fri, 10 Dec 2021 08:24:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.20_model.r INFO @ Fri, 10 Dec 2021 08:24:20: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:24:20: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:24:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.05_peaks.xls INFO @ Fri, 10 Dec 2021 08:24:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:24:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.05_summits.bed INFO @ Fri, 10 Dec 2021 08:24:38: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7059 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:24:47: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:25:10: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.10_peaks.xls INFO @ Fri, 10 Dec 2021 08:25:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:25:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.10_summits.bed INFO @ Fri, 10 Dec 2021 08:25:10: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6293 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:25:18: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:25:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.20_peaks.xls INFO @ Fri, 10 Dec 2021 08:25:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:25:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7541120/SRX7541120.20_summits.bed INFO @ Fri, 10 Dec 2021 08:25:40: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (5419 records, 4 fields): 16 millis CompletedMACS2peakCalling