Job ID = 1309036 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 46,599,221 reads read : 46,599,221 reads written : 46,599,221 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:48 46599221 reads; of these: 46599221 (100.00%) were unpaired; of these: 1163587 (2.50%) aligned 0 times 17579433 (37.72%) aligned exactly 1 time 27856201 (59.78%) aligned >1 times 97.50% overall alignment rate Time searching: 00:31:48 Overall time: 00:31:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9671437 / 45435634 = 0.2129 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 04 Jun 2019 00:11:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Jun 2019 00:11:13: #1 read tag files... INFO @ Tue, 04 Jun 2019 00:11:13: #1 read treatment tags... INFO @ Tue, 04 Jun 2019 00:11:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Jun 2019 00:11:13: #1 read tag files... INFO @ Tue, 04 Jun 2019 00:11:13: #1 read treatment tags... INFO @ Tue, 04 Jun 2019 00:11:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Jun 2019 00:11:13: #1 read tag files... INFO @ Tue, 04 Jun 2019 00:11:13: #1 read treatment tags... INFO @ Tue, 04 Jun 2019 00:11:21: 1000000 INFO @ Tue, 04 Jun 2019 00:11:21: 1000000 INFO @ Tue, 04 Jun 2019 00:11:22: 1000000 INFO @ Tue, 04 Jun 2019 00:11:28: 2000000 INFO @ Tue, 04 Jun 2019 00:11:28: 2000000 INFO @ Tue, 04 Jun 2019 00:11:30: 2000000 INFO @ Tue, 04 Jun 2019 00:11:35: 3000000 INFO @ Tue, 04 Jun 2019 00:11:35: 3000000 INFO @ Tue, 04 Jun 2019 00:11:38: 3000000 INFO @ Tue, 04 Jun 2019 00:11:42: 4000000 INFO @ Tue, 04 Jun 2019 00:11:43: 4000000 INFO @ Tue, 04 Jun 2019 00:11:46: 4000000 INFO @ Tue, 04 Jun 2019 00:11:49: 5000000 INFO @ Tue, 04 Jun 2019 00:11:50: 5000000 INFO @ Tue, 04 Jun 2019 00:11:54: 5000000 INFO @ Tue, 04 Jun 2019 00:11:56: 6000000 INFO @ Tue, 04 Jun 2019 00:11:57: 6000000 INFO @ Tue, 04 Jun 2019 00:12:02: 6000000 INFO @ Tue, 04 Jun 2019 00:12:03: 7000000 INFO @ Tue, 04 Jun 2019 00:12:04: 7000000 INFO @ Tue, 04 Jun 2019 00:12:10: 7000000 INFO @ Tue, 04 Jun 2019 00:12:10: 8000000 INFO @ Tue, 04 Jun 2019 00:12:11: 8000000 INFO @ Tue, 04 Jun 2019 00:12:18: 9000000 INFO @ Tue, 04 Jun 2019 00:12:18: 9000000 INFO @ Tue, 04 Jun 2019 00:12:19: 8000000 INFO @ Tue, 04 Jun 2019 00:12:24: 10000000 INFO @ Tue, 04 Jun 2019 00:12:25: 10000000 INFO @ Tue, 04 Jun 2019 00:12:27: 9000000 INFO @ Tue, 04 Jun 2019 00:12:31: 11000000 INFO @ Tue, 04 Jun 2019 00:12:32: 11000000 INFO @ Tue, 04 Jun 2019 00:12:35: 10000000 INFO @ Tue, 04 Jun 2019 00:12:38: 12000000 INFO @ Tue, 04 Jun 2019 00:12:39: 12000000 INFO @ Tue, 04 Jun 2019 00:12:43: 11000000 INFO @ Tue, 04 Jun 2019 00:12:45: 13000000 INFO @ Tue, 04 Jun 2019 00:12:46: 13000000 INFO @ Tue, 04 Jun 2019 00:12:51: 12000000 INFO @ Tue, 04 Jun 2019 00:12:52: 14000000 INFO @ Tue, 04 Jun 2019 00:12:53: 14000000 INFO @ Tue, 04 Jun 2019 00:12:59: 13000000 INFO @ Tue, 04 Jun 2019 00:12:59: 15000000 INFO @ Tue, 04 Jun 2019 00:13:00: 15000000 INFO @ Tue, 04 Jun 2019 00:13:06: 16000000 INFO @ Tue, 04 Jun 2019 00:13:07: 16000000 INFO @ Tue, 04 Jun 2019 00:13:07: 14000000 INFO @ Tue, 04 Jun 2019 00:13:13: 17000000 INFO @ Tue, 04 Jun 2019 00:13:14: 17000000 INFO @ Tue, 04 Jun 2019 00:13:15: 15000000 INFO @ Tue, 04 Jun 2019 00:13:20: 18000000 INFO @ Tue, 04 Jun 2019 00:13:21: 18000000 INFO @ Tue, 04 Jun 2019 00:13:23: 16000000 INFO @ Tue, 04 Jun 2019 00:13:28: 19000000 INFO @ Tue, 04 Jun 2019 00:13:28: 19000000 INFO @ Tue, 04 Jun 2019 00:13:31: 17000000 INFO @ Tue, 04 Jun 2019 00:13:36: 20000000 INFO @ Tue, 04 Jun 2019 00:13:36: 20000000 INFO @ Tue, 04 Jun 2019 00:13:38: 18000000 INFO @ Tue, 04 Jun 2019 00:13:43: 21000000 INFO @ Tue, 04 Jun 2019 00:13:44: 21000000 INFO @ Tue, 04 Jun 2019 00:13:47: 19000000 INFO @ Tue, 04 Jun 2019 00:13:51: 22000000 INFO @ Tue, 04 Jun 2019 00:13:51: 22000000 INFO @ Tue, 04 Jun 2019 00:13:56: 20000000 INFO @ Tue, 04 Jun 2019 00:13:59: 23000000 INFO @ Tue, 04 Jun 2019 00:13:59: 23000000 INFO @ Tue, 04 Jun 2019 00:14:05: 21000000 INFO @ Tue, 04 Jun 2019 00:14:07: 24000000 INFO @ Tue, 04 Jun 2019 00:14:07: 24000000 INFO @ Tue, 04 Jun 2019 00:14:14: 22000000 INFO @ Tue, 04 Jun 2019 00:14:15: 25000000 INFO @ Tue, 04 Jun 2019 00:14:15: 25000000 INFO @ Tue, 04 Jun 2019 00:14:23: 26000000 INFO @ Tue, 04 Jun 2019 00:14:23: 26000000 INFO @ Tue, 04 Jun 2019 00:14:24: 23000000 INFO @ Tue, 04 Jun 2019 00:14:30: 27000000 INFO @ Tue, 04 Jun 2019 00:14:31: 27000000 INFO @ Tue, 04 Jun 2019 00:14:33: 24000000 INFO @ Tue, 04 Jun 2019 00:14:38: 28000000 INFO @ Tue, 04 Jun 2019 00:14:39: 28000000 INFO @ Tue, 04 Jun 2019 00:14:42: 25000000 INFO @ Tue, 04 Jun 2019 00:14:46: 29000000 INFO @ Tue, 04 Jun 2019 00:14:46: 29000000 INFO @ Tue, 04 Jun 2019 00:14:52: 26000000 INFO @ Tue, 04 Jun 2019 00:14:54: 30000000 INFO @ Tue, 04 Jun 2019 00:14:54: 30000000 INFO @ Tue, 04 Jun 2019 00:15:00: 27000000 INFO @ Tue, 04 Jun 2019 00:15:02: 31000000 INFO @ Tue, 04 Jun 2019 00:15:02: 31000000 INFO @ Tue, 04 Jun 2019 00:15:09: 28000000 INFO @ Tue, 04 Jun 2019 00:15:09: 32000000 INFO @ Tue, 04 Jun 2019 00:15:09: 32000000 INFO @ Tue, 04 Jun 2019 00:15:17: 33000000 INFO @ Tue, 04 Jun 2019 00:15:17: 33000000 INFO @ Tue, 04 Jun 2019 00:15:18: 29000000 INFO @ Tue, 04 Jun 2019 00:15:24: 34000000 INFO @ Tue, 04 Jun 2019 00:15:24: 34000000 INFO @ Tue, 04 Jun 2019 00:15:26: 30000000 INFO @ Tue, 04 Jun 2019 00:15:31: 35000000 INFO @ Tue, 04 Jun 2019 00:15:32: 35000000 INFO @ Tue, 04 Jun 2019 00:15:35: 31000000 INFO @ Tue, 04 Jun 2019 00:15:37: #1 tag size is determined as 50 bps INFO @ Tue, 04 Jun 2019 00:15:37: #1 tag size = 50 INFO @ Tue, 04 Jun 2019 00:15:37: #1 total tags in treatment: 35764197 INFO @ Tue, 04 Jun 2019 00:15:37: #1 user defined the maximum tags... INFO @ Tue, 04 Jun 2019 00:15:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Jun 2019 00:15:38: #1 tags after filtering in treatment: 35764197 INFO @ Tue, 04 Jun 2019 00:15:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 04 Jun 2019 00:15:38: #1 finished! INFO @ Tue, 04 Jun 2019 00:15:38: #2 Build Peak Model... INFO @ Tue, 04 Jun 2019 00:15:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Jun 2019 00:15:40: #1 tag size is determined as 50 bps INFO @ Tue, 04 Jun 2019 00:15:40: #1 tag size = 50 INFO @ Tue, 04 Jun 2019 00:15:40: #1 total tags in treatment: 35764197 INFO @ Tue, 04 Jun 2019 00:15:40: #1 user defined the maximum tags... INFO @ Tue, 04 Jun 2019 00:15:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Jun 2019 00:15:41: #1 tags after filtering in treatment: 35764197 INFO @ Tue, 04 Jun 2019 00:15:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 04 Jun 2019 00:15:41: #1 finished! INFO @ Tue, 04 Jun 2019 00:15:41: #2 Build Peak Model... INFO @ Tue, 04 Jun 2019 00:15:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Jun 2019 00:15:41: #2 number of paired peaks: 42 WARNING @ Tue, 04 Jun 2019 00:15:41: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Jun 2019 00:15:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 04 Jun 2019 00:15:44: #2 number of paired peaks: 42 WARNING @ Tue, 04 Jun 2019 00:15:44: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Jun 2019 00:15:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 04 Jun 2019 00:15:44: 32000000 INFO @ Tue, 04 Jun 2019 00:15:53: 33000000 INFO @ Tue, 04 Jun 2019 00:16:01: 34000000 INFO @ Tue, 04 Jun 2019 00:16:09: 35000000 INFO @ Tue, 04 Jun 2019 00:16:15: #1 tag size is determined as 50 bps INFO @ Tue, 04 Jun 2019 00:16:15: #1 tag size = 50 INFO @ Tue, 04 Jun 2019 00:16:15: #1 total tags in treatment: 35764197 INFO @ Tue, 04 Jun 2019 00:16:15: #1 user defined the maximum tags... INFO @ Tue, 04 Jun 2019 00:16:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Jun 2019 00:16:16: #1 tags after filtering in treatment: 35764197 INFO @ Tue, 04 Jun 2019 00:16:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 04 Jun 2019 00:16:16: #1 finished! INFO @ Tue, 04 Jun 2019 00:16:16: #2 Build Peak Model... INFO @ Tue, 04 Jun 2019 00:16:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Jun 2019 00:16:19: #2 number of paired peaks: 42 WARNING @ Tue, 04 Jun 2019 00:16:19: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Jun 2019 00:16:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX751568/SRX751568.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。