Job ID = 6498756 SRX = SRX750079 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:53:37 prefetch.2.10.7: 1) Downloading 'SRR1638770'... 2020-06-25T23:53:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:55:19 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:55:19 prefetch.2.10.7: 1) 'SRR1638770' was downloaded successfully Read 18173870 spots for SRR1638770/SRR1638770.sra Written 18173870 spots for SRR1638770/SRR1638770.sra 2020-06-25T23:56:35 prefetch.2.10.7: 1) Downloading 'SRR1638771'... 2020-06-25T23:56:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:00:01 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:00:01 prefetch.2.10.7: 1) 'SRR1638771' was downloaded successfully Read 27599776 spots for SRR1638771/SRR1638771.sra Written 27599776 spots for SRR1638771/SRR1638771.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:56 45773646 reads; of these: 45773646 (100.00%) were unpaired; of these: 1756269 (3.84%) aligned 0 times 29992914 (65.52%) aligned exactly 1 time 14024463 (30.64%) aligned >1 times 96.16% overall alignment rate Time searching: 00:21:56 Overall time: 00:21:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 17580188 / 44017377 = 0.3994 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:36:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:36:11: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:36:11: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:36:17: 1000000 INFO @ Fri, 26 Jun 2020 09:36:23: 2000000 INFO @ Fri, 26 Jun 2020 09:36:29: 3000000 INFO @ Fri, 26 Jun 2020 09:36:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:36:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:36:41: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:36:41: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:36:41: 5000000 INFO @ Fri, 26 Jun 2020 09:36:47: 1000000 INFO @ Fri, 26 Jun 2020 09:36:47: 6000000 INFO @ Fri, 26 Jun 2020 09:36:53: 2000000 INFO @ Fri, 26 Jun 2020 09:36:53: 7000000 INFO @ Fri, 26 Jun 2020 09:36:59: 8000000 INFO @ Fri, 26 Jun 2020 09:36:59: 3000000 INFO @ Fri, 26 Jun 2020 09:37:05: 9000000 INFO @ Fri, 26 Jun 2020 09:37:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:37:11: 10000000 INFO @ Fri, 26 Jun 2020 09:37:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:37:11: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:37:11: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:37:11: 5000000 INFO @ Fri, 26 Jun 2020 09:37:17: 11000000 INFO @ Fri, 26 Jun 2020 09:37:17: 1000000 INFO @ Fri, 26 Jun 2020 09:37:18: 6000000 INFO @ Fri, 26 Jun 2020 09:37:23: 12000000 INFO @ Fri, 26 Jun 2020 09:37:23: 2000000 INFO @ Fri, 26 Jun 2020 09:37:24: 7000000 INFO @ Fri, 26 Jun 2020 09:37:29: 13000000 INFO @ Fri, 26 Jun 2020 09:37:30: 3000000 INFO @ Fri, 26 Jun 2020 09:37:30: 8000000 INFO @ Fri, 26 Jun 2020 09:37:35: 14000000 INFO @ Fri, 26 Jun 2020 09:37:36: 4000000 INFO @ Fri, 26 Jun 2020 09:37:36: 9000000 INFO @ Fri, 26 Jun 2020 09:37:41: 15000000 INFO @ Fri, 26 Jun 2020 09:37:42: 5000000 INFO @ Fri, 26 Jun 2020 09:37:42: 10000000 INFO @ Fri, 26 Jun 2020 09:37:47: 16000000 INFO @ Fri, 26 Jun 2020 09:37:48: 6000000 INFO @ Fri, 26 Jun 2020 09:37:48: 11000000 INFO @ Fri, 26 Jun 2020 09:37:53: 17000000 INFO @ Fri, 26 Jun 2020 09:37:54: 12000000 INFO @ Fri, 26 Jun 2020 09:37:54: 7000000 INFO @ Fri, 26 Jun 2020 09:37:59: 18000000 INFO @ Fri, 26 Jun 2020 09:38:00: 13000000 INFO @ Fri, 26 Jun 2020 09:38:00: 8000000 INFO @ Fri, 26 Jun 2020 09:38:05: 19000000 INFO @ Fri, 26 Jun 2020 09:38:06: 9000000 INFO @ Fri, 26 Jun 2020 09:38:06: 14000000 INFO @ Fri, 26 Jun 2020 09:38:11: 20000000 INFO @ Fri, 26 Jun 2020 09:38:12: 10000000 INFO @ Fri, 26 Jun 2020 09:38:12: 15000000 INFO @ Fri, 26 Jun 2020 09:38:17: 21000000 INFO @ Fri, 26 Jun 2020 09:38:18: 11000000 INFO @ Fri, 26 Jun 2020 09:38:18: 16000000 INFO @ Fri, 26 Jun 2020 09:38:23: 22000000 INFO @ Fri, 26 Jun 2020 09:38:24: 12000000 INFO @ Fri, 26 Jun 2020 09:38:25: 17000000 INFO @ Fri, 26 Jun 2020 09:38:29: 23000000 INFO @ Fri, 26 Jun 2020 09:38:30: 13000000 INFO @ Fri, 26 Jun 2020 09:38:31: 18000000 INFO @ Fri, 26 Jun 2020 09:38:35: 24000000 INFO @ Fri, 26 Jun 2020 09:38:36: 14000000 INFO @ Fri, 26 Jun 2020 09:38:37: 19000000 INFO @ Fri, 26 Jun 2020 09:38:41: 25000000 INFO @ Fri, 26 Jun 2020 09:38:42: 15000000 INFO @ Fri, 26 Jun 2020 09:38:43: 20000000 INFO @ Fri, 26 Jun 2020 09:38:47: 26000000 INFO @ Fri, 26 Jun 2020 09:38:48: 16000000 INFO @ Fri, 26 Jun 2020 09:38:49: 21000000 INFO @ Fri, 26 Jun 2020 09:38:50: #1 tag size is determined as 67 bps INFO @ Fri, 26 Jun 2020 09:38:50: #1 tag size = 67 INFO @ Fri, 26 Jun 2020 09:38:50: #1 total tags in treatment: 26437189 INFO @ Fri, 26 Jun 2020 09:38:50: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:38:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:38:51: #1 tags after filtering in treatment: 26437189 INFO @ Fri, 26 Jun 2020 09:38:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:38:51: #1 finished! INFO @ Fri, 26 Jun 2020 09:38:51: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:38:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:38:52: #2 number of paired peaks: 89 WARNING @ Fri, 26 Jun 2020 09:38:52: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:38:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:38:54: 17000000 INFO @ Fri, 26 Jun 2020 09:38:55: 22000000 INFO @ Fri, 26 Jun 2020 09:39:00: 18000000 INFO @ Fri, 26 Jun 2020 09:39:01: 23000000 INFO @ Fri, 26 Jun 2020 09:39:06: 19000000 INFO @ Fri, 26 Jun 2020 09:39:07: 24000000 INFO @ Fri, 26 Jun 2020 09:39:12: 20000000 INFO @ Fri, 26 Jun 2020 09:39:13: 25000000 INFO @ Fri, 26 Jun 2020 09:39:18: 21000000 INFO @ Fri, 26 Jun 2020 09:39:19: 26000000 INFO @ Fri, 26 Jun 2020 09:39:22: #1 tag size is determined as 67 bps INFO @ Fri, 26 Jun 2020 09:39:22: #1 tag size = 67 INFO @ Fri, 26 Jun 2020 09:39:22: #1 total tags in treatment: 26437189 INFO @ Fri, 26 Jun 2020 09:39:22: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:39:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:39:22: #1 tags after filtering in treatment: 26437189 INFO @ Fri, 26 Jun 2020 09:39:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:39:22: #1 finished! INFO @ Fri, 26 Jun 2020 09:39:22: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:39:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:39:24: #2 number of paired peaks: 89 WARNING @ Fri, 26 Jun 2020 09:39:24: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:39:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:39:24: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:39:30: 23000000 INFO @ Fri, 26 Jun 2020 09:39:36: 24000000 INFO @ Fri, 26 Jun 2020 09:39:42: 25000000 INFO @ Fri, 26 Jun 2020 09:39:48: 26000000 INFO @ Fri, 26 Jun 2020 09:39:51: #1 tag size is determined as 67 bps INFO @ Fri, 26 Jun 2020 09:39:51: #1 tag size = 67 INFO @ Fri, 26 Jun 2020 09:39:51: #1 total tags in treatment: 26437189 INFO @ Fri, 26 Jun 2020 09:39:51: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:39:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:39:51: #1 tags after filtering in treatment: 26437189 INFO @ Fri, 26 Jun 2020 09:39:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:39:51: #1 finished! INFO @ Fri, 26 Jun 2020 09:39:51: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:39:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:39:53: #2 number of paired peaks: 89 WARNING @ Fri, 26 Jun 2020 09:39:53: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:39:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750079/SRX750079.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。