Job ID = 6498715 SRX = SRX749027 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-26T00:01:10 prefetch.2.10.7: 1) Downloading 'SRR1636755'... 2020-06-26T00:01:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:10:39 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:10:39 prefetch.2.10.7: 1) 'SRR1636755' was downloaded successfully Read 46690539 spots for SRR1636755/SRR1636755.sra Written 46690539 spots for SRR1636755/SRR1636755.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:53:45 46690539 reads; of these: 46690539 (100.00%) were paired; of these: 4544566 (9.73%) aligned concordantly 0 times 25772049 (55.20%) aligned concordantly exactly 1 time 16373924 (35.07%) aligned concordantly >1 times ---- 4544566 pairs aligned concordantly 0 times; of these: 101947 (2.24%) aligned discordantly 1 time ---- 4442619 pairs aligned 0 times concordantly or discordantly; of these: 8885238 mates make up the pairs; of these: 7021250 (79.02%) aligned 0 times 1141801 (12.85%) aligned exactly 1 time 722187 (8.13%) aligned >1 times 92.48% overall alignment rate Time searching: 01:53:47 Overall time: 01:53:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6714374 / 42147340 = 0.1593 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 11:35:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 11:35:27: #1 read tag files... INFO @ Fri, 26 Jun 2020 11:35:27: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 11:35:34: 1000000 INFO @ Fri, 26 Jun 2020 11:35:42: 2000000 INFO @ Fri, 26 Jun 2020 11:35:49: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 11:35:56: 4000000 INFO @ Fri, 26 Jun 2020 11:35:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 11:35:56: #1 read tag files... INFO @ Fri, 26 Jun 2020 11:35:56: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 11:36:03: 1000000 INFO @ Fri, 26 Jun 2020 11:36:04: 5000000 INFO @ Fri, 26 Jun 2020 11:36:10: 2000000 INFO @ Fri, 26 Jun 2020 11:36:11: 6000000 INFO @ Fri, 26 Jun 2020 11:36:17: 3000000 INFO @ Fri, 26 Jun 2020 11:36:19: 7000000 INFO @ Fri, 26 Jun 2020 11:36:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 11:36:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 11:36:27: #1 read tag files... INFO @ Fri, 26 Jun 2020 11:36:27: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 11:36:27: 8000000 INFO @ Fri, 26 Jun 2020 11:36:31: 5000000 INFO @ Fri, 26 Jun 2020 11:36:34: 1000000 INFO @ Fri, 26 Jun 2020 11:36:35: 9000000 INFO @ Fri, 26 Jun 2020 11:36:38: 6000000 INFO @ Fri, 26 Jun 2020 11:36:42: 2000000 INFO @ Fri, 26 Jun 2020 11:36:42: 10000000 INFO @ Fri, 26 Jun 2020 11:36:45: 7000000 INFO @ Fri, 26 Jun 2020 11:36:50: 3000000 INFO @ Fri, 26 Jun 2020 11:36:50: 11000000 INFO @ Fri, 26 Jun 2020 11:36:52: 8000000 INFO @ Fri, 26 Jun 2020 11:36:57: 4000000 INFO @ Fri, 26 Jun 2020 11:36:58: 12000000 INFO @ Fri, 26 Jun 2020 11:36:58: 9000000 INFO @ Fri, 26 Jun 2020 11:37:05: 5000000 INFO @ Fri, 26 Jun 2020 11:37:05: 10000000 INFO @ Fri, 26 Jun 2020 11:37:05: 13000000 INFO @ Fri, 26 Jun 2020 11:37:12: 11000000 INFO @ Fri, 26 Jun 2020 11:37:13: 6000000 INFO @ Fri, 26 Jun 2020 11:37:13: 14000000 INFO @ Fri, 26 Jun 2020 11:37:19: 12000000 INFO @ Fri, 26 Jun 2020 11:37:21: 7000000 INFO @ Fri, 26 Jun 2020 11:37:21: 15000000 INFO @ Fri, 26 Jun 2020 11:37:26: 13000000 INFO @ Fri, 26 Jun 2020 11:37:29: 8000000 INFO @ Fri, 26 Jun 2020 11:37:29: 16000000 INFO @ Fri, 26 Jun 2020 11:37:33: 14000000 INFO @ Fri, 26 Jun 2020 11:37:36: 17000000 INFO @ Fri, 26 Jun 2020 11:37:37: 9000000 INFO @ Fri, 26 Jun 2020 11:37:40: 15000000 INFO @ Fri, 26 Jun 2020 11:37:44: 18000000 INFO @ Fri, 26 Jun 2020 11:37:45: 10000000 INFO @ Fri, 26 Jun 2020 11:37:47: 16000000 INFO @ Fri, 26 Jun 2020 11:37:52: 19000000 INFO @ Fri, 26 Jun 2020 11:37:53: 11000000 INFO @ Fri, 26 Jun 2020 11:37:54: 17000000 INFO @ Fri, 26 Jun 2020 11:38:00: 20000000 INFO @ Fri, 26 Jun 2020 11:38:00: 18000000 INFO @ Fri, 26 Jun 2020 11:38:01: 12000000 INFO @ Fri, 26 Jun 2020 11:38:07: 21000000 INFO @ Fri, 26 Jun 2020 11:38:07: 19000000 INFO @ Fri, 26 Jun 2020 11:38:09: 13000000 INFO @ Fri, 26 Jun 2020 11:38:14: 20000000 INFO @ Fri, 26 Jun 2020 11:38:15: 22000000 INFO @ Fri, 26 Jun 2020 11:38:17: 14000000 INFO @ Fri, 26 Jun 2020 11:38:22: 21000000 INFO @ Fri, 26 Jun 2020 11:38:23: 23000000 INFO @ Fri, 26 Jun 2020 11:38:25: 15000000 INFO @ Fri, 26 Jun 2020 11:38:29: 22000000 INFO @ Fri, 26 Jun 2020 11:38:31: 24000000 INFO @ Fri, 26 Jun 2020 11:38:33: 16000000 INFO @ Fri, 26 Jun 2020 11:38:36: 23000000 INFO @ Fri, 26 Jun 2020 11:38:39: 25000000 INFO @ Fri, 26 Jun 2020 11:38:40: 17000000 INFO @ Fri, 26 Jun 2020 11:38:43: 24000000 INFO @ Fri, 26 Jun 2020 11:38:47: 26000000 INFO @ Fri, 26 Jun 2020 11:38:48: 18000000 INFO @ Fri, 26 Jun 2020 11:38:50: 25000000 INFO @ Fri, 26 Jun 2020 11:38:56: 27000000 INFO @ Fri, 26 Jun 2020 11:38:56: 19000000 INFO @ Fri, 26 Jun 2020 11:38:57: 26000000 INFO @ Fri, 26 Jun 2020 11:39:04: 20000000 INFO @ Fri, 26 Jun 2020 11:39:04: 28000000 INFO @ Fri, 26 Jun 2020 11:39:04: 27000000 INFO @ Fri, 26 Jun 2020 11:39:11: 28000000 INFO @ Fri, 26 Jun 2020 11:39:11: 21000000 INFO @ Fri, 26 Jun 2020 11:39:12: 29000000 INFO @ Fri, 26 Jun 2020 11:39:18: 29000000 INFO @ Fri, 26 Jun 2020 11:39:18: 22000000 INFO @ Fri, 26 Jun 2020 11:39:20: 30000000 INFO @ Fri, 26 Jun 2020 11:39:25: 30000000 INFO @ Fri, 26 Jun 2020 11:39:26: 23000000 INFO @ Fri, 26 Jun 2020 11:39:28: 31000000 INFO @ Fri, 26 Jun 2020 11:39:32: 31000000 INFO @ Fri, 26 Jun 2020 11:39:33: 24000000 INFO @ Fri, 26 Jun 2020 11:39:36: 32000000 INFO @ Fri, 26 Jun 2020 11:39:39: 32000000 INFO @ Fri, 26 Jun 2020 11:39:41: 25000000 INFO @ Fri, 26 Jun 2020 11:39:43: 33000000 INFO @ Fri, 26 Jun 2020 11:39:46: 33000000 INFO @ Fri, 26 Jun 2020 11:39:49: 26000000 INFO @ Fri, 26 Jun 2020 11:39:51: 34000000 INFO @ Fri, 26 Jun 2020 11:39:53: 34000000 INFO @ Fri, 26 Jun 2020 11:39:56: 27000000 INFO @ Fri, 26 Jun 2020 11:39:59: 35000000 INFO @ Fri, 26 Jun 2020 11:40:00: 35000000 INFO @ Fri, 26 Jun 2020 11:40:04: 28000000 INFO @ Fri, 26 Jun 2020 11:40:07: 36000000 INFO @ Fri, 26 Jun 2020 11:40:07: 36000000 INFO @ Fri, 26 Jun 2020 11:40:12: 29000000 INFO @ Fri, 26 Jun 2020 11:40:15: 37000000 INFO @ Fri, 26 Jun 2020 11:40:15: 37000000 INFO @ Fri, 26 Jun 2020 11:40:20: 30000000 INFO @ Fri, 26 Jun 2020 11:40:23: 38000000 INFO @ Fri, 26 Jun 2020 11:40:23: 38000000 INFO @ Fri, 26 Jun 2020 11:40:27: 31000000 INFO @ Fri, 26 Jun 2020 11:40:31: 39000000 INFO @ Fri, 26 Jun 2020 11:40:32: 39000000 INFO @ Fri, 26 Jun 2020 11:40:35: 32000000 INFO @ Fri, 26 Jun 2020 11:40:38: 40000000 INFO @ Fri, 26 Jun 2020 11:40:40: 40000000 INFO @ Fri, 26 Jun 2020 11:40:43: 33000000 INFO @ Fri, 26 Jun 2020 11:40:45: 41000000 INFO @ Fri, 26 Jun 2020 11:40:48: 41000000 INFO @ Fri, 26 Jun 2020 11:40:50: 34000000 INFO @ Fri, 26 Jun 2020 11:40:52: 42000000 INFO @ Fri, 26 Jun 2020 11:40:56: 42000000 INFO @ Fri, 26 Jun 2020 11:40:58: 35000000 INFO @ Fri, 26 Jun 2020 11:40:59: 43000000 INFO @ Fri, 26 Jun 2020 11:41:04: 43000000 INFO @ Fri, 26 Jun 2020 11:41:06: 44000000 INFO @ Fri, 26 Jun 2020 11:41:06: 36000000 INFO @ Fri, 26 Jun 2020 11:41:11: 44000000 INFO @ Fri, 26 Jun 2020 11:41:12: 45000000 INFO @ Fri, 26 Jun 2020 11:41:14: 37000000 INFO @ Fri, 26 Jun 2020 11:41:19: 45000000 INFO @ Fri, 26 Jun 2020 11:41:19: 46000000 INFO @ Fri, 26 Jun 2020 11:41:22: 38000000 INFO @ Fri, 26 Jun 2020 11:41:26: 47000000 INFO @ Fri, 26 Jun 2020 11:41:27: 46000000 INFO @ Fri, 26 Jun 2020 11:41:30: 39000000 INFO @ Fri, 26 Jun 2020 11:41:33: 48000000 INFO @ Fri, 26 Jun 2020 11:41:35: 47000000 INFO @ Fri, 26 Jun 2020 11:41:38: 40000000 INFO @ Fri, 26 Jun 2020 11:41:40: 49000000 INFO @ Fri, 26 Jun 2020 11:41:43: 48000000 INFO @ Fri, 26 Jun 2020 11:41:46: 41000000 INFO @ Fri, 26 Jun 2020 11:41:47: 50000000 INFO @ Fri, 26 Jun 2020 11:41:51: 49000000 INFO @ Fri, 26 Jun 2020 11:41:54: 42000000 INFO @ Fri, 26 Jun 2020 11:41:54: 51000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 11:41:59: 50000000 INFO @ Fri, 26 Jun 2020 11:42:01: 52000000 INFO @ Fri, 26 Jun 2020 11:42:02: 43000000 INFO @ Fri, 26 Jun 2020 11:42:06: 51000000 INFO @ Fri, 26 Jun 2020 11:42:08: 53000000 INFO @ Fri, 26 Jun 2020 11:42:09: 44000000 INFO @ Fri, 26 Jun 2020 11:42:14: 52000000 INFO @ Fri, 26 Jun 2020 11:42:15: 54000000 INFO @ Fri, 26 Jun 2020 11:42:17: 45000000 INFO @ Fri, 26 Jun 2020 11:42:21: 53000000 INFO @ Fri, 26 Jun 2020 11:42:22: 55000000 INFO @ Fri, 26 Jun 2020 11:42:25: 46000000 INFO @ Fri, 26 Jun 2020 11:42:28: 56000000 INFO @ Fri, 26 Jun 2020 11:42:29: 54000000 INFO @ Fri, 26 Jun 2020 11:42:33: 47000000 INFO @ Fri, 26 Jun 2020 11:42:35: 57000000 INFO @ Fri, 26 Jun 2020 11:42:36: 55000000 INFO @ Fri, 26 Jun 2020 11:42:40: 48000000 INFO @ Fri, 26 Jun 2020 11:42:42: 58000000 INFO @ Fri, 26 Jun 2020 11:42:43: 56000000 INFO @ Fri, 26 Jun 2020 11:42:48: 49000000 INFO @ Fri, 26 Jun 2020 11:42:49: 59000000 INFO @ Fri, 26 Jun 2020 11:42:51: 57000000 INFO @ Fri, 26 Jun 2020 11:42:56: 50000000 INFO @ Fri, 26 Jun 2020 11:42:56: 60000000 INFO @ Fri, 26 Jun 2020 11:42:58: 58000000 INFO @ Fri, 26 Jun 2020 11:43:03: 61000000 INFO @ Fri, 26 Jun 2020 11:43:04: 51000000 INFO @ Fri, 26 Jun 2020 11:43:06: 59000000 INFO @ Fri, 26 Jun 2020 11:43:10: 62000000 INFO @ Fri, 26 Jun 2020 11:43:12: 52000000 INFO @ Fri, 26 Jun 2020 11:43:14: 60000000 INFO @ Fri, 26 Jun 2020 11:43:17: 63000000 INFO @ Fri, 26 Jun 2020 11:43:19: 53000000 INFO @ Fri, 26 Jun 2020 11:43:21: 61000000 INFO @ Fri, 26 Jun 2020 11:43:24: 64000000 INFO @ Fri, 26 Jun 2020 11:43:27: 54000000 INFO @ Fri, 26 Jun 2020 11:43:29: 62000000 INFO @ Fri, 26 Jun 2020 11:43:31: 65000000 INFO @ Fri, 26 Jun 2020 11:43:35: 55000000 INFO @ Fri, 26 Jun 2020 11:43:36: 63000000 INFO @ Fri, 26 Jun 2020 11:43:38: 66000000 INFO @ Fri, 26 Jun 2020 11:43:42: 56000000 INFO @ Fri, 26 Jun 2020 11:43:44: 64000000 INFO @ Fri, 26 Jun 2020 11:43:45: 67000000 INFO @ Fri, 26 Jun 2020 11:43:50: 57000000 INFO @ Fri, 26 Jun 2020 11:43:51: 65000000 INFO @ Fri, 26 Jun 2020 11:43:52: 68000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 11:43:57: 58000000 INFO @ Fri, 26 Jun 2020 11:43:59: 66000000 INFO @ Fri, 26 Jun 2020 11:43:59: 69000000 INFO @ Fri, 26 Jun 2020 11:44:05: 59000000 INFO @ Fri, 26 Jun 2020 11:44:06: 70000000 INFO @ Fri, 26 Jun 2020 11:44:06: 67000000 INFO @ Fri, 26 Jun 2020 11:44:13: 71000000 INFO @ Fri, 26 Jun 2020 11:44:13: 60000000 INFO @ Fri, 26 Jun 2020 11:44:14: 68000000 INFO @ Fri, 26 Jun 2020 11:44:20: 72000000 INFO @ Fri, 26 Jun 2020 11:44:21: 61000000 INFO @ Fri, 26 Jun 2020 11:44:21: 69000000 INFO @ Fri, 26 Jun 2020 11:44:27: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 11:44:27: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 11:44:27: #1 total tags in treatment: 35434970 INFO @ Fri, 26 Jun 2020 11:44:27: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 11:44:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 11:44:27: #1 tags after filtering in treatment: 30750228 INFO @ Fri, 26 Jun 2020 11:44:27: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 11:44:27: #1 finished! INFO @ Fri, 26 Jun 2020 11:44:27: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 11:44:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 11:44:29: 70000000 INFO @ Fri, 26 Jun 2020 11:44:29: 62000000 INFO @ Fri, 26 Jun 2020 11:44:29: #2 number of paired peaks: 202 WARNING @ Fri, 26 Jun 2020 11:44:29: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Fri, 26 Jun 2020 11:44:29: start model_add_line... INFO @ Fri, 26 Jun 2020 11:44:30: start X-correlation... INFO @ Fri, 26 Jun 2020 11:44:30: end of X-cor INFO @ Fri, 26 Jun 2020 11:44:30: #2 finished! INFO @ Fri, 26 Jun 2020 11:44:30: #2 predicted fragment length is 119 bps INFO @ Fri, 26 Jun 2020 11:44:30: #2 alternative fragment length(s) may be 2,64,119 bps INFO @ Fri, 26 Jun 2020 11:44:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.10_model.r INFO @ Fri, 26 Jun 2020 11:44:30: #3 Call peaks... INFO @ Fri, 26 Jun 2020 11:44:30: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 11:44:36: 71000000 INFO @ Fri, 26 Jun 2020 11:44:36: 63000000 INFO @ Fri, 26 Jun 2020 11:44:43: 72000000 INFO @ Fri, 26 Jun 2020 11:44:44: 64000000 INFO @ Fri, 26 Jun 2020 11:44:50: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 11:44:50: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 11:44:50: #1 total tags in treatment: 35434970 INFO @ Fri, 26 Jun 2020 11:44:50: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 11:44:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 11:44:51: #1 tags after filtering in treatment: 30750228 INFO @ Fri, 26 Jun 2020 11:44:51: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 11:44:51: #1 finished! INFO @ Fri, 26 Jun 2020 11:44:51: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 11:44:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 11:44:52: 65000000 INFO @ Fri, 26 Jun 2020 11:44:53: #2 number of paired peaks: 202 WARNING @ Fri, 26 Jun 2020 11:44:53: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Fri, 26 Jun 2020 11:44:53: start model_add_line... INFO @ Fri, 26 Jun 2020 11:44:53: start X-correlation... INFO @ Fri, 26 Jun 2020 11:44:53: end of X-cor INFO @ Fri, 26 Jun 2020 11:44:53: #2 finished! INFO @ Fri, 26 Jun 2020 11:44:53: #2 predicted fragment length is 119 bps INFO @ Fri, 26 Jun 2020 11:44:53: #2 alternative fragment length(s) may be 2,64,119 bps INFO @ Fri, 26 Jun 2020 11:44:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.05_model.r INFO @ Fri, 26 Jun 2020 11:44:53: #3 Call peaks... INFO @ Fri, 26 Jun 2020 11:44:53: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 11:44:59: 66000000 INFO @ Fri, 26 Jun 2020 11:45:07: 67000000 INFO @ Fri, 26 Jun 2020 11:45:14: 68000000 INFO @ Fri, 26 Jun 2020 11:45:21: 69000000 INFO @ Fri, 26 Jun 2020 11:45:28: 70000000 INFO @ Fri, 26 Jun 2020 11:45:32: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 11:45:35: 71000000 INFO @ Fri, 26 Jun 2020 11:45:42: 72000000 INFO @ Fri, 26 Jun 2020 11:45:49: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 11:45:49: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 11:45:49: #1 total tags in treatment: 35434970 INFO @ Fri, 26 Jun 2020 11:45:49: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 11:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 11:45:49: #1 tags after filtering in treatment: 30750228 INFO @ Fri, 26 Jun 2020 11:45:49: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 26 Jun 2020 11:45:49: #1 finished! INFO @ Fri, 26 Jun 2020 11:45:49: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 11:45:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 11:45:51: #2 number of paired peaks: 202 WARNING @ Fri, 26 Jun 2020 11:45:51: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Fri, 26 Jun 2020 11:45:51: start model_add_line... INFO @ Fri, 26 Jun 2020 11:45:52: start X-correlation... INFO @ Fri, 26 Jun 2020 11:45:52: end of X-cor INFO @ Fri, 26 Jun 2020 11:45:52: #2 finished! INFO @ Fri, 26 Jun 2020 11:45:52: #2 predicted fragment length is 119 bps INFO @ Fri, 26 Jun 2020 11:45:52: #2 alternative fragment length(s) may be 2,64,119 bps INFO @ Fri, 26 Jun 2020 11:45:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.20_model.r INFO @ Fri, 26 Jun 2020 11:45:52: #3 Call peaks... INFO @ Fri, 26 Jun 2020 11:45:52: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 11:45:56: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 11:46:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.10_peaks.xls INFO @ Fri, 26 Jun 2020 11:46:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 11:46:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.10_summits.bed INFO @ Fri, 26 Jun 2020 11:46:02: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4223 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 11:46:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.05_peaks.xls INFO @ Fri, 26 Jun 2020 11:46:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 11:46:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.05_summits.bed INFO @ Fri, 26 Jun 2020 11:46:26: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7742 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 11:46:54: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 11:47:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.20_peaks.xls INFO @ Fri, 26 Jun 2020 11:47:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 11:47:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX749027/SRX749027.20_summits.bed INFO @ Fri, 26 Jun 2020 11:47:23: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1667 records, 4 fields): 3 millis CompletedMACS2peakCalling