Job ID = 12266072 SRX = SRX7282669 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5700 spots for SRR10603084/SRR10603084.sra Written 5700 spots for SRR10603084/SRR10603084.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266180 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 5700 reads; of these: 5700 (100.00%) were unpaired; of these: 652 (11.44%) aligned 0 times 165 (2.89%) aligned exactly 1 time 4883 (85.67%) aligned >1 times 88.56% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 4236 / 5048 = 0.8391 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:46:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:46:35: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:46:35: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:46:35: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:46:35: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:46:35: #1 total tags in treatment: 812 INFO @ Sat, 03 Apr 2021 08:46:35: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:46:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:46:35: #1 tags after filtering in treatment: 807 INFO @ Sat, 03 Apr 2021 08:46:35: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 03 Apr 2021 08:46:35: #1 finished! INFO @ Sat, 03 Apr 2021 08:46:35: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:46:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:46:35: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:46:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:46:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:47:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:47:05: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:47:05: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:47:05: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:47:05: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:47:05: #1 total tags in treatment: 812 INFO @ Sat, 03 Apr 2021 08:47:05: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:47:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:47:05: #1 tags after filtering in treatment: 807 INFO @ Sat, 03 Apr 2021 08:47:05: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 03 Apr 2021 08:47:05: #1 finished! INFO @ Sat, 03 Apr 2021 08:47:05: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:47:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:47:05: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:47:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:47:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:47:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:47:35: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:47:35: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:47:35: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:47:35: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:47:35: #1 total tags in treatment: 812 INFO @ Sat, 03 Apr 2021 08:47:35: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:47:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:47:35: #1 tags after filtering in treatment: 807 INFO @ Sat, 03 Apr 2021 08:47:35: #1 Redundant rate of treatment: 0.01 INFO @ Sat, 03 Apr 2021 08:47:35: #1 finished! INFO @ Sat, 03 Apr 2021 08:47:35: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:47:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:47:35: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:47:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:47:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282669/SRX7282669.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling