Job ID = 12265987 SRX = SRX7282623 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7021 spots for SRR10603070/SRR10603070.sra Written 7021 spots for SRR10603070/SRR10603070.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266096 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 7021 reads; of these: 7021 (100.00%) were unpaired; of these: 856 (12.19%) aligned 0 times 244 (3.48%) aligned exactly 1 time 5921 (84.33%) aligned >1 times 87.81% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 5305 / 6165 = 0.8605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:42:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:42:03: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:42:03: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:42:03: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:42:03: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:42:03: #1 total tags in treatment: 860 INFO @ Sat, 03 Apr 2021 08:42:03: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:42:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:42:03: #1 tags after filtering in treatment: 857 INFO @ Sat, 03 Apr 2021 08:42:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:42:03: #1 finished! INFO @ Sat, 03 Apr 2021 08:42:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:42:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:42:03: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:42:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:42:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:42:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:42:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:42:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:42:32: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:42:32: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:42:32: #1 total tags in treatment: 860 INFO @ Sat, 03 Apr 2021 08:42:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:42:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:42:32: #1 tags after filtering in treatment: 857 INFO @ Sat, 03 Apr 2021 08:42:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:42:32: #1 finished! INFO @ Sat, 03 Apr 2021 08:42:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:42:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:42:32: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:42:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:42:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:43:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:43:02: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:43:02: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:43:02: #1 tag size is determined as 54 bps INFO @ Sat, 03 Apr 2021 08:43:02: #1 tag size = 54 INFO @ Sat, 03 Apr 2021 08:43:02: #1 total tags in treatment: 860 INFO @ Sat, 03 Apr 2021 08:43:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:43:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:43:02: #1 tags after filtering in treatment: 857 INFO @ Sat, 03 Apr 2021 08:43:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:43:02: #1 finished! INFO @ Sat, 03 Apr 2021 08:43:02: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:43:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:43:02: #2 number of paired peaks: 0 WARNING @ Sat, 03 Apr 2021 08:43:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:43:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7282623/SRX7282623.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling