Job ID = 6626605 SRX = SRX7262537 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19508220 spots for SRR10582167/SRR10582167.sra Written 19508220 spots for SRR10582167/SRR10582167.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626730 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:13 19508220 reads; of these: 19508220 (100.00%) were unpaired; of these: 18263204 (93.62%) aligned 0 times 1050251 (5.38%) aligned exactly 1 time 194765 (1.00%) aligned >1 times 6.38% overall alignment rate Time searching: 00:03:13 Overall time: 00:03:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 251552 / 1245016 = 0.2020 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:55:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:55:21: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:55:21: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:55:27: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:55:27: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:55:27: #1 total tags in treatment: 993464 INFO @ Tue, 14 Jul 2020 07:55:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:55:27: #1 tags after filtering in treatment: 993464 INFO @ Tue, 14 Jul 2020 07:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:55:27: #1 finished! INFO @ Tue, 14 Jul 2020 07:55:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:55:27: #2 number of paired peaks: 8358 INFO @ Tue, 14 Jul 2020 07:55:27: start model_add_line... INFO @ Tue, 14 Jul 2020 07:55:27: start X-correlation... INFO @ Tue, 14 Jul 2020 07:55:27: end of X-cor INFO @ Tue, 14 Jul 2020 07:55:27: #2 finished! INFO @ Tue, 14 Jul 2020 07:55:27: #2 predicted fragment length is 234 bps INFO @ Tue, 14 Jul 2020 07:55:27: #2 alternative fragment length(s) may be 234 bps INFO @ Tue, 14 Jul 2020 07:55:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.05_model.r INFO @ Tue, 14 Jul 2020 07:55:27: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:55:27: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:55:30: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:55:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.05_peaks.xls INFO @ Tue, 14 Jul 2020 07:55:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:55:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.05_summits.bed INFO @ Tue, 14 Jul 2020 07:55:31: Done! pass1 - making usageList (15 chroms): 0 millis pass2 - checking and writing primary data (2602 records, 4 fields): 11 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:55:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:55:51: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:55:51: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:55:57: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:55:57: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:55:57: #1 total tags in treatment: 993464 INFO @ Tue, 14 Jul 2020 07:55:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:55:57: #1 tags after filtering in treatment: 993464 INFO @ Tue, 14 Jul 2020 07:55:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:55:57: #1 finished! INFO @ Tue, 14 Jul 2020 07:55:57: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:55:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:55:57: #2 number of paired peaks: 8358 INFO @ Tue, 14 Jul 2020 07:55:57: start model_add_line... INFO @ Tue, 14 Jul 2020 07:55:57: start X-correlation... INFO @ Tue, 14 Jul 2020 07:55:57: end of X-cor INFO @ Tue, 14 Jul 2020 07:55:57: #2 finished! INFO @ Tue, 14 Jul 2020 07:55:57: #2 predicted fragment length is 234 bps INFO @ Tue, 14 Jul 2020 07:55:57: #2 alternative fragment length(s) may be 234 bps INFO @ Tue, 14 Jul 2020 07:55:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.10_model.r INFO @ Tue, 14 Jul 2020 07:55:57: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:55:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:56:00: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:56:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.10_peaks.xls INFO @ Tue, 14 Jul 2020 07:56:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:56:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.10_summits.bed INFO @ Tue, 14 Jul 2020 07:56:01: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1346 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:56:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:56:21: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:56:21: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:56:27: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:56:27: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:56:27: #1 total tags in treatment: 993464 INFO @ Tue, 14 Jul 2020 07:56:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:56:27: #1 tags after filtering in treatment: 993464 INFO @ Tue, 14 Jul 2020 07:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:56:27: #1 finished! INFO @ Tue, 14 Jul 2020 07:56:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:56:27: #2 number of paired peaks: 8358 INFO @ Tue, 14 Jul 2020 07:56:27: start model_add_line... INFO @ Tue, 14 Jul 2020 07:56:27: start X-correlation... INFO @ Tue, 14 Jul 2020 07:56:27: end of X-cor INFO @ Tue, 14 Jul 2020 07:56:27: #2 finished! INFO @ Tue, 14 Jul 2020 07:56:27: #2 predicted fragment length is 234 bps INFO @ Tue, 14 Jul 2020 07:56:27: #2 alternative fragment length(s) may be 234 bps INFO @ Tue, 14 Jul 2020 07:56:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.20_model.r INFO @ Tue, 14 Jul 2020 07:56:27: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:56:27: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:56:30: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:56:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.20_peaks.xls INFO @ Tue, 14 Jul 2020 07:56:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:56:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7262537/SRX7262537.20_summits.bed INFO @ Tue, 14 Jul 2020 07:56:31: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (498 records, 4 fields): 11 millis CompletedMACS2peakCalling